Click here to switch to the map view.

The map label for this gene is hybD [H]

Identifier: 126208799

GI number: 126208799

Start: 1532789

End: 1533286

Strand: Direct

Name: hybD [H]

Synonym: APL_1335

Alternate gene names: 126208799

Gene position: 1532789-1533286 (Clockwise)

Preceding gene: 126208798

Following gene: 126208800

Centisome position: 67.39

GC content: 40.96

Gene sequence:

>498_bases
ATGAAACCGTTAATCTTAGGAATCGGAAACATTTTATTAAGCGATGAGGGTGTCGGTGTCAGAGCAGTTCAGGAACTGGA
ACAAAAACCGGAACTTACGGCGTTCTTAGATATTGTTGATGGCGGAACATGCGGAATGGAACTCCTCGATTGTATCGCCA
ATCGCAAACATCTGATTATTATAGATGCGGTACTTGCCAACAAAAATGCGGGAGAAATTATCGTATTGCACAATGAAAAA
ATCCCGACATTTTTTTCACGTAAAATATCGCCGCATCAGCTTGGTATTTGCGATGTATTATCGGCATTGACCTTAACGGA
AGAGTTGCCTGAACATCTATGCCTAATCGGCATCCAACCGGAATCGTTGGAATCCGGAATCGGATTAACTAAAACAATAG
AAAATGCGTTTCCGTCTATTTTTCATTGTTTAGCGCAACAACTGGCGGCTTACGGATTTAATGATCCGTTATTACTTCGA
GAGTTGGAAAATGTATAG

Upstream 100 bases:

>100_bases
AAATCGTTCGTACGATTCACTCGTTCGACCCTTGTATGTCTTGTGCCGTACATGTCGTTAATACCGAAACGGACGAAGTA
ACCGAGGTTAAGGTACTATA

Downstream 100 bases:

>100_bases
ACACCAAAATACATCAATCGATGGCCCATTATCCGAAGTAATGGGGTTTAACGACAATCCGGGAGTATTATTTCGAAAGG
AGATGGAAAAAATATCCGCT

Product: hydrogenase 2 maturation endopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MKPLILGIGNILLSDEGVGVRAVQELEQKPELTAFLDIVDGGTCGMELLDCIANRKHLIIIDAVLANKNAGEIIVLHNEK
IPTFFSRKISPHQLGICDVLSALTLTEELPEHLCLIGIQPESLESGIGLTKTIENAFPSIFHCLAQQLAAYGFNDPLLLR
ELENV

Sequences:

>Translated_165_residues
MKPLILGIGNILLSDEGVGVRAVQELEQKPELTAFLDIVDGGTCGMELLDCIANRKHLIIIDAVLANKNAGEIIVLHNEK
IPTFFSRKISPHQLGICDVLSALTLTEELPEHLCLIGIQPESLESGIGLTKTIENAFPSIFHCLAQQLAAYGFNDPLLLR
ELENV
>Mature_165_residues
MKPLILGIGNILLSDEGVGVRAVQELEQKPELTAFLDIVDGGTCGMELLDCIANRKHLIIIDAVLANKNAGEIIVLHNEK
IPTFFSRKISPHQLGICDVLSALTLTEELPEHLCLIGIQPESLESGIGLTKTIENAFPSIFHCLAQQLAAYGFNDPLLLR
ELENV

Specific function: Protease involved in the C-terminal processing of hybC, the large subunit of hydrogenase 2. Specifically cleaves off a 15 amino acid peptide from the C-terminus of the precursor of hybC [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789367, Length=147, Percent_Identity=54.421768707483, Blast_Score=158, Evalue=2e-40,
Organism=Escherichia coli, GI1787209, Length=139, Percent_Identity=33.8129496402878, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 17977; Mature: 17977

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLILGIGNILLSDEGVGVRAVQELEQKPELTAFLDIVDGGTCGMELLDCIANRKHLII
CCCEEEECCCEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEE
IDAVLANKNAGEIIVLHNEKIPTFFSRKISPHQLGICDVLSALTLTEELPEHLCLIGIQP
EEEEECCCCCCEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCH
ESLESGIGLTKTIENAFPSIFHCLAQQLAAYGFNDPLLLRELENV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC
>Mature Secondary Structure
MKPLILGIGNILLSDEGVGVRAVQELEQKPELTAFLDIVDGGTCGMELLDCIANRKHLII
CCCEEEECCCEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEE
IDAVLANKNAGEIIVLHNEKIPTFFSRKISPHQLGICDVLSALTLTEELPEHLCLIGIQP
EEEEECCCCCCEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCH
ESLESGIGLTKTIENAFPSIFHCLAQQLAAYGFNDPLLLRELENV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8021226; 9278503; 10331925 [H]