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The map label for this gene is aroA

Identifier: 126208179

GI number: 126208179

Start: 801193

End: 802491

Strand: Reverse

Name: aroA

Synonym: APL_0699

Alternate gene names: 126208179

Gene position: 802491-801193 (Counterclockwise)

Preceding gene: 126208180

Following gene: 126208178

Centisome position: 35.28

GC content: 43.34

Gene sequence:

>1299_bases
ATGGAAAAAATTACATTAGCACCGATCAGCCGTGTTGAGGGCGAGATTAATTTACCCGGCTCAAAAAGCCTTTCAAACCG
TGCGTTATTATTAGCCGCTTTAGCGAAAGGGACGACTAAGGTTACCAATTTATTAGACAGTGACGATATTCGCCATATGC
TCAATGCACTAAAAGCATTAGGCGTTAATTATTCATTATCGGAAGATAAAACCGTTTGTACCGTTGAAGGTGTCGGCGGT
GCGTTTAATTGGAAAAACGGTTTGGCGTTATTTTTAGGAAATGCCGGTACGGCAATGCGACCTTTAACGGCGGCACTTTG
CTTAAAAGGATCTTCGGAAGCGGAAGTAGTTTTAACCGGCGAGCCTCGTATGAAAGAACGTCCGATTAAACATTTGGTTG
ATGCGCTTCGTCAAGCCGGTGCAAGCGTTCAATATTTGGAAAATGAAGGTTATCCGCCGGTTGCAATCCGAAACAGCGGC
TTAAAAGGCGGAAAGGTGCAAATTGACGGTTCAATTTCAAGTCAATTTTTAACCGCTTTATTGATGGCGGCACCGCTTGC
CGAAGGCGATATGGAAATTGAAATTATCGGTGAACTGGTATCAAAACCTTATATTGATATTACCTTGGCGATGATGAAAG
ATTTTGGAGTAAAAGTTGAGAATCGAAATTACCAGACCTTTGTGGTTAAAGGTAATCAAAGTTATCTCTCACCGGAAAAA
TATTTGGTAGAAGGCGATGCGTCATCCGCTTCTTATTTCTTAGCAGCCGGTGCGATTAAAGGTAAAGTAAAGGTAACCGG
TATTGGTAAAAATTCGATCCAAGGCGACCGCTTGTTTGCCAATGTGCTGGAAGCGATGGGCGCGAAAATCACTTGGGGTG
ACGATTTTATCCAAGCGGAACAGGGTAAGCTTAAAGGTGTGGATATGGATATGAACCATATTCCGGATGCAGCGATGACG
ATTGCAACCGCCGCATTATTTGCCGAAGGGGAAACGGTGATTCGCAATATTTATAACTGGCGTGTCAAAGAAACCGATCG
TTTAACGGCGATGGCGACCGAATTGCGTAAAGTCGGTGCAACCGTTGAGGAAGGCGAAGATTTTATTCGCATTCAGCCTT
TGCCACTCACACAATTCCAACATGCAGAAATTGCCACTTATAATGATCACCGTATGGCGATGTGTTTTTCGTTGATTGCA
TTGTCTGATACACCGGTGACGATTTTAGATCCAAAATGTACGGCAAAAACCTTCCCGACTTATTTCACCGAATTTGAGAA
GTTGTCGGAAAGAACATAA

Upstream 100 bases:

>100_bases
GGTATGCCGAATCATTTACGTATCAGTATCGGTTTACCGCAAGAAAATGACCGCTTGTTTGCCGCATTAGTAAAAGTGTT
GGGATAATTTAGAGAATAAA

Downstream 100 bases:

>100_bases
CAAGCGATAATAAGCGGTTGAATTTGTAAAAAAATTTGCAAATCCAACCGCTTTTTTATGGACAGATACAAATTTTAATT
CAATAAAATTGCATAAATAT

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MEKITLAPISRVEGEINLPGSKSLSNRALLLAALAKGTTKVTNLLDSDDIRHMLNALKALGVNYSLSEDKTVCTVEGVGG
AFNWKNGLALFLGNAGTAMRPLTAALCLKGSSEAEVVLTGEPRMKERPIKHLVDALRQAGASVQYLENEGYPPVAIRNSG
LKGGKVQIDGSISSQFLTALLMAAPLAEGDMEIEIIGELVSKPYIDITLAMMKDFGVKVENRNYQTFVVKGNQSYLSPEK
YLVEGDASSASYFLAAGAIKGKVKVTGIGKNSIQGDRLFANVLEAMGAKITWGDDFIQAEQGKLKGVDMDMNHIPDAAMT
IATAALFAEGETVIRNIYNWRVKETDRLTAMATELRKVGATVEEGEDFIRIQPLPLTQFQHAEIATYNDHRMAMCFSLIA
LSDTPVTILDPKCTAKTFPTYFTEFEKLSERT

Sequences:

>Translated_432_residues
MEKITLAPISRVEGEINLPGSKSLSNRALLLAALAKGTTKVTNLLDSDDIRHMLNALKALGVNYSLSEDKTVCTVEGVGG
AFNWKNGLALFLGNAGTAMRPLTAALCLKGSSEAEVVLTGEPRMKERPIKHLVDALRQAGASVQYLENEGYPPVAIRNSG
LKGGKVQIDGSISSQFLTALLMAAPLAEGDMEIEIIGELVSKPYIDITLAMMKDFGVKVENRNYQTFVVKGNQSYLSPEK
YLVEGDASSASYFLAAGAIKGKVKVTGIGKNSIQGDRLFANVLEAMGAKITWGDDFIQAEQGKLKGVDMDMNHIPDAAMT
IATAALFAEGETVIRNIYNWRVKETDRLTAMATELRKVGATVEEGEDFIRIQPLPLTQFQHAEIATYNDHRMAMCFSLIA
LSDTPVTILDPKCTAKTFPTYFTEFEKLSERT
>Mature_432_residues
MEKITLAPISRVEGEINLPGSKSLSNRALLLAALAKGTTKVTNLLDSDDIRHMLNALKALGVNYSLSEDKTVCTVEGVGG
AFNWKNGLALFLGNAGTAMRPLTAALCLKGSSEAEVVLTGEPRMKERPIKHLVDALRQAGASVQYLENEGYPPVAIRNSG
LKGGKVQIDGSISSQFLTALLMAAPLAEGDMEIEIIGELVSKPYIDITLAMMKDFGVKVENRNYQTFVVKGNQSYLSPEK
YLVEGDASSASYFLAAGAIKGKVKVTGIGKNSIQGDRLFANVLEAMGAKITWGDDFIQAEQGKLKGVDMDMNHIPDAAMT
IATAALFAEGETVIRNIYNWRVKETDRLTAMATELRKVGATVEEGEDFIRIQPLPLTQFQHAEIATYNDHRMAMCFSLIA
LSDTPVTILDPKCTAKTFPTYFTEFEKLSERT

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=431, Percent_Identity=71.6937354988399, Blast_Score=605, Evalue=1e-174,
Organism=Saccharomyces cerevisiae, GI6320332, Length=443, Percent_Identity=40.8577878103837, Blast_Score=277, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_ACTP2 (A3N063)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053404.1
- STRING:   A3N063
- GeneID:   4848647
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0699
- eggNOG:   COG0128
- HOGENOM:   HBG646626
- OMA:   GADIEWG
- PhylomeDB:   A3N063
- ProtClustDB:   PRK02427
- BioCyc:   APLE416269:APL_0699-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 46911; Mature: 46911

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKITLAPISRVEGEINLPGSKSLSNRALLLAALAKGTTKVTNLLDSDDIRHMLNALKAL
CCCEEECCHHHCCCCEECCCCCCCCCHHHEEHHHHCCHHHHHHHCCCHHHHHHHHHHHHH
GVNYSLSEDKTVCTVEGVGGAFNWKNGLALFLGNAGTAMRPLTAALCLKGSSEAEVVLTG
CCCEECCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEC
EPRMKERPIKHLVDALRQAGASVQYLENEGYPPVAIRNSGLKGGKVQIDGSISSQFLTAL
CCCHHCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCCCCCEEEECCCHHHHHHHHH
LMAAPLAEGDMEIEIIGELVSKPYIDITLAMMKDFGVKVENRNYQTFVVKGNQSYLSPEK
HHHCCCCCCCEEHEEHHHHHCCCCCEEEHEEHHHCCCEEECCCEEEEEEECCCCCCCCCC
YLVEGDASSASYFLAAGAIKGKVKVTGIGKNSIQGDRLFANVLEAMGAKITWGDDFIQAE
EEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEECCHHHCCC
QGKLKGVDMDMNHIPDAAMTIATAALFAEGETVIRNIYNWRVKETDRLTAMATELRKVGA
CCCEECCCCCHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCEECCCHHHHHHHHHHHHHCC
TVEEGEDFIRIQPLPLTQFQHAEIATYNDHRMAMCFSLIALSDTPVTILDPKCTAKTFPT
CHHCCCCEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEECCCCCCCCCHH
YFTEFEKLSERT
HHHHHHHHHCCC
>Mature Secondary Structure
MEKITLAPISRVEGEINLPGSKSLSNRALLLAALAKGTTKVTNLLDSDDIRHMLNALKAL
CCCEEECCHHHCCCCEECCCCCCCCCHHHEEHHHHCCHHHHHHHCCCHHHHHHHHHHHHH
GVNYSLSEDKTVCTVEGVGGAFNWKNGLALFLGNAGTAMRPLTAALCLKGSSEAEVVLTG
CCCEECCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEC
EPRMKERPIKHLVDALRQAGASVQYLENEGYPPVAIRNSGLKGGKVQIDGSISSQFLTAL
CCCHHCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCCCCCEEEECCCHHHHHHHHH
LMAAPLAEGDMEIEIIGELVSKPYIDITLAMMKDFGVKVENRNYQTFVVKGNQSYLSPEK
HHHCCCCCCCEEHEEHHHHHCCCCCEEEHEEHHHCCCEEECCCEEEEEEECCCCCCCCCC
YLVEGDASSASYFLAAGAIKGKVKVTGIGKNSIQGDRLFANVLEAMGAKITWGDDFIQAE
EEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEECCHHHCCC
QGKLKGVDMDMNHIPDAAMTIATAALFAEGETVIRNIYNWRVKETDRLTAMATELRKVGA
CCCEECCCCCHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCEECCCHHHHHHHHHHHHHCC
TVEEGEDFIRIQPLPLTQFQHAEIATYNDHRMAMCFSLIALSDTPVTILDPKCTAKTFPT
CHHCCCCEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEECCCCCCCCCHH
YFTEFEKLSERT
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA