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The map label for this gene is pflA [H]

Identifier: 126208509

GI number: 126208509

Start: 1202068

End: 1202805

Strand: Reverse

Name: pflA [H]

Synonym: APL_1035

Alternate gene names: 126208509

Gene position: 1202805-1202068 (Counterclockwise)

Preceding gene: 126208510

Following gene: 126208508

Centisome position: 52.88

GC content: 42.95

Gene sequence:

>738_bases
ATGTCTATTGCTCGTTACCACTCATACGAATCTTGCGGCACGGTTGATGGTCCGGGAATCCGCTTTATTCTGTTTTTACA
AGGCTGTTTGATGCGTTGCAAATACTGCCATAACCGTGATACGTGGGATCTTGACGGCGGTAAAGAAATCAGTGTCGAAG
ATCTGATGAAAGAAGTGGTAACCTACAAGCATTTTATGAAAGCGACCGGCGGCGGTGTAACTGCATCCGGCGGCGAAGCC
GTATTGCAGATGGAATTTGTAAGAGATTGGTTTAGAGCCTGTAAAGCGGAAGGCATTGATACTTGTTTAGATACCAACGG
ATTCGTACGCCATTATAGCCCGGTCGTTGATGAAATGCTCGAAGTGACCGATTTAGTGATGCTCGACTTAAAGCAGCTAA
ATGATGAAATCCATCAAGATCTAATCGGTGTATCAAATAAACGTACGCTTGATTTTGCTCGCTATCTGCAAAAATTAAAT
AAACGTACTTGGATTCGTTATGTTGTCGTACCTGGTTATACCGATGACGATGATTCCGCACACCGCCTAGGGCAATTTAT
CCAGGGTATGCAAAATATTGAGAAAGTCGAGTTATTGCCATATCACCGCTTGGGCGCACATAAATGGGAAACACTCGGTT
ATAAATATGAATTGGAAGGCGTATTACCGCCGCCGAAAGAAACAATGGATCGTTTAAAAGCGATTATTGAAAGTTACGGT
CATACCGTAAAATACTAA

Upstream 100 bases:

>100_bases
TCGGATTTTGCAAAAATTTTGCGGAATCCGACCGCTTCTTTATTGATATTCTGCTAAAATAATAACAACGAATTTATACT
TACTATCAAAGGAATCATAT

Downstream 100 bases:

>100_bases
TCGTACAAGCGGTCGAATTTAATCTGTTTTTTGTAAAAAATAGGCTGAATTCGACCGCTTTTTACTTGAAAGACTTGATC
TTTTCCTTCCTTAACCCAAT

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSIARYHSYESCGTVDGPGIRFILFLQGCLMRCKYCHNRDTWDLDGGKEISVEDLMKEVVTYKHFMKATGGGVTASGGEA
VLQMEFVRDWFRACKAEGIDTCLDTNGFVRHYSPVVDEMLEVTDLVMLDLKQLNDEIHQDLIGVSNKRTLDFARYLQKLN
KRTWIRYVVVPGYTDDDDSAHRLGQFIQGMQNIEKVELLPYHRLGAHKWETLGYKYELEGVLPPPKETMDRLKAIIESYG
HTVKY

Sequences:

>Translated_245_residues
MSIARYHSYESCGTVDGPGIRFILFLQGCLMRCKYCHNRDTWDLDGGKEISVEDLMKEVVTYKHFMKATGGGVTASGGEA
VLQMEFVRDWFRACKAEGIDTCLDTNGFVRHYSPVVDEMLEVTDLVMLDLKQLNDEIHQDLIGVSNKRTLDFARYLQKLN
KRTWIRYVVVPGYTDDDDSAHRLGQFIQGMQNIEKVELLPYHRLGAHKWETLGYKYELEGVLPPPKETMDRLKAIIESYG
HTVKY
>Mature_244_residues
SIARYHSYESCGTVDGPGIRFILFLQGCLMRCKYCHNRDTWDLDGGKEISVEDLMKEVVTYKHFMKATGGGVTASGGEAV
LQMEFVRDWFRACKAEGIDTCLDTNGFVRHYSPVVDEMLEVTDLVMLDLKQLNDEIHQDLIGVSNKRTLDFARYLQKLNK
RTWIRYVVVPGYTDDDDSAHRLGQFIQGMQNIEKVELLPYHRLGAHKWETLGYKYELEGVLPPPKETMDRLKAIIESYGH
TVKY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=243, Percent_Identity=74.8971193415638, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1790389, Length=271, Percent_Identity=24.3542435424354, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1790839, Length=251, Percent_Identity=29.8804780876494, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI226510931, Length=173, Percent_Identity=30.0578034682081, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28170; Mature: 28038

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00018 EF_HAND_1 ; PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCC
>Mature Secondary Structure 
SIARYHSYESCGTVDGPGIRFILFLQGCLMRCKYCHNRDTWDLDGGKEISVEDLMKEVV
CCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHHHHHHH
TYKHFMKATGGGVTASGGEAVLQMEFVRDWFRACKAEGIDTCLDTNGFVRHYSPVVDEML
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHH
EVTDLVMLDLKQLNDEIHQDLIGVSNKRTLDFARYLQKLNKRTWIRYVVVPGYTDDDDSA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHH
HRLGQFIQGMQNIEKVELLPYHRLGAHKWETLGYKYELEGVLPPPKETMDRLKAIIESYG
HHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHC
HTVKY
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]