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The map label for this gene is gltS [H]

Identifier: 126208441

GI number: 126208441

Start: 1120735

End: 1121952

Strand: Direct

Name: gltS [H]

Synonym: APL_0967

Alternate gene names: 126208441

Gene position: 1120735-1121952 (Clockwise)

Preceding gene: 126208434

Following gene: 126208448

Centisome position: 49.27

GC content: 43.68

Gene sequence:

>1218_bases
ATGGAACCAATAATACTGAACGGTTACCACACATTAATTGCTGCGACACTCGTTCTTTTGATCGGACGCTTTTTTGTAAG
CAAAATTAAATTCTTACAGGATTTTAATATTCCCGAAGCGGTTGCCGGCGGTTTAGTGGCTGCAATCGTGATTCTCTGCT
TATACCATTGGATGGGACTTTCTTTCCAATTCGAGAAAAGCTTACAAAATGCCTTTATGTTAGCCTTCTTCTCATCCGTC
GGTTTATCCGCCGATTTTTCTCGCTTAAAACAGGGCGGTATGCCTTTAGTGATCTTCCTTGCCGTTGTCGGTATATTCAT
TATTATCCAAAACGGCGTCGGTGTCGGACTTGCCACAGCATTAGGTTTAGATCCTTTAGTCGGTTTAGTAACTGGTTCAA
TTACCTTAACCGGCGGTCACGGTACCGGTGCGGCATGGGCGAAAATCTTCTCCGAGCAATACGGCATTACCGGTATTATG
GAAATGGCAATGGCGTCCGCTACATTCGGTTTAGTTGCCGGCGGTTTAATCGGTGGTCCGGTTGCACGCCGTTTAGTCAA
TAAAATGAAAAAAGACCGTAAACAATATAGCAAAAAAATGGATACTTCTGCCGAACAGTATGACGACAGCACTTTTGAAT
CGGCTGACAGCATTCGTTTCATTACGGCGAGTTCCACAATCGAAACGATGGCGTTATTCGCAGCCTGTTTAGCTTTCTCA
TCGGTAATGGCTAACGCATTCCCGGATTTAGGTTTACCGCAATTCGTATGGGCATTAGGTTTCGGTGTAATTTTACGTAA
CGTATTAACCAAAGTAGTCAAATTCGATATGTTTGACCGCGCGATCGACGTATTCGGTAACGCATCATTAAGCTTATTCT
TAGCAATGGCGTTAATGAGCATTAAATTATGGGAATTAGCAGGCTTAGCCGGTCCGATGTTAATTATTTTAGTCGTTCAA
ACTATCGTTATGATCTTATACGGTTACTTCGTGACTTTCCGTGTAATGGGTAAAGACTATGATGCGGCGATTTTAACCGC
CGGTCACTGCGGTTTCGGCTTGGGTGCGACACCGACTGCGGTTGCCAATATGCAATCGGTCACCGAAACGTTCGGTCCGT
CACATAAAGCGTTCTTAATCGTACCGTTAGTCGGCGCATTCTTCGTAGATATTTTAAACTTAGGTGTTATCACTTGGTTT
GTTGAGTTCTTACGTTAA

Upstream 100 bases:

>100_bases
TATTTATACATACCTTTGACAAATGTTTTTTATTGGTCTAGTATCGAACAATCCTTATTTTTAAACATTCATTTAACATT
TTTATTTTAGAGGAGCACCT

Downstream 100 bases:

>100_bases
TCGTTTAAACAAATAAAGCAAAGGGGCAACCGTTACGGTTTCCCCTTCTTTGTTTTATTACTTAAATCATTAGTGATAGG
TTTTACTAAATTTCTGATAG

Product: sodium/glutamate symport carrier protein

Products: Na (I) [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate permease [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MEPIILNGYHTLIAATLVLLIGRFFVSKIKFLQDFNIPEAVAGGLVAAIVILCLYHWMGLSFQFEKSLQNAFMLAFFSSV
GLSADFSRLKQGGMPLVIFLAVVGIFIIIQNGVGVGLATALGLDPLVGLVTGSITLTGGHGTGAAWAKIFSEQYGITGIM
EMAMASATFGLVAGGLIGGPVARRLVNKMKKDRKQYSKKMDTSAEQYDDSTFESADSIRFITASSTIETMALFAACLAFS
SVMANAFPDLGLPQFVWALGFGVILRNVLTKVVKFDMFDRAIDVFGNASLSLFLAMALMSIKLWELAGLAGPMLIILVVQ
TIVMILYGYFVTFRVMGKDYDAAILTAGHCGFGLGATPTAVANMQSVTETFGPSHKAFLIVPLVGAFFVDILNLGVITWF
VEFLR

Sequences:

>Translated_405_residues
MEPIILNGYHTLIAATLVLLIGRFFVSKIKFLQDFNIPEAVAGGLVAAIVILCLYHWMGLSFQFEKSLQNAFMLAFFSSV
GLSADFSRLKQGGMPLVIFLAVVGIFIIIQNGVGVGLATALGLDPLVGLVTGSITLTGGHGTGAAWAKIFSEQYGITGIM
EMAMASATFGLVAGGLIGGPVARRLVNKMKKDRKQYSKKMDTSAEQYDDSTFESADSIRFITASSTIETMALFAACLAFS
SVMANAFPDLGLPQFVWALGFGVILRNVLTKVVKFDMFDRAIDVFGNASLSLFLAMALMSIKLWELAGLAGPMLIILVVQ
TIVMILYGYFVTFRVMGKDYDAAILTAGHCGFGLGATPTAVANMQSVTETFGPSHKAFLIVPLVGAFFVDILNLGVITWF
VEFLR
>Mature_405_residues
MEPIILNGYHTLIAATLVLLIGRFFVSKIKFLQDFNIPEAVAGGLVAAIVILCLYHWMGLSFQFEKSLQNAFMLAFFSSV
GLSADFSRLKQGGMPLVIFLAVVGIFIIIQNGVGVGLATALGLDPLVGLVTGSITLTGGHGTGAAWAKIFSEQYGITGIM
EMAMASATFGLVAGGLIGGPVARRLVNKMKKDRKQYSKKMDTSAEQYDDSTFESADSIRFITASSTIETMALFAACLAFS
SVMANAFPDLGLPQFVWALGFGVILRNVLTKVVKFDMFDRAIDVFGNASLSLFLAMALMSIKLWELAGLAGPMLIILVVQ
TIVMILYGYFVTFRVMGKDYDAAILTAGHCGFGLGATPTAVANMQSVTETFGPSHKAFLIVPLVGAFFVDILNLGVITWF
VEFLR

Specific function: Catalyzes the sodium-dependent uptake of extracellular glutamate [H]

COG id: COG0786

COG function: function code E; Na+/glutamate symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gltS symporter family [H]

Homologues:

Organism=Escherichia coli, GI1790085, Length=382, Percent_Identity=55.2356020942408, Blast_Score=355, Evalue=3e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004445 [H]

Pfam domain/function: PF03616 Glt_symporter [H]

EC number: NA

Molecular weight: Translated: 43511; Mature: 43511

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPIILNGYHTLIAATLVLLIGRFFVSKIKFLQDFNIPEAVAGGLVAAIVILCLYHWMGL
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
SFQFEKSLQNAFMLAFFSSVGLSADFSRLKQGGMPLVIFLAVVGIFIIIQNGVGVGLATA
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
LGLDPLVGLVTGSITLTGGHGTGAAWAKIFSEQYGITGIMEMAMASATFGLVAGGLIGGP
HHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHH
VARRLVNKMKKDRKQYSKKMDTSAEQYDDSTFESADSIRFITASSTIETMALFAACLAFS
HHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHCCCCCEEEEECCHHHHHHHHHHHHHHHH
SVMANAFPDLGLPQFVWALGFGVILRNVLTKVVKFDMFDRAIDVFGNASLSLFLAMALMS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IKLWELAGLAGPMLIILVVQTIVMILYGYFVTFRVMGKDYDAAILTAGHCGFGLGATPTA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHH
VANMQSVTETFGPSHKAFLIVPLVGAFFVDILNLGVITWFVEFLR
HHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MEPIILNGYHTLIAATLVLLIGRFFVSKIKFLQDFNIPEAVAGGLVAAIVILCLYHWMGL
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
SFQFEKSLQNAFMLAFFSSVGLSADFSRLKQGGMPLVIFLAVVGIFIIIQNGVGVGLATA
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
LGLDPLVGLVTGSITLTGGHGTGAAWAKIFSEQYGITGIMEMAMASATFGLVAGGLIGGP
HHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHH
VARRLVNKMKKDRKQYSKKMDTSAEQYDDSTFESADSIRFITASSTIETMALFAACLAFS
HHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHCCCCCEEEEECCHHHHHHHHHHHHHHHH
SVMANAFPDLGLPQFVWALGFGVILRNVLTKVVKFDMFDRAIDVFGNASLSLFLAMALMS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IKLWELAGLAGPMLIILVVQTIVMILYGYFVTFRVMGKDYDAAILTAGHCGFGLGATPTA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHH
VANMQSVTETFGPSHKAFLIVPLVGAFFVDILNLGVITWFVEFLR
HHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-glutamate [Periplasm] = Na (I) [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]