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The map label for this gene is metK [H]

Identifier: 126208552

GI number: 126208552

Start: 1249095

End: 1250246

Strand: Reverse

Name: metK [H]

Synonym: APL_1080

Alternate gene names: 126208552

Gene position: 1250246-1249095 (Counterclockwise)

Preceding gene: 126208558

Following gene: 126208551

Centisome position: 54.97

GC content: 44.79

Gene sequence:

>1152_bases
ATGGCGATTAATTTATTTACTTCAGAATCGGTATCGGAAGGACATCCGGATAAAATTGCGGATCAAATCTCGGATGCGGT
GTTAGATGAAATTTTAAAACAAGACCCGAAAGCACGTGTGGCGTGTGAAACTTATGTAAAAACCGGTATGGCGTTAGTCG
GCGGTGAAATCACCACTTCTGCATGGGTGGATATCGAAAACTTAACCCGTCAAGTGATTTGCGACATCGGCTATACCCAT
TCTGATATGGGCTTTGATGCGCATTCTTGTGCGGTATTAAATGCGATTGGTAAACAATCTCCGGATATTAACCAAGGTGT
TGACCGTGCGGATCCGTTAGAACAAGGTGCGGGCGACCAAGGTATTATGTTCGGTTATGCAACCAATGAAACCGATGTAT
TAATGCCGGCACCGATTACATATGCTCATCGTTTAATGGAACAACAAGCAAAAGTACGTAAATCTGGCAAATTAGACTGG
TTACGTCCGGATGCGAAAAGCCAATTAACTTTCGCTTACGAAAATAACAAAATCGTAGGGATTGATGCGGTGGTGCTTTC
AACTCAACATGCTGAACACGTTTCACAAAAAGATTTAGTGGAAGGTGTGATGGAAGAAATCATCAAGCCGGTATTGCCAA
GCGAATGGTTAAGCCAAAACACCAAATATTTTATTAACCCGACCGGTCGTTTCGTGATTGGCGGCCCAATGGGTGACTGT
GGTTTAACAGGCCGTAAAATTATCGTAGATACTTACGGCGGTGCGGCTCGGCACGGTGGCGGTGCATTCTCCGGTAAAGA
CCCGTCAAAAGTGGACCGTTCTGCGGCATACGCAGCACGTTATGTGGCGAAAAATATCGTGGCGGCAGGTTTAGCGGATC
GTTGTGAAATCCAACTTTCTTATGCAATCGGTGTGGCGGATCCGACATCGATTATGGTGGAAACGTTTGGCACGGGTAGA
GTTTCAAACGAAACATTAGTGAAATTGATCTATCAAAACTTTGATTTACGTCCGTACGGCTTAATCAAAATGTTAGATTT
AATTCAACCTATTTATCGTGAAACGGCGGCTTACGGCCACTTCGGCCGTGAACACTTCCCATGGGAACAAACGGATAAAG
CGGAAGCATTACGTGCCGGTGCAGGTTTATAA

Upstream 100 bases:

>100_bases
CTAATTTTGCAAATAGTTTGTGATTTTTGACCGCTTGTAAATTTGATTGAGGGAGCAAAAGCTTCAACTATGATAAAGGT
ATAACCAAAGGATAATAACA

Downstream 100 bases:

>100_bases
TGTTAAGTTGATATAGTTTAACTAATATAAAGCGGTTGAATTTACAGGGAAATTTGTAAATTCAACCGCTTATTTTTTAA
CTGTTCATTGTGGCGCAAAC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MAINLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTH
SDMGFDAHSCAVLNAIGKQSPDINQGVDRADPLEQGAGDQGIMFGYATNETDVLMPAPITYAHRLMEQQAKVRKSGKLDW
LRPDAKSQLTFAYENNKIVGIDAVVLSTQHAEHVSQKDLVEGVMEEIIKPVLPSEWLSQNTKYFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGR
VSNETLVKLIYQNFDLRPYGLIKMLDLIQPIYRETAAYGHFGREHFPWEQTDKAEALRAGAGL

Sequences:

>Translated_383_residues
MAINLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTH
SDMGFDAHSCAVLNAIGKQSPDINQGVDRADPLEQGAGDQGIMFGYATNETDVLMPAPITYAHRLMEQQAKVRKSGKLDW
LRPDAKSQLTFAYENNKIVGIDAVVLSTQHAEHVSQKDLVEGVMEEIIKPVLPSEWLSQNTKYFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGR
VSNETLVKLIYQNFDLRPYGLIKMLDLIQPIYRETAAYGHFGREHFPWEQTDKAEALRAGAGL
>Mature_382_residues
AINLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTHS
DMGFDAHSCAVLNAIGKQSPDINQGVDRADPLEQGAGDQGIMFGYATNETDVLMPAPITYAHRLMEQQAKVRKSGKLDWL
RPDAKSQLTFAYENNKIVGIDAVVLSTQHAEHVSQKDLVEGVMEEIIKPVLPSEWLSQNTKYFINPTGRFVIGGPMGDCG
LTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGRV
SNETLVKLIYQNFDLRPYGLIKMLDLIQPIYRETAAYGHFGREHFPWEQTDKAEALRAGAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=373, Percent_Identity=55.7640750670241, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI5174529, Length=371, Percent_Identity=55.5256064690027, Blast_Score=399, Evalue=1e-111,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=83.289817232376, Blast_Score=675, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17538498, Length=371, Percent_Identity=57.1428571428571, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17551082, Length=371, Percent_Identity=55.7951482479784, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17538494, Length=371, Percent_Identity=56.6037735849057, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25145633, Length=374, Percent_Identity=56.4171122994652, Blast_Score=403, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI32565905, Length=371, Percent_Identity=50.6738544474393, Blast_Score=343, Evalue=8e-95,
Organism=Caenorhabditis elegans, GI32565911, Length=326, Percent_Identity=53.9877300613497, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI25145635, Length=329, Percent_Identity=53.1914893617021, Blast_Score=331, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI32565903, Length=302, Percent_Identity=52.9801324503311, Blast_Score=303, Evalue=8e-83,
Organism=Caenorhabditis elegans, GI32565909, Length=81, Percent_Identity=65.4320987654321, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=72.7272727272727, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=376, Percent_Identity=57.4468085106383, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6323209, Length=371, Percent_Identity=57.6819407008086, Blast_Score=409, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45552159, Length=380, Percent_Identity=55.5263157894737, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580529, Length=380, Percent_Identity=55.5263157894737, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580537, Length=380, Percent_Identity=55.5263157894737, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580533, Length=380, Percent_Identity=55.5263157894737, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580535, Length=380, Percent_Identity=55.5263157894737, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580531, Length=380, Percent_Identity=55.5263157894737, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580527, Length=379, Percent_Identity=54.6174142480211, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580525, Length=379, Percent_Identity=54.6174142480211, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580541, Length=378, Percent_Identity=52.3809523809524, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24580539, Length=117, Percent_Identity=56.4102564102564, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41689; Mature: 41558

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAINLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTS
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHCCCCCCHH
AWVDIENLTRQVICDIGYTHSDMGFDAHSCAVLNAIGKQSPDINQGVDRADPLEQGAGDQ
HEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHCCCCCCCHHHHCCCCC
GIMFGYATNETDVLMPAPITYAHRLMEQQAKVRKSGKLDWLRPDAKSQLTFAYENNKIVG
CEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEECCEEEE
IDAVVLSTQHAEHVSQKDLVEGVMEEIIKPVLPSEWLSQNTKYFINPTGRFVIGGPMGDC
EEEEEEECHHHHHCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVADPTSIMVETFGTGRVSNETLVKLIYQNFDLRPYGLIKMLDLIQPIYRETAAYGH
EEEECCCCHHEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
FGREHFPWEQTDKAEALRAGAGL
CCCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
AINLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTS
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHCCCCCCHH
AWVDIENLTRQVICDIGYTHSDMGFDAHSCAVLNAIGKQSPDINQGVDRADPLEQGAGDQ
HEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHCCCCCCCHHHHCCCCC
GIMFGYATNETDVLMPAPITYAHRLMEQQAKVRKSGKLDWLRPDAKSQLTFAYENNKIVG
CEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEECCEEEE
IDAVVLSTQHAEHVSQKDLVEGVMEEIIKPVLPSEWLSQNTKYFINPTGRFVIGGPMGDC
EEEEEEECHHHHHCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVADPTSIMVETFGTGRVSNETLVKLIYQNFDLRPYGLIKMLDLIQPIYRETAAYGH
EEEECCCCHHEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
FGREHFPWEQTDKAEALRAGAGL
CCCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA