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The map label for this gene is cysJ [H]

Identifier: 126209305

GI number: 126209305

Start: 2073175

End: 2074986

Strand: Reverse

Name: cysJ [H]

Synonym: APL_1843

Alternate gene names: 126209305

Gene position: 2074986-2073175 (Counterclockwise)

Preceding gene: 126209306

Following gene: 126209304

Centisome position: 91.23

GC content: 43.76

Gene sequence:

>1812_bases
ATGAGCCTTAAGACTACAGGCGAATTACCTTTATCCGCTGAAACCGTACAAGTTTTGGCAAATTTAGATCATTTGCAACT
GGCATGGCTGTCCGGTTATGCGTGGGCAAAAGCACAAAGCACAAATAGTATTGGTGAAAATTTTGCAAAAGATTCGTCAA
ATTTGACCGCTTTGGTGGCATCAGAACCGCTTAAAGTAACGGTGTTGTCCGCTTCACAAACCGGTAATGCGAAATCCGTT
GCCGATAAACTCGCCGAACGCTTAACCGCTGAAGGGGTAAATGTGGTTCGCACCTCATTAAAAGACTATAAAGCGAAAAA
TATTGCCGATGAACAGCTCGTCTTATTAGTCACCTCAACTCAGGGCGAAGGCGAAGCGCCGGAAGAAGGTGTGGTGTTGT
TAAAGCTACTCAATGGTAAAAAAGCGCCGAAATTAGACCGCTTACAATTTGCCGTATTAGGTTTAGGCGATTCTTCTTAT
CCGAACTTCTGCCAAGCTGGTAAAGATTTTGATCAGCGTTTTTATGATTTAGGTGTAACGCGTTTATTCGAACGTGTCGA
TGCAGATTTAGACTTCCAAGCAACCGCCGATCAATGGATCAATGAGATTGTCGAGATCATTAAAGCCAAAAATAACGAAG
GTGTAGCAACTAATTTAACGCCAAATGCGTCCCAAACTCCCGTAGTAACCAATCAATCCAAATACAATAAAGCGAATCCG
TTTCCGGCAACGCTTATTACCAACCAACGTATCACCGCAAAAGATGCGGAAAAAGATGTACGTCATTTAGAATTTGATTT
AAGCGGTTCCGGGTTAAGTTACCAATCCGGCGATGTTCTGGGTGTTTACTTTGAAAATGACCCGGCGTTAGCCAATGAAA
TCCTAACCGCCCTCGGCTTATCCGCAGAAGAACAAGTGACTCTGCAAGATCAAACGCTACCGTTATCTACTGCTTTACAA
ACGCAATTTGAATTAACGCAAAACACAGCGGCTTTCGTCAAGAATTATGCAGCGCTCGCTAATCATGCAGAACTCAATGC
GATTGTAGCGGACAGCGAGCAATTGCAAAATTTTGTGCAAAATACACCGCTTGTTGATGTTATCAATAGCTATCCGGCAG
ATCTATCGGCAGATCAGTTGCTTGCCTTACTGCGCCCACTAACCCCTCGTTTATATTCGATTGCCTCCGCACAGTCGGAA
GTCGGTGAAGAAGTACATCTCAGTGTCGGCGTTGTCCGTTACGACTATAACGGCAAAGCACGTGCCGGCGCTGCATCCAG
CTATTTAGCCGATCGTGTTGAAGAAGACGGGCAAGTACGGATCTTTGTTGAACACAATGACAACTTCAAATTACCGCAAG
ATTCCTCTAAACCGATCATTATGATTGGCTCCGGTACCGGCATTGCACCGTTCAGATCTTTTGTACAACAACGGGCTACT
GATGAAGCGGAAGGTAAAAACTGGCTGATTTTCGGTAATCAACATTTCGCTTCCGATTTCCTATACCAAACCGAATGGCA
ACAATTTGCTAAAGACGGCTTCCTACATAAATACAGCTTTGCATGGTCACGAGATCAGGAGCAAAAAATTTATGTACAAG
ACAAAATCCGTGAAGAAGCCGAGACATTATGGCAATGGTTACAACAAGGGGCTTATCTCTATGTGTGCGGCGATGCTAGC
CGTATGGCGAAAGATGTTAATCAAGCCTTATTAGATGTAATTGCACAGCAAGGCAGTCTGAATGCGGATGAAGCGGAAGA
ATACTTAGATAACTTACGTGAAGAAAAACGTTATCAACGTGATGTTTATTAA

Upstream 100 bases:

>100_bases
TCCATTCTGCTCTAAACTATGCTCTGTTAGTATTTTTAAGAATAACAAAATCGCTCTATAAATACGCTATTGAAGTATTT
TTAAATGTGGGGGATTTTAT

Downstream 100 bases:

>100_bases
AAATTAGTGGAATTTAGGAGTGAGAAAATGAGTGATAAAAAAATCAAAGGCTTAGAGTGGCAAGAAAAACCGTTATCTGA
CAACGAGCGTTTAAAAACTG

Product: sulfite reductase [NADPH] flavoprotein alpha-component (SIR-FP)

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MSLKTTGELPLSAETVQVLANLDHLQLAWLSGYAWAKAQSTNSIGENFAKDSSNLTALVASEPLKVTVLSASQTGNAKSV
ADKLAERLTAEGVNVVRTSLKDYKAKNIADEQLVLLVTSTQGEGEAPEEGVVLLKLLNGKKAPKLDRLQFAVLGLGDSSY
PNFCQAGKDFDQRFYDLGVTRLFERVDADLDFQATADQWINEIVEIIKAKNNEGVATNLTPNASQTPVVTNQSKYNKANP
FPATLITNQRITAKDAEKDVRHLEFDLSGSGLSYQSGDVLGVYFENDPALANEILTALGLSAEEQVTLQDQTLPLSTALQ
TQFELTQNTAAFVKNYAALANHAELNAIVADSEQLQNFVQNTPLVDVINSYPADLSADQLLALLRPLTPRLYSIASAQSE
VGEEVHLSVGVVRYDYNGKARAGAASSYLADRVEEDGQVRIFVEHNDNFKLPQDSSKPIIMIGSGTGIAPFRSFVQQRAT
DEAEGKNWLIFGNQHFASDFLYQTEWQQFAKDGFLHKYSFAWSRDQEQKIYVQDKIREEAETLWQWLQQGAYLYVCGDAS
RMAKDVNQALLDVIAQQGSLNADEAEEYLDNLREEKRYQRDVY

Sequences:

>Translated_603_residues
MSLKTTGELPLSAETVQVLANLDHLQLAWLSGYAWAKAQSTNSIGENFAKDSSNLTALVASEPLKVTVLSASQTGNAKSV
ADKLAERLTAEGVNVVRTSLKDYKAKNIADEQLVLLVTSTQGEGEAPEEGVVLLKLLNGKKAPKLDRLQFAVLGLGDSSY
PNFCQAGKDFDQRFYDLGVTRLFERVDADLDFQATADQWINEIVEIIKAKNNEGVATNLTPNASQTPVVTNQSKYNKANP
FPATLITNQRITAKDAEKDVRHLEFDLSGSGLSYQSGDVLGVYFENDPALANEILTALGLSAEEQVTLQDQTLPLSTALQ
TQFELTQNTAAFVKNYAALANHAELNAIVADSEQLQNFVQNTPLVDVINSYPADLSADQLLALLRPLTPRLYSIASAQSE
VGEEVHLSVGVVRYDYNGKARAGAASSYLADRVEEDGQVRIFVEHNDNFKLPQDSSKPIIMIGSGTGIAPFRSFVQQRAT
DEAEGKNWLIFGNQHFASDFLYQTEWQQFAKDGFLHKYSFAWSRDQEQKIYVQDKIREEAETLWQWLQQGAYLYVCGDAS
RMAKDVNQALLDVIAQQGSLNADEAEEYLDNLREEKRYQRDVY
>Mature_602_residues
SLKTTGELPLSAETVQVLANLDHLQLAWLSGYAWAKAQSTNSIGENFAKDSSNLTALVASEPLKVTVLSASQTGNAKSVA
DKLAERLTAEGVNVVRTSLKDYKAKNIADEQLVLLVTSTQGEGEAPEEGVVLLKLLNGKKAPKLDRLQFAVLGLGDSSYP
NFCQAGKDFDQRFYDLGVTRLFERVDADLDFQATADQWINEIVEIIKAKNNEGVATNLTPNASQTPVVTNQSKYNKANPF
PATLITNQRITAKDAEKDVRHLEFDLSGSGLSYQSGDVLGVYFENDPALANEILTALGLSAEEQVTLQDQTLPLSTALQT
QFELTQNTAAFVKNYAALANHAELNAIVADSEQLQNFVQNTPLVDVINSYPADLSADQLLALLRPLTPRLYSIASAQSEV
GEEVHLSVGVVRYDYNGKARAGAASSYLADRVEEDGQVRIFVEHNDNFKLPQDSSKPIIMIGSGTGIAPFRSFVQQRATD
EAEGKNWLIFGNQHFASDFLYQTEWQQFAKDGFLHKYSFAWSRDQEQKIYVQDKIREEAETLWQWLQQGAYLYVCGDASR
MAKDVNQALLDVIAQQGSLNADEAEEYLDNLREEKRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI24041029, Length=602, Percent_Identity=31.3953488372093, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI7657393, Length=596, Percent_Identity=29.5302013422819, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI221316709, Length=596, Percent_Identity=29.5302013422819, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI221316705, Length=605, Percent_Identity=29.0909090909091, Blast_Score=234, Evalue=2e-61,
Organism=Homo sapiens, GI127139033, Length=606, Percent_Identity=29.3729372937294, Blast_Score=229, Evalue=7e-60,
Organism=Homo sapiens, GI10835173, Length=664, Percent_Identity=29.066265060241, Blast_Score=210, Evalue=3e-54,
Organism=Homo sapiens, GI221316707, Length=528, Percent_Identity=29.5454545454545, Blast_Score=201, Evalue=2e-51,
Organism=Homo sapiens, GI40254422, Length=665, Percent_Identity=27.5187969924812, Blast_Score=182, Evalue=9e-46,
Organism=Homo sapiens, GI169790956, Length=436, Percent_Identity=29.1284403669725, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI169790958, Length=436, Percent_Identity=29.1284403669725, Blast_Score=149, Evalue=7e-36,
Organism=Escherichia coli, GI1789123, Length=602, Percent_Identity=53.3222591362126, Blast_Score=644, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554134, Length=613, Percent_Identity=27.5693311582382, Blast_Score=232, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI17566446, Length=591, Percent_Identity=25.5499153976311, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17531441, Length=267, Percent_Identity=26.5917602996255, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32563863, Length=122, Percent_Identity=31.9672131147541, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321832, Length=675, Percent_Identity=27.1111111111111, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321143, Length=388, Percent_Identity=31.1855670103093, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6325305, Length=633, Percent_Identity=23.5387045813586, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24583543, Length=677, Percent_Identity=28.9512555391433, Blast_Score=206, Evalue=5e-53,
Organism=Drosophila melanogaster, GI78706876, Length=677, Percent_Identity=28.9512555391433, Blast_Score=206, Evalue=5e-53,
Organism=Drosophila melanogaster, GI78706872, Length=677, Percent_Identity=28.9512555391433, Blast_Score=206, Evalue=5e-53,
Organism=Drosophila melanogaster, GI17137192, Length=619, Percent_Identity=26.0096930533118, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24582192, Length=516, Percent_Identity=26.1627906976744, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24660907, Length=595, Percent_Identity=27.563025210084, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24660903, Length=595, Percent_Identity=27.563025210084, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24647438, Length=233, Percent_Identity=24.8927038626609, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 66694; Mature: 66563

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS50902 FLAVODOXIN_LIKE ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKTTGELPLSAETVQVLANLDHLQLAWLSGYAWAKAQSTNSIGENFAKDSSNLTALVA
CCCCCCCCCCCCHHHHHHHHCCCHHEEEECCCCEEECCCCCHHHHHHHHCCCCCEEEEEE
SEPLKVTVLSASQTGNAKSVADKLAERLTAEGVNVVRTSLKDYKAKNIADEQLVLLVTST
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEEC
QGEGEAPEEGVVLLKLLNGKKAPKLDRLQFAVLGLGDSSYPNFCQAGKDFDQRFYDLGVT
CCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHH
RLFERVDADLDFQATADQWINEIVEIIKAKNNEGVATNLTPNASQTPVVTNQSKYNKANP
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEECCCCCCCCCC
FPATLITNQRITAKDAEKDVRHLEFDLSGSGLSYQSGDVLGVYFENDPALANEILTALGL
CCEEEEECCCCCCCHHHHHHHEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCC
SAEEQVTLQDQTLPLSTALQTQFELTQNTAAFVKNYAALANHAELNAIVADSEQLQNFVQ
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCHHHHHHHHH
NTPLVDVINSYPADLSADQLLALLRPLTPRLYSIASAQSEVGEEVHLSVGVVRYDYNGKA
CCCHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCC
RAGAASSYLADRVEEDGQVRIFVEHNDNFKLPQDSSKPIIMIGSGTGIAPFRSFVQQRAT
CCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCC
DEAEGKNWLIFGNQHFASDFLYQTEWQQFAKDGFLHKYSFAWSRDQEQKIYVQDKIREEA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEHHHHHHHHHH
ETLWQWLQQGAYLYVCGDASRMAKDVNQALLDVIAQQGSLNADEAEEYLDNLREEKRYQR
HHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
DVY
CCC
>Mature Secondary Structure 
SLKTTGELPLSAETVQVLANLDHLQLAWLSGYAWAKAQSTNSIGENFAKDSSNLTALVA
CCCCCCCCCCCHHHHHHHHCCCHHEEEECCCCEEECCCCCHHHHHHHHCCCCCEEEEEE
SEPLKVTVLSASQTGNAKSVADKLAERLTAEGVNVVRTSLKDYKAKNIADEQLVLLVTST
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEEC
QGEGEAPEEGVVLLKLLNGKKAPKLDRLQFAVLGLGDSSYPNFCQAGKDFDQRFYDLGVT
CCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHH
RLFERVDADLDFQATADQWINEIVEIIKAKNNEGVATNLTPNASQTPVVTNQSKYNKANP
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEECCCCCCCCCC
FPATLITNQRITAKDAEKDVRHLEFDLSGSGLSYQSGDVLGVYFENDPALANEILTALGL
CCEEEEECCCCCCCHHHHHHHEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCC
SAEEQVTLQDQTLPLSTALQTQFELTQNTAAFVKNYAALANHAELNAIVADSEQLQNFVQ
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCHHHHHHHHH
NTPLVDVINSYPADLSADQLLALLRPLTPRLYSIASAQSEVGEEVHLSVGVVRYDYNGKA
CCCHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCC
RAGAASSYLADRVEEDGQVRIFVEHNDNFKLPQDSSKPIIMIGSGTGIAPFRSFVQQRAT
CCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCC
DEAEGKNWLIFGNQHFASDFLYQTEWQQFAKDGFLHKYSFAWSRDQEQKIYVQDKIREEA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEHHHHHHHHHH
ETLWQWLQQGAYLYVCGDASRMAKDVNQALLDVIAQQGSLNADEAEEYLDNLREEKRYQR
HHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
DVY
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA