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The map label for this gene is araD [H]

Identifier: 126209168

GI number: 126209168

Start: 1936300

End: 1936980

Strand: Direct

Name: araD [H]

Synonym: APL_1704

Alternate gene names: 126209168

Gene position: 1936300-1936980 (Clockwise)

Preceding gene: 126209167

Following gene: 126209170

Centisome position: 85.13

GC content: 46.26

Gene sequence:

>681_bases
ATGCGTGATCGCGTATTTAAAGCGAATCTTGAATTACCAAAATATAAATTAGCCACCTTTACTTGGGGTAACGTGAGTGA
AATCGACCGTGAAACCGGTTATGTAGCGATCAAACCTTCCGGTGTGGAATATGCAACGATGAAGCCGGAAGACATTGTCT
TTGTCGATTTACAAGGTAATCGCATATTCGGCGATAAAAAACCGTCGTCGGATACTCCGACCCACTTAGAATTTTACCGC
CAATTCCCGAATATCGGCGGTGTGGTGCATACTCATTCACGTCACGCGACCGCATGGGCGCAAGCCGGTGAAGATATTCT
TGCTTTAGGTACAACACAAGGCGACTACTTCTACGGTTCGGTACCTTGTACACGCCGAATGACACCGGAAGAAATCGCCG
GCGAATACGAATTAGAAACCGGTAAAGTAGTGGTGGAAACCTTCCGTAAACGCGGTATTGATCCGGATATGGTGCCGGGT
GTATTAGTTCACTCGCACGGGCCGTTCACTTGGGGTAAAGATGCGCATGATGCGGTACATAATTCCGTAGTGCTGGAAGA
AGTAGCGTATATGAACTTCGTTAGCCACCAAATTCGTCCGAATATCGGCTCAATGCAACAAGAATTACTCGATAAACACT
ATTTACGTAAACACGGTGCAAACGCCTATTACGGACAATAA

Upstream 100 bases:

>100_bases
AGCCGATTGCGGAAATTATTAATGCCCGCCGTTGGATTGAACAAAAAATGAAAGAGGGTGGTTTCCAATGCTAACAGAAT
TATCTCCAAAATTACTTGCG

Downstream 100 bases:

>100_bases
ATCTTTTTAATGTTGTGTGTGCAAGCGGTCAAAATTGCAGAATTTTTTGCAAATTCGACCGCTTTTTTATCGAACTTATT
AAGCGGTATATTGTTTCCGG

Product: L-ribulose-5-phosphate 4-epimerase

Products: D-xylulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MRDRVFKANLELPKYKLATFTWGNVSEIDRETGYVAIKPSGVEYATMKPEDIVFVDLQGNRIFGDKKPSSDTPTHLEFYR
QFPNIGGVVHTHSRHATAWAQAGEDILALGTTQGDYFYGSVPCTRRMTPEEIAGEYELETGKVVVETFRKRGIDPDMVPG
VLVHSHGPFTWGKDAHDAVHNSVVLEEVAYMNFVSHQIRPNIGSMQQELLDKHYLRKHGANAYYGQ

Sequences:

>Translated_226_residues
MRDRVFKANLELPKYKLATFTWGNVSEIDRETGYVAIKPSGVEYATMKPEDIVFVDLQGNRIFGDKKPSSDTPTHLEFYR
QFPNIGGVVHTHSRHATAWAQAGEDILALGTTQGDYFYGSVPCTRRMTPEEIAGEYELETGKVVVETFRKRGIDPDMVPG
VLVHSHGPFTWGKDAHDAVHNSVVLEEVAYMNFVSHQIRPNIGSMQQELLDKHYLRKHGANAYYGQ
>Mature_226_residues
MRDRVFKANLELPKYKLATFTWGNVSEIDRETGYVAIKPSGVEYATMKPEDIVFVDLQGNRIFGDKKPSSDTPTHLEFYR
QFPNIGGVVHTHSRHATAWAQAGEDILALGTTQGDYFYGSVPCTRRMTPEEIAGEYELETGKVVVETFRKRGIDPDMVPG
VLVHSHGPFTWGKDAHDAVHNSVVLEEVAYMNFVSHQIRPNIGSMQQELLDKHYLRKHGANAYYGQ

Specific function: Probable pentulose-5-phosphate-4-epimerase [H]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786247, Length=227, Percent_Identity=64.3171806167401, Blast_Score=315, Evalue=1e-87,
Organism=Escherichia coli, GI1790008, Length=227, Percent_Identity=62.1145374449339, Blast_Score=310, Evalue=6e-86,
Organism=Escherichia coli, GI1790642, Length=226, Percent_Identity=59.7345132743363, Blast_Score=297, Evalue=3e-82,

Paralogues:

None

Copy number: 136 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303
- InterPro:   IPR004661 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 5.1.3.4

Molecular weight: Translated: 25500; Mature: 25500

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDRVFKANLELPKYKLATFTWGNVSEIDRETGYVAIKPSGVEYATMKPEDIVFVDLQGN
CCCCEEECCCCCCCEEEEEEECCCHHHHHHCCCEEEECCCCCEEEEECCCCEEEEEECCC
RIFGDKKPSSDTPTHLEFYRQFPNIGGVVHTHSRHATAWAQAGEDILALGTTQGDYFYGS
EEECCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCHHHHHHCCCCEEEEECCCCCEEEEC
VPCTRRMTPEEIAGEYELETGKVVVETFRKRGIDPDMVPGVLVHSHGPFTWGKDAHDAVH
CCCCCCCCHHHHCCCEECCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCHHHHHH
NSVVLEEVAYMNFVSHQIRPNIGSMQQELLDKHYLRKHGANAYYGQ
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRDRVFKANLELPKYKLATFTWGNVSEIDRETGYVAIKPSGVEYATMKPEDIVFVDLQGN
CCCCEEECCCCCCCEEEEEEECCCHHHHHHCCCEEEECCCCCEEEEECCCCEEEEEECCC
RIFGDKKPSSDTPTHLEFYRQFPNIGGVVHTHSRHATAWAQAGEDILALGTTQGDYFYGS
EEECCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCHHHHHHCCCCEEEEECCCCCEEEEC
VPCTRRMTPEEIAGEYELETGKVVVETFRKRGIDPDMVPGVLVHSHGPFTWGKDAHDAVH
CCCCCCCCHHHHCCCEECCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCHHHHHH
NSVVLEEVAYMNFVSHQIRPNIGSMQQELLDKHYLRKHGANAYYGQ
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-ribulose 5-phosphate

Specific reaction: L-ribulose 5-phosphate = D-xylulose 5-phosphate

General reaction: Epimerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]