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The map label for this gene is hybF [H]

Identifier: 126208802

GI number: 126208802

Start: 1534183

End: 1534524

Strand: Reverse

Name: hybF [H]

Synonym: APL_1338

Alternate gene names: 126208802

Gene position: 1534524-1534183 (Counterclockwise)

Preceding gene: 126208803

Following gene: 126208794

Centisome position: 67.47

GC content: 40.94

Gene sequence:

>342_bases
ATGCACGAGATGTCTTTATGCCAAAGTATTCTTCAAATCATAGAGAAAGAATGTCAGCCTCAAAACGTTAAACAGGTAAC
CGATTTATGGCTGGAAATCGGCGCTTTATCTTGTGTGGAAAAATCGTCACTGGAATTTTGTTTTAATACCGTTTGTCGAG
ATACGATAGCGGAAGGCTGTCAATTACACTTTATCGATATACCTGCAAAAGCGTGGTGCTGGGGCTGTAGTCTATCGGTC
GAGTTAGCCGTAGCCCAAACGATATGTCCTCATTGCGGTTCTCAGGATTTGCATATTCAACAAGGCAGCGAATTAAAAAT
AAAAGAACTGGCGGTAAAATAA

Upstream 100 bases:

>100_bases
TCTTAAGTATCTTCGTATTAACTTATTTATATTATAATTAAGCAAAATTTATATCGTTTTTTCTAACGACATTTGTAATT
TAATAAAATAAGGAATGGTT

Downstream 100 bases:

>100_bases
GAGTATTTTCTTGATTGGTATAAGAAAGCCGAATAACGTTGTGTTATAAACACTTTTTATTCGGCTTTTTTTCTATTTAT
TTTTAGCTATCTCACTAAGA

Product: putative hydrogenase nickel incorporation protein HybF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 113; Mature: 113

Protein sequence:

>113_residues
MHEMSLCQSILQIIEKECQPQNVKQVTDLWLEIGALSCVEKSSLEFCFNTVCRDTIAEGCQLHFIDIPAKAWCWGCSLSV
ELAVAQTICPHCGSQDLHIQQGSELKIKELAVK

Sequences:

>Translated_113_residues
MHEMSLCQSILQIIEKECQPQNVKQVTDLWLEIGALSCVEKSSLEFCFNTVCRDTIAEGCQLHFIDIPAKAWCWGCSLSV
ELAVAQTICPHCGSQDLHIQQGSELKIKELAVK
>Mature_113_residues
MHEMSLCQSILQIIEKECQPQNVKQVTDLWLEIGALSCVEKSSLEFCFNTVCRDTIAEGCQLHFIDIPAKAWCWGCSLSV
ELAVAQTICPHCGSQDLHIQQGSELKIKELAVK

Specific function: Probably plays a role in an hydrogenase nickel cofactor insertion step [H]

COG id: COG0375

COG function: function code R; Zn finger protein HypA/HybF (possibly regulating hydrogenase expression)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypA/hybF family [H]

Homologues:

Organism=Escherichia coli, GI1789365, Length=113, Percent_Identity=47.787610619469, Blast_Score=117, Evalue=2e-28,
Organism=Escherichia coli, GI1789081, Length=113, Percent_Identity=43.3628318584071, Blast_Score=98, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000688
- InterPro:   IPR020538 [H]

Pfam domain/function: PF01155 HypA [H]

EC number: NA

Molecular weight: Translated: 12621; Mature: 12621

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS01249 HYPA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
10.6 %Cys+Met (Translated Protein)
8.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
10.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEMSLCQSILQIIEKECQPQNVKQVTDLWLEIGALSCVEKSSLEFCFNTVCRDTIAEGC
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QLHFIDIPAKAWCWGCSLSVELAVAQTICPHCGSQDLHIQQGSELKIKELAVK
EEEEEECCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHCCC
>Mature Secondary Structure
MHEMSLCQSILQIIEKECQPQNVKQVTDLWLEIGALSCVEKSSLEFCFNTVCRDTIAEGC
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QLHFIDIPAKAWCWGCSLSVELAVAQTICPHCGSQDLHIQQGSELKIKELAVK
EEEEEECCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3308458 [H]