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The map label for this gene is ilvI [H]

Identifier: 126208207

GI number: 126208207

Start: 835093

End: 836817

Strand: Direct

Name: ilvI [H]

Synonym: APL_0727

Alternate gene names: 126208207

Gene position: 835093-836817 (Clockwise)

Preceding gene: 126208206

Following gene: 126208208

Centisome position: 36.72

GC content: 43.65

Gene sequence:

>1725_bases
ATGAAAAAACTTTCCGGAGCAGAAATGGTTGTTCAATCCTTGAAAGACGAAGGCGTAGAGTACGTTTTCGGCTATCCGGG
TGGTTCGGTATTAGATATTTATGATGCGATTCATACGCTGGATAATATTAACCACGTTCTTGTGCGCCATGAGCAAGCCG
CGGTCCATATGGCGGACGGTTACGCTCGCTCAACCGGTAAAGTCGGTTGTGTATTAGTAACATCCGGTCCCGGTGCAACC
AATGCGATCACAGGTATTGCCACCGCTTATGCCGATTCTGTCCCGCTTGTGATCTTAACCGGTCAAGTGCCTTCCGCTTT
AATCGGTACGGATGCTTTCCAAGAATGCGATATGCTCGGTATTTCACGCCCTGTGGTTAAACACAGTTTTATGATCAAAA
ATCCGGAAGATATTCCTTCAATAATCAAAAAAGCCTTTTATATTGCTTCAACCGGTCGCCCCAGTCCGGTCTTAATTGAT
ATTCCGAAAGATATGGTAAATCCGGCGAATAAATTTACCTATGAATATCCGAAAGAAGTCTCACTACGTTCATATAACCC
GACCGTACAAGGCCATAAAGTGCAAATTAAAAAGGCATTAAAAGCCCTATTAGTGGCAAAAAAACCGGTTTTATACATTG
GTGGAGGGATTATCTCTGCGGAATGTGCAACGCAACTTACCGACTTTGCAACGAAGTTAAACTTACCGGTAACCAGTTCG
TTAATGGGCTTAGGCGCTTTCCCTGCATCAAACAAACAATTTTTAGGTATGCTCGGTATGCACGGTACTTACGAAGCGAA
TAACGCAATGCACGAAAGCGATTTGATTCTCGGCATTGGCGTACGTTTTGATGACCGTACCACGAATAATTTAGCAAAAT
ATTGCCCGAATGCAAAAGTCATTCACGTGGATATTGATCCGGCTTCTATTTCAAAAACCGTTCCGGCTTATATTCCGATT
GTGGGTAGCGCAAAAAATGTCTTGGAAGAATTTCTCAGCCTTTTAGAAGAAGAAAATCTTGCAAAAAATCAAGCGGATTT
GACCGCTTGGTGGCAACAGATTAATGAATGGAAAGCTCGTCAGTGCTTAAATTTTGAAGTAAGTAAAGATGTAATCAAAC
CGCAGCAAGTCATTCGATTAATTCATAAAATTACCAATGGTGATGCCTATGTTGCTTCGGATGTCGGTCAGCACCAAATG
TTTGCCGCACTCCATTATCCGTTTGAAAAACCTCGTCGCTGGATCAACTCGGGCGGAGCCGGTACCATGGGATTCGGTTT
ACCGGCAGCAATCGGCGTAAAATTCGCCCATCCGGAAGCAACTGTAGTATGTGTAACCGGTGACGGCTCGATTCAGATGA
ATATTCAAGAGCTTTCTACGGCGAAACAATATGATACGCCGGTTGTGATTGTGAGCTTAAACAATCGCTTCTTAGGTATG
GTGAAGCAGTGGCAAGATCTGATCTACTCCGGTCGCCATTCACAGGTATATATGAATTCACTGCCTGATTTTGCCAAATT
AGCCGAAGCTTACGGTCATGTCGGCATTACGATTGATCACCCGAACGAATTGGAAGAAAAACTCACCCAAGCCTTTGCTA
TCAAAGACAAATTAGTGTTTGTCGATGTAAAAGTAGATGAAACCGAACACGTTTACCCGATGCAAATTCGCGGCGGTGCG
ATGAATGAGATGATTTTAAGCAAAACGGAGAGAACAGATGCGTAG

Upstream 100 bases:

>100_bases
AAAGCTTTCCGCCCCCTAGACAAACATTTTATTTTTTTGCAGAATCATTTTCATTATAAAGCAAACACAATATTTCACTT
AAAAATCGTTGGAGGATTCA

Downstream 100 bases:

>100_bases
AACATTATCAGTATTACTCGAAAACGAATCGGGTGCATTATCCCGTGTGGTAGGCCTTTTTTCTCAACGTGGGTTTAATA
TCGAAAGTTTAACCGTTGCG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MKKLSGAEMVVQSLKDEGVEYVFGYPGGSVLDIYDAIHTLDNINHVLVRHEQAAVHMADGYARSTGKVGCVLVTSGPGAT
NAITGIATAYADSVPLVILTGQVPSALIGTDAFQECDMLGISRPVVKHSFMIKNPEDIPSIIKKAFYIASTGRPSPVLID
IPKDMVNPANKFTYEYPKEVSLRSYNPTVQGHKVQIKKALKALLVAKKPVLYIGGGIISAECATQLTDFATKLNLPVTSS
LMGLGAFPASNKQFLGMLGMHGTYEANNAMHESDLILGIGVRFDDRTTNNLAKYCPNAKVIHVDIDPASISKTVPAYIPI
VGSAKNVLEEFLSLLEEENLAKNQADLTAWWQQINEWKARQCLNFEVSKDVIKPQQVIRLIHKITNGDAYVASDVGQHQM
FAALHYPFEKPRRWINSGGAGTMGFGLPAAIGVKFAHPEATVVCVTGDGSIQMNIQELSTAKQYDTPVVIVSLNNRFLGM
VKQWQDLIYSGRHSQVYMNSLPDFAKLAEAYGHVGITIDHPNELEEKLTQAFAIKDKLVFVDVKVDETEHVYPMQIRGGA
MNEMILSKTERTDA

Sequences:

>Translated_574_residues
MKKLSGAEMVVQSLKDEGVEYVFGYPGGSVLDIYDAIHTLDNINHVLVRHEQAAVHMADGYARSTGKVGCVLVTSGPGAT
NAITGIATAYADSVPLVILTGQVPSALIGTDAFQECDMLGISRPVVKHSFMIKNPEDIPSIIKKAFYIASTGRPSPVLID
IPKDMVNPANKFTYEYPKEVSLRSYNPTVQGHKVQIKKALKALLVAKKPVLYIGGGIISAECATQLTDFATKLNLPVTSS
LMGLGAFPASNKQFLGMLGMHGTYEANNAMHESDLILGIGVRFDDRTTNNLAKYCPNAKVIHVDIDPASISKTVPAYIPI
VGSAKNVLEEFLSLLEEENLAKNQADLTAWWQQINEWKARQCLNFEVSKDVIKPQQVIRLIHKITNGDAYVASDVGQHQM
FAALHYPFEKPRRWINSGGAGTMGFGLPAAIGVKFAHPEATVVCVTGDGSIQMNIQELSTAKQYDTPVVIVSLNNRFLGM
VKQWQDLIYSGRHSQVYMNSLPDFAKLAEAYGHVGITIDHPNELEEKLTQAFAIKDKLVFVDVKVDETEHVYPMQIRGGA
MNEMILSKTERTDA
>Mature_574_residues
MKKLSGAEMVVQSLKDEGVEYVFGYPGGSVLDIYDAIHTLDNINHVLVRHEQAAVHMADGYARSTGKVGCVLVTSGPGAT
NAITGIATAYADSVPLVILTGQVPSALIGTDAFQECDMLGISRPVVKHSFMIKNPEDIPSIIKKAFYIASTGRPSPVLID
IPKDMVNPANKFTYEYPKEVSLRSYNPTVQGHKVQIKKALKALLVAKKPVLYIGGGIISAECATQLTDFATKLNLPVTSS
LMGLGAFPASNKQFLGMLGMHGTYEANNAMHESDLILGIGVRFDDRTTNNLAKYCPNAKVIHVDIDPASISKTVPAYIPI
VGSAKNVLEEFLSLLEEENLAKNQADLTAWWQQINEWKARQCLNFEVSKDVIKPQQVIRLIHKITNGDAYVASDVGQHQM
FAALHYPFEKPRRWINSGGAGTMGFGLPAAIGVKFAHPEATVVCVTGDGSIQMNIQELSTAKQYDTPVVIVSLNNRFLGM
VKQWQDLIYSGRHSQVYMNSLPDFAKLAEAYGHVGITIDHPNELEEKLTQAFAIKDKLVFVDVKVDETEHVYPMQIRGGA
MNEMILSKTERTDA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=570, Percent_Identity=26.6666666666667, Blast_Score=173, Evalue=4e-43,
Organism=Homo sapiens, GI21361361, Length=555, Percent_Identity=23.4234234234234, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI87081685, Length=574, Percent_Identity=70.5574912891986, Blast_Score=876, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=566, Percent_Identity=39.5759717314488, Blast_Score=427, Evalue=1e-120,
Organism=Escherichia coli, GI1786717, Length=548, Percent_Identity=33.029197080292, Blast_Score=312, Evalue=4e-86,
Organism=Escherichia coli, GI1787096, Length=562, Percent_Identity=28.1138790035587, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1788716, Length=588, Percent_Identity=22.9591836734694, Blast_Score=148, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=551, Percent_Identity=28.1306715063521, Blast_Score=172, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17531301, Length=551, Percent_Identity=28.1306715063521, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17542570, Length=605, Percent_Identity=23.4710743801653, Blast_Score=132, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=593, Percent_Identity=41.8212478920742, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320816, Length=485, Percent_Identity=23.9175257731959, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320123, Length=507, Percent_Identity=22.0907297830375, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=495, Percent_Identity=23.6363636363636, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6321524, Length=506, Percent_Identity=22.9249011857708, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=495, Percent_Identity=23.030303030303, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19922626, Length=575, Percent_Identity=24.8695652173913, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62776; Mature: 62776

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLSGAEMVVQSLKDEGVEYVFGYPGGSVLDIYDAIHTLDNINHVLVRHEQAAVHMADG
CCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEECCC
YARSTGKVGCVLVTSGPGATNAITGIATAYADSVPLVILTGQVPSALIGTDAFQECDMLG
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHCCHHHHHHHHHC
ISRPVVKHSFMIKNPEDIPSIIKKAFYIASTGRPSPVLIDIPKDMVNPANKFTYEYPKEV
CCCCHHHHEEEECCCHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCCCHHCCCCCCCCC
SLRSYNPTVQGHKVQIKKALKALLVAKKPVLYIGGGIISAECATQLTDFATKLNLPVTSS
CEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHCCCCHHHH
LMGLGAFPASNKQFLGMLGMHGTYEANNAMHESDLILGIGVRFDDRTTNNLAKYCPNAKV
HHHCCCCCCCCCHHHEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCEE
IHVDIDPASISKTVPAYIPIVGSAKNVLEEFLSLLEEENLAKNQADLTAWWQQINEWKAR
EEEECCHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QCLNFEVSKDVIKPQQVIRLIHKITNGDAYVASDVGQHQMFAALHYPFEKPRRWINSGGA
HHCCCCHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCHHHHHHHHCCCCC
GTMGFGLPAAIGVKFAHPEATVVCVTGDGSIQMNIQELSTAKQYDTPVVIVSLNNRFLGM
CCCCCCCHHHHCEEEECCCEEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCHHHHH
VKQWQDLIYSGRHSQVYMNSLPDFAKLAEAYGHVGITIDHPNELEEKLTQAFAIKDKLVF
HHHHHHHHHCCCCCEEEHHCCCCHHHHHHHHCCEEEEECCCHHHHHHHHHHHHEECEEEE
VDVKVDETEHVYPMQIRGGAMNEMILSKTERTDA
EEEEECCCCCEEEEEECCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKLSGAEMVVQSLKDEGVEYVFGYPGGSVLDIYDAIHTLDNINHVLVRHEQAAVHMADG
CCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEECCC
YARSTGKVGCVLVTSGPGATNAITGIATAYADSVPLVILTGQVPSALIGTDAFQECDMLG
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHCCHHHHHHHHHC
ISRPVVKHSFMIKNPEDIPSIIKKAFYIASTGRPSPVLIDIPKDMVNPANKFTYEYPKEV
CCCCHHHHEEEECCCHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCCCHHCCCCCCCCC
SLRSYNPTVQGHKVQIKKALKALLVAKKPVLYIGGGIISAECATQLTDFATKLNLPVTSS
CEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHCCCCHHHH
LMGLGAFPASNKQFLGMLGMHGTYEANNAMHESDLILGIGVRFDDRTTNNLAKYCPNAKV
HHHCCCCCCCCCHHHEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCEE
IHVDIDPASISKTVPAYIPIVGSAKNVLEEFLSLLEEENLAKNQADLTAWWQQINEWKAR
EEEECCHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QCLNFEVSKDVIKPQQVIRLIHKITNGDAYVASDVGQHQMFAALHYPFEKPRRWINSGGA
HHCCCCHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCHHHHHHHHCCCCC
GTMGFGLPAAIGVKFAHPEATVVCVTGDGSIQMNIQELSTAKQYDTPVVIVSLNNRFLGM
CCCCCCCHHHHCEEEECCCEEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCHHHHH
VKQWQDLIYSGRHSQVYMNSLPDFAKLAEAYGHVGITIDHPNELEEKLTQAFAIKDKLVF
HHHHHHHHHCCCCCEEEHHCCCCHHHHHHHHCCEEEEECCCHHHHHHHHHHHHEECEEEE
VDVKVDETEHVYPMQIRGGAMNEMILSKTERTDA
EEEEECCCCCEEEEEECCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]