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The map label for this gene is prfB [H]

Identifier: 126697716

GI number: 126697716

Start: 192087

End: 193073

Strand: Direct

Name: prfB [H]

Synonym: CD0144

Alternate gene names: 126697716

Gene position: 192087-193073 (Clockwise)

Preceding gene: 126697715

Following gene: 126697717

Centisome position: 4.48

GC content: 30.6

Gene sequence:

>987_bases
ATGAATCAACAAGACTTTTGGAATGATAATGAAGTCGCTCAAAGAGTACTTCAAGAAAATAAATCTTTAAAAGAGACATT
AGAAGAATATGAAAGCTTAAAAAGCTTATTAGAAGATATAGAAGTATTAATTGAAATAGGTTTAGAAGAAGATGATGATT
CTGTAGAAAGAGATATAGAAAAGTCCATAGAATCAATGGAAGAAAAATTATCTGAAATGAAAATAAAGACTCTTCTAAAT
GGAGAGTATGATAAAAATAATGCTATATTATCTATAAATGCTGGTACTGGTGGCTTAGATGCTCAAGATTGGGCTCAAAT
GCTTTTGAGAATGTATATAAGATGGTCAGAAAGTAAGGGTTATAAAGTTAAGCTTTTAGATATAATATCAGACCCTGAAG
CAGGGATAAAGACAGCTACAATTCTTGTAGAAGGAACAAATGCTTATGGGTATTTAAAGAGTGAAAAAGGAGTTCATAGA
TTAGTGAGAATATCTCCATTTGACCCATCAGGAAAAAGACATACTTCATTTGCATCTATAGATGTAACACCAGAGTTAGA
TGAAAATATAGAAGTAGAAATTAATCCATCAGATTTAAAGATAGACACATATAGAGCTTCTGGAGCTGGAGGGCAACATG
TAAATACTACTGATTCAGCAGTTAGAATAACACATATCCCTACTGGTGTAGTAGTTCAGTGCCAAAATGAAAGGTCTCAG
CATCTAAATAAGGACAGAGCTATGAGACTTTTAATGGCAAAATTGATAGAATTAAAAGAGTTAGAACAAAAGGAAAAAAT
AGAAGATATACAAGGCAAATACTCACAAATAACTTGGGGTAGTCAAATTAGGTCATATGTTTTTCAACCATATAAATTGG
TTAAAGACCATAGAACAAATGCTGAGTTTGGAAATGTAGATAGTGTAATGAATGGAAATATAGACTTGTTTATTAATGAA
TACTTAAAAATGAATAAAATAGTTTAA

Upstream 100 bases:

>100_bases
AAACATAGTATGGAGCAGATGGCTTCAAATTTAGAAGAATTGAGGGTTTCTCTTTGACATAGCTTCATTGATGAAAAATA
TACAAATTAATGAAGAAAAG

Downstream 100 bases:

>100_bases
TAAATATGCACTGTAATTAAGATATATGTATAAGAAGGTTAGTGCTTAATATGCACTTACAGCACTTAGGCTGAGGACGA
TAACCCTCAGCCTTCGTTCT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MNQQDFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKSIESMEEKLSEMKIKTLLN
GEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKGYKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHR
LVRISPFDPSGKRHTSFASIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQNERSQ
HLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLVKDHRTNAEFGNVDSVMNGNIDLFINE
YLKMNKIV

Sequences:

>Translated_328_residues
MNQQDFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKSIESMEEKLSEMKIKTLLN
GEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKGYKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHR
LVRISPFDPSGKRHTSFASIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQNERSQ
HLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLVKDHRTNAEFGNVDSVMNGNIDLFINE
YLKMNKIV
>Mature_328_residues
MNQQDFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKSIESMEEKLSEMKIKTLLN
GEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKGYKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHR
LVRISPFDPSGKRHTSFASIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQNERSQ
HLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLVKDHRTNAEFGNVDSVMNGNIDLFINE
YLKMNKIV

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=284, Percent_Identity=36.2676056338028, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI34577120, Length=321, Percent_Identity=36.1370716510903, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI166795305, Length=185, Percent_Identity=36.7567567567568, Blast_Score=128, Evalue=6e-30,
Organism=Escherichia coli, GI2367172, Length=323, Percent_Identity=48.9164086687306, Blast_Score=333, Evalue=7e-93,
Organism=Escherichia coli, GI1787462, Length=238, Percent_Identity=42.436974789916, Blast_Score=194, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=284, Percent_Identity=33.8028169014084, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321295, Length=305, Percent_Identity=33.4426229508197, Blast_Score=146, Evalue=4e-36,
Organism=Drosophila melanogaster, GI19921226, Length=231, Percent_Identity=39.8268398268398, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37404; Mature: 37404

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQQDFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIE
CCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KSIESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFASI
CEEEEEEEECCCCCCCEEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEEE
DVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQNERSQ
ECCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHH
HLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLVKDHRTN
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHCHHHHHHHCCCC
AEFGNVDSVMNGNIDLFINEYLKMNKIV
CCCCCCHHHHCCCEEEEEHHHHHHHCCC
>Mature Secondary Structure
MNQQDFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIE
CCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KSIESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFASI
CEEEEEEEECCCCCCCEEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEEE
DVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQNERSQ
ECCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHH
HLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLVKDHRTN
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHCHHHHHHHCCCC
AEFGNVDSVMNGNIDLFINEYLKMNKIV
CCCCCCHHHHCCCEEEEEHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA