Click here to switch to the map view.

The map label for this gene is yfkN [H]

Identifier: 126698269

GI number: 126698269

Start: 836028

End: 837932

Strand: Direct

Name: yfkN [H]

Synonym: CD0690

Alternate gene names: 126698269

Gene position: 836028-837932 (Clockwise)

Preceding gene: 126698268

Following gene: 126698270

Centisome position: 19.49

GC content: 30.71

Gene sequence:

>1905_bases
ATGAAAATAAAATTTATTATAGTAGCATCAATAATATTTTTAATAGTATTACTTGTGGGGTGTCAAAGTACTAAAGACAC
AGAAATCAATGTACTCGCCACAAGTGATTTACATTCAATTATTCCAGATAATTTAATTTCCTATGTAGAAGAGGAGAGAA
AATATGATGAGAATCTACTTATGGTAGATGCAGGAGATTTTTTTGACATGCAAAGTGGTGAGATGAATAAGTGGTTTACT
GGTCAAAAATTAGTAAGATTTAAAGATGGGATACCAGAGTACAAAAAAATAGCTGAACCACGTGAAGGAGAAGTTCCATT
GGCTAAAAAAATGGCAAAGCTGAAGTATGATGCAGTTACATTTGGGAATCATGAATTTGTATCTAATGACAAGCAGAGCC
TTGATAAATTGGTTTCTGATTTTAAAAATAACAATATACCATTAGTATCAGCAAACGTATATGAACAATCTGGAGAAAAC
TACGTAAAGCCATATGTAATGAAAAATATAAATACTGACAAGGGAACTGTAAAGGTTGGTATACTTGGTCTGACTATAAA
AGAAGTAGGGGAACGTTCAAGGTTTAAAAGTTTTGAACAAGATAAAAAGGCTAGAGAACTTGGGGAACAACCTCAATACA
AAGGGAAATTATATGCAAATGATTTAGTAGAAGATGCAAATAAGTGGGTAAAGGTTATGGAAAAAAAAGAAAAGCCAGAT
ATAATAGTTGCCATTGTACACTCAGGTGAAGAGCCAAAAAATCCTAAAAATCCAGGAAATAGAATAAAAGAACTAGCAAC
AACTGTAGAGGGAATAGATGCTATTGTAGCAGGTCATACACATGAAAAAATAGAACAGCATACATATAAAAATAATTCTG
GTGAAGAAGTAATAGTAACACAGCCTGGAGCTCATGGTAATAGTATATCAAAGATTAATTTTAAGTTGGATAAGAAGAAT
GATAAGTGGTTTATAAAAGACAAATATAGTGAATTAAAGGTTTTTGGAAAGGAAGTAAATATAATTACAACTTCTGGTCT
GAATCCAAAATTTTCAGATGAAATGGCAGTTAATTTGTTTAATGAAAGAGATAGAGATGTTCAACCTATATTTATAGATA
CTGGAGATTTTTTAAATACTAATACAAAAGAAATGACTAAGTGGGCTAAAGAGTGGCAATACAATGAAAATAAAGGCATC
TATAAAAAAATAAATGAATGGCCAATTGTATATCTTGGTTATGATGCTGTAGTACTTGGTAGCCATGACTTTACAGAGGA
TAAAGAGACTTTTGAGGAAAAAAAATCTACATTGGATTATATTATAGAAAACTTTGAAGAAATGAAGATACCAGTATTAT
CAGCTAATATATATGATGAATCTGGAGAAAATTATGTAAAACCTTATATAATTCATAATGTAGAAACTAGGGAAGGAAAT
ATAAAGGTTGGAGTATTAGGTCTTACTACTACTGATAAGGATGAAGAAGACTTAAGTAACTCAAGCTTAAGCAAAGACTC
AAAGACTTCGAAAATTCCTGAAGAAACAGGAAAGTTATATACTAATAATCTAGTAAAAGATGCAAGCGAATGGGTAAAAG
TTATGCAAAAGGAAAGTCCTGATGTGATTGTTGCAGTTGTACATTCAGATAATAATAGTACTCTAAAAAATCCAAGTAGT
GAAGTCAAAAAACTTGCAATGTCAGTAGATGGAATAGATGCTATTGTTGTAGGTCATACGAAAAATAAGGTAGAAGAACA
TATTTACAAAAATAAGTCTGGGGATGAGGTTATAGTAACACAATCTGGAGAGGATTGTTATTCAAAAATAAGTCTTGAAC
TAAGAAAAGAAAATGGCAAGTGGAATATCATAAACAAATATAGTAAAGCAATAAAAATGAAGTGA

Upstream 100 bases:

>100_bases
TATAGTTCTTTTCGTATAATAAATAGTACAATCTTATATGGTAAAGATAAAGTGTGATATTAAATTTTATTATCATCCAA
AATCTAAGGAGGAGTCTCTT

Downstream 100 bases:

>100_bases
ATTTATTGATATATAAACCATTATTAATACTGATTTTTATAATATAATAAATACTGATTCTACAATATAATTAACACTGA
TTTTTATAATATAATAAATA

Product: nucleotide phosphodiesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 634; Mature: 634

Protein sequence:

