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The map label for this gene is cysI

Identifier: 126209304

GI number: 126209304

Start: 2071381

End: 2073147

Strand: Reverse

Name: cysI

Synonym: APL_1842

Alternate gene names: 126209304

Gene position: 2073147-2071381 (Counterclockwise)

Preceding gene: 126209305

Following gene: 126209299

Centisome position: 91.15

GC content: 41.31

Gene sequence:

>1767_bases
ATGAGTGATAAAAAAATCAAAGGCTTAGAGTGGCAAGAAAAACCGTTATCTGACAACGAGCGTTTAAAAACTGATAGTAA
TTTCTTACGTGGCACAATTTTAGATGATTTAAAAGACGACTTAACCGGCGGTTTCAAAGGCGATAATTTCCAACTGATTC
GCTTTCACGGTATGTATGAACAAGATGACCGTGATATTCGTGCCGAGCGTTTAGAAGAAAAACTCGAACCGTTAAAATTT
ATGTTACTTCGCTGCCGTTTGCCCGGTGGTATTATCAAACCTTATCAATGGATTGAGATTGATAAATTTGCCCGTGAGCA
TACCCGTTATCAGTCGATTCGTTTAACTAATCGCCAAACTTTCCAATATCACGGCGTACCAAAAGGCAAATTACAACCAA
TGCACCGTTTGTTACATAGTATCGGTCTAGATTCAATTGCAACCGCTGCCGATATGAATCGTAATGTACTTTGTACTTCC
AATCCGATTGAAAGCGAATTACATCAACAAGCTTACGAGTTTGCGAAAAAGATTTCGGAACATCTCTTGCCTCGTTCTCG
TGGTTATTTAGATGTTTGGGTCGATGGTAAAAAAGTCGAAAGCTCGGACGATTTACTTAAAATTGAAGATGAACCGATTC
TCGGTAAAACTTATCTTCCTCGAAAATTTAAAACCGCCGTTGCTATTCCACCGCTTAACGATGTTGATGTGTATGCCAAC
GATTTGAACTTTATTGCGATTCAAGATGAAAACGGACAGCTATGCGGCTTTAACGTATTAGTTGGTGGTGGGTTATCCTT
TGAACACGGTAATACCAAAACCTACCCGAATGTAGCCTATTCACTCGGCTTTGTACCATTGGAACATACGCTTGCCGCTG
CCGAAGGCGTAGTAAAAACTCAACGCGATTTCGGTAACCGTTCCGACCGTAAAAATGCACGTGTACGTTATACCGTACAA
AATATGACGCTTGACGGTTTTAGAGCGGAAGTAGAACGTTGTATGAATATTAAATTCGAACCGACACGTCCGTATGAATT
TACTGAACGTGGAGACCGTATCGGCTGGGTTAAAGGTATCGATAATAACTGGCACTTAACCTTATTCATTGAAAGCGTTC
GTATTACCGATAAACCTGAAAAACCTTTAATGACGGGCGTGCTGGAACTCGCTAAAGTACACAAAGGTGACTTCCGTATT
ACCGCAAACCAAAATTTAATCGTTGCTAATGTTGCCGAACAAGATAAAGCGCAAATTGAAGCAATTGCTCGCCAATACGG
ATTAATCCAAGAAATTTCAAAATTGCGTGAAAACGCAATGTCTTGTGTTTCCTTACCGACTTGTCCACTAGCAATGGCGG
AAGCGGAACGCGTATTACCGGATTTTATTAGCGAACTCGATAAAGTCTTGAGTAAGCACAATGTTGCAGACGAAAGCATT
ATTACCCGTATCACGGGCTGTCCAAATGGTTGCGGACGGGCAATGTTAGCGGAAATCGGCTTAGTCGGTAAAGCGATTGG
ACGCTATAACCTACATATCGGCGGCGATCGTGCCGGTTTGCGTATTTCTCGCTTATATAAAGAAAATATTACCTTACAGG
AAATCGTCAATGAAATTGACCAATTAGTTGCACGCTGGGCAACGGAACGTCAAACCAACGAAGCTTTCGGCGATTTTGTG
ATTCGCTCTAATATCATTGCCCCGGTTGTCAATGCTCATATTGATTTCTGGGATGCAACGAAAATTATTCCAACAACTAT
AATCTAA

Upstream 100 bases:

>100_bases
GAATGCGGATGAAGCGGAAGAATACTTAGATAACTTACGTGAAGAAAAACGTTATCAACGTGATGTTTATTAAAAATTAG
TGGAATTTAGGAGTGAGAAA

Downstream 100 bases:

