Click here to switch to the map view.

The map label for this gene is hyaB [H]

Identifier: 126208798

GI number: 126208798

Start: 1531080

End: 1532789

Strand: Direct

Name: hyaB [H]

Synonym: APL_1334

Alternate gene names: 126208798

Gene position: 1531080-1532789 (Clockwise)

Preceding gene: 126208797

Following gene: 126208799

Centisome position: 67.32

GC content: 42.28

Gene sequence:

>1710_bases
ATGACAACTAAACAACGTATTACTATCGACCCTGTTACGCGTATCGAAGGTCATTTACGTATCGATTGTGAAATCGAAGA
CGGAAAAGTCACCAATGCTTGGTCATCAGGTACCATGTGGCGAGGCATGGAAAATATTATTCAGGGCAAAGATCCTCGCG
ATGCTTGGATGATTATGCAACGAATTTGCGGCGTTTGTACGACGGTACACGCTATTGTCAGTGTGAGAGCGGTGGAAGAC
GCAATCGGTGCGAAAGTACCGCTAAATGCGCAATATATTCGCAATATGATTTTAGCCGCACATACGATGCATGACCATAT
CGTACATTTTTATCAACTTTCCGCAATGGACTGGGTGGATATTACTTCTGCGCTCGAAGCCGATCCTGAAAAAGCGGAAG
CGATGTTGAAAGGTGTTTCCACATGGTCGCTGAATAGTGCAAACGAATTCCGTAACGTGCAAAATAAATTAAAAGCGTTG
GTTGCAAGCGGGCAATTAGGTATTTTTGCCAACGGATATTTCGGTCATCCGGCGATGAAGTTACCGCCGGAAGTAAATTT
AATTGCGGTCGCTCACTATTTACAAGCGTTGGAATGTCAGCGTGATGCCAACCGTATCGTGGCGTTATTAGGTAGTAAAA
CCCCTCATATTCAAAACTTAGCGATAGGCGGCGTTGCGAACCCAATTAACTTAGACGCCCAATCCGTACTCAATTTAGAA
CGGCTAATGTTCGTGAAAAGTTGTATAGACAAATTGAACGATTTTGTGAACCAAGTTTATAAAATCGATACCGCCGTTAT
TGCCGCACATTATCCTGAATGGTTACAACTAGGAAAAACATCACGTAACTATTTATCCGTACCGGAATATCCTTTAAATG
AAGATAATACGAAATTCGTACTTTCCGGCGGTTACATTGAAAACGGAGATCTATCTACTTTCCGCAATATTGCCGACTAT
AAAGACGAATTTCTCTTAAAAGGGATTAAAGAAAGCGGCAAACATGCTTGGTATGAAGATGATGAAGCTTTAGAACCGTG
GGCGGGATTAACCCGTCCTAAATATACCGGATGGCAAGAAGACGGAAAATATTCTTGGGTAAAAGCCCCTTCTTTCTATA
ATAAAGTCGTTGAAGTCGGTCCTTTAGCTTATCTTATGTGCGGATTGGCAACCGAAAACAAATTAACCGTAAAACACTTC
AATGAAGTGAAAAACTTATATAAAACTCTCACGCAAAACGAATTATCCGTTGATGATTTGCATTCTACCTTAGGACGAAT
CATCGGACGTACCGTACATTGTTGTACGCTTAACGATTTACTTGTTCAACAATGGCAATTGCTGATAGAGAATATCGGTA
GAGGCGATACCGATGCTTACTTAAAAGCGGAAATTCCGCCGACAGGCGAATTTAAAGGTGTTGGTTTTAGCGAAGTACCG
AGAGGTATGCTATCTCATTGGATTGTAATCAAAGACGGTAAAATCGCGAATTATCAAGCCGTAGTACCGTCCACTTGGAA
CTCCGGTCCGCGCAACCAAAACGATGAAATGGGACCTTATGAGCTATCTTTAATCGGTACGCCTGTTGCTGATCCGAAGA
AACCGTTAGAAATCGTTCGTACGATTCACTCGTTCGACCCTTGTATGTCTTGTGCCGTACATGTCGTTAATACCGAAACG
GACGAAGTAACCGAGGTTAAGGTACTATAA

Upstream 100 bases:

>100_bases
TTGAAATTTGCGCCTATATATTAATTGTTAGATTATTCCCTGTATTACCAGTTATCCAAGAAGCACATCAAGAAAATAAT
ATTCAATCCGGAGTGAGAGC

Downstream 100 bases:

>100_bases
TGAAACCGTTAATCTTAGGAATCGGAAACATTTTATTAAGCGATGAGGGTGTCGGTGTCAGAGCAGTTCAGGAACTGGAA
CAAAAACCGGAACTTACGGC

Product: hydrogenase 2 large subunit

Products: NA

Alternate protein names: HYD2; Membrane-bound hydrogenase 2 large subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MTTKQRITIDPVTRIEGHLRIDCEIEDGKVTNAWSSGTMWRGMENIIQGKDPRDAWMIMQRICGVCTTVHAIVSVRAVED
AIGAKVPLNAQYIRNMILAAHTMHDHIVHFYQLSAMDWVDITSALEADPEKAEAMLKGVSTWSLNSANEFRNVQNKLKAL
VASGQLGIFANGYFGHPAMKLPPEVNLIAVAHYLQALECQRDANRIVALLGSKTPHIQNLAIGGVANPINLDAQSVLNLE
RLMFVKSCIDKLNDFVNQVYKIDTAVIAAHYPEWLQLGKTSRNYLSVPEYPLNEDNTKFVLSGGYIENGDLSTFRNIADY
KDEFLLKGIKESGKHAWYEDDEALEPWAGLTRPKYTGWQEDGKYSWVKAPSFYNKVVEVGPLAYLMCGLATENKLTVKHF
NEVKNLYKTLTQNELSVDDLHSTLGRIIGRTVHCCTLNDLLVQQWQLLIENIGRGDTDAYLKAEIPPTGEFKGVGFSEVP
RGMLSHWIVIKDGKIANYQAVVPSTWNSGPRNQNDEMGPYELSLIGTPVADPKKPLEIVRTIHSFDPCMSCAVHVVNTET
DEVTEVKVL

Sequences:

>Translated_569_residues
MTTKQRITIDPVTRIEGHLRIDCEIEDGKVTNAWSSGTMWRGMENIIQGKDPRDAWMIMQRICGVCTTVHAIVSVRAVED
AIGAKVPLNAQYIRNMILAAHTMHDHIVHFYQLSAMDWVDITSALEADPEKAEAMLKGVSTWSLNSANEFRNVQNKLKAL
VASGQLGIFANGYFGHPAMKLPPEVNLIAVAHYLQALECQRDANRIVALLGSKTPHIQNLAIGGVANPINLDAQSVLNLE
RLMFVKSCIDKLNDFVNQVYKIDTAVIAAHYPEWLQLGKTSRNYLSVPEYPLNEDNTKFVLSGGYIENGDLSTFRNIADY
KDEFLLKGIKESGKHAWYEDDEALEPWAGLTRPKYTGWQEDGKYSWVKAPSFYNKVVEVGPLAYLMCGLATENKLTVKHF
NEVKNLYKTLTQNELSVDDLHSTLGRIIGRTVHCCTLNDLLVQQWQLLIENIGRGDTDAYLKAEIPPTGEFKGVGFSEVP
RGMLSHWIVIKDGKIANYQAVVPSTWNSGPRNQNDEMGPYELSLIGTPVADPKKPLEIVRTIHSFDPCMSCAVHVVNTET
DEVTEVKVL
>Mature_568_residues
TTKQRITIDPVTRIEGHLRIDCEIEDGKVTNAWSSGTMWRGMENIIQGKDPRDAWMIMQRICGVCTTVHAIVSVRAVEDA
IGAKVPLNAQYIRNMILAAHTMHDHIVHFYQLSAMDWVDITSALEADPEKAEAMLKGVSTWSLNSANEFRNVQNKLKALV
ASGQLGIFANGYFGHPAMKLPPEVNLIAVAHYLQALECQRDANRIVALLGSKTPHIQNLAIGGVANPINLDAQSVLNLER
LMFVKSCIDKLNDFVNQVYKIDTAVIAAHYPEWLQLGKTSRNYLSVPEYPLNEDNTKFVLSGGYIENGDLSTFRNIADYK
DEFLLKGIKESGKHAWYEDDEALEPWAGLTRPKYTGWQEDGKYSWVKAPSFYNKVVEVGPLAYLMCGLATENKLTVKHFN
EVKNLYKTLTQNELSVDDLHSTLGRIIGRTVHCCTLNDLLVQQWQLLIENIGRGDTDAYLKAEIPPTGEFKGVGFSEVPR
GMLSHWIVIKDGKIANYQAVVPSTWNSGPRNQNDEMGPYELSLIGTPVADPKKPLEIVRTIHSFDPCMSCAVHVVNTETD
EVTEVKVL

Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=565, Percent_Identity=72.212389380531, Blast_Score=875, Evalue=0.0,
Organism=Escherichia coli, GI1787207, Length=593, Percent_Identity=41.6526138279933, Blast_Score=452, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 63610; Mature: 63479

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKQRITIDPVTRIEGHLRIDCEIEDGKVTNAWSSGTMWRGMENIIQGKDPRDAWMIMQ
CCCCCEEEECCHHHCCCEEEEEEEECCCEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHH
RICGVCTTVHAIVSVRAVEDAIGAKVPLNAQYIRNMILAAHTMHDHIVHFYQLSAMDWVD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ITSALEADPEKAEAMLKGVSTWSLNSANEFRNVQNKLKALVASGQLGIFANGYFGHPAMK
HHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCC
LPPEVNLIAVAHYLQALECQRDANRIVALLGSKTPHIQNLAIGGVANPINLDAQSVLNLE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
RLMFVKSCIDKLNDFVNQVYKIDTAVIAAHYPEWLQLGKTSRNYLSVPEYPLNEDNTKFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEE
LSGGYIENGDLSTFRNIADYKDEFLLKGIKESGKHAWYEDDEALEPWAGLTRPKYTGWQE
EECCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DGKYSWVKAPSFYNKVVEVGPLAYLMCGLATENKLTVKHFNEVKNLYKTLTQNELSVDDL
CCCEEEEECCHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
HSTLGRIIGRTVHCCTLNDLLVQQWQLLIENIGRGDTDAYLKAEIPPTGEFKGVGFSEVP
HHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHH
RGMLSHWIVIKDGKIANYQAVVPSTWNSGPRNQNDEMGPYELSLIGTPVADPKKPLEIVR
HHHHHCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHH
TIHSFDPCMSCAVHVVNTETDEVTEVKVL
HHHHCCHHHHHEEEEEECCCCCCEEEEEC
>Mature Secondary Structure 
TTKQRITIDPVTRIEGHLRIDCEIEDGKVTNAWSSGTMWRGMENIIQGKDPRDAWMIMQ
CCCCEEEECCHHHCCCEEEEEEEECCCEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHH
RICGVCTTVHAIVSVRAVEDAIGAKVPLNAQYIRNMILAAHTMHDHIVHFYQLSAMDWVD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ITSALEADPEKAEAMLKGVSTWSLNSANEFRNVQNKLKALVASGQLGIFANGYFGHPAMK
HHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCC
LPPEVNLIAVAHYLQALECQRDANRIVALLGSKTPHIQNLAIGGVANPINLDAQSVLNLE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
RLMFVKSCIDKLNDFVNQVYKIDTAVIAAHYPEWLQLGKTSRNYLSVPEYPLNEDNTKFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEE
LSGGYIENGDLSTFRNIADYKDEFLLKGIKESGKHAWYEDDEALEPWAGLTRPKYTGWQE
EECCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DGKYSWVKAPSFYNKVVEVGPLAYLMCGLATENKLTVKHFNEVKNLYKTLTQNELSVDDL
CCCEEEEECCHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
HSTLGRIIGRTVHCCTLNDLLVQQWQLLIENIGRGDTDAYLKAEIPPTGEFKGVGFSEVP
HHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHH
RGMLSHWIVIKDGKIANYQAVVPSTWNSGPRNQNDEMGPYELSLIGTPVADPKKPLEIVR
HHHHHCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHH
TIHSFDPCMSCAVHVVNTETDEVTEVKVL
HHHHCCHHHHHEEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]