>634_residues
MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDNLISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFT
GQKLVRFKDGIPEYKKIAEPREGEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLYANDLVEDANKWVKVMEKKEKPD
IIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIVAGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKN
DKWFIKDKYSELKVFGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEWQYNENKGI
YKKINEWPIVYLGYDAVVLGSHDFTEDKETFEEKKSTLDYIIENFEEMKIPVLSANIYDESGENYVKPYIIHNVETREGN
IKVGVLGLTTTDKDEEDLSNSSLSKDSKTSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSS
EVKKLAMSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNIINKYSKAIKMK

Sequences:

>Translated_634_residues
MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDNLISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFT
GQKLVRFKDGIPEYKKIAEPREGEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLYANDLVEDANKWVKVMEKKEKPD
IIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIVAGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKN
DKWFIKDKYSELKVFGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEWQYNENKGI
YKKINEWPIVYLGYDAVVLGSHDFTEDKETFEEKKSTLDYIIENFEEMKIPVLSANIYDESGENYVKPYIIHNVETREGN
IKVGVLGLTTTDKDEEDLSNSSLSKDSKTSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSS
EVKKLAMSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNIINKYSKAIKMK
>Mature_634_residues
MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDNLISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFT
GQKLVRFKDGIPEYKKIAEPREGEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLYANDLVEDANKWVKVMEKKEKPD
IIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIVAGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKN
DKWFIKDKYSELKVFGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEWQYNENKGI
YKKINEWPIVYLGYDAVVLGSHDFTEDKETFEEKKSTLDYIIENFEEMKIPVLSANIYDESGENYVKPYIIHNVETREGN
IKVGVLGLTTTDKDEEDLSNSSLSKDSKTSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSS
EVKKLAMSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNIINKYSKAIKMK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=362, Percent_Identity=21.5469613259669, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1786687, Length=215, Percent_Identity=28.3720930232558, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 72023; Mature: 72023

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00785 5_NUCLEOTIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDNLISYVEEERKYDENLL
CEEEEEHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEE
MVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPREGEVPLAKKMAKLKYDAVT
EEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHEEEEE
FGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGENYVKPYVMKNINTDKGTVKVG
CCCCHHHCCCHHHHHHHHHHHCCCCCCEEECHHHHCCCCCCCCCCEEECCCCCCCEEEEE
ILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLYANDLVEDANKWVKVMEKKEKPD
EEEEEHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
IIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIVAGHTHEKIEQHTYKNNSGEEVIVT
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEE
QPGAHGNSISKINFKLDKKNDKWFIKDKYSELKVFGKEVNIITTSGLNPKFSDEMAVNLF
CCCCCCCCEEEEEEEEECCCCEEEEECCHHHHHEECCEEEEEEECCCCCCCCCHHHHHHH
NERDRDVQPIFIDTGDFLNTNTKEMTKWAKEWQYNENKGIYKKINEWPIVYLGYDAVVLG
CCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEEECCEEEEC
SHDFTEDKETFEEKKSTLDYIIENFEEMKIPVLSANIYDESGENYVKPYIIHNVETREGN
CCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCCCCEEEEEEEECCCC
IKVGVLGLTTTDKDEEDLSNSSLSKDSKTSKIPEETGKLYTNNLVKDASEWVKVMQKESP
EEEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCC
DVIVAVVHSDNNSTLKNPSSEVKKLAMSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVT
CEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEE
QSGEDCYSKISLELRKENGKWNIINKYSKAIKMK
CCCHHHHHHEEEEEEECCCCEEEEHHHHHHHCCC
>Mature Secondary Structure
MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDNLISYVEEERKYDENLL
CEEEEEHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEE
MVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPREGEVPLAKKMAKLKYDAVT
EEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHEEEEE
FGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGENYVKPYVMKNINTDKGTVKVG
CCCCHHHCCCHHHHHHHHHHHCCCCCCEEECHHHHCCCCCCCCCCEEECCCCCCCEEEEE
ILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLYANDLVEDANKWVKVMEKKEKPD
EEEEEHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
IIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIVAGHTHEKIEQHTYKNNSGEEVIVT
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEE
QPGAHGNSISKINFKLDKKNDKWFIKDKYSELKVFGKEVNIITTSGLNPKFSDEMAVNLF
CCCCCCCCEEEEEEEEECCCCEEEEECCHHHHHEECCEEEEEEECCCCCCCCCHHHHHHH
NERDRDVQPIFIDTGDFLNTNTKEMTKWAKEWQYNENKGIYKKINEWPIVYLGYDAVVLG
CCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEEECCEEEEC
SHDFTEDKETFEEKKSTLDYIIENFEEMKIPVLSANIYDESGENYVKPYIIHNVETREGN
CCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCCCCEEEEEEEECCCC
IKVGVLGLTTTDKDEEDLSNSSLSKDSKTSKIPEETGKLYTNNLVKDASEWVKVMQKESP
EEEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCC
DVIVAVVHSDNNSTLKNPSSEVKKLAMSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVT
CEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEE
QSGEDCYSKISLELRKENGKWNIINKYSKAIKMK
CCCHHHHHHEEEEEEECCCCEEEEHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]