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The map label for this gene is hypF [H]

Identifier: 126208794

GI number: 126208794

Start: 1525217

End: 1527502

Strand: Reverse

Name: hypF [H]

Synonym: APL_1330

Alternate gene names: 126208794

Gene position: 1527502-1525217 (Counterclockwise)

Preceding gene: 126208802

Following gene: 126208785

Centisome position: 67.16

GC content: 41.95

Gene sequence:

>2286_bases
ATGGGGAATACGGCGTATAACGGTATTGAAATTCGAGTAAAAGGAAAGGTACAAGGTGTCGGTTTTCGTCCGTTCGTTTG
GCAATTGGCAAATGAACACCATTTATACGGCGATGTAAATAATGACGGGCAAGGTGTTTTGATTAGAATATTAACCGAGA
GCGAAACGAAAAAACTCGATGCCTTTTTATATGATCTACAAAATAAACTTCCGCCATTGGCACAGATTACTTCATTACAA
ATTCAACCTACCGAGTGGGATTCGATACTTGATCCGAATGCATTCCTAATTCGTGAAAGCGAAAACAGTCAAATGGATAC
CCAAGTGATTCCGGATGCTGCGACTTGTCCCGCTTGTCTTAATGAGATCTTTGATATAAATAACCGCCGCTTTCATTACC
CGTTTACCAATTGTACTCACTGCGGTCCTCGTTTTACGATTATTCGTTCGATACCTTATGATCGCCATAACACCTCCATG
CGTGATTTTGCTTTTTGCCCTGTCTGTAAGCAGGAATATAAAGATCCGGCGGACAGACGATTTCACGCGCAGCCTAACGC
TTGTCCCGAATGCGGACCGCATATCATTTTCTGCGATAGAACACAGCGGATTACGCAACACTATTCCGCAATTTTAGCCG
CAGCCGATAAATTACGCCAAGGCTACATTGTTGCCGTTAAAGGATTAGGCGGATTTCATTTGGCTTGTGATGCGACCCAA
GCGAAAACGATAGATTTATTACGTCATAGAAAAGTCAGACCTAGCAAACCTTTTGCGGTAATGGTACCTAATCTTGATAG
GCTAACGGAATTAGCCGAAGAAGAACGTTTATTATTGCAAAGTAGTGCGGCACCGATAGTGTTATTAGCGAAGCATAAAG
TGCGCGGAGTACACGATTTAGTCGCTCCAGATTTAGCTGAAATCGGCGTTATGTTACCTAGCAATCCGTTACAGCATTTG
CTTATGCATGAAGTGAATATGCCTCTGATTATGACTAGCGGAAATCGTAGCGGGTTACCTCCCGCGCTGGATAATCGAGA
TGCTCTCGACTCGTTAGCGGATTTAGCGGATTACTGGTTACTGCATAATCGAGATATAGTACAACGAGCCGATGATTCTC
TTGTTAGGGTATCGTTTGACGGCAAAGAAACGTTACGCAGAGCGCGCGGTTATGTGCCTAACGAAACCGCTTTATCTATT
GAGAACGATAAGAATATTTTGGCATTAGGTGCCGATTTAAAAAATACGTTCTGTTTATTACGTAAGAAAAATGCCGTGAT
TACCCAACACTTAGGAGATATGCAAAATGAGCAAATACAACAGCAATTAATTGATAATATCGCATTATTCGAACAAATTT
ATCAATTTACTCCCGATTTGATCGCCGTTGATGCACACCCCGGTTATTTTACGCATCAACTGGGAAAGACTTTATCCGAA
AAATATCATATTCCGTTGATCGAGGTGTTACATCACCACGCGCATATCGTTAGCGTATTGGCGGAACATAATTATTTGCA
AGAACAAGTTATCGGTTTGGCATTAGACGGTATCGGAATGGGAGAGGATTCCCAATTATGGGGCGGGGAATGTTTATTGG
TTTCTCAAAACGGTAGTCAATATTTAGGCGGGTTGCCTCAAGTTGCTTTGATTGGCGGGGATATTGCCGCTAAACAACCT
TGGCGTAGTTTTCTTGCGCATTGTTTAGCTTTTGTCGATAACTGGCAAAATAATGCACAAGTTCGGCAGATTATTAAAGA
TAAAAATTGGCAGCCTTTGGTTAGAGCGGTTGAGGCTAAAATTAATGCGCCGTTGATTTCTTCAGCAGGTCGTTTATTTG
ATGCGGCAGCCTGTGCATTGGGTATTGTCGCTGATCAGATAACTTGGGAAGGAGAGGCGGCTTGTAAACTGGAAAATATA
GCTTGGCAGTATGCTCAAACGGGAGAAACTTTACGTAACGGTATCACAATCTCTTTAAATGAACATAATCAATTAAATTT
AGCGGATTTTTGGTCGGATTGGTTGGCTTTGGAGGCTTCTTCGGCAGAAAAAGCTTGGATTTTTCATTATTCGTTAGCAC
AAGGATTAGCGAACCTAGCACGTTTACAAGCGGATAAATATCATATTAAAACTATCGTATTGTCCGGAGGTGTGTGGCAT
AACCGATTACTGCGTTATTTAATTAAATCAATGTTATCCGATTTTAAGCTATTGACTGGACATGAATTTCCCATGGGAGA
CGGCGGTATAGCTCTCGGTCAAGCGGTGATCGCAAGCTATGATTAA

Upstream 100 bases:

>100_bases
GTATTTATTGATAAGAATCATTGTTTTCTTGGACGGGGAGTATTAATTTACCGTCGGTTAGTTTAACAAAAGTAAAGTTG
TTTGAAGTGGGGAAATCATC

Downstream 100 bases:

>100_bases
TATTAATGTAAGGGCGAATAAATTCGCCCTTATAAATTACAGTGTTATATTTACGGTATAATATTTCGGATGTTTCTGCT
TAGCAAATACGCGGTAGAGG

Product: carbamoyltransferase HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 761; Mature: 760

Protein sequence:

>761_residues
MGNTAYNGIEIRVKGKVQGVGFRPFVWQLANEHHLYGDVNNDGQGVLIRILTESETKKLDAFLYDLQNKLPPLAQITSLQ
IQPTEWDSILDPNAFLIRESENSQMDTQVIPDAATCPACLNEIFDINNRRFHYPFTNCTHCGPRFTIIRSIPYDRHNTSM
RDFAFCPVCKQEYKDPADRRFHAQPNACPECGPHIIFCDRTQRITQHYSAILAAADKLRQGYIVAVKGLGGFHLACDATQ
AKTIDLLRHRKVRPSKPFAVMVPNLDRLTELAEEERLLLQSSAAPIVLLAKHKVRGVHDLVAPDLAEIGVMLPSNPLQHL
LMHEVNMPLIMTSGNRSGLPPALDNRDALDSLADLADYWLLHNRDIVQRADDSLVRVSFDGKETLRRARGYVPNETALSI
ENDKNILALGADLKNTFCLLRKKNAVITQHLGDMQNEQIQQQLIDNIALFEQIYQFTPDLIAVDAHPGYFTHQLGKTLSE
KYHIPLIEVLHHHAHIVSVLAEHNYLQEQVIGLALDGIGMGEDSQLWGGECLLVSQNGSQYLGGLPQVALIGGDIAAKQP
WRSFLAHCLAFVDNWQNNAQVRQIIKDKNWQPLVRAVEAKINAPLISSAGRLFDAAACALGIVADQITWEGEAACKLENI
AWQYAQTGETLRNGITISLNEHNQLNLADFWSDWLALEASSAEKAWIFHYSLAQGLANLARLQADKYHIKTIVLSGGVWH
NRLLRYLIKSMLSDFKLLTGHEFPMGDGGIALGQAVIASYD

Sequences:

