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The map label for this gene is pflB [H]

Identifier: 126208510

GI number: 126208510

Start: 1202932

End: 1205244

Strand: Reverse

Name: pflB [H]

Synonym: APL_1036

Alternate gene names: 126208510

Gene position: 1205244-1202932 (Counterclockwise)

Preceding gene: 126208511

Following gene: 126208509

Centisome position: 52.99

GC content: 42.67

Gene sequence:

>2313_bases
ATGACTCAATTAACTGAAGCTCAACAAAAAGCATGGGCAGGGTTTACTGGTGGCGACTGGCAAACTGAAGTAAACGTACG
TGATTTTATCCAAAAAAACTATACTCCGTATGAAGGCGACGAGTCTTTCTTAGCTGGCGCAACCGCTGCGACAACTAAGT
TATGGGAAGAAGTGATGGAAAAAATTAAAGTTGAGAACAAAACTCACGAACCGTACGATATTGATTGCGACATTCCATCA
ACGATTACTTCTCACAAAGCGGGTTATATCGATCAAGCTTTAGAGAAAATCGTAGGTCTTCAAACTGATGCGCCGTTAAA
ACGTGCGATTATCCCGTTCGGCGGTATCAAAATGGTTAAAGGTTCTTGCGATGTTTATCGCCGTACCTTAAACCCTGAAG
TAGAAAAAATCTTTACCGAATATCGTAAAACACACAACCAAGGTGTGTTCGATGTTTATACGCCGGATATTTTACGTTGC
CGTAAATCAGGTGTAATTACCGGTCTTCCGGATGCTTACGGTCGTGGTCGTATTATCGGTGACTACCGTCGTTTAGCCGT
TTACGGTGCGGACTTCTTAATGAAAGACAAGCAAAAACAATTTGCTTCATTACAACCTCGTTTAGAAGCGGGTGAAGATA
TCCAAGCAACAATTCAATTACGTGAAGAAATCGCAGAACAACACCGTGCATTAGGTCAAATCAAACAAATGGCCGCATCA
TACGGTTTTGACGTTTCTCGTCCGGCGGAAACCGCACAAGAAGCGGTTCAATGGACTTACTTCGCATACCTTGCTGCGGT
TAAATCACAAAACGGTGCGGCAATGTCATTCGGTCGTGTATCTTCATTCTTAGATATCTATATCGAACGTGACTTAAAAG
CAGGTAAAATCACAGAAGAAGAAGCACAAGAGTTAATCGACCATTTAGTAATGAAATTACGTATGGTTCGTTTCTTACGT
ACACCTGAATACGATCAATTATTCTCAGGCGACCCAATGTGGGCAACCGAGACTTTAGCAGGTATGGGGTTAGACGGTCG
TACATTAGTAACCAAAAACAGCTTCCGTATCTTAAATACCCTTTACACAATGGGTCCGTCACCGGAACCGAACTTAACTA
TTCTTTGGTCTGAACAATTACCGGACGGCTTCAAACGTTATTGTGCGAAAGTATCTATCGATACTTCATCAGTACAATAC
GAAAACGATGACTTAATGCGTCCTGACTTCGATAACGATGACTATGCAATCGCATGTTGCGTATCTCCGATGGTTGTCGG
TAAACAAATGCAATTCTTCGGTGCACGTGCAAACTTAGCGAAAACAATGTTATACGCAATCAACGGCGGTATCGATGAAA
AATCCGGCGCACAAGTCGGTCCGAAAACAGCACCGATTACAGACGAATACTTAAACTTCGACGATGTAATGGCTCGTATG
GATCACTTCATGGATTGGTTAGCGACACAATATGTGACCGCATTAAATATCATCCACTTCATGCACGATAAATACAGCTA
TGAAGCAGCATTAATGGCATTCCACGATCGTGATGTATTCCGTACAATGGCGTGCGGTATCGCAGGTCTTTCCGTTGCGG
CTGACTCATTATCTGCAATTAAATACGCAAAAGTGAAACCTGTTCGTGGTGACATCAAAGATAAAGACGGTAACGTAGTC
GCTACAAACGTCGCGTTAGACTTCGAAATCGAAGGCGAATATCCGCAATTCGGTAACAATGACAACCGTGTAGATGAAAT
CGCTTGTGACTTAGTTGAACGTTTCATGAAAAAAATCCAAACGCACAAAACTTACCGCAATGCGACACCAACACAGTCAG
TACTTACTATCACTTCTAACGTGGTTTACGGTAAAAAAACTGGTAATACGCCTGATGGTCGTCGTGCTGGTGCACCGTTT
GGCCCGGGTGCAAACCCAATGCACGGTCGTGACCAAAAAGGTGCGGTTGCGTCATTAACTTCTGTTGCAAAACTTCCGTT
TGCGTATGCGAAAGACGGTATTTCATATACCTTCTCAATCGTACCAAACGCATTAGGTAAAGATTACGAAGCACAAAAAC
GTAACCTTGCAGGTCTAATGGACGGTTACTTCCACCACGAAGCAACTGTTGAAGGCGGTCAGCACTTAAACGTAAACGTA
TTAAACCGTGAAACATTATTAGATGCGGTTGAGCATCCGGAAAAATATCCGCAATTAACCATTCGTGTATCCGGATATGC
GGTACGTTTCAACTCTTTAACTAAAGAGCAACAAATGGACGTAATTACTCGTACATTTACCGAATCAATGTAA

Upstream 100 bases:

>100_bases
AGCACTAACATAATTTCAATTTAAGCGGTTTTATTTGGGGAGTTTTTTGCAAAAATGACCGCTTGTACAGCTTAATTTAC
TAACAAATAGAGGACTTAAA

Downstream 100 bases:

>100_bases
TCCCTCACATTGACCTTTTAAAGCGGTCGGATTTTGCAAAAATTTTGCGGAATCCGACCGCTTCTTTATTGATATTCTGC
TAAAATAATAACAACGAATT

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 770; Mature: 769

Protein sequence:

>770_residues
MTQLTEAQQKAWAGFTGGDWQTEVNVRDFIQKNYTPYEGDESFLAGATAATTKLWEEVMEKIKVENKTHEPYDIDCDIPS
TITSHKAGYIDQALEKIVGLQTDAPLKRAIIPFGGIKMVKGSCDVYRRTLNPEVEKIFTEYRKTHNQGVFDVYTPDILRC
RKSGVITGLPDAYGRGRIIGDYRRLAVYGADFLMKDKQKQFASLQPRLEAGEDIQATIQLREEIAEQHRALGQIKQMAAS
YGFDVSRPAETAQEAVQWTYFAYLAAVKSQNGAAMSFGRVSSFLDIYIERDLKAGKITEEEAQELIDHLVMKLRMVRFLR
TPEYDQLFSGDPMWATETLAGMGLDGRTLVTKNSFRILNTLYTMGPSPEPNLTILWSEQLPDGFKRYCAKVSIDTSSVQY
ENDDLMRPDFDNDDYAIACCVSPMVVGKQMQFFGARANLAKTMLYAINGGIDEKSGAQVGPKTAPITDEYLNFDDVMARM
DHFMDWLATQYVTALNIIHFMHDKYSYEAALMAFHDRDVFRTMACGIAGLSVAADSLSAIKYAKVKPVRGDIKDKDGNVV
ATNVALDFEIEGEYPQFGNNDNRVDEIACDLVERFMKKIQTHKTYRNATPTQSVLTITSNVVYGKKTGNTPDGRRAGAPF
GPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDYEAQKRNLAGLMDGYFHHEATVEGGQHLNVNV
LNRETLLDAVEHPEKYPQLTIRVSGYAVRFNSLTKEQQMDVITRTFTESM

Sequences:

>Translated_770_residues
MTQLTEAQQKAWAGFTGGDWQTEVNVRDFIQKNYTPYEGDESFLAGATAATTKLWEEVMEKIKVENKTHEPYDIDCDIPS
TITSHKAGYIDQALEKIVGLQTDAPLKRAIIPFGGIKMVKGSCDVYRRTLNPEVEKIFTEYRKTHNQGVFDVYTPDILRC
RKSGVITGLPDAYGRGRIIGDYRRLAVYGADFLMKDKQKQFASLQPRLEAGEDIQATIQLREEIAEQHRALGQIKQMAAS
YGFDVSRPAETAQEAVQWTYFAYLAAVKSQNGAAMSFGRVSSFLDIYIERDLKAGKITEEEAQELIDHLVMKLRMVRFLR
TPEYDQLFSGDPMWATETLAGMGLDGRTLVTKNSFRILNTLYTMGPSPEPNLTILWSEQLPDGFKRYCAKVSIDTSSVQY
ENDDLMRPDFDNDDYAIACCVSPMVVGKQMQFFGARANLAKTMLYAINGGIDEKSGAQVGPKTAPITDEYLNFDDVMARM
DHFMDWLATQYVTALNIIHFMHDKYSYEAALMAFHDRDVFRTMACGIAGLSVAADSLSAIKYAKVKPVRGDIKDKDGNVV
ATNVALDFEIEGEYPQFGNNDNRVDEIACDLVERFMKKIQTHKTYRNATPTQSVLTITSNVVYGKKTGNTPDGRRAGAPF
GPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDYEAQKRNLAGLMDGYFHHEATVEGGQHLNVNV
LNRETLLDAVEHPEKYPQLTIRVSGYAVRFNSLTKEQQMDVITRTFTESM
>Mature_769_residues
TQLTEAQQKAWAGFTGGDWQTEVNVRDFIQKNYTPYEGDESFLAGATAATTKLWEEVMEKIKVENKTHEPYDIDCDIPST
ITSHKAGYIDQALEKIVGLQTDAPLKRAIIPFGGIKMVKGSCDVYRRTLNPEVEKIFTEYRKTHNQGVFDVYTPDILRCR
KSGVITGLPDAYGRGRIIGDYRRLAVYGADFLMKDKQKQFASLQPRLEAGEDIQATIQLREEIAEQHRALGQIKQMAASY
GFDVSRPAETAQEAVQWTYFAYLAAVKSQNGAAMSFGRVSSFLDIYIERDLKAGKITEEEAQELIDHLVMKLRMVRFLRT
PEYDQLFSGDPMWATETLAGMGLDGRTLVTKNSFRILNTLYTMGPSPEPNLTILWSEQLPDGFKRYCAKVSIDTSSVQYE
NDDLMRPDFDNDDYAIACCVSPMVVGKQMQFFGARANLAKTMLYAINGGIDEKSGAQVGPKTAPITDEYLNFDDVMARMD
HFMDWLATQYVTALNIIHFMHDKYSYEAALMAFHDRDVFRTMACGIAGLSVAADSLSAIKYAKVKPVRGDIKDKDGNVVA
TNVALDFEIEGEYPQFGNNDNRVDEIACDLVERFMKKIQTHKTYRNATPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFG
PGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDYEAQKRNLAGLMDGYFHHEATVEGGQHLNVNVL
NRETLLDAVEHPEKYPQLTIRVSGYAVRFNSLTKEQQMDVITRTFTESM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=770, Percent_Identity=81.2987012987013, Blast_Score=1337, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=760, Percent_Identity=75.3947368421053, Blast_Score=1238, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=582, Percent_Identity=28.0068728522337, Blast_Score=197, Evalue=3e-51,
Organism=Escherichia coli, GI1790388, Length=639, Percent_Identity=26.9170579029734, Blast_Score=156, Evalue=5e-39,
Organism=Escherichia coli, GI1788933, Length=64, Percent_Identity=85.9375, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 86175; Mature: 86043

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLTEAQQKAWAGFTGGDWQTEVNVRDFIQKNYTPYEGDESFLAGATAATTKLWEEVME
CCCCHHHHHHHHCCCCCCCCEEECCHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHH
KIKVENKTHEPYDIDCDIPSTITSHKAGYIDQALEKIVGLQTDAPLKRAIIPFGGIKMVK
HHHCCCCCCCCCEECCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEC
GSCDVYRRTLNPEVEKIFTEYRKTHNQGVFDVYTPDILRCRKSGVITGLPDAYGRGRIIG
CCHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCEECCCCCCCCCCEEH
DYRRLAVYGADFLMKDKQKQFASLQPRLEAGEDIQATIQLREEIAEQHRALGQIKQMAAS
HHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGFDVSRPAETAQEAVQWTYFAYLAAVKSQNGAAMSFGRVSSFLDIYIERDLKAGKITEE
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHH
EAQELIDHLVMKLRMVRFLRTPEYDQLFSGDPMWATETLAGMGLDGRTLVTKNSFRILNT
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHCCCCCCCEEEECCCHHHHHH
LYTMGPSPEPNLTILWSEQLPDGFKRYCAKVSIDTSSVQYENDDLMRPDFDNDDYAIACC
HHHCCCCCCCCEEEEECCCCCHHHHHHHHEEEECCCCEEECCCCCCCCCCCCCCCEEEEE
VSPMVVGKQMQFFGARANLAKTMLYAINGGIDEKSGAQVGPKTAPITDEYLNFDDVMARM
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHH
DHFMDWLATQYVTALNIIHFMHDKYSYEAALMAFHDRDVFRTMACGIAGLSVAADSLSAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYAKVKPVRGDIKDKDGNVVATNVALDFEIEGEYPQFGNNDNRVDEIACDLVERFMKKIQ
HHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
THKTYRNATPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLT
HHHHHCCCCCCHHEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
SVAKLPFAYAKDGISYTFSIVPNALGKDYEAQKRNLAGLMDGYFHHEATVEGGQHLNVNV
HHHHCCHHHHCCCCEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCHHEECCCCEEEEEE
LNRETLLDAVEHPEKYPQLTIRVSGYAVRFNSLTKEQQMDVITRTFTESM
ECHHHHHHHHHCCCCCCEEEEEEECEEEEECHHCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQLTEAQQKAWAGFTGGDWQTEVNVRDFIQKNYTPYEGDESFLAGATAATTKLWEEVME
CCCHHHHHHHHCCCCCCCCEEECCHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHH
KIKVENKTHEPYDIDCDIPSTITSHKAGYIDQALEKIVGLQTDAPLKRAIIPFGGIKMVK
HHHCCCCCCCCCEECCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEC
GSCDVYRRTLNPEVEKIFTEYRKTHNQGVFDVYTPDILRCRKSGVITGLPDAYGRGRIIG
CCHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCEECCCCCCCCCCEEH
DYRRLAVYGADFLMKDKQKQFASLQPRLEAGEDIQATIQLREEIAEQHRALGQIKQMAAS
HHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGFDVSRPAETAQEAVQWTYFAYLAAVKSQNGAAMSFGRVSSFLDIYIERDLKAGKITEE
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHH
EAQELIDHLVMKLRMVRFLRTPEYDQLFSGDPMWATETLAGMGLDGRTLVTKNSFRILNT
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHCCCCCCCEEEECCCHHHHHH
LYTMGPSPEPNLTILWSEQLPDGFKRYCAKVSIDTSSVQYENDDLMRPDFDNDDYAIACC
HHHCCCCCCCCEEEEECCCCCHHHHHHHHEEEECCCCEEECCCCCCCCCCCCCCCEEEEE
VSPMVVGKQMQFFGARANLAKTMLYAINGGIDEKSGAQVGPKTAPITDEYLNFDDVMARM
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHH
DHFMDWLATQYVTALNIIHFMHDKYSYEAALMAFHDRDVFRTMACGIAGLSVAADSLSAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYAKVKPVRGDIKDKDGNVVATNVALDFEIEGEYPQFGNNDNRVDEIACDLVERFMKKIQ
HHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
THKTYRNATPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLT
HHHHHCCCCCCHHEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
SVAKLPFAYAKDGISYTFSIVPNALGKDYEAQKRNLAGLMDGYFHHEATVEGGQHLNVNV
HHHHCCHHHHCCCCEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCHHEECCCCEEEEEE
LNRETLLDAVEHPEKYPQLTIRVSGYAVRFNSLTKEQQMDVITRTFTESM
ECHHHHHHHHHCCCCCCEEEEEEECEEEEECHHCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]