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The map label for this gene is metH [H]

Identifier: 126698307

GI number: 126698307

Start: 889217

End: 890023

Strand: Direct

Name: metH [H]

Synonym: CD0727

Alternate gene names: 126698307

Gene position: 889217-890023 (Clockwise)

Preceding gene: 126698306

Following gene: 126698308

Centisome position: 20.73

GC content: 34.2

Gene sequence:

>807_bases
ATGGAAAAATTTATGATTATAGGTGAGAGAATACATTGTATATCACCTTCAATAAGAAAAGCATTAGCAGAAAGAGACCC
AGCTCCAATCTTAAAAAGAGCAAAAGAGCAATTAGAAGCAGGAGCACACTATATAGATTTTAATATAGGACCTGCCGAAA
GAGATGGCGAAGAAATAATGACATGGGGAATCAAATTACTTCAATCTGAGTTCAACAATGTTCCTATAGCATTAGATACA
GCTAATAAAAAAGCTATCGAAGCTGGACTTAAAGTTTATGATAGAACTAATGCAAAACCAATAATAAACTCTGCTGATGC
TGGCTCAAGATTTGATTTAATAGATATAGCAGCTGAATACGAAGCAATGGTTATAGGTTTATGTGCAAAAGAAGGTATTC
CAAGAGATAATGATGAGCGTATGGCTTACTGCCAAGAAATATTAGAAAAAGGCTTAATGTTAGGAATGGAGCCAACTGAT
ATACTATTTGACCCATTATGCTTAGTTATAAAAGGTATGCAAGAAAAACAAGTAGAAGTTTTAGAAGCTATAAAAATGAT
GACTGAGATGGGACTTTTAACTACAGGAGGATTATCTAACGTATCTAATGGATGTCCTAAGCATGTTAGACCTGTTTTAG
ATAGTGCATTCTTAGCAATGGCAATGGCTAATGGATTTAGTTCAGCTATAATGAATCCATGTGACCCAGAATTAATGAAA
ACTGTAAAATCTTGTGACATAATCAACGGGGCATCTTTATATGCAGACTCTTTCTTAGAGTTAAATGAAGGTGGATTTGC
TTTCTAG

Upstream 100 bases:

>100_bases
TTACCTAAGTTCTTAAAAGGATACCAAGAAAAAGCATGCCAAACAAACTAATAAAATATTAACTAACTATTAATATTAAA
TTTAAAGGGGGACATTATAA

Downstream 100 bases:

>100_bases
GGTTTTTAGGGTATAGTTAATAAAAAATAGGTGTAAATATTAAAGGGGGAAATAGAATGAATCTATATAATATAATCTTT
ACAGGGTCAGAACAAGCTTT

Product: carbon monoxide dehydrogenase/acetyl-CoA synthase complex, methyltransferase subunit

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHYIDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDT
ANKKAIEAGLKVYDRTNAKPIINSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFLAMAMANGFSSAIMNPCDPELMK
TVKSCDIINGASLYADSFLELNEGGFAF

Sequences:

>Translated_268_residues
MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHYIDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDT
ANKKAIEAGLKVYDRTNAKPIINSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFLAMAMANGFSSAIMNPCDPELMK
TVKSCDIINGASLYADSFLELNEGGFAF
>Mature_268_residues
MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHYIDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDT
ANKKAIEAGLKVYDRTNAKPIINSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFLAMAMANGFSSAIMNPCDPELMK
TVKSCDIINGASLYADSFLELNEGGFAF

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=248, Percent_Identity=31.4516129032258, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1790450, Length=249, Percent_Identity=28.9156626506024, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 29382; Mature: 29382

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHYIDFNIGPAERDGEEIM
CCCEEEECCCHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHH
TWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPIINSADAGSRFDLIDIAAEY
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
EAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTDILFDPLCLVIKGMQEKQVEV
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHH
LEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFLAMAMANGFSSAIMNPCDPELMK
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TVKSCDIINGASLYADSFLELNEGGFAF
HHHHCCCCCCHHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHYIDFNIGPAERDGEEIM
CCCEEEECCCHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHH
TWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPIINSADAGSRFDLIDIAAEY
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
EAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTDILFDPLCLVIKGMQEKQVEV
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHH
LEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFLAMAMANGFSSAIMNPCDPELMK
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TVKSCDIINGASLYADSFLELNEGGFAF
HHHHCCCCCCHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]