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The map label for this gene is mltA [H]

Identifier: 126208292

GI number: 126208292

Start: 942185

End: 943282

Strand: Reverse

Name: mltA [H]

Synonym: APL_0816

Alternate gene names: 126208292

Gene position: 943282-942185 (Counterclockwise)

Preceding gene: 126208294

Following gene: 126208289

Centisome position: 41.47

GC content: 44.63

Gene sequence:

>1098_bases
ATGAATTGGAAGGCGTATAAACAGTGGGCGGCGGTGACTGCCGTCGCACTTTTAGTTGCAAGTTGTTCTTCGGATAGAGT
AAGCAAAGGACAGAGTGATTATCAGGCGGAACACGCGAAGTTTGGTGCGGTATATAAAGGTCGTCAGTATATTCCGCATA
ATTTTGTCAGCACTCCGAGAGTGGCGGCAAACGGATCGATTGTTAATTTCCAAGATTTTTTAAAACAGTTAAATAATGTA
CGCGCGTATGCCGGCGGTATTACCGGCCGTTATGCAGGCACTTACGGTAAAGTTTCCTCATGGATCGCTTCAGGCGGTAA
GGTATCGGATTTAGCCCGTTACAATATTCACGCGCTACAAATGCGTGGTGAAGACGGTTTCCAAAACGTGTTAATGACCG
GTTACTATTCTCCGGTTATTCATGCACGCCGTACGCCTCAAGGTCAATATCAACACCCGATTTATGCAATGCCAAGTCAG
AAACGCTTTACCCGTTCGCAAATTTATGACGGTGCGTTAGAAGGTAAAGGTTTAGAACTCGCTTATAGTGATTCAATGTT
GGATAACTTCTTACTTGGCGTGCAAGGGAGCGGTTATGTTGATTTCGGTGACGGTCGTTTAAATTATTTCGCTTATGCAG
GTCAAAACGGCTTTAAATATGCGAGTGTCGGTCGTTTATTAGTTGAAGACGGTGAAATTCCAAAAGAGAAAATGTCTATT
CAAGCGATTCGTGAATGGGGAGAACGTAATCCGCATCGTGTACAAGGCTTATTAGAACGTAACCCGTCTTACGTTTTCTT
TAAAAATGATCCGACCGGTCAAGTAAAAGGTTCGGCAGGCGTACCGTTAGTCGCATTGGCGTCAGTCGCTTCAGATAAAA
GCCTTGTACCGTCAGGCAGCGTGTTATTGGTTGAAACACCGTTAATCGATCGTGCTGGTAACTGGACGGGCAAACACGAA
TTACGTTTAATGGTAGCGTTAGATGTCGGCGGTGCGGTAAAAGGTCATCACTTTGATTTATACCAAGGTATCGGTGATGA
GGCTGGTCATAAAGCCGGTTTAATGAAACACTACGGACGTGTTTGGGTATTAAACTAA

Upstream 100 bases:

>100_bases
TAAGTGTAGTCTGTGTAAAGGTTCAAAATTTAGTATCGTTTTAGCATGAATTTTGATAGGCTAATCACTTTTCTCATCAA
TTAAATTTCTAAGGTTTTTT

Downstream 100 bases:

>100_bases
TTACTCATCGTACACAAGCGGTTGATTTTTGCAAAAGATTTGCAAATTTTGACCGCTTGTTAGCTTCTAGATACAGCCTC
CGCAACTGCCACAACGCCAG

Product: murein transglycosylase A

Products: Muramic Acid Residue [C]

Alternate protein names: Mlt38; Murein hydrolase A [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MNWKAYKQWAAVTAVALLVASCSSDRVSKGQSDYQAEHAKFGAVYKGRQYIPHNFVSTPRVAANGSIVNFQDFLKQLNNV
RAYAGGITGRYAGTYGKVSSWIASGGKVSDLARYNIHALQMRGEDGFQNVLMTGYYSPVIHARRTPQGQYQHPIYAMPSQ
KRFTRSQIYDGALEGKGLELAYSDSMLDNFLLGVQGSGYVDFGDGRLNYFAYAGQNGFKYASVGRLLVEDGEIPKEKMSI
QAIREWGERNPHRVQGLLERNPSYVFFKNDPTGQVKGSAGVPLVALASVASDKSLVPSGSVLLVETPLIDRAGNWTGKHE
LRLMVALDVGGAVKGHHFDLYQGIGDEAGHKAGLMKHYGRVWVLN

Sequences:

>Translated_365_residues
MNWKAYKQWAAVTAVALLVASCSSDRVSKGQSDYQAEHAKFGAVYKGRQYIPHNFVSTPRVAANGSIVNFQDFLKQLNNV
RAYAGGITGRYAGTYGKVSSWIASGGKVSDLARYNIHALQMRGEDGFQNVLMTGYYSPVIHARRTPQGQYQHPIYAMPSQ
KRFTRSQIYDGALEGKGLELAYSDSMLDNFLLGVQGSGYVDFGDGRLNYFAYAGQNGFKYASVGRLLVEDGEIPKEKMSI
QAIREWGERNPHRVQGLLERNPSYVFFKNDPTGQVKGSAGVPLVALASVASDKSLVPSGSVLLVETPLIDRAGNWTGKHE
LRLMVALDVGGAVKGHHFDLYQGIGDEAGHKAGLMKHYGRVWVLN
>Mature_365_residues
MNWKAYKQWAAVTAVALLVASCSSDRVSKGQSDYQAEHAKFGAVYKGRQYIPHNFVSTPRVAANGSIVNFQDFLKQLNNV
RAYAGGITGRYAGTYGKVSSWIASGGKVSDLARYNIHALQMRGEDGFQNVLMTGYYSPVIHARRTPQGQYQHPIYAMPSQ
KRFTRSQIYDGALEGKGLELAYSDSMLDNFLLGVQGSGYVDFGDGRLNYFAYAGQNGFKYASVGRLLVEDGEIPKEKMSI
QAIREWGERNPHRVQGLLERNPSYVFFKNDPTGQVKGSAGVPLVALASVASDKSLVPSGSVLLVETPLIDRAGNWTGKHE
LRLMVALDVGGAVKGHHFDLYQGIGDEAGHKAGLMKHYGRVWVLN

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Degrades murein glycan strands and insoluble, high-molecular weight murein sacculi [H]

COG id: COG2821

COG function: function code M; Membrane-bound lytic murein transglycosylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789179, Length=356, Percent_Identity=50.561797752809, Blast_Score=341, Evalue=4e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010611
- InterPro:   IPR014733
- InterPro:   IPR005300 [H]

Pfam domain/function: PF06725 3D; PF03562 MltA [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 40066; Mature: 40066

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWKAYKQWAAVTAVALLVASCSSDRVSKGQSDYQAEHAKFGAVYKGRQYIPHNFVSTPR
CCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCEECCCCCCCCCCCCCCC
VAANGSIVNFQDFLKQLNNVRAYAGGITGRYAGTYGKVSSWIASGGKVSDLARYNIHALQ
EECCCCEECHHHHHHHHHCCEEHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCEEEEE
MRGEDGFQNVLMTGYYSPVIHARRTPQGQYQHPIYAMPSQKRFTRSQIYDGALEGKGLEL
ECCCCCHHHHHHHCCCCHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCCCEEE
AYSDSMLDNFLLGVQGSGYVDFGDGRLNYFAYAGQNGFKYASVGRLLVEDGEIPKEKMSI
EECHHHHHHHEEECCCCCEEEECCCCEEEEEEECCCCCCHHHHCEEEEECCCCCHHHHHH
QAIREWGERNPHRVQGLLERNPSYVFFKNDPTGQVKGSAGVPLVALASVASDKSLVPSGS
HHHHHHHCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCC
VLLVETPLIDRAGNWTGKHELRLMVALDVGGAVKGHHFDLYQGIGDEAGHKAGLMKHYGR
EEEEECCCCCCCCCCCCCEEEEEEEEEECCCCCCCCEEHHHHCCCCCCCHHHHHHHHCCE
VWVLN
EEEEC
>Mature Secondary Structure
MNWKAYKQWAAVTAVALLVASCSSDRVSKGQSDYQAEHAKFGAVYKGRQYIPHNFVSTPR
CCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCEECCCCCCCCCCCCCCC
VAANGSIVNFQDFLKQLNNVRAYAGGITGRYAGTYGKVSSWIASGGKVSDLARYNIHALQ
EECCCCEECHHHHHHHHHCCEEHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCEEEEE
MRGEDGFQNVLMTGYYSPVIHARRTPQGQYQHPIYAMPSQKRFTRSQIYDGALEGKGLEL
ECCCCCHHHHHHHCCCCHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCCCEEE
AYSDSMLDNFLLGVQGSGYVDFGDGRLNYFAYAGQNGFKYASVGRLLVEDGEIPKEKMSI
EECHHHHHHHEEECCCCCEEEECCCCEEEEEEECCCCCCHHHHCEEEEECCCCCHHHHHH
QAIREWGERNPHRVQGLLERNPSYVFFKNDPTGQVKGSAGVPLVALASVASDKSLVPSGS
HHHHHHHCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCC
VLLVETPLIDRAGNWTGKHELRLMVALDVGGAVKGHHFDLYQGIGDEAGHKAGLMKHYGR
EEEEECCCCCCCCCCCCCEEEEEEEEEECCCCCCCCEEHHHHCCCCCCCHHHHHHHHCCE
VWVLN
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]