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The map label for this gene is syp

Identifier: 126209292

GI number: 126209292

Start: 2052678

End: 2054393

Strand: Direct

Name: syp

Synonym: APL_1830

Alternate gene names: 126209292

Gene position: 2052678-2054393 (Clockwise)

Preceding gene: 126209284

Following gene: 126209294

Centisome position: 90.25

GC content: 44.76

Gene sequence:

>1716_bases
ATGCGTACAAGTCAGTATTTATTTTCGACCCTTAAAGAAACGCCGAATGATGCACAGGTGGTGAGCCATCAATTAATGTT
GCGTGCCGGTATGATTCGTCCGATGGCTTCAGGTTTATATAACTGGTTGCCAACCGGGATCAAAGTGTTGAAAAAAGTAG
AAAATATCATTCGTGAAGAGATGAACAAAGGTGGTGCTATCGAGGTGTTAATGCCTGTGGTGCAACCGGCGGAATTATGG
CAAGAATCAGGTCGTTGGAACGATTACGGTGCGGAATTATTGCGTTTTGTTGATCGTGGCAGCCGTGATTTCGTGTTAGG
CCCGACTCATGAAGAAGTGATTACCGACTTAGTTCGCCGTGAAGTGTCGTCTTACAAACAACTTCCGTTAAATTTGTACC
AAATCCAAACTAAATTCCGTGATGAAGTGCGTCCACGTTTCGGTGTGATGCGTTCACGTGAGTTTGTGATGAAAGATGCG
TATTCTTTCCATGTGGACAAAGCGTCATTACAAGAGACTTATGATGTAATGTACCAAGTGTATAGCAATATTTTCACCCG
TCTTGGTTTAGATTTCCGTGCGGTGCAAGCGGATACCGGTTCAATCGGTGGTTCGGCTTCACACGAATTCCAAGTGTTAG
CGTCAAGCGGTGAAGACGATGTGGTGTTTTCAACCGAATCGGATTTCGCAGCGAATATCGAATTAGCGGAAGCGGTAGCA
GTAGGTGAACGCCAAGCACCAACGGCAGAAATGCAATTAGTTGATACGCCAAATGCGAAAACAATTAACGAATTGGTTGA
GCAATTCAATTTACCGATTGGGAAAACGGTAAAAACTTTAATCGTAAAAGGGGCAACCGAAGAACAGCCGCTTGTGGCGT
TAGTGTTACGTGGCGATCACGAATTAAATGAAATCAAAGCGCAAAAACATCCGTTAGTGGCTGATCCGCTTGAATTTGCA
GATGAAGCGGAAATTAAAGCGAAAATCGGCGCAGGCGTGGGTTCACTTGGCGTAATTAATCTGAATGTTCCGGCGATTAT
CGATCGTTCGGTGGCGGTGATGTCAGATTTCGGCTGTGGTGCAAATATTGACGGTAAACATTATTTCAATGTGAATTGGG
AACGTGATGCAGCAATGCCGGAAGTGGCGGACTTACGTAACGTGGTGGAAGGCGATCCAAGCCCGGACGGCAAAGGCGTG
TTACAAATTAAACGCGGTATTGAAGTCGGTCATATTTTCCAACTTGGTACCAAATATTCGGAAGCGATGAAAGCGACCGT
GCAAGGCGAAGACGGTAAACCGCTCGTGATGACAATGGGTTGCTATGGTATCGGTGTAACACGTGTGGTTGCCGCAGCAA
TCGAACAGCATCACGATGAGCGTGGTATTATTTGGCCATCAGATGAAATCGCCCCATTCACCGTTGCAATCGTGCCGATG
AATATGCACAAATCGGAAAGCGTACAACAATTCTCGGAAGAATTGTATCGCACCTTAAAAGCACAAGGCGTTGATGTAAT
TTTCGATGATCGTAAAGAACGTCCGGGTGTGATGTTTGCCGATATGGAATTAATCGGTGTACCGCATATGGTGGTTATCG
GTGAGAAAAATCTCGCAAACGGTGAAATTGAATACAAAAACCGTCGTACCGGTGAGAAACAAATGATTGCTAAAGATCAG
CTTTTAGCTTTCTTAAAAGAAAATGTGAAAGCATAA

Upstream 100 bases:

>100_bases
AGAAATTTTTTGCAAATTGCAAAATTCTGTGAAAATTCGACCGCTTTTGTTTTAAAATAGATCCCATTTTTCGATATTTA
AAATAAAAATAGGAATAACA

Downstream 100 bases:

>100_bases
TTCGCAAAAAATAGATGAAAAAAGACCGCTTGCCTTAAAACAAGCGGTCGTTTTTCATAGGAATGTTACTTCAAATTAGT
TTTATCTTGTTGGCAGTATT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MRTSQYLFSTLKETPNDAQVVSHQLMLRAGMIRPMASGLYNWLPTGIKVLKKVENIIREEMNKGGAIEVLMPVVQPAELW
QESGRWNDYGAELLRFVDRGSRDFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFVMKDA
YSFHVDKASLQETYDVMYQVYSNIFTRLGLDFRAVQADTGSIGGSASHEFQVLASSGEDDVVFSTESDFAANIELAEAVA
VGERQAPTAEMQLVDTPNAKTINELVEQFNLPIGKTVKTLIVKGATEEQPLVALVLRGDHELNEIKAQKHPLVADPLEFA
DEAEIKAKIGAGVGSLGVINLNVPAIIDRSVAVMSDFGCGANIDGKHYFNVNWERDAAMPEVADLRNVVEGDPSPDGKGV
LQIKRGIEVGHIFQLGTKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVVAAAIEQHHDERGIIWPSDEIAPFTVAIVPM
NMHKSESVQQFSEELYRTLKAQGVDVIFDDRKERPGVMFADMELIGVPHMVVIGEKNLANGEIEYKNRRTGEKQMIAKDQ
LLAFLKENVKA

Sequences:

>Translated_571_residues
MRTSQYLFSTLKETPNDAQVVSHQLMLRAGMIRPMASGLYNWLPTGIKVLKKVENIIREEMNKGGAIEVLMPVVQPAELW
QESGRWNDYGAELLRFVDRGSRDFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFVMKDA
YSFHVDKASLQETYDVMYQVYSNIFTRLGLDFRAVQADTGSIGGSASHEFQVLASSGEDDVVFSTESDFAANIELAEAVA
VGERQAPTAEMQLVDTPNAKTINELVEQFNLPIGKTVKTLIVKGATEEQPLVALVLRGDHELNEIKAQKHPLVADPLEFA
DEAEIKAKIGAGVGSLGVINLNVPAIIDRSVAVMSDFGCGANIDGKHYFNVNWERDAAMPEVADLRNVVEGDPSPDGKGV
LQIKRGIEVGHIFQLGTKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVVAAAIEQHHDERGIIWPSDEIAPFTVAIVPM
NMHKSESVQQFSEELYRTLKAQGVDVIFDDRKERPGVMFADMELIGVPHMVVIGEKNLANGEIEYKNRRTGEKQMIAKDQ
LLAFLKENVKA
>Mature_571_residues
MRTSQYLFSTLKETPNDAQVVSHQLMLRAGMIRPMASGLYNWLPTGIKVLKKVENIIREEMNKGGAIEVLMPVVQPAELW
QESGRWNDYGAELLRFVDRGSRDFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFVMKDA
YSFHVDKASLQETYDVMYQVYSNIFTRLGLDFRAVQADTGSIGGSASHEFQVLASSGEDDVVFSTESDFAANIELAEAVA
VGERQAPTAEMQLVDTPNAKTINELVEQFNLPIGKTVKTLIVKGATEEQPLVALVLRGDHELNEIKAQKHPLVADPLEFA
DEAEIKAKIGAGVGSLGVINLNVPAIIDRSVAVMSDFGCGANIDGKHYFNVNWERDAAMPEVADLRNVVEGDPSPDGKGV
LQIKRGIEVGHIFQLGTKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVVAAAIEQHHDERGIIWPSDEIAPFTVAIVPM
NMHKSESVQQFSEELYRTLKAQGVDVIFDDRKERPGVMFADMELIGVPHMVVIGEKNLANGEIEYKNRRTGEKQMIAKDQ
LLAFLKENVKA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=248, Percent_Identity=42.741935483871, Blast_Score=223, Evalue=5e-58,
Organism=Escherichia coli, GI1786392, Length=572, Percent_Identity=74.8251748251748, Blast_Score=895, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=216, Percent_Identity=40.7407407407407, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=93, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320931, Length=547, Percent_Identity=31.4442413162706, Blast_Score=240, Evalue=6e-64,
Organism=Drosophila melanogaster, GI24656200, Length=248, Percent_Identity=34.2741935483871, Blast_Score=173, Evalue=3e-43,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_ACTP2 (A3N3C6)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054517.1
- ProteinModelPortal:   A3N3C6
- SMR:   A3N3C6
- STRING:   A3N3C6
- GeneID:   4849609
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1830
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   A3N3C6
- ProtClustDB:   PRK09194
- BioCyc:   APLE416269:APL_1830-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63470; Mature: 63470

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQYLFSTLKETPNDAQVVSHQLMLRAGMIRPMASGLYNWLPTGIKVLKKVENIIREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNKGGAIEVLMPVVQPAELWQESGRWNDYGAELLRFVDRGSRDFVLGPTHEEVITDLVRR
HCCCCCEEEHHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFVMKDAYSFHVDKASLQETYDVMYQV
HHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YSNIFTRLGLDFRAVQADTGSIGGSASHEFQVLASSGEDDVVFSTESDFAANIELAEAVA
HHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCHHHHHHHH
VGERQAPTAEMQLVDTPNAKTINELVEQFNLPIGKTVKTLIVKGATEEQPLVALVLRGDH
HCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHEECCCCCCCEEEEEEECCC
ELNEIKAQKHPLVADPLEFADEAEIKAKIGAGVGSLGVINLNVPAIIDRSVAVMSDFGCG
HHHHHHHHCCCCCCCCHHHCCCCHHHHHHCCCCCCEEEEEECCCHHHCCCHHHHHCCCCC
ANIDGKHYFNVNWERDAAMPEVADLRNVVEGDPSPDGKGVLQIKRGIEVGHIFQLGTKYS
CCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
EAMKATVQGEDGKPLVMTMGCYGIGVTRVVAAAIEQHHDERGIIWPSDEIAPFTVAIVPM
HHHHHEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEE
NMHKSESVQQFSEELYRTLKAQGVDVIFDDRKERPGVMFADMELIGVPHMVVIGEKNLAN
CCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCHHHCCCEEEEECCCCCCC
GEIEYKNRRTGEKQMIAKDQLLAFLKENVKA
CEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTSQYLFSTLKETPNDAQVVSHQLMLRAGMIRPMASGLYNWLPTGIKVLKKVENIIREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNKGGAIEVLMPVVQPAELWQESGRWNDYGAELLRFVDRGSRDFVLGPTHEEVITDLVRR
HCCCCCEEEHHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFVMKDAYSFHVDKASLQETYDVMYQV
HHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YSNIFTRLGLDFRAVQADTGSIGGSASHEFQVLASSGEDDVVFSTESDFAANIELAEAVA
HHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCHHHHHHHH
VGERQAPTAEMQLVDTPNAKTINELVEQFNLPIGKTVKTLIVKGATEEQPLVALVLRGDH
HCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHEECCCCCCCEEEEEEECCC
ELNEIKAQKHPLVADPLEFADEAEIKAKIGAGVGSLGVINLNVPAIIDRSVAVMSDFGCG
HHHHHHHHCCCCCCCCHHHCCCCHHHHHHCCCCCCEEEEEECCCHHHCCCHHHHHCCCCC
ANIDGKHYFNVNWERDAAMPEVADLRNVVEGDPSPDGKGVLQIKRGIEVGHIFQLGTKYS
CCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
EAMKATVQGEDGKPLVMTMGCYGIGVTRVVAAAIEQHHDERGIIWPSDEIAPFTVAIVPM
HHHHHEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEE
NMHKSESVQQFSEELYRTLKAQGVDVIFDDRKERPGVMFADMELIGVPHMVVIGEKNLAN
CCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCHHHCCCEEEEECCCCCCC
GEIEYKNRRTGEKQMIAKDQLLAFLKENVKA
CEEEECCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA