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The map label for this gene is braB [H]

Identifier: 126698857

GI number: 126698857

Start: 1463942

End: 1465234

Strand: Direct

Name: braB [H]

Synonym: CD1260

Alternate gene names: 126698857

Gene position: 1463942-1465234 (Clockwise)

Preceding gene: 126698856

Following gene: 126698858

Centisome position: 34.12

GC content: 33.33

Gene sequence:

>1293_bases
ATGGGTGAGAAACAAATCAATTTTAAAGATGTAATTGTAATTGGATTTGCACTATTTGCAATGTTTTTTGGTGCAGGGAA
CTTAATCTTCCCTCCATATCTAGGAGTTTTATCTGGAAGTAGTTGGCTAGTAGCTTTCATAGGATTCCTTTTTGCAGATG
GTGGTTTGGCATTATTAGCAGTTATAGCAGCAACTAAGTTCAATGGAGACACATCGAAGATGTTCTCAAGAGCAGGCAAA
GGATTAAGTATTGCATTAGGATGTGCAATGGTAATCTGTATAGGGCCACTTTTAGCAATACCTAGAACAGCAGCAACAAC
TTATGAAATGGGTATATTACCTACAATAGGTTCTGGAATAAGCCCAGTAATATTCTCTATAGTATTCTTTGCTCTAGTAT
TGGTTTTAACAATAAGACCATCAAAAGTTGTAGATATAGTTGGGTCAATATTAACTCCAGCTTTACTTATAGCTTTGGCA
GTATTAATAGTTAAAGGTATCGTATCTCCATTAGGAGAAATAAGAGACAGTTCTTTAATACAAAATGTATTTGCAGAAGG
TATAACTCAAGGTTATCAAACAATGGATGCATTGGCAGCTAGTGTATTTGCATCAATTATAATCATGTCAGTAATAGCTA
AAGGTTATACAGGTGAGAAAGAAAAGATGAAAGCCACAATAAGTGCAGGTGTAATTGCAGTAATAGGAATGGCATTAGTT
TATGGTGGATTATGTTATCTTGGAGCAACTGTGTCTCAACAATATGGACAAGATGTTCAACAAACAGCTTTAATAGTTGC
AATAACAGCAGCTTTACTTGGAACTACAGGTAAAATTTTATTGGCTATTATAGTTGCATTAGCATGTTTAACAACAGCTA
TAGGTCTTTCTTCAGCAGCTGGTCAATATTTCTCAACACTTACAGATGGAAAATTAAAGTATGAGCATATAGTTATAGTA
GTTTGTGTATTTAGTGCAATCATATCAAACTTTGGAGTAAGTACAATAATACAATTCTCTTCTCCTATACTAAGTATGGT
ATATCCAGCAACTATAACTCTAGTTATACTAGCATTATTTAGTAATAAAATTAAAAATGACAACGTGTTCAAATGTGCTG
CATACATGGCATTATTAGTAAGTGTATTAACAGTTGCAACAAGTTTTGGTGTAAAAATTCCTCTAGTTAATAGCTTACCA
TTTGCTTCATTAGGATTTAACTGGATAGTTCCAGTTGTAATAGCAGGAATAATAGGAAACTTTATTCCATCTAAAAGCAG
TAAAACTGCATAA

Upstream 100 bases:

>100_bases
GTATAATTAAATAAAATCTCGTCAAAAGGTTTTCATTTAATTAAGAGATTGGACTTATTTTTTAGTAAAAGGTAGTAAAA
TTCAAATGGGGGTGCTTTAA

Downstream 100 bases:

>100_bases
TAATAAAAACTAATTGATAATTATGGAATTGCCTTTAAATTATATAGGCAATTCCTTTTTATATTTAAAAGAATTATTAA
ATTATTGTAGATAAAAGAAC

Product: branched chain amino acid transport system carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLVAFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGK
GLSIALGCAMVICIGPLLAIPRTAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTGEKEKMKATISAGVIAVIGMALV
YGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTTGKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIV
VCVFSAIISNFGVSTIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVKIPLVNSLP
FASLGFNWIVPVVIAGIIGNFIPSKSSKTA

Sequences:

>Translated_430_residues
MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLVAFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGK
GLSIALGCAMVICIGPLLAIPRTAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTGEKEKMKATISAGVIAVIGMALV
YGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTTGKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIV
VCVFSAIISNFGVSTIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVKIPLVNSLP
FASLGFNWIVPVVIAGIIGNFIPSKSSKTA
>Mature_429_residues
GEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLVAFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKG
LSIALGCAMVICIGPLLAIPRTAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALAV
LIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTGEKEKMKATISAGVIAVIGMALVY
GGLCYLGATVSQQYGQDVQQTALIVAITAALLGTTGKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVV
CVFSAIISNFGVSTIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVKIPLVNSLPF
ASLGFNWIVPVVIAGIIGNFIPSKSSKTA

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=419, Percent_Identity=35.5608591885441, Blast_Score=237, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 44754; Mature: 44623

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCCCC
>Mature Secondary Structure 
GEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLVAFIGFLFADGGLALLA
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHH
VIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPRTAATTYEMGILPTIGSGI
HHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCH
SPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALAVLIVKGIVSPLGEIRDSSLI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTGEKEKMKATISAGVIAVIGMALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
YGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTTGKILLAIIVALACLTTAIGLSSAA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
GQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVSTIIQFSSPILSMVYPATITLVILALF
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNKIKNDNVFKCAAYMALLVSVLTVATSFGVKIPLVNSLPFASLGFNWIVPVVIAGIIGN
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECEECCCCHHHCCHHHHHHHHHHHHHHH
FIPSKSSKTA
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]