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The map label for this gene is braB [H]

Identifier: 126698856

GI number: 126698856

Start: 1461531

End: 1462823

Strand: Direct

Name: braB [H]

Synonym: CD1259

Alternate gene names: 126698856

Gene position: 1461531-1462823 (Clockwise)

Preceding gene: 126698855

Following gene: 126698857

Centisome position: 34.07

GC content: 33.26

Gene sequence:

>1293_bases
ATGGGTAAGACCAAGGATGTTATAGTATTTGGATTTGCGTTATTTGCAATGTTCTTTGGAGCAGGTAACTTAATATTTCC
ACCTTACTTGGGAATAATTACAGGTCCAGAATGGTTAATTGCCTTCTTAGGATTTACGTTTGCTGATGCTGGATTGGCAC
TTCTAGCAGTAATGGCTACAGCTAAATTCGATGGAAACGTAGTTGAAATGTTTAAAAGATGTGGTATTAAGTTAGGAATT
CTTATAGGTTGTGCAGATATTTTATGTATAGGCCCATTCTTAGCTATACCAAGAACAGGTGCAACAACTTATGAAATGGG
TATAATGCCTTTGTTTGGAACTTCAATTCCAGTGTTATTATTCTGTATACTTTTTTTTGCGATTTCATATGTACTAACAA
TAAGACCTTCTAAGGTCGTAGATATAGTTGGACAGTTTTTAACTCCAGCTCTTCTAATAGCTTTAGCATTTATAATTATA
AAAGGTATTATATCACCTCTTGGAGATATAGTAGATAAACCTATGATACCTAATGTATTTGCAGAAGGTATAGGTCAAGG
ATATCAGACAATGGATGCTTTTGCAGCAATAGCACTTGCATCAGTGCTAATAGTATCTTTAAATGATAAAGGATATTCTA
CTATTAGTGACAAGTTGAAAATGATTGGTAAGGCGGGTGTTCTTGCTTGTGGAGGATTAGCTCTAGTTTATGGAGGATTA
TGTTTCCTTGGGGCAACTGTATCAACAATGTATGGAACAGATGCAGTACAATCACAAGTAATAGTAAATATAACTGAAGG
ATTGCTTGGAAATGTTGGGAAAGCGATTTTAGCAGTAGTAGTATCATTAGCATGTTTAACTACATCTATAGGTCTTACAT
CTGCCACAGGTCAATATTTCTCTAGACTTACAAAAGGTAAATTGAGTTATGAAAAAATAGTATTAGCTGTATCTGTATTC
AGTGCAGTAGTAGCATCTTTTGGTGTTGGAACTATAATAAAGATAGCATCTCCTATACTAAGTATAGTTTATCCACCATC
AATTGTTTTAATAATTTTAGCTTTCTTCAATGAAAAAATTAAGAATGATAATGTATACAAAGGAGCAGTATATATGTCAT
TGCTTGTAAGTATACTAACAGTTATATCTAGTTATGGAGTAGCTGTTCCAGTAGTAAATTCTTTACCTCTTAATTCATTA
GGATTTAACTGGGTGGTGCCAGTTATAATAGCTGGAATAATTGGTAATTTTATACCATCAAAGAGTCAATCTAATACACT
TGGAACAAACTAA

Upstream 100 bases:

>100_bases
TTAATAATAAGTTCTTATTGTTTAATTCAACTTACTTATGCAAGCGTAAACAATCGAGACATTTTATTAATTTAATAAGG
TTAAAAAGGGGGATTTTATA

Downstream 100 bases:

>100_bases
GTTTTGTTTAATTAAAATATAATCAATTGTAAATAAATGAGTTAACTAAAAGTAAAAGGAGTTATTTGTACAAATAACTC
CTTTTATTTTTAGATTCTAT

Product: branched-chain amino acid transport system carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLGFTFADAGLALLAVMATAKFDGNVVEMFKRCGIKLGI
LIGCADILCIGPFLAIPRTGATTYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDKLKMIGKAGVLACGGLALVYGGL
CFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAILAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVF
SAVVASFGVGTIIKIASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVVNSLPLNSL
GFNWVVPVIIAGIIGNFIPSKSQSNTLGTN

Sequences:

>Translated_430_residues
MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLGFTFADAGLALLAVMATAKFDGNVVEMFKRCGIKLGI
LIGCADILCIGPFLAIPRTGATTYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDKLKMIGKAGVLACGGLALVYGGL
CFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAILAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVF
SAVVASFGVGTIIKIASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVVNSLPLNSL
GFNWVVPVIIAGIIGNFIPSKSQSNTLGTN
>Mature_429_residues
GKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLGFTFADAGLALLAVMATAKFDGNVVEMFKRCGIKLGIL
IGCADILCIGPFLAIPRTGATTYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIIIK
GIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDKLKMIGKAGVLACGGLALVYGGLC
FLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAILAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFS
AVVASFGVGTIIKIASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVVNSLPLNSLG
FNWVVPVIIAGIIGNFIPSKSQSNTLGTN

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=415, Percent_Identity=32.5301204819277, Blast_Score=212, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 45096; Mature: 44964

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLGFTFADAGLALLAVMAT
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
AKFDGNVVEMFKRCGIKLGILIGCADILCIGPFLAIPRTGATTYEMGIMPLFGTSIPVLL
HCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHCCHHHHHH
FCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIIIKGIISPLGDIVDKPMIPNVF
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
AEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDKLKMIGKAGVLACGGLALVYGGL
HHHHCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
CFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAILAVVVSLACLTTSIGLTSATGQYF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIKIASPILSIVYPPSIVLIILAFFNEKI
HHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KNDNVYKGAVYMSLLVSILTVISSYGVAVPVVNSLPLNSLGFNWVVPVIIAGIIGNFIPS
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCC
KSQSNTLGTN
CCCCCCCCCC
>Mature Secondary Structure 
GKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLGFTFADAGLALLAVMAT
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
AKFDGNVVEMFKRCGIKLGILIGCADILCIGPFLAIPRTGATTYEMGIMPLFGTSIPVLL
HCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHCCHHHHHH
FCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIIIKGIISPLGDIVDKPMIPNVF
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
AEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDKLKMIGKAGVLACGGLALVYGGL
HHHHCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
CFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAILAVVVSLACLTTSIGLTSATGQYF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIKIASPILSIVYPPSIVLIILAFFNEKI
HHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KNDNVYKGAVYMSLLVSILTVISSYGVAVPVVNSLPLNSLGFNWVVPVIIAGIIGNFIPS
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCC
KSQSNTLGTN
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]