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The map label for this gene is purR

Identifier: 126208295

GI number: 126208295

Start: 946139

End: 947149

Strand: Direct

Name: purR

Synonym: APL_0819

Alternate gene names: 126208295

Gene position: 946139-947149 (Clockwise)

Preceding gene: 126208293

Following gene: 126208296

Centisome position: 41.6

GC content: 39.76

Gene sequence:

>1011_bases
ATGGCTACAATTAAAGACGTTGCTAAATTAGCCGGTGTTTCGACCACCACCGTTTCACATGTAATCAATAAAACCCGTTT
TGTAGCAGAAGATACCAGTAAGGCTGTATGGGATGCAATCCAACAATTAAATTATTCCCCGAGTGCCGTTGCTCGCAGCT
TAAAAGTCAATACCACTAAATCCATCGGAATGATCATCACGACCAGCGAAGCGCCTTATTTTGCCGAAATCGTATTAGCC
GTAGAAGAACATTGCTATCAACAAGGCTATTCTCTTTTCTTATGCAATACCCAAAACGAGCCGGAAAAAATCCAAAACCA
TTTGGATATGTTAATTAAAAAACGAGTGGACGGCGTATTGGTAATGTGTTCCGAATATACTCGAGATTCTTTAGAACTAT
TCAACGGTACAAATATCCCGATGGTGGTAATGGATTGGGGTAAAGCCGATGATCATAGCGATCGTATTTTGGATAATAGC
TTTGAAGGAGGATACTTAGCTACCCAACATTTAATTGAAAACGGTCATAAAGATATCGGCGTTATCGCCGGTCACCTAAG
CAAAACCCTTTCCAAAGAACGTTACGAAGGTTTCTTAAAAGCGATGCACGAAGCAAATTTACCTGTGCGCCAAGAATGGA
TTTATGAAGGCGACTTCGAACCGGAAAGCGGTTTTGAGCAAATGAATAATTTATTACGTTTGGAAAAATTACCGACGGCG
ATTTTTTGTTTCAGTGATACGATTGCATTAGGCGCCATTTCGGCATTAAGTGAAAAAGGGTTGTCTGTACCGAGCTATAT
GTCAATTATCGGATACGATAATATTCATTCGTCACGTTTTTATTCTCCGCCGCTTACGACGATCCACCAATCGAAATCAC
GTTTAGGCGTAAAAGCGCTCAATATTTTACTTGAACGAATTAAAATAGATAAAACCCAATATCAACCTCAAACGATTGAG
TTTCACCCCGAGTTAGTCTTACGTCGTTCTGTGAGAAACCTAAATAAATAA

Upstream 100 bases:

>100_bases
TCCCTATTCTTTACATATTTATTTGCAGAATTTCCTCTCTTAGCCGATTAATTTTCGTTATAATAAGAGATTATTTTGAT
ATTAAAAAGAGAACAAAATT

Downstream 100 bases:

>100_bases
TCCGTTTATAGAAGGAGTAAATATGGAATTCTTAATCAGCTTTTTCAGTAGCTATGGCTATTGGGCTGTATTTTTGGTTT
TGTTGGCTTGTGGCTTCGGC

Product: DNA-binding transcriptional repressor PurR

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MATIKDVAKLAGVSTTTVSHVINKTRFVAEDTSKAVWDAIQQLNYSPSAVARSLKVNTTKSIGMIITTSEAPYFAEIVLA
VEEHCYQQGYSLFLCNTQNEPEKIQNHLDMLIKKRVDGVLVMCSEYTRDSLELFNGTNIPMVVMDWGKADDHSDRILDNS
FEGGYLATQHLIENGHKDIGVIAGHLSKTLSKERYEGFLKAMHEANLPVRQEWIYEGDFEPESGFEQMNNLLRLEKLPTA
IFCFSDTIALGAISALSEKGLSVPSYMSIIGYDNIHSSRFYSPPLTTIHQSKSRLGVKALNILLERIKIDKTQYQPQTIE
FHPELVLRRSVRNLNK

Sequences:

>Translated_336_residues
MATIKDVAKLAGVSTTTVSHVINKTRFVAEDTSKAVWDAIQQLNYSPSAVARSLKVNTTKSIGMIITTSEAPYFAEIVLA
VEEHCYQQGYSLFLCNTQNEPEKIQNHLDMLIKKRVDGVLVMCSEYTRDSLELFNGTNIPMVVMDWGKADDHSDRILDNS
FEGGYLATQHLIENGHKDIGVIAGHLSKTLSKERYEGFLKAMHEANLPVRQEWIYEGDFEPESGFEQMNNLLRLEKLPTA
IFCFSDTIALGAISALSEKGLSVPSYMSIIGYDNIHSSRFYSPPLTTIHQSKSRLGVKALNILLERIKIDKTQYQPQTIE
FHPELVLRRSVRNLNK
>Mature_335_residues
ATIKDVAKLAGVSTTTVSHVINKTRFVAEDTSKAVWDAIQQLNYSPSAVARSLKVNTTKSIGMIITTSEAPYFAEIVLAV
EEHCYQQGYSLFLCNTQNEPEKIQNHLDMLIKKRVDGVLVMCSEYTRDSLELFNGTNIPMVVMDWGKADDHSDRILDNSF
EGGYLATQHLIENGHKDIGVIAGHLSKTLSKERYEGFLKAMHEANLPVRQEWIYEGDFEPESGFEQMNNLLRLEKLPTAI
FCFSDTIALGAISALSEKGLSVPSYMSIIGYDNIHSSRFYSPPLTTIHQSKSRLGVKALNILLERIKIDKTQYQPQTIEF
HPELVLRRSVRNLNK

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=57.6576576576577, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1790194, Length=331, Percent_Identity=46.8277945619335, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1790369, Length=337, Percent_Identity=34.7181008902077, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789202, Length=290, Percent_Identity=33.448275862069, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1788474, Length=334, Percent_Identity=31.437125748503, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1786540, Length=311, Percent_Identity=31.8327974276527, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787580, Length=336, Percent_Identity=27.9761904761905, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1789068, Length=291, Percent_Identity=26.4604810996564, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1787906, Length=320, Percent_Identity=26.25, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1789456, Length=321, Percent_Identity=28.0373831775701, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI48994940, Length=306, Percent_Identity=25.4901960784314, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790715, Length=318, Percent_Identity=25.1572327044025, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786268, Length=305, Percent_Identity=22.9508196721311, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790689, Length=325, Percent_Identity=26.1538461538462, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790192, Length=291, Percent_Identity=26.1168384879725, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURR_ACTP2 (A3N0H9)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053520.1
- ProteinModelPortal:   A3N0H9
- SMR:   A3N0H9
- STRING:   A3N0H9
- GeneID:   4849135
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0819
- eggNOG:   COG1609
- HOGENOM:   HBG753640
- OMA:   IKNHLEM
- PhylomeDB:   A3N0H9
- ProtClustDB:   PRK10703
- BioCyc:   APLE416269:APL_0819-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01277
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- PRINTS:   PR00036
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 37832; Mature: 37700

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: BINDING 73-73 BINDING 188-188 BINDING 219-219 BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAKLAGVSTTTVSHVINKTRFVAEDTSKAVWDAIQQLNYSPSAVARSLKVNTTK
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
SIGMIITTSEAPYFAEIVLAVEEHCYQQGYSLFLCNTQNEPEKIQNHLDMLIKKRVDGVL
CEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEE
VMCSEYTRDSLELFNGTNIPMVVMDWGKADDHSDRILDNSFEGGYLATQHLIENGHKDIG
EEECHHCHHHHHHHCCCCCCEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHCCCCCHH
VIAGHLSKTLSKERYEGFLKAMHEANLPVRQEWIYEGDFEPESGFEQMNNLLRLEKLPTA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCE
IFCFSDTIALGAISALSEKGLSVPSYMSIIGYDNIHSSRFYSPPLTTIHQSKSRLGVKAL
EEEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
NILLERIKIDKTQYQPQTIEFHPELVLRRSVRNLNK
HHHHHHHHHCHHCCCCCEEEECHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATIKDVAKLAGVSTTTVSHVINKTRFVAEDTSKAVWDAIQQLNYSPSAVARSLKVNTTK
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
SIGMIITTSEAPYFAEIVLAVEEHCYQQGYSLFLCNTQNEPEKIQNHLDMLIKKRVDGVL
CEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEE
VMCSEYTRDSLELFNGTNIPMVVMDWGKADDHSDRILDNSFEGGYLATQHLIENGHKDIG
EEECHHCHHHHHHHCCCCCCEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHCCCCCHH
VIAGHLSKTLSKERYEGFLKAMHEANLPVRQEWIYEGDFEPESGFEQMNNLLRLEKLPTA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCE
IFCFSDTIALGAISALSEKGLSVPSYMSIIGYDNIHSSRFYSPPLTTIHQSKSRLGVKAL
EEEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
NILLERIKIDKTQYQPQTIEFHPELVLRRSVRNLNK
HHHHHHHHHCHHCCCCCEEEECHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA