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The map label for this gene is lpfB [H]

Identifier: 126454040

GI number: 126454040

Start: 2084732

End: 2085502

Strand: Reverse

Name: lpfB [H]

Synonym: BURPS1106A_2089

Alternate gene names: 126454040

Gene position: 2085502-2084732 (Counterclockwise)

Preceding gene: 126453583

Following gene: 126454737

Centisome position: 52.29

GC content: 64.59

Gene sequence:

>771_bases
ATGCGCCTGCCTACCGTTTGTCATGCCGCTTTTACCGCCGTCCTCGCCCTGTGCGGCACCGCAGCCCACGCGGGTATCCA
GGTAGGCGGCACACGCGTGGTTTTCGACGCGAAAGTCGATGTGCGCGATGCATCGGTTGCCGTGCGCAACAAGGGCGAGA
CGCCGTACGTGATCCAGATTTTCGTCGACGACGGCAACGGCAATACCCGCCGCATGCCGTTTGCAGTGACGCCGCCGCTT
TTTCGGCTGGACGGCGGCAAGGAACAGCGCGTCAGCGTGCGTTACGTGAAGCGAGGCGCGGGGTTGCCGCAGGACGTCGA
ATCCGTTTATTGGCTCAACGTCAAGGAGATTCCGCCCACCGAGAAGCGCAACGCGGACATCAACACGCTCAAGATCGCCG
TCCTCACGCGCATCAAGCTCTTTTACCGGCCGGCGGGCCTCGCCGGCAGCGCGGCCGACGCGCCGTCGCGGCTCAAATGG
TCCGTCGTGCCGAACCCGATCGGGCAAGGCGTGGCGCTGAAGGTGAGCAACCCGAGCGCGTACCACGTGACCTTTTCGAC
GATCGAGATTCAGGCCGCGCAGAACGCGAGCGTCAACGCCGACATGGTGAATCCGAAAGGCGAACTGATCGTTCCCATTC
CGCCGAAGGCCGTCTCGCAGGTCGGCCCCGTCAAATTCAGCTATACCACCATCAACGATTACGGCGCCGTCACGCCGGCG
ACGGAAGTGACGGCCCAACCGGCCGGTACGCCCGAGGCCGCGCCGCAGTAG

Upstream 100 bases:

>100_bases
AGCGGGCGCCGAAGCGTTCCTGCATGCGTGCAAGTGAGGATGCAAAACCGGATTCCTTGACGCGGCCCCGAGCCGCGGCT
TTCCCAGAGAGAGACGAAAC

Downstream 100 bases:

>100_bases
GACGCGATGATTCGGCATCGTGTCGCCGATGTCGGCTTCTCGCGCATACGAGTGACCATGCTGGCCGCCGCGCTCACGGC
GCTTTCGGCGACCGCTCGCG

Product: fimbrial assembly chaperone protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRLPTVCHAAFTAVLALCGTAAHAGIQVGGTRVVFDAKVDVRDASVAVRNKGETPYVIQIFVDDGNGNTRRMPFAVTPPL
FRLDGGKEQRVSVRYVKRGAGLPQDVESVYWLNVKEIPPTEKRNADINTLKIAVLTRIKLFYRPAGLAGSAADAPSRLKW
SVVPNPIGQGVALKVSNPSAYHVTFSTIEIQAAQNASVNADMVNPKGELIVPIPPKAVSQVGPVKFSYTTINDYGAVTPA
TEVTAQPAGTPEAAPQ

Sequences:

>Translated_256_residues
MRLPTVCHAAFTAVLALCGTAAHAGIQVGGTRVVFDAKVDVRDASVAVRNKGETPYVIQIFVDDGNGNTRRMPFAVTPPL
FRLDGGKEQRVSVRYVKRGAGLPQDVESVYWLNVKEIPPTEKRNADINTLKIAVLTRIKLFYRPAGLAGSAADAPSRLKW
SVVPNPIGQGVALKVSNPSAYHVTFSTIEIQAAQNASVNADMVNPKGELIVPIPPKAVSQVGPVKFSYTTINDYGAVTPA
TEVTAQPAGTPEAAPQ
>Mature_256_residues
MRLPTVCHAAFTAVLALCGTAAHAGIQVGGTRVVFDAKVDVRDASVAVRNKGETPYVIQIFVDDGNGNTRRMPFAVTPPL
FRLDGGKEQRVSVRYVKRGAGLPQDVESVYWLNVKEIPPTEKRNADINTLKIAVLTRIKLFYRPAGLAGSAADAPSRLKW
SVVPNPIGQGVALKVSNPSAYHVTFSTIEIQAAQNASVNADMVNPKGELIVPIPPKAVSQVGPVKFSYTTINDYGAVTPA
TEVTAQPAGTPEAAPQ

Specific function: Required for the biogenesis of long polar fimbria; binds and interact with lpfA [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1790771, Length=219, Percent_Identity=38.3561643835616, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786333, Length=234, Percent_Identity=32.9059829059829, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789532, Length=228, Percent_Identity=34.6491228070175, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1787171, Length=238, Percent_Identity=33.6134453781513, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1786743, Length=233, Percent_Identity=32.618025751073, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1788428, Length=220, Percent_Identity=31.8181818181818, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788677, Length=212, Percent_Identity=33.0188679245283, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786936, Length=176, Percent_Identity=28.4090909090909, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI87082203, Length=229, Percent_Identity=24.4541484716157, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081806, Length=239, Percent_Identity=26.7782426778243, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 27240; Mature: 27240

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPTVCHAAFTAVLALCGTAAHAGIQVGGTRVVFDAKVDVRDASVAVRNKGETPYVIQI
CCCCHHHHHHHHHHHHHHCCHHHCCEEECCEEEEEEEEEECCCCEEEEECCCCCCEEEEE
FVDDGNGNTRRMPFAVTPPLFRLDGGKEQRVSVRYVKRGAGLPQDVESVYWLNVKEIPPT
EEECCCCCCEECCEEECCCEEEECCCCCCEEEEEEEECCCCCCCCCCEEEEEEEECCCCC
EKRNADINTLKIAVLTRIKLFYRPAGLAGSAADAPSRLKWSVVPNPIGQGVALKVSNPSA
CCCCCCCCEEEEEEHHEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCE
YHVTFSTIEIQAAQNASVNADMVNPKGELIVPIPPKAVSQVGPVKFSYTTINDYGAVTPA
EEEEEEEEEEEECCCCCCCEEEECCCCCEEEECCCHHHHHCCCEEEEEEEECCCCCCCCC
TEVTAQPAGTPEAAPQ
HHEEECCCCCCCCCCC
>Mature Secondary Structure
MRLPTVCHAAFTAVLALCGTAAHAGIQVGGTRVVFDAKVDVRDASVAVRNKGETPYVIQI
CCCCHHHHHHHHHHHHHHCCHHHCCEEECCEEEEEEEEEECCCCEEEEECCCCCCEEEEE
FVDDGNGNTRRMPFAVTPPLFRLDGGKEQRVSVRYVKRGAGLPQDVESVYWLNVKEIPPT
EEECCCCCCEECCEEECCCEEEECCCCCCEEEEEEEECCCCCCCCCCEEEEEEEECCCCC
EKRNADINTLKIAVLTRIKLFYRPAGLAGSAADAPSRLKWSVVPNPIGQGVALKVSNPSA
CCCCCCCCEEEEEEHHEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCE
YHVTFSTIEIQAAQNASVNADMVNPKGELIVPIPPKAVSQVGPVKFSYTTINDYGAVTPA
EEEEEEEEEEEECCCCCCCEEEECCCCCEEEECCCHHHHHCCCEEEEEEEECCCCCCCCC
TEVTAQPAGTPEAAPQ
HHEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7721701; 11677609 [H]