>Translated_761_residues
MGNTAYNGIEIRVKGKVQGVGFRPFVWQLANEHHLYGDVNNDGQGVLIRILTESETKKLDAFLYDLQNKLPPLAQITSLQ
IQPTEWDSILDPNAFLIRESENSQMDTQVIPDAATCPACLNEIFDINNRRFHYPFTNCTHCGPRFTIIRSIPYDRHNTSM
RDFAFCPVCKQEYKDPADRRFHAQPNACPECGPHIIFCDRTQRITQHYSAILAAADKLRQGYIVAVKGLGGFHLACDATQ
AKTIDLLRHRKVRPSKPFAVMVPNLDRLTELAEEERLLLQSSAAPIVLLAKHKVRGVHDLVAPDLAEIGVMLPSNPLQHL
LMHEVNMPLIMTSGNRSGLPPALDNRDALDSLADLADYWLLHNRDIVQRADDSLVRVSFDGKETLRRARGYVPNETALSI
ENDKNILALGADLKNTFCLLRKKNAVITQHLGDMQNEQIQQQLIDNIALFEQIYQFTPDLIAVDAHPGYFTHQLGKTLSE
KYHIPLIEVLHHHAHIVSVLAEHNYLQEQVIGLALDGIGMGEDSQLWGGECLLVSQNGSQYLGGLPQVALIGGDIAAKQP
WRSFLAHCLAFVDNWQNNAQVRQIIKDKNWQPLVRAVEAKINAPLISSAGRLFDAAACALGIVADQITWEGEAACKLENI
AWQYAQTGETLRNGITISLNEHNQLNLADFWSDWLALEASSAEKAWIFHYSLAQGLANLARLQADKYHIKTIVLSGGVWH
NRLLRYLIKSMLSDFKLLTGHEFPMGDGGIALGQAVIASYD
>Mature_760_residues
GNTAYNGIEIRVKGKVQGVGFRPFVWQLANEHHLYGDVNNDGQGVLIRILTESETKKLDAFLYDLQNKLPPLAQITSLQI
QPTEWDSILDPNAFLIRESENSQMDTQVIPDAATCPACLNEIFDINNRRFHYPFTNCTHCGPRFTIIRSIPYDRHNTSMR
DFAFCPVCKQEYKDPADRRFHAQPNACPECGPHIIFCDRTQRITQHYSAILAAADKLRQGYIVAVKGLGGFHLACDATQA
KTIDLLRHRKVRPSKPFAVMVPNLDRLTELAEEERLLLQSSAAPIVLLAKHKVRGVHDLVAPDLAEIGVMLPSNPLQHLL
MHEVNMPLIMTSGNRSGLPPALDNRDALDSLADLADYWLLHNRDIVQRADDSLVRVSFDGKETLRRARGYVPNETALSIE
NDKNILALGADLKNTFCLLRKKNAVITQHLGDMQNEQIQQQLIDNIALFEQIYQFTPDLIAVDAHPGYFTHQLGKTLSEK
YHIPLIEVLHHHAHIVSVLAEHNYLQEQVIGLALDGIGMGEDSQLWGGECLLVSQNGSQYLGGLPQVALIGGDIAAKQPW
RSFLAHCLAFVDNWQNNAQVRQIIKDKNWQPLVRAVEAKINAPLISSAGRLFDAAACALGIVADQITWEGEAACKLENIA
WQYAQTGETLRNGITISLNEHNQLNLADFWSDWLALEASSAEKAWIFHYSLAQGLANLARLQADKYHIKTIVLSGGVWHN
RLLRYLIKSMLSDFKLLTGHEFPMGDGGIALGQAVIASYD

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=763, Percent_Identity=51.9003931847969, Blast_Score=708, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 85059; Mature: 84928

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01016 GLYCOPROTEASE ; PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNTAYNGIEIRVKGKVQGVGFRPFVWQLANEHHLYGDVNNDGQGVLIRILTESETKKLD
CCCCCCCCEEEEEEEEEECCCCCHHHHHHCCCCEEEECCCCCCCEEEEEEEECCCHHHHH
AFLYDLQNKLPPLAQITSLQIQPTEWDSILDPNAFLIRESENSQMDTQVIPDAATCPACL
HHHHHHHHCCCCCHHHHEEEECCCCCCCCCCCCEEEEECCCCCCCCCEECCCCCCCHHHH
NEIFDINNRRFHYPFTNCTHCGPRFTIIRSIPYDRHNTSMRDFAFCPVCKQEYKDPADRR
HHHHCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCHHHEECCHHHHHCCCCHHCE
FHAQPNACPECGPHIIFCDRTQRITQHYSAILAAADKLRQGYIVAVKGLGGFHLACDATQ
ECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCH
AKTIDLLRHRKVRPSKPFAVMVPNLDRLTELAEEERLLLQSSAAPIVLLAKHKVRGVHDL
HHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHCCHHHH
VAPDLAEIGVMLPSNPLQHLLMHEVNMPLIMTSGNRSGLPPALDNRDALDSLADLADYWL
HCCCHHHCCEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
LHNRDIVQRADDSLVRVSFDGKETLRRARGYVPNETALSIENDKNILALGADLKNTFCLL
HCCCHHHHHCCCCEEEEEECCHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCCHHEEEE
RKKNAVITQHLGDMQNEQIQQQLIDNIALFEQIYQFTPDLIAVDAHPGYFTHQLGKTLSE
ECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
KYHIPLIEVLHHHAHIVSVLAEHNYLQEQVIGLALDGIGMGEDSQLWGGECLLVSQNGSQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEEECCCCH
YLGGLPQVALIGGDIAAKQPWRSFLAHCLAFVDNWQNNAQVRQIIKDKNWQPLVRAVEAK
HHCCCCEEEEECCCHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHH
INAPLISSAGRLFDAAACALGIVADQITWEGEAACKLENIAWQYAQTGETLRNGITISLN
CCCCHHHHCHHHHHHHHHHHHHHHHHEEECCCCCEEHHHHHHHHHHHHHHHHCCEEEEEC
EHNQLNLADFWSDWLALEASSAEKAWIFHYSLAQGLANLARLQADKYHIKTIVLSGGVWH
CCCCCCHHHHHHHHHEEECCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEEEEEEECCHHH
NRLLRYLIKSMLSDFKLLTGHEFPMGDGGIALGQAVIASYD
HHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
GNTAYNGIEIRVKGKVQGVGFRPFVWQLANEHHLYGDVNNDGQGVLIRILTESETKKLD
CCCCCCCEEEEEEEEEECCCCCHHHHHHCCCCEEEECCCCCCCEEEEEEEECCCHHHHH
AFLYDLQNKLPPLAQITSLQIQPTEWDSILDPNAFLIRESENSQMDTQVIPDAATCPACL
HHHHHHHHCCCCCHHHHEEEECCCCCCCCCCCCEEEEECCCCCCCCCEECCCCCCCHHHH
NEIFDINNRRFHYPFTNCTHCGPRFTIIRSIPYDRHNTSMRDFAFCPVCKQEYKDPADRR
HHHHCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCHHHEECCHHHHHCCCCHHCE
FHAQPNACPECGPHIIFCDRTQRITQHYSAILAAADKLRQGYIVAVKGLGGFHLACDATQ
ECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCH
AKTIDLLRHRKVRPSKPFAVMVPNLDRLTELAEEERLLLQSSAAPIVLLAKHKVRGVHDL
HHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHCCHHHH
VAPDLAEIGVMLPSNPLQHLLMHEVNMPLIMTSGNRSGLPPALDNRDALDSLADLADYWL
HCCCHHHCCEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
LHNRDIVQRADDSLVRVSFDGKETLRRARGYVPNETALSIENDKNILALGADLKNTFCLL
HCCCHHHHHCCCCEEEEEECCHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCCHHEEEE
RKKNAVITQHLGDMQNEQIQQQLIDNIALFEQIYQFTPDLIAVDAHPGYFTHQLGKTLSE
ECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
KYHIPLIEVLHHHAHIVSVLAEHNYLQEQVIGLALDGIGMGEDSQLWGGECLLVSQNGSQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEEECCCCH
YLGGLPQVALIGGDIAAKQPWRSFLAHCLAFVDNWQNNAQVRQIIKDKNWQPLVRAVEAK
HHCCCCEEEEECCCHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHH
INAPLISSAGRLFDAAACALGIVADQITWEGEAACKLENIAWQYAQTGETLRNGITISLN
CCCCHHHHCHHHHHHHHHHHHHHHHHEEECCCCCEEHHHHHHHHHHHHHHHHCCEEEEEC
EHNQLNLADFWSDWLALEASSAEKAWIFHYSLAQGLANLARLQADKYHIKTIVLSGGVWH
CCCCCCHHHHHHHHHEEECCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEEEEEEECCHHH
NRLLRYLIKSMLSDFKLLTGHEFPMGDGGIALGQAVIASYD
HHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8661925; 12206761 [H]