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The map label for this gene is gluD

Identifier: 126697752

GI number: 126697752

Start: 236792

End: 238057

Strand: Direct

Name: gluD

Synonym: CD0179

Alternate gene names: 126697752

Gene position: 236792-238057 (Clockwise)

Preceding gene: 126697751

Following gene: 126697753

Centisome position: 5.52

GC content: 34.36

Gene sequence:

>1266_bases
ATGTCAGGAAAAGATGTAAATGTCTTCGAGATGGCGCAATCTCAAGTAAAAAATGCATGTGATAAATTAGGTATGGAACC
AGCAGTTTATGAATTATTAAAAGAACCTATGAGAGTTATAGAGGTTTCAATTCCAGTAAAAATGGATGATGGTTCTATAA
AAACTTTTAAAGGATTTAGATCACAACATAATGATGCAGTAGGGCCAACAAAAGGTGGAATAAGATTCCATCAAAATGTT
TCAAGAGACGAAGTAAAAGCTTTATCTATATGGATGACTTTCAAGTGTTCTGTAACAGGTATACCATATGGTGGAGGTAA
AGGTGGAATAATAGTAGATCCATCAACTTTATCTCAAGGTGAATTAGAAAGATTAAGTAGAGGATACATAGATGGAATAT
ATAAATTAATAGGTGAGAAAGTTGACGTTCCTGCACCAGACGTAAATACTAATGGTCAAATAATGTCTTGGATGGTTGAT
GAGTACAATAAATTAACTGGACAAAGTTCTATAGGTGTTATAACTGGTAAGCCAGTTGAATTTGGTGGTTCTTTAGGAAG
AACAGCTGCTACTGGATTTGGTGTTGCTGTTACTGCAAGAGAAGCTGCTGCTAAATTAGGAATAGATATGAAAAAAGCTA
AAATAGCTGTTCAAGGTATAGGAAACGTTGGTTCTTATACAGTTCTTAACTGTGAAAAACTTGGTGGTACTGTTGTAGCT
ATGGCTGAATGGTGTAAATCAGAAGGTTCTTATGCTATATACAATGAAAATGGTTTAGATGGTCAAGCTATGTTAGATTA
TATGAAAGAACATGGTAACTTATTAAACTTCCCAGGAGCTAAGAGAATATCTTTAGAAGAGTTCTGGGCTTCAGATGTTG
ATATAGTAATACCAGCTGCATTAGAAAACTCTATAACTAAAGAAGTTGCTGAATCTATAAAAGCTAAATTAGTTTGTGAG
GCTGCTAATGGACCAACTACTCCAGAGGCTGATGAAGTATTTGCTGAAAGAGGAATAGTTCTTACTCCAGATATATTAAC
TAATGCTGGTGGAGTTACAGTTTCTTACTTCGAGTGGGTACAAAACTTATATGGATACTACTGGTCAGAAGAAGAAGTAG
AGCAAAAAGAAGAAATAGCTATGGTTAAAGCATTTGAATCTATTTGGAAAATCAAAGAAGAGTACAATGTTACAATGAGA
GAAGCTGCTTACATGCATTCAATTAAGAAAGTTGCTGAAGCTATGAAATTAAGAGGATGGTACTAA

Upstream 100 bases:

>100_bases
TTTGCAATAAAGTGTGCTATAATTAAACTGTAAATTTAGGAAATGGTTTTTTCTAAATGGAGTATACATAATTATAACGT
ATTTATAAGGGGGCTTTCAA

Downstream 100 bases:

>100_bases
GATTTACTCTTAATAAATAATAGGATATAATTATTTAAGATATAAAGGGGCTGTACATAGTACAGCCCTTAAATTATGTG
GCTTACAATGAAGTAAGCTA

Product: NAD-specific glutamate dehydrogenase

Products: NA

Alternate protein names: NAD-GDH

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSIPVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNV
SRDEVKALSIWMTFKCSVTGIPYGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQGIGNVGSYTVLNCEKLGGTVVA
MAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLNFPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCE
AANGPTTPEADEVFAERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIKEEYNVTMR
EAAYMHSIKKVAEAMKLRGWY

Sequences:

>Translated_421_residues
MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSIPVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNV
SRDEVKALSIWMTFKCSVTGIPYGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQGIGNVGSYTVLNCEKLGGTVVA
MAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLNFPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCE
AANGPTTPEADEVFAERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIKEEYNVTMR
EAAYMHSIKKVAEAMKLRGWY
>Mature_420_residues
SGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSIPVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVS
RDEVKALSIWMTFKCSVTGIPYGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVDE
YNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQGIGNVGSYTVLNCEKLGGTVVAM
AEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLNFPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEA
ANGPTTPEADEVFAERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIKEEYNVTMRE
AAYMHSIKKVAEAMKLRGWY

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family

Homologues:

Organism=Homo sapiens, GI31377775, Length=348, Percent_Identity=41.6666666666667, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI4885281, Length=348, Percent_Identity=41.0919540229885, Blast_Score=268, Evalue=8e-72,
Organism=Escherichia coli, GI1788059, Length=406, Percent_Identity=32.512315270936, Blast_Score=191, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI17544676, Length=464, Percent_Identity=34.9137931034483, Blast_Score=251, Evalue=6e-67,
Organism=Saccharomyces cerevisiae, GI6319256, Length=437, Percent_Identity=29.9771167048055, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6324951, Length=430, Percent_Identity=30.4651162790698, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45549226, Length=346, Percent_Identity=41.6184971098266, Blast_Score=265, Evalue=5e-71,
Organism=Drosophila melanogaster, GI45553475, Length=346, Percent_Identity=41.6184971098266, Blast_Score=265, Evalue=6e-71,
Organism=Drosophila melanogaster, GI24649283, Length=349, Percent_Identity=38.9684813753582, Blast_Score=248, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24649460, Length=285, Percent_Identity=40.3508771929825, Blast_Score=205, Evalue=4e-53,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DHE2_CLODI (P27346)

Other databases:

- EMBL:   M65250
- PIR:   S28829
- ProteinModelPortal:   P27346
- SMR:   P27346
- PhylomeDB:   P27346
- BRENDA:   1.4.1.2
- GO:   GO:0005488
- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11606:SF2
- PIRSF:   PIRSF000185
- PRINTS:   PR00082
- SMART:   SM00839

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N

EC number: =1.4.1.2

Molecular weight: Translated: 46016; Mature: 45885

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: ACT_SITE 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSIPVKMDDGSIKTFKGFR
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHH
SQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIPYGGGKGGIIVDPSTLSQG
HCCCCCCCCCCCCEEECCCCCHHHHEEEEEEEEEEEEEEECCCCCCCCCEEECCCCCCHH
ELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVDEYNKLTGQSSIGVITGKPVE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHH
FGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQGIGNVGSYTVLNCEKLGGTVVA
CCCCCCCHHHCCCCEEEEHHHHHHHHCCCHHHHEEEEEECCCCCCEEEEEHHHHCCHHHH
MAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLNFPGAKRISLEEFWASDVDIVIPAA
HHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCEECCCCCCCCCHHHHCCCCCCEEEEHH
LENSITKEVAESIKAKLVCEAANGPTTPEADEVFAERGIVLTPDILTNAGGVTVSYFEWV
HHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHCCCEECCHHHCCCCCCCHHHHHHH
QNLYGYYWSEEEVEQKEEIAMVKAFESIWKIKEEYNVTMREAAYMHSIKKVAEAMKLRGW
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCC
Y
C
>Mature Secondary Structure 
SGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSIPVKMDDGSIKTFKGFR
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHH
SQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIPYGGGKGGIIVDPSTLSQG
HCCCCCCCCCCCCEEECCCCCHHHHEEEEEEEEEEEEEEECCCCCCCCCEEECCCCCCHH
ELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVDEYNKLTGQSSIGVITGKPVE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHH
FGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQGIGNVGSYTVLNCEKLGGTVVA
CCCCCCCHHHCCCCEEEEHHHHHHHHCCCHHHHEEEEEECCCCCCEEEEEHHHHCCHHHH
MAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLNFPGAKRISLEEFWASDVDIVIPAA
HHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCEECCCCCCCCCHHHHCCCCCCEEEEHH
LENSITKEVAESIKAKLVCEAANGPTTPEADEVFAERGIVLTPDILTNAGGVTVSYFEWV
HHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHCCCEECCHHHCCCCCCCHHHHHHH
QNLYGYYWSEEEVEQKEEIAMVKAFESIWKIKEEYNVTMREAAYMHSIKKVAEAMKLRGW
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1774279