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The map label for this gene is def [H]

Identifier: 126453149

GI number: 126453149

Start: 151300

End: 151839

Strand: Direct

Name: def [H]

Synonym: BURPS1106A_0159

Alternate gene names: 126453149

Gene position: 151300-151839 (Clockwise)

Preceding gene: 126451470

Following gene: 126454119

Centisome position: 3.79

GC content: 61.48

Gene sequence:

>540_bases
ATGCGAGCGGGTCGTTCGTCTCCCGACACTGAAATCATGGCTTTGCTGAACATTCTTCACTACCCCGATAAACGGCTGCA
CAAGGTGGCCAAGCCGGTCGCCAAAGTGGACGACCGCATTCGCAAGCTCGTCGCCGACATGGCCGAGACGATGTACGCGG
CGCCCGGCATCGGCCTTGCTGCGACGCAGGTCGACGTGCATGAACGCGTGATCGTGATCGACGTGTCCGAGGACAAGAAC
GAGCTGCGCGTCTTCATCAACCCGGAAATCGTCTGGACGGGCGACGGCAAGCAGGTCTATGAGGAAGGCTGCCTGTCGGT
GCCGGGTGTCTATGACGAAGTGGAGCGCCCGGACCGGGTGCGCGTGCGCGCGCTCGACGGCCAAGGCGAGCCGTTCGAGC
TCGATTGCGAAGGGCTGCTCGCGGTGTGCATCCAGCACGAGATGGATCACCTGATGGGTCGCGTGTTCGTCCAGTATCTG
TCGCCGCTCAAGCAGACCCGGATCAAGACGAAGATGAAGAAACTCGAACGCGCAATGTGA

Upstream 100 bases:

>100_bases
CCCACGGCGATTGGCCGATCATTGCGACGCGCGGATGGCGCGCGTCGTCGAGTCCATCTTGAGGCCGGCGCGCGCATCGC
GGCAGTCGGCCGAACGGCCG

Downstream 100 bases:

>100_bases
TGCGCGCCGCCGCTACCCGATTCATTCGCACATGACACATTCACTGCGCGTCATTTTTGCCGGCACGCCGGAATTCGCGG
CGGCGGCGCTCGCCGCGATC

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MRAGRSSPDTEIMALLNILHYPDKRLHKVAKPVAKVDDRIRKLVADMAETMYAAPGIGLAATQVDVHERVIVIDVSEDKN
ELRVFINPEIVWTGDGKQVYEEGCLSVPGVYDEVERPDRVRVRALDGQGEPFELDCEGLLAVCIQHEMDHLMGRVFVQYL
SPLKQTRIKTKMKKLERAM

Sequences:

>Translated_179_residues
MRAGRSSPDTEIMALLNILHYPDKRLHKVAKPVAKVDDRIRKLVADMAETMYAAPGIGLAATQVDVHERVIVIDVSEDKN
ELRVFINPEIVWTGDGKQVYEEGCLSVPGVYDEVERPDRVRVRALDGQGEPFELDCEGLLAVCIQHEMDHLMGRVFVQYL
SPLKQTRIKTKMKKLERAM
>Mature_179_residues
MRAGRSSPDTEIMALLNILHYPDKRLHKVAKPVAKVDDRIRKLVADMAETMYAAPGIGLAATQVDVHERVIVIDVSEDKN
ELRVFINPEIVWTGDGKQVYEEGCLSVPGVYDEVERPDRVRVRALDGQGEPFELDCEGLLAVCIQHEMDHLMGRVFVQYL
SPLKQTRIKTKMKKLERAM

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=154, Percent_Identity=29.8701298701299, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1789682, Length=165, Percent_Identity=58.7878787878788, Blast_Score=201, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24645728, Length=143, Percent_Identity=34.965034965035, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24645726, Length=137, Percent_Identity=32.1167883211679, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20283; Mature: 20283

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAGRSSPDTEIMALLNILHYPDKRLHKVAKPVAKVDDRIRKLVADMAETMYAAPGIGLA
CCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
ATQVDVHERVIVIDVSEDKNELRVFINPEIVWTGDGKQVYEEGCLSVPGVYDEVERPDRV
EEEEECCCEEEEEEECCCCCCEEEEECCEEEEECCCHHHHHHCCCCCCCCHHHHCCCCCE
RVRALDGQGEPFELDCEGLLAVCIQHEMDHLMGRVFVQYLSPLKQTRIKTKMKKLERAM
EEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRAGRSSPDTEIMALLNILHYPDKRLHKVAKPVAKVDDRIRKLVADMAETMYAAPGIGLA
CCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
ATQVDVHERVIVIDVSEDKNELRVFINPEIVWTGDGKQVYEEGCLSVPGVYDEVERPDRV
EEEEECCCEEEEEEECCCCCCEEEEECCEEEEECCCHHHHHHCCCCCCCCHHHHCCCCCE
RVRALDGQGEPFELDCEGLLAVCIQHEMDHLMGRVFVQYLSPLKQTRIKTKMKKLERAM
EEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]