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The map label for this gene is hsdM3 [C]

Identifier: 126208115

GI number: 126208115

Start: 721030

End: 722595

Strand: Direct

Name: hsdM3 [C]

Synonym: APL_0633

Alternate gene names: 126208115

Gene position: 721030-722595 (Clockwise)

Preceding gene: 126208114

Following gene: 126208116

Centisome position: 31.7

GC content: 45.4

Gene sequence:

>1566_bases
TTGGAAAAAGCTGCCGACAAACTCCGCCAGCAATTAGACGCAGCCAACTACAAGCATATTGTGCTTGGTTTAATTTTCCT
CAAATACGTTTCTGATAGTTTCACCGCACAGCGTGAACGCCTTTCTGCACAATTCCGAGATCCAAATTCTGACTACTATT
TGTCAGATATAACCACCGAAGAAATCGAATCTGAACTCGAAGAACGGGATTACTACACCCAAGATAACGTGTTTTGGGTG
CCTCAAATCGCCCGTTGGAACGAAATCAAAGCCGTAGTACGAGCTAATTTAGGTGACACCATTTTTGAGAACAAAACCTT
TAAAGGCGTGGCAAATTTGATTGATGAAGCCTTTGACGCTATTGAAAAAGACAACCCAAAATTGAAAGGTGTGATTCAAC
GTATTTCGAGCTACAACGTGGACGAAAGTATTTTAATCGGCTTAGTGGAATTGTTTTCTGACACCAACTTCACTCACCCA
ACGCTTAACGGCAAGCCTGTTTCTTTGGCAGCAAAAGATATTTTAGGACATGTTTACGAGTATTTTCTCGGACAATTTGC
TCTCGCAGAAGGCAAGAAAGGCGGGCAGTATTTCACGCCAAAATCTATTGTTACCCTGATCATCGAAATGCTCGAACCCT
ACGAAGGGCGAATTTACGACCCTGCAATGGGATCAGGCGGTTTCTTCGTGCAAACCGAACGTTTTATCCGTGAACATCAG
GGCAACCCAAACCGTGTGTCGATTTATGGGCAAGAATTTAACCCAACCACTTGGAAACTGGCTGCAATGAATATGGCAAT
TCGTGGCTTGTCGTTTGATTTTGGCAAGGGCAATGCCGACACGTTTAGCAATCCACAACATCTCGATAAACGAATGGATT
TTGTGATGGCGAACCCACCGTTCAATATGAACGAATGGTGGAATCAAAGCCTTGCCAACGATCCACGCTGGAAATTTGGC
ACGCCACCGTCTAGCAACGCCAACTTTGCGTGGTTGCAACATATGATTTACCACCTTTCCGAAAAAGGCAAAATGGCGTT
ATTGCTTGCCAACGGCTCAATGTCGAGCAACACCAACAACGAGGGCGAAATCCGCCGTAACATCGTACGAGCCGATTTGG
TGGAAGCGATGATTGCCTTGCCAAGCCAATTATTTACCAATACACAAATCCCAGCTTGCATTTGGGTGCTGAACAAAGCC
AAACCACGCAAGGGCGAAGTGCTGTTTATTGATGCTCGCCAACTTGGTTATATGAAAACCCGTGTAATGCGTGATTTCAC
GCCAAAAGACATTGCAAGCGTAGCAGAAACCTACCACCAATGGCAAAAAGGAGAAGGTTATCAAGACGTGCCAGCATTCT
GCAAATCCGCCACCCTTGCCGAGATCGAAAGCCACGATTTTGTGCTAACCCCGGGGCGATACGTTGGAGCGGCTGAGGTG
GAAGATGACGGCATTCCATTTGCAGAAAAAATGCAAAATCTGACCGCACTTTTAAACGAACAATTCAAACAAGGGGCGGA
ATTAGAAGCAAAAATCