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The map label for this gene is purH [H]

Identifier: 126208434

GI number: 126208434

Start: 1100837

End: 1102426

Strand: Direct

Name: purH [H]

Synonym: APL_0958

Alternate gene names: 126208434

Gene position: 1100837-1102426 (Clockwise)

Preceding gene: 126208429

Following gene: 126208441

Centisome position: 48.4

GC content: 44.65

Gene sequence:

>1590_bases
ATGGCGATTCAACAGGCGTTATTAAGTGTGTCTGACAAAAAAGGTATTGTAGAATTTGCACAAGGGCTGGTAGCTCGCGG
TGTGAAATTACTTTCGACAGGCGGTACGGCAAAGCTGTTAGCTGAAGCTGGCTTACCGGTCACGGAAGTATCGGACTACA
CCGGCTTCCCTGAAATGATGGACGGTCGCGTAAAAACCCTGCATCCGAAAGTACACGGCGGTATTCTTGGTCGCCGCGGT
ACGGATGATGAAGTGATGAACCGACACGGTATCGAACGTATTGATATAGTGGTAGTAAACTTATACCCGTTTGCGGCAAC
CGTTGCGAATCCAAATTGCACCTTAGCGGATGCAGTTGAAAATATTGATATCGGCGGCCCGACCATGGTGCGTTCTGCAG
CGAAAAACCATAAAGATGTGGCGATCGTGGTGAATAATAACGATTTTGATGCAATTCTTGCCGAAATGGATCAAAACCAA
AACGAATTAACCCTTGAAACGCGTTTCAACCTGGCAATTAAAGCATTTGAACATACGGCACAATACGATTCAATGATTGC
AAACTACTTCGGTAAACTTGTCCCGCCATACCAAGGTGCTGAGGAAGAAGATTTAACTCAAGTCTGCGGTCAATTCCCTC
GTACATTAAACTTAAATTTCGTACGCAAACAAACCATGCGTTACGGTGAAAACTCACATCAAAATGCAGCATTCTATGTA
GAAAACGAAGTGAAAGAAGCTTCTGTTTCAACCGCAAAACAATTACAAGGTAAAGCACTTTCTTATAACAATATTGCGGA
TACCGATGCGGCACTTGAGTGCGTAAAAGAATTTGCAGAACCGGCTTGCGTGATTGTGAAACACGCTAACCCTTGCGGTG
TGGCATTAGGTAAAGACATTTTAGAAGCTTATAACCGTGCTTACCAAACCGACCCGACATCAGCATTCGGCGGTATTATT
GCTTTCAACCGTGAGTTAGACGGTGAAACGGCACAAACGATCGCAGAACGCCAATTTGTGGAAGTGATTATTGCTCCAAG
CGTTTCAGAAGCGGCAAAAGCAGCGCTTGCGAAGAAGAAAAATGTGCGTGTCTTAGAATGTGGTGAATTTACTCAAGCGC
AAAAACGTTTAGATTTCAAACGTGTAAACGGCGGTTTATTAGTGCAAGATGCGGATCAAGGTTCGGTTTCAATTGATGAT
TTACAAGTGGTATCCGAACGTAAACCAAGCAAAGAAGAGCTTGAAGACTTATTATTCTGCTGGAAAGTGGCAAAATATGT
GAAATCAAATGCGATTGTCTATGCGAAAAACGGTCAAACTATCGGTATCGGTGCAGGTCAAATGAGCCGTGTCTATTCAG
CGAAAATTGCCGGTATTAAAGCGGAAGACGAAGGCTTACAAGTTGCTGGCTGCGTAATGGCTTCAGATGCGTTCTTCCCA
TTCCGTGACGGTATTGATGCGGCAGCTAAAGTCGGCATTACTTGCGTAATCCATCCGGGTGGCTCAATGCGAGATCAAGA
AGTGATTGATGCTGCTAACGAACACGGTATGGCAATGGTCTTAACCGGTATGCGCCATTTCCGTCACTAA

Upstream 100 bases:

>100_bases
AAAACTTTGCAAGTTCGACCGCTTGCTAATGAAAAAAGCAAACGTTTGCGTTATAATAGAAAAGTATTTATTTAACATTC
ATTTTAAGGAACTTAGAAAA

Downstream 100 bases:

>100_bases
TTATGTCCGAACAAGCGGTCTATTTTCACTAAAACTTTGTAAACTTTCCTCCTCCGTTCTAATTGAATTGTAAAAAATAA
AAGAAAATAGACCGCTTTGT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MAIQQALLSVSDKKGIVEFAQGLVARGVKLLSTGGTAKLLAEAGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRG
TDDEVMNRHGIERIDIVVVNLYPFAATVANPNCTLADAVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFDAILAEMDQNQ
NELTLETRFNLAIKAFEHTAQYDSMIANYFGKLVPPYQGAEEEDLTQVCGQFPRTLNLNFVRKQTMRYGENSHQNAAFYV
ENEVKEASVSTAKQLQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVALGKDILEAYNRAYQTDPTSAFGGII
AFNRELDGETAQTIAERQFVEVIIAPSVSEAAKAALAKKKNVRVLECGEFTQAQKRLDFKRVNGGLLVQDADQGSVSIDD
LQVVSERKPSKEELEDLLFCWKVAKYVKSNAIVYAKNGQTIGIGAGQMSRVYSAKIAGIKAEDEGLQVAGCVMASDAFFP
FRDGIDAAAKVGITCVIHPGGSMRDQEVIDAANEHGMAMVLTGMRHFRH

Sequences:

>Translated_529_residues
MAIQQALLSVSDKKGIVEFAQGLVARGVKLLSTGGTAKLLAEAGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRG
TDDEVMNRHGIERIDIVVVNLYPFAATVANPNCTLADAVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFDAILAEMDQNQ
NELTLETRFNLAIKAFEHTAQYDSMIANYFGKLVPPYQGAEEEDLTQVCGQFPRTLNLNFVRKQTMRYGENSHQNAAFYV
ENEVKEASVSTAKQLQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVALGKDILEAYNRAYQTDPTSAFGGII
AFNRELDGETAQTIAERQFVEVIIAPSVSEAAKAALAKKKNVRVLECGEFTQAQKRLDFKRVNGGLLVQDADQGSVSIDD
LQVVSERKPSKEELEDLLFCWKVAKYVKSNAIVYAKNGQTIGIGAGQMSRVYSAKIAGIKAEDEGLQVAGCVMASDAFFP
FRDGIDAAAKVGITCVIHPGGSMRDQEVIDAANEHGMAMVLTGMRHFRH
>Mature_528_residues
AIQQALLSVSDKKGIVEFAQGLVARGVKLLSTGGTAKLLAEAGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGT
DDEVMNRHGIERIDIVVVNLYPFAATVANPNCTLADAVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFDAILAEMDQNQN
ELTLETRFNLAIKAFEHTAQYDSMIANYFGKLVPPYQGAEEEDLTQVCGQFPRTLNLNFVRKQTMRYGENSHQNAAFYVE
NEVKEASVSTAKQLQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVALGKDILEAYNRAYQTDPTSAFGGIIA
FNRELDGETAQTIAERQFVEVIIAPSVSEAAKAALAKKKNVRVLECGEFTQAQKRLDFKRVNGGLLVQDADQGSVSIDDL
QVVSERKPSKEELEDLLFCWKVAKYVKSNAIVYAKNGQTIGIGAGQMSRVYSAKIAGIKAEDEGLQVAGCVMASDAFFPF
RDGIDAAAKVGITCVIHPGGSMRDQEVIDAANEHGMAMVLTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=494, Percent_Identity=40.080971659919, Blast_Score=283, Evalue=2e-76,
Organism=Escherichia coli, GI1790439, Length=527, Percent_Identity=76.4705882352941, Blast_Score=831, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=522, Percent_Identity=34.8659003831418, Blast_Score=256, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI71985574, Length=353, Percent_Identity=28.6118980169972, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71985556, Length=116, Percent_Identity=37.9310344827586, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323768, Length=498, Percent_Identity=35.9437751004016, Blast_Score=265, Evalue=9e-72,
Organism=Saccharomyces cerevisiae, GI6323056, Length=496, Percent_Identity=35.4838709677419, Blast_Score=258, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24649832, Length=622, Percent_Identity=35.3697749196142, Blast_Score=303, Evalue=2e-82,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57410; Mature: 57279

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQQALLSVSDKKGIVEFAQGLVARGVKLLSTGGTAKLLAEAGLPVTEVSDYTGFPEMM
CCHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCEECCCCCCCHHHH
DGRVKTLHPKVHGGILGRRGTDDEVMNRHGIERIDIVVVNLYPFAATVANPNCTLADAVE
CCCCEEECCHHHCCCCCCCCCCHHHHHHCCCCEEEEEEEEEECCEEEECCCCCCHHHHHH
NIDIGGPTMVRSAAKNHKDVAIVVNNNDFDAILAEMDQNQNELTLETRFNLAIKAFEHTA
HCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEEEECEEEHHHHHHH
QYDSMIANYFGKLVPPYQGAEEEDLTQVCGQFPRTLNLNFVRKQTMRYGENSHQNAAFYV
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEECHHHHHHHHHHCCCCCCCCEEEEE
ENEVKEASVSTAKQLQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVALGKDI
ECHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
LEAYNRAYQTDPTSAFGGIIAFNRELDGETAQTIAERQFVEVIIAPSVSEAAKAALAKKK
HHHHHHHCCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHEEEEECCCHHHHHHHHHHHCC
NVRVLECGEFTQAQKRLDFKRVNGGLLVQDADQGSVSIDDLQVVSERKPSKEELEDLLFC
CCEEEECCCHHHHHHHCCHHCCCCCEEEEECCCCCEEHHHHHHHHCCCCCHHHHHHHHHH
WKVAKYVKSNAIVYAKNGQTIGIGAGQMSRVYSAKIAGIKAEDEGLQVAGCVMASDAFFP
HHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCEEECCCCCEEEEEEEECCCCCC
FRDGIDAAAKVGITCVIHPGGSMRDQEVIDAANEHGMAMVLTGMRHFRH
CCCCCCHHHHCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEHHHHHHCC
>Mature Secondary Structure 
AIQQALLSVSDKKGIVEFAQGLVARGVKLLSTGGTAKLLAEAGLPVTEVSDYTGFPEMM
CHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCEECCCCCCCHHHH
DGRVKTLHPKVHGGILGRRGTDDEVMNRHGIERIDIVVVNLYPFAATVANPNCTLADAVE
CCCCEEECCHHHCCCCCCCCCCHHHHHHCCCCEEEEEEEEEECCEEEECCCCCCHHHHHH
NIDIGGPTMVRSAAKNHKDVAIVVNNNDFDAILAEMDQNQNELTLETRFNLAIKAFEHTA
HCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEEEECEEEHHHHHHH
QYDSMIANYFGKLVPPYQGAEEEDLTQVCGQFPRTLNLNFVRKQTMRYGENSHQNAAFYV
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEECHHHHHHHHHHCCCCCCCCEEEEE
ENEVKEASVSTAKQLQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVALGKDI
ECHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
LEAYNRAYQTDPTSAFGGIIAFNRELDGETAQTIAERQFVEVIIAPSVSEAAKAALAKKK
HHHHHHHCCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHEEEEECCCHHHHHHHHHHHCC
NVRVLECGEFTQAQKRLDFKRVNGGLLVQDADQGSVSIDDLQVVSERKPSKEELEDLLFC
CCEEEECCCHHHHHHHCCHHCCCCCEEEEECCCCCEEHHHHHHHHCCCCCHHHHHHHHHH
WKVAKYVKSNAIVYAKNGQTIGIGAGQMSRVYSAKIAGIKAEDEGLQVAGCVMASDAFFP
HHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCEEECCCCCEEEEEEEECCCCCC
FRDGIDAAAKVGITCVIHPGGSMRDQEVIDAANEHGMAMVLTGMRHFRH
CCCCCCHHHHCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]