>100_bases
ACCTCGTGGTATAACACACCACCTTGCGGTTAATCTGTAAAGTAGGGCAGATTGACCGCTTTTTTTATGCCGTACGATAT
CGAATTAATTCAGCTCATTT

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MSDKKIKGLEWQEKPLSDNERLKTDSNFLRGTILDDLKDDLTGGFKGDNFQLIRFHGMYEQDDRDIRAERLEEKLEPLKF
MLLRCRLPGGIIKPYQWIEIDKFAREHTRYQSIRLTNRQTFQYHGVPKGKLQPMHRLLHSIGLDSIATAADMNRNVLCTS
NPIESELHQQAYEFAKKISEHLLPRSRGYLDVWVDGKKVESSDDLLKIEDEPILGKTYLPRKFKTAVAIPPLNDVDVYAN
DLNFIAIQDENGQLCGFNVLVGGGLSFEHGNTKTYPNVAYSLGFVPLEHTLAAAEGVVKTQRDFGNRSDRKNARVRYTVQ
NMTLDGFRAEVERCMNIKFEPTRPYEFTERGDRIGWVKGIDNNWHLTLFIESVRITDKPEKPLMTGVLELAKVHKGDFRI
TANQNLIVANVAEQDKAQIEAIARQYGLIQEISKLRENAMSCVSLPTCPLAMAEAERVLPDFISELDKVLSKHNVADESI
ITRITGCPNGCGRAMLAEIGLVGKAIGRYNLHIGGDRAGLRISRLYKENITLQEIVNEIDQLVARWATERQTNEAFGDFV
IRSNIIAPVVNAHIDFWDATKIIPTTII

Sequences:

>Translated_588_residues
MSDKKIKGLEWQEKPLSDNERLKTDSNFLRGTILDDLKDDLTGGFKGDNFQLIRFHGMYEQDDRDIRAERLEEKLEPLKF
MLLRCRLPGGIIKPYQWIEIDKFAREHTRYQSIRLTNRQTFQYHGVPKGKLQPMHRLLHSIGLDSIATAADMNRNVLCTS
NPIESELHQQAYEFAKKISEHLLPRSRGYLDVWVDGKKVESSDDLLKIEDEPILGKTYLPRKFKTAVAIPPLNDVDVYAN
DLNFIAIQDENGQLCGFNVLVGGGLSFEHGNTKTYPNVAYSLGFVPLEHTLAAAEGVVKTQRDFGNRSDRKNARVRYTVQ
NMTLDGFRAEVERCMNIKFEPTRPYEFTERGDRIGWVKGIDNNWHLTLFIESVRITDKPEKPLMTGVLELAKVHKGDFRI
TANQNLIVANVAEQDKAQIEAIARQYGLIQEISKLRENAMSCVSLPTCPLAMAEAERVLPDFISELDKVLSKHNVADESI
ITRITGCPNGCGRAMLAEIGLVGKAIGRYNLHIGGDRAGLRISRLYKENITLQEIVNEIDQLVARWATERQTNEAFGDFV
IRSNIIAPVVNAHIDFWDATKIIPTTII
>Mature_587_residues
SDKKIKGLEWQEKPLSDNERLKTDSNFLRGTILDDLKDDLTGGFKGDNFQLIRFHGMYEQDDRDIRAERLEEKLEPLKFM
LLRCRLPGGIIKPYQWIEIDKFAREHTRYQSIRLTNRQTFQYHGVPKGKLQPMHRLLHSIGLDSIATAADMNRNVLCTSN
PIESELHQQAYEFAKKISEHLLPRSRGYLDVWVDGKKVESSDDLLKIEDEPILGKTYLPRKFKTAVAIPPLNDVDVYAND
LNFIAIQDENGQLCGFNVLVGGGLSFEHGNTKTYPNVAYSLGFVPLEHTLAAAEGVVKTQRDFGNRSDRKNARVRYTVQN
MTLDGFRAEVERCMNIKFEPTRPYEFTERGDRIGWVKGIDNNWHLTLFIESVRITDKPEKPLMTGVLELAKVHKGDFRIT
ANQNLIVANVAEQDKAQIEAIARQYGLIQEISKLRENAMSCVSLPTCPLAMAEAERVLPDFISELDKVLSKHNVADESII
TRITGCPNGCGRAMLAEIGLVGKAIGRYNLHIGGDRAGLRISRLYKENITLQEIVNEIDQLVARWATERQTNEAFGDFVI
RSNIIAPVVNAHIDFWDATKIIPTTII

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=566, Percent_Identity=66.2544169611307, Blast_Score=811, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322597, Length=589, Percent_Identity=43.4634974533107, Blast_Score=494, Evalue=1e-140,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSI_ACTP2 (A3N3D8)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054529.1
- ProteinModelPortal:   A3N3D8
- SMR:   A3N3D8
- STRING:   A3N3D8
- GeneID:   4848697
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1842
- eggNOG:   COG0155
- HOGENOM:   HBG626671
- OMA:   VGDEHIV
- PhylomeDB:   A3N3D8
- ProtClustDB:   PRK13504
- BioCyc:   APLE416269:APL_1842-MONOMER
- HAMAP:   MF_01540
- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397
- TIGRFAMs:   TIGR02041

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.1.2

Molecular weight: Translated: 66762; Mature: 66631

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKKIKGLEWQEKPLSDNERLKTDSNFLRGTILDDLKDDLTGGFKGDNFQLIRFHGMYE
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
QDDRDIRAERLEEKLEPLKFMLLRCRLPGGIIKPYQWIEIDKFAREHTRYQSIRLTNRQT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHEEEECCCCE
FQYHGVPKGKLQPMHRLLHSIGLDSIATAADMNRNVLCTSNPIESELHQQAYEFAKKISE
EEECCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHH
HLLPRSRGYLDVWVDGKKVESSDDLLKIEDEPILGKTYLPRKFKTAVAIPPLNDVDVYAN
HCCCCCCCEEEEEECCCEECCCCCEEEECCCCCCCCCCCCCHHCCEEECCCCCCCCEEEC
DLNFIAIQDENGQLCGFNVLVGGGLSFEHGNTKTYPNVAYSLGFVPLEHTLAAAEGVVKT
CEEEEEEECCCCCEEEEEEEEECCEEECCCCCCCCCCHHHEECCCCHHHHHHHHHHHHHH
QRDFGNRSDRKNARVRYTVQNMTLDGFRAEVERCMNIKFEPTRPYEFTERGDRIGWVKGI
HHHCCCCCCCCCCEEEEEEECCEECHHHHHHHHHHCCCCCCCCCCCHHCCCCEEEEEEEC
DNNWHLTLFIESVRITDKPEKPLMTGVLELAKVHKGDFRITANQNLIVANVAEQDKAQIE
CCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEECCHHHHHHHH
AIARQYGLIQEISKLRENAMSCVSLPTCPLAMAEAERVLPDFISELDKVLSKHNVADESI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
ITRITGCPNGCGRAMLAEIGLVGKAIGRYNLHIGGDRAGLRISRLYKENITLQEIVNEID
HHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHH
QLVARWATERQTNEAFGDFVIRSNIIAPVVNAHIDFWDATKIIPTTII
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEECCCCCCCCCCC
>Mature Secondary Structure 
SDKKIKGLEWQEKPLSDNERLKTDSNFLRGTILDDLKDDLTGGFKGDNFQLIRFHGMYE
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
QDDRDIRAERLEEKLEPLKFMLLRCRLPGGIIKPYQWIEIDKFAREHTRYQSIRLTNRQT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHEEEECCCCE
FQYHGVPKGKLQPMHRLLHSIGLDSIATAADMNRNVLCTSNPIESELHQQAYEFAKKISE
EEECCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHH
HLLPRSRGYLDVWVDGKKVESSDDLLKIEDEPILGKTYLPRKFKTAVAIPPLNDVDVYAN
HCCCCCCCEEEEEECCCEECCCCCEEEECCCCCCCCCCCCCHHCCEEECCCCCCCCEEEC
DLNFIAIQDENGQLCGFNVLVGGGLSFEHGNTKTYPNVAYSLGFVPLEHTLAAAEGVVKT
CEEEEEEECCCCCEEEEEEEEECCEEECCCCCCCCCCHHHEECCCCHHHHHHHHHHHHHH
QRDFGNRSDRKNARVRYTVQNMTLDGFRAEVERCMNIKFEPTRPYEFTERGDRIGWVKGI
HHHCCCCCCCCCCEEEEEEECCEECHHHHHHHHHHCCCCCCCCCCCHHCCCCEEEEEEEC
DNNWHLTLFIESVRITDKPEKPLMTGVLELAKVHKGDFRITANQNLIVANVAEQDKAQIE
CCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEECCHHHHHHHH
AIARQYGLIQEISKLRENAMSCVSLPTCPLAMAEAERVLPDFISELDKVLSKHNVADESI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
ITRITGCPNGCGRAMLAEIGLVGKAIGRYNLHIGGDRAGLRISRLYKENITLQEIVNEID
HHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHH
QLVARWATERQTNEAFGDFVIRSNIIAPVVNAHIDFWDATKIIPTTII
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA