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The map label for this gene is yfkN [H]

Identifier: 126698268

GI number: 126698268

Start: 833702

End: 835627

Strand: Direct

Name: yfkN [H]

Synonym: CD0689

Alternate gene names: 126698268

Gene position: 833702-835627 (Clockwise)

Preceding gene: 126698267

Following gene: 126698269

Centisome position: 19.43

GC content: 31.0

Gene sequence:

>1926_bases
ATGAAAATAAAATTTGTTATTATGCCATTGGTGATATTTTTAATGGTATTATTTGTGGGGTGTCAAAGTACAAAAGATAC
AGAAGTTAAAGCAAAAGATACAGAAATTAAAATATTAGGGACAGGTGATTTACATTCAATTTTGACAGACTCTATGGTTT
CATATGTAAATGAGGAAAGGGAAAAGAATAAAAATCTGCTTATGGTAGATGCGGGAGATTTCTATGGTACACAAAGTAGA
GAAATGTGGGAATGGTCTTCTGGAAAAAAGTTAATTAATATTAAAAGAAATGGTAGAGCAGAGTATGATGACATAATTAA
GAGTAGTAAAGATGAAGTACCTATAGTTAAAGATATGGCAAAGTTGAAATATGATGCTGTAGTTTTGGGAGATAATGAAT
TTGTTTCAAATGATAAGCAAAGTCTTGATAAACTGGTTTCTGATTTTAAAAATAATAATATGCCATTGGTATCAGCAAAT
ATATATGAACAATCTGGAGAAAATTATGTACAACCATATGTAATGAAAAATATTAAGACAGATGAGGGCAATGTAAAAGT
TGGAATACTAGGTCTTACTATAAAAGAAGTAGGGGAAACCCTTGGTCTTGAGGATTTTGAAAAGGATAAAAAAGCTAGAG
AGCTTGGAGAACAAGCTGATTACAAAGGGAAATTATATGCAAATGATTTAGTAGAAGATGCAAAGAAATGGATAAAAGTT
ATGGAAAAAGAAAGTCCAGACATAATAGTTGCAGTTGTACACTCAGGGGAAGAACCTAAAACAGATAGAAATCCAGGAAA
TAGAATAAAAGAGTTAGCAACTACAGTGGATGGAATAGATGCAATTGTAGCTGGTCATACTCATGAAAAAATAGATGAAC
ACAAATATAAAAATAAATCTGGAGATGAAGTAATTGTTACACAACCAGGTTGGCATGGAGATAGAGTATCAGAGATTAAT
TTCAAATTAAATAAAGAGAATGGAAAGTGGGACATAAAGGAAAAGTCTAGTAAATTAAAAATAATTGATAAAGAAGTAAG
CATAGTTGCTACTGCAGATTTACATTCACATTTCTCTGATAAATTAACAGTTGACCTTGTTAATGAACGTAGTAACCCAA
TTGAACCTGTTGTAGTAGATGCAGGCGACTTCTTAGACCCTCAAACAGATGAAATGACTCAATGGTATAAAGAGTGGAAA
AGTATAAGAGATAATAATAGTGATAAAACGATTAGTAGATGCCCAATGGTCATGGATATGAATCAGGGAATGTATGATGC
AGTAGTACTTGGAAATCATGAATTTGTATCTGAAAAAGATGAATTTTGGTCAGATGAACGCCTTTTAGATGCTGTTATAG
AGGATTTTGAACAGGTGGCAATTTCTGTGTTATCGGCAAATATATATAGGCAATCTGGTAAAAATTATGTTAAACCATAT
ATTATAAAAGATGTGGAGACGAGTGAAGGTAATGTAAAAGTAGGAATATTGGGACTTACTATAAAAGAAGTAGGTAAAGG
TCTTAAAAATGTAAACTTACAAGAGCAATTTCAATATAAGGATAAATTGTATGCAAATGATTTAGTGGAAGATGCAAAGA
AATGGGTAAAAGTTATGAAAAAGAAAAAACCAGATATAATAGTTGCAGTTGTACATTCAGGAGAAGAACCAAAAAATCCA
AAACATCCAGGAAATAGAATAAAAGAACTAGCAACTACAGTAGATGGAATAGATGCAATTGTTGCTAGTCATACTCATGA
AAAAATAGATGAGCATGAATATAAAAATAAGTCTGGAGAAAAAGTTATAGTGACACAACCAGGTGAGCATGGTAAATATT
ATTCTAAAATTACCTTTAAAGTAAATAAAGAAAAGGGTTCGTGGATTATTAATGATAAATTCAGTAAAACAATTGAGGTT
GAATAA

Upstream 100 bases:

>100_bases
AATAAATGAATATTGCCCATAATCATATAAACTGCTATAGTTTAAAATAAAGCAGGACGTTAAATTTTATAGTAATCTAA
AAATTTAAGAGGGGGATTTT

Downstream 100 bases:

>100_bases
CTTTATAAAATTTATAGATAAAGTAAATTATCTGATATTGACATGATATATAAGATAAAGTTTCTAATATTAGTATAATG
CATAATGTGAAATATTTTAA

Product: nucleotide phosphodiesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDLHSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSR
EMWEWSSGKKLINIKRNGRAEYDDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQADYKGKLYANDLVEDAKKWIKV
MEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTVDGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEIN
FKLNKENGKWDIKEKSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEMTQWYKEWK
SIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLDAVIEDFEQVAISVLSANIYRQSGKNYVKPY
IIKDVETSEGNVKVGILGLTIKEVGKGLKNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNP
KHPGNRIKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKEKGSWIINDKFSKTIEV
E

Sequences:

>Translated_641_residues
MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDLHSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSR
EMWEWSSGKKLINIKRNGRAEYDDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQADYKGKLYANDLVEDAKKWIKV
MEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTVDGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEIN
FKLNKENGKWDIKEKSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEMTQWYKEWK
SIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLDAVIEDFEQVAISVLSANIYRQSGKNYVKPY
IIKDVETSEGNVKVGILGLTIKEVGKGLKNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNP
KHPGNRIKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKEKGSWIINDKFSKTIEV
E
>Mature_641_residues
MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDLHSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSR
EMWEWSSGKKLINIKRNGRAEYDDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQADYKGKLYANDLVEDAKKWIKV
MEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTVDGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEIN
FKLNKENGKWDIKEKSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEMTQWYKEWK
SIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLDAVIEDFEQVAISVLSANIYRQSGKNYVKPY
IIKDVETSEGNVKVGILGLTIKEVGKGLKNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNP
KHPGNRIKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKEKGSWIINDKFSKTIEV
E

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=345, Percent_Identity=21.4492753623188, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 72699; Mature: 72699

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00785 5_NUCLEOTIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDLHSILTDSMVSYVNEER
CEEEEEHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHH
EKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEYDDIIKSSKDEVPIVKDMA
HCCCCEEEEECCCCCCCCCCCCEECCCCCEEEEEECCCCCCHHHHHHCCCCCCCHHHHHH
KLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSANIYEQSGENYVQPYVMKNIKT
HCCCCEEEECCCHHHCCCHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCCCCCHHHHCCCC
DEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQADYKGKLYANDLVEDAKKWIKV
CCCCEEEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
MEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTVDGIDAIVAGHTHEKIDEHKYKNKS
HHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHCCCCC
GDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKEKSSKLKIIDKEVSIVATADLHSHFSD
CCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEECCEEEEEEEHHHHCCCC
KLTVDLVNERSNPIEPVVVDAGDFLDPQTDEMTQWYKEWKSIRDNNSDKTISRCPMVMDM
CEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCEEEEC
NQGMYDAVVLGNHEFVSEKDEFWSDERLLDAVIEDFEQVAISVLSANIYRQSGKNYVKPY
CCCCEEEEEECCCHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IIKDVETSEGNVKVGILGLTIKEVGKGLKNVNLQEQFQYKDKLYANDLVEDAKKWVKVMK
EEEEEECCCCCEEEEEEEEEHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKKPDIIVAVVHSGEEPKNPKHPGNRIKELATTVDGIDAIVASHTHEKIDEHEYKNKSGE
CCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KVIVTQPGEHGKYYSKITFKVNKEKGSWIINDKFSKTIEVE
EEEEECCCCCCCEEEEEEEEEECCCCCEEEECCCCCEEECC
>Mature Secondary Structure
MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDLHSILTDSMVSYVNEER
CEEEEEHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHH
EKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEYDDIIKSSKDEVPIVKDMA
HCCCCEEEEECCCCCCCCCCCCEECCCCCEEEEEECCCCCCHHHHHHCCCCCCCHHHHHH
KLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSANIYEQSGENYVQPYVMKNIKT
HCCCCEEEECCCHHHCCCHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCCCCCHHHHCCCC
DEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQADYKGKLYANDLVEDAKKWIKV
CCCCEEEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
MEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTVDGIDAIVAGHTHEKIDEHKYKNKS
HHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHCCCCC
GDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKEKSSKLKIIDKEVSIVATADLHSHFSD
CCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEECCEEEEEEEHHHHCCCC
KLTVDLVNERSNPIEPVVVDAGDFLDPQTDEMTQWYKEWKSIRDNNSDKTISRCPMVMDM
CEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCEEEEC
NQGMYDAVVLGNHEFVSEKDEFWSDERLLDAVIEDFEQVAISVLSANIYRQSGKNYVKPY
CCCCEEEEEECCCHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IIKDVETSEGNVKVGILGLTIKEVGKGLKNVNLQEQFQYKDKLYANDLVEDAKKWVKVMK
EEEEEECCCCCEEEEEEEEEHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKKPDIIVAVVHSGEEPKNPKHPGNRIKELATTVDGIDAIVASHTHEKIDEHEYKNKSGE
CCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KVIVTQPGEHGKYYSKITFKVNKEKGSWIINDKFSKTIEVE
EEEEECCCCCCCEEEEEEEEEECCCCCEEEECCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]