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The map label for this gene is thiP [H]

Identifier: 126208785

GI number: 126208785

Start: 1513393

End: 1515018

Strand: Reverse

Name: thiP [H]

Synonym: APL_1321

Alternate gene names: 126208785

Gene position: 1515018-1513393 (Counterclockwise)

Preceding gene: 126208794

Following gene: 126208784

Centisome position: 66.61

GC content: 39.18

Gene sequence:

>1626_bases
ATGTTAAAATTTATTCATTCTTTTACGATCAAAGAAAATTTCTCTCGTTTCCTCGCAAGATACTCCGCTTGGTCGGTATA
TGGCATTATTGTTGCGATTTATGCCATGAGCCTGTTTGCATTAATGGCACATCGACAAGACGACAGTATGTGGAGTGTTT
CCGATGTATTACCGATTTTAAAATATAGTTTTTTACAGGCCGGATTATCGGCGGTTCTTACCTGTATGTTAGGTGTTTTA
TTAGCCAGAGCGTTTTTTTATTTGGACTTTATCGGAAAATCTTATCTGTATAAAATTCTTTCCTTTGTTTGGGCGTTACC
TTCGCTTGTGGTTATTTTCGCATTAATCGGATTTTGGGGAAATTCGGGATGGATAGCGGCATTTCTACAATGGTTGGGTA
TTGAGTGGCAATTTCAACTATATGGTTTACAAGGCATTTTGCTCGCCCATGTTTTTTTTAATGTGCCGTTAGTGACAAAA
TATTGTGTGGAGAGTTTAACTCTTATTCCAAGCAGCCAACATCGTTTAGCCGCACAAATGAATCTGAAAGGCTGGTCTTA
TTTTAAGATCGTCGAATTACCGATGTTAAGCGGGACTTTACCCTATGCTTTTGCAAACGTCTTTTTAATTTGTTTTACCA
GTTTCCCTATCGTACTAATGTTAGGCGGCAGTCCGAAATACAGCACATTGGAAGTCGCTATCTATCAAGCGGTGACTTTT
GAGTTTGATTTTGCAAAAGCGGTCATATTGATTATGGTGCAATTAGCGGTAGGTATCTTGTTGCAACTGCTAATGGACTT
TACCACTAAGTATGCCTTTAACAACGCCAAACAATATACGCCGGTGGTGGATATGATTTGGAAACCTAACCCGATGGGAT
GGCAAAAGAAAGGGTTGCAAATCGTACTGCTGCTGCAAAGTTTATTGATTATTTTACCGCTTACCAGTGTGGTTTGGGCC
GGTATTTCCGTTTCCAATTTCAGTGAGCGATTGTTAAATTCGGCGTTATGGGAAGCCGCTTGGTTTTCATTCTTACTCAG
TCTGATTGCCTCTTGTTCGGTGTTATTTATTGCCTATTTACTAGCCTTGGAAACCAGACAATTGGCATTTAAACATCAAA
AATGGCAATACGGTATTTTGAACGGTGTTGCGACTTATCCTTTGATTTTACCGGTATTTCTATTAGCGGTCGGTTTATTC
CTATTATTGATGGATGTTGAATTAAGCACATTACATTTATTATGGTTAGTCGGGATTTGTAACGGTTTAACCTTATTACC
CTATATTTATCGTCTTATTTTTGCCGCAATGTGGCATTCACTTACCGCACAAGATAAATTAGCGCGCAGTTTAGGGCTAA
GCGGGTTTCGCCGTTGGTGGATTGTGGAAAAAAATTATTTAATTAGACCGCTTATCGGTGCGTTTGCTCTGGCAATGTCT
TCCAGCTTAGGGAGTTTTGCGGTTATTGCTTTTTTCGGAAGCCCGGATTTTAGCTCGTTACCTTATTTGTTATATCAACA
ACTTGGCAGTTATCGAACCGAAGATGCGGCGGTGACTGCTTTAGTATTGATGCTATTCACATTATTGCCGTTTTTATTTA
TAGCACAGAAAGAGAAAATCAAATGA

Upstream 100 bases:

>100_bases
ATATACGCGTATTAATTAATCAAAAGGAGTGAATTTTGCCTCACGTCAATTTCGGGAAATAAGCGCTAATTAAACGATTG
CGTAAAAAATAGTTGAGCAT

Downstream 100 bases:

>100_bases
TTCGCTTGAATGTCCGTTTTGATTATGAACAAATCCCAATGCATTTTGTGCTAGGAGTACCAAAGGGACAACGTGTTGCG
ATTGTTGGGGAAAGCGGCGC

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MLKFIHSFTIKENFSRFLARYSAWSVYGIIVAIYAMSLFALMAHRQDDSMWSVSDVLPILKYSFLQAGLSAVLTCMLGVL
LARAFFYLDFIGKSYLYKILSFVWALPSLVVIFALIGFWGNSGWIAAFLQWLGIEWQFQLYGLQGILLAHVFFNVPLVTK
YCVESLTLIPSSQHRLAAQMNLKGWSYFKIVELPMLSGTLPYAFANVFLICFTSFPIVLMLGGSPKYSTLEVAIYQAVTF
EFDFAKAVILIMVQLAVGILLQLLMDFTTKYAFNNAKQYTPVVDMIWKPNPMGWQKKGLQIVLLLQSLLIILPLTSVVWA
GISVSNFSERLLNSALWEAAWFSFLLSLIASCSVLFIAYLLALETRQLAFKHQKWQYGILNGVATYPLILPVFLLAVGLF
LLLMDVELSTLHLLWLVGICNGLTLLPYIYRLIFAAMWHSLTAQDKLARSLGLSGFRRWWIVEKNYLIRPLIGAFALAMS
SSLGSFAVIAFFGSPDFSSLPYLLYQQLGSYRTEDAAVTALVLMLFTLLPFLFIAQKEKIK

Sequences:

>Translated_541_residues
MLKFIHSFTIKENFSRFLARYSAWSVYGIIVAIYAMSLFALMAHRQDDSMWSVSDVLPILKYSFLQAGLSAVLTCMLGVL
LARAFFYLDFIGKSYLYKILSFVWALPSLVVIFALIGFWGNSGWIAAFLQWLGIEWQFQLYGLQGILLAHVFFNVPLVTK
YCVESLTLIPSSQHRLAAQMNLKGWSYFKIVELPMLSGTLPYAFANVFLICFTSFPIVLMLGGSPKYSTLEVAIYQAVTF
EFDFAKAVILIMVQLAVGILLQLLMDFTTKYAFNNAKQYTPVVDMIWKPNPMGWQKKGLQIVLLLQSLLIILPLTSVVWA
GISVSNFSERLLNSALWEAAWFSFLLSLIASCSVLFIAYLLALETRQLAFKHQKWQYGILNGVATYPLILPVFLLAVGLF
LLLMDVELSTLHLLWLVGICNGLTLLPYIYRLIFAAMWHSLTAQDKLARSLGLSGFRRWWIVEKNYLIRPLIGAFALAMS
SSLGSFAVIAFFGSPDFSSLPYLLYQQLGSYRTEDAAVTALVLMLFTLLPFLFIAQKEKIK
>Mature_541_residues
MLKFIHSFTIKENFSRFLARYSAWSVYGIIVAIYAMSLFALMAHRQDDSMWSVSDVLPILKYSFLQAGLSAVLTCMLGVL
LARAFFYLDFIGKSYLYKILSFVWALPSLVVIFALIGFWGNSGWIAAFLQWLGIEWQFQLYGLQGILLAHVFFNVPLVTK
YCVESLTLIPSSQHRLAAQMNLKGWSYFKIVELPMLSGTLPYAFANVFLICFTSFPIVLMLGGSPKYSTLEVAIYQAVTF
EFDFAKAVILIMVQLAVGILLQLLMDFTTKYAFNNAKQYTPVVDMIWKPNPMGWQKKGLQIVLLLQSLLIILPLTSVVWA
GISVSNFSERLLNSALWEAAWFSFLLSLIASCSVLFIAYLLALETRQLAFKHQKWQYGILNGVATYPLILPVFLLAVGLF
LLLMDVELSTLHLLWLVGICNGLTLLPYIYRLIFAAMWHSLTAQDKLARSLGLSGFRRWWIVEKNYLIRPLIGAFALAMS
SSLGSFAVIAFFGSPDFSSLPYLLYQQLGSYRTEDAAVTALVLMLFTLLPFLFIAQKEKIK

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=479, Percent_Identity=35.2818371607516, Blast_Score=268, Evalue=7e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 61181; Mature: 61181

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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C
>Mature Secondary Structure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C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]