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The map label for this gene is nasD [H]

Identifier: 126697749

GI number: 126697749

Start: 233014

End: 234240

Strand: Direct

Name: nasD [H]

Synonym: CD0176

Alternate gene names: 126697749

Gene position: 233014-234240 (Clockwise)

Preceding gene: 126697748

Following gene: 126697751

Centisome position: 5.43

GC content: 28.04

Gene sequence:

>1227_bases
ATGAATTATGTAGTGTTAGGGGCTAGTGCAGCTGGTATAAATGCAGTAAAAACATTAAGAGAATTAGATAAAGATTCAAA
TATAGTAGTAATTTCTAAAGATGAAAACGTATATTCAAGATGTATGTTGCATCATGTTATATCTGAACACAGAACTTTAA
AACAAATAAATTTTGTTGATGAAGATTTTATGGAGCAAAATAATGTAAAATGGATTGCAGGTAAGACTGTTAAAGGTATA
GACATAAATAAAAAGGTAGTTCAGACAGAAGACATTACTGTAAATTATGATAAACTTTTAATAGCTACAGGTGCATCTTC
TGCTATTCCTCCTATAAAAAATTTAAGAGATGGAAATTTTGTATATTCTGTGAGAAACATAGATGATATTTATAAAATAA
AAGAAAAAGCACAAAATTCTAAAAATGTAGTTATAATAGGTGCTGGTTTAGTTGGTATAGATGCATTAGTGGGATTATTT
AAATATGAGCAGTTAAATATTTCTGTAGCTTTTATGGAAAAGTACATTTTAGATAGACAATTGGATGAATATACAGCATC
AGTGTATGAAAATAAGTTCAAAGAAAAAGGTGTAAAATTTTACCCAAGTGCATCAATACAAGAAATTGTATTGGATAACA
GTAAAAATGTTACAGGTGTTGCATTTTCAAATGGTGAAGTTTTAGATGCAGACATGGTAATTGTAGCTACAGGGGTTAAA
CCAAATGCTGACTTTTTAGATGGAACAGGAATAGAATATGATAGAGGTATAATAATAGATGATATGTGCCAAACAACACA
AAAAGACATATATGCAGCAGGAGATGTTGTAGGTAAAAATGCTATTTGGCCTCTGGCAGTAAAGCAAGGAATTGTAGCTG
CCTATAATATGGTTGGAAAAGATAAGAAGATAGAAGATGAATTTGCTTTTAAAAATAGTATGAATTTCATGGATATACCA
ACTATTTCTATAGGAATGAATACACCTGTAGATGATAGCTATAAAGTATTAACCAGACATGGAATTAATGATTATAAAAA
GTTTGTTTTTAAAGATAATGTAATCTATGGAGCAGTTATACAAGGAGATATTTCATATGTAGGTGTATTAACTTATTTAA
TAAAAAATAAAGTTGAAATATATGATTTAGAAAATAGGATATTTGATATATGTTATGCAGACTTTTTTAATATAAAAGAA
AATGGAGAATTTTGTTATAGTGTATAG

Upstream 100 bases:

>100_bases
CAGAACATACTTAAATGTGATTTATGTAAAGATGAGGAGTATCCTAGATGTGTAGCTAATTGCCCAAGTGGAGCAATTGA
ATTACAGAAGGAGGAAAATG

Downstream 100 bases:

>100_bases
TATATACGTGGAGTAAGGTTTATAAATACAGTAAATCAATAAATAGAGTTATTCTAAAATAAGAAAAATGACAAATCTTA
TTTAGAAATAGCTCTATTTT

Product: oxidoreductase, NAD/FAD binding subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLHHVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGI
DINKKVVQTEDITVNYDKLLIATGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVTGVAFSNGEVLDADMVIVATGVK
PNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDVVGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIP
TISIGMNTPVDDSYKVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICYADFFNIKE
NGEFCYSV

Sequences:

>Translated_408_residues
MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLHHVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGI
DINKKVVQTEDITVNYDKLLIATGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVTGVAFSNGEVLDADMVIVATGVK
PNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDVVGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIP
TISIGMNTPVDDSYKVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICYADFFNIKE
NGEFCYSV
>Mature_408_residues
MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLHHVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGI
DINKKVVQTEDITVNYDKLLIATGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVTGVAFSNGEVLDADMVIVATGVK
PNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDVVGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIP
TISIGMNTPVDDSYKVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICYADFFNIKE
NGEFCYSV

Specific function: Required for nitrite assimilation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=318, Percent_Identity=28.3018867924528, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI21389617, Length=318, Percent_Identity=28.3018867924528, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI226437568, Length=318, Percent_Identity=28.3018867924528, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI22202629, Length=275, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4757732, Length=275, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1789765, Length=372, Percent_Identity=27.9569892473118, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789065, Length=319, Percent_Identity=25.3918495297806, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1788892, Length=278, Percent_Identity=24.8201438848921, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17559934, Length=328, Percent_Identity=27.7439024390244, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24639257, Length=318, Percent_Identity=25.4716981132075, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281359715, Length=318, Percent_Identity=25.4716981132075, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI281359713, Length=318, Percent_Identity=25.4716981132075, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24639250, Length=318, Percent_Identity=25.4716981132075, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI18543267, Length=318, Percent_Identity=25.4716981132075, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24639252, Length=318, Percent_Identity=25.4716981132075, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24581020, Length=358, Percent_Identity=24.8603351955307, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28573993, Length=358, Percent_Identity=24.8603351955307, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585130, Length=319, Percent_Identity=23.5109717868339, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 45691; Mature: 45691

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLHHVISEHRTLKQINFVD
CCEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
EDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIATGASSAIPPIKNLRDGNF
HHHHCCCCEEEEECCEEECCCCCCEEEEECCEEEECCEEEEEECCCCCCCCHHCCCCCCE
VYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLFKYEQLNISVAFMEKYILDRQ
EEEECCHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHH
LDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVTGVAFSNGEVLDADMVIVATGVK
HHHHHHHHHHHHHHHCCCEECCCCCHHHHEECCCCCEEEEEECCCCEEECCEEEEEECCC
PNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDVVGKNAIWPLAVKQGIVAAYNMVGK
CCCCCCCCCCCCCCCCEEEHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCC
DKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSYKVLTRHGINDYKKFVFKDNVIYGAVI
CCCCCHHHHHHCCCCEEECCEEEECCCCCCCCCHHHHHHCCCCHHHHHEEECCEEEEEEE
QGDISYVGVLTYLIKNKVEIYDLENRIFDICYADFFNIKENGEFCYSV
ECCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEC
>Mature Secondary Structure
MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLHHVISEHRTLKQINFVD
CCEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
EDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIATGASSAIPPIKNLRDGNF
HHHHCCCCEEEEECCEEECCCCCCEEEEECCEEEECCEEEEEECCCCCCCCHHCCCCCCE
VYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLFKYEQLNISVAFMEKYILDRQ
EEEECCHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHH
LDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVTGVAFSNGEVLDADMVIVATGVK
HHHHHHHHHHHHHHHCCCEECCCCCHHHHEECCCCCEEEEEECCCCEEECCEEEEEECCC
PNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDVVGKNAIWPLAVKQGIVAAYNMVGK
CCCCCCCCCCCCCCCCEEEHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCC
DKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSYKVLTRHGINDYKKFVFKDNVIYGAVI
CCCCCHHHHHHCCCCEEECCEEEECCCCCCCCCHHHHHHCCCCHHHHHEEECCEEEEEEE
QGDISYVGVLTYLIKNKVEIYDLENRIFDICYADFFNIKENGEFCYSV
ECCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]