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The map label for this gene is asnA

Identifier: 126209299

GI number: 126209299

Start: 2061014

End: 2062006

Strand: Reverse

Name: asnA

Synonym: APL_1837

Alternate gene names: 126209299

Gene position: 2062006-2061014 (Counterclockwise)

Preceding gene: 126209304

Following gene: 126209297

Centisome position: 90.66

GC content: 41.79

Gene sequence:

>993_bases
ATGAAAAAGACATTCATTTTACAGCAACAAGAAATCAGCTTTGTGAAAAACACCTTTACCCAATATTTAATTGATAAATT
AGGCATTATTGAGGTGCAAGGTCCTATCCTCAGCCAAGTGGGTAACGGTATGCAAGATAATCTTTCCGGTATCGAAAAAG
CGGTTCAAGTCAATGTAAAATGTATTCCGGGTGCAACATTTGAAGTGGTGCACTCACTCGCAAAATGGAAACGTCACACT
TTAGCTCGTTTCGGTTTTAAAGAAGGCGAAGGCTTATTCGTACATATGAAAGCATTACGTCCGGACGAAGATTCTCTTGA
CCCGACTCACTCTGTGTATGTGGACCAATGGGACTGGGAAAAAGTGATTCCTGAAGGCCGTCGTAACTTTGATTTCTTAA
AAGAAACCGTAAATGAAATTTATAAAGCGATTCGTTTAACCGAATTAGCCGTGGAAGCACGTTTTGATATTCCGTCAATC
CTACCGAAACAAATTACCTTCGTACACAGTGAAGAATTAGTACAACGTTATCCGAATATGACCGGCAAAGAACGTGAAAA
CGCGATCTGTAAAGAACACGGTGCGGTATTCTTAATCGGTATCGGCGGTAAATTGTCCGACGGTAAACCGCACGACGGAC
GTGCGCCGGACTATGATGACTGGACAACCGAATCTGAAAACGGTTACAAAGGCTTAAACGGCGATATTTTAGTATGGAAC
GAACAACTCGGTACGGCATTTGAACTTTCGTCAATGGGTATCCGTGTTGATGAAAAAGCACTGCGTTTACAAGTCGAATT
AACCGGTGACCAAGATCGTTTAGAAATGGATTGGCATAAAGATTTATTAGCGGGTCGCTTACCGCTATCAATCGGCGGCG
GTATCGGTCAATCCCGTTTAGTGATGTTCTTACTTCGTAAGGCACATATCGGTGAAGTTCAATCGAGCGTATGGCCGAAA
GCAATGTTAGAAAAATATAAAAACATTCTGTAA

Upstream 100 bases:

>100_bases
TTTTATTTGATGAATATTGCATTTTTTACGAATCATATTAAATTACATTCATCTCAACGAATCATGAGAACAAATTACTC
TATTTATAAGGAATAAGATA

Downstream 100 bases:

>100_bases
CTAAAAATTAAGAGCCGATTGCCAGTGTATTCGGCTCTTTTCTTATGGAGGGGTTAATTTGATAGTTTTAGTAATTCCAA
TGTTATTTGCATTATAAGCA

Product: asparagine synthetase AsnA

Products: NA

Alternate protein names: Asparagine synthetase A

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKKTFILQQQEISFVKNTFTQYLIDKLGIIEVQGPILSQVGNGMQDNLSGIEKAVQVNVKCIPGATFEVVHSLAKWKRHT
LARFGFKEGEGLFVHMKALRPDEDSLDPTHSVYVDQWDWEKVIPEGRRNFDFLKETVNEIYKAIRLTELAVEARFDIPSI
LPKQITFVHSEELVQRYPNMTGKERENAICKEHGAVFLIGIGGKLSDGKPHDGRAPDYDDWTTESENGYKGLNGDILVWN
EQLGTAFELSSMGIRVDEKALRLQVELTGDQDRLEMDWHKDLLAGRLPLSIGGGIGQSRLVMFLLRKAHIGEVQSSVWPK
AMLEKYKNIL

Sequences:

>Translated_330_residues
MKKTFILQQQEISFVKNTFTQYLIDKLGIIEVQGPILSQVGNGMQDNLSGIEKAVQVNVKCIPGATFEVVHSLAKWKRHT
LARFGFKEGEGLFVHMKALRPDEDSLDPTHSVYVDQWDWEKVIPEGRRNFDFLKETVNEIYKAIRLTELAVEARFDIPSI
LPKQITFVHSEELVQRYPNMTGKERENAICKEHGAVFLIGIGGKLSDGKPHDGRAPDYDDWTTESENGYKGLNGDILVWN
EQLGTAFELSSMGIRVDEKALRLQVELTGDQDRLEMDWHKDLLAGRLPLSIGGGIGQSRLVMFLLRKAHIGEVQSSVWPK
AMLEKYKNIL
>Mature_330_residues
MKKTFILQQQEISFVKNTFTQYLIDKLGIIEVQGPILSQVGNGMQDNLSGIEKAVQVNVKCIPGATFEVVHSLAKWKRHT
LARFGFKEGEGLFVHMKALRPDEDSLDPTHSVYVDQWDWEKVIPEGRRNFDFLKETVNEIYKAIRLTELAVEARFDIPSI
LPKQITFVHSEELVQRYPNMTGKERENAICKEHGAVFLIGIGGKLSDGKPHDGRAPDYDDWTTESENGYKGLNGDILVWN
EQLGTAFELSSMGIRVDEKALRLQVELTGDQDRLEMDWHKDLLAGRLPLSIGGGIGQSRLVMFLLRKAHIGEVQSSVWPK
AMLEKYKNIL

Specific function: Asparagine biosynthesis. [C]

COG id: COG2502

COG function: function code E; Asparagine synthetase A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily

Homologues:

Organism=Escherichia coli, GI1790183, Length=330, Percent_Identity=62.7272727272727, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASNA_ACTP2 (A3N3D3)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054524.1
- ProteinModelPortal:   A3N3D3
- SMR:   A3N3D3
- STRING:   A3N3D3
- GeneID:   4849781
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1837
- eggNOG:   COG2502
- HOGENOM:   HBG288146
- OMA:   LNDNLNG
- PhylomeDB:   A3N3D3
- ProtClustDB:   PRK05425
- BioCyc:   APLE416269:APL_1837-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00555
- InterPro:   IPR006195
- InterPro:   IPR004618
- PIRSF:   PIRSF001555
- TIGRFAMs:   TIGR00669

Pfam domain/function: PF03590 AsnA

EC number: =6.3.1.1

Molecular weight: Translated: 37421; Mature: 37421

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTFILQQQEISFVKNTFTQYLIDKLGIIEVQGPILSQVGNGMQDNLSGIEKAVQVNVK
CCCCEEECHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHCCCHHHHHHHHHHHHEEEEE
CIPGATFEVVHSLAKWKRHTLARFGFKEGEGLFVHMKALRPDEDSLDPTHSVYVDQWDWE
EECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCEEEECCCCHH
KVIPEGRRNFDFLKETVNEIYKAIRLTELAVEARFDIPSILPKQITFVHSEELVQRYPNM
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCHHHHHHHHHHHHHHHCCCC
TGKERENAICKEHGAVFLIGIGGKLSDGKPHDGRAPDYDDWTTESENGYKGLNGDILVWN
CCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
EQLGTAFELSSMGIRVDEKALRLQVELTGDQDRLEMDWHKDLLAGRLPLSIGGGIGQSRL
CCCCCEEEHHHCCCEEECCEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEECCCCCHHHH
VMFLLRKAHIGEVQSSVWPKAMLEKYKNIL
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MKKTFILQQQEISFVKNTFTQYLIDKLGIIEVQGPILSQVGNGMQDNLSGIEKAVQVNVK
CCCCEEECHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHCCCHHHHHHHHHHHHEEEEE
CIPGATFEVVHSLAKWKRHTLARFGFKEGEGLFVHMKALRPDEDSLDPTHSVYVDQWDWE
EECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCEEEECCCCHH
KVIPEGRRNFDFLKETVNEIYKAIRLTELAVEARFDIPSILPKQITFVHSEELVQRYPNM
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCHHHHHHHHHHHHHHHCCCC
TGKERENAICKEHGAVFLIGIGGKLSDGKPHDGRAPDYDDWTTESENGYKGLNGDILVWN
CCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
EQLGTAFELSSMGIRVDEKALRLQVELTGDQDRLEMDWHKDLLAGRLPLSIGGGIGQSRL
CCCCCEEEHHHCCCEEECCEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEECCCCCHHHH
VMFLLRKAHIGEVQSSVWPKAMLEKYKNIL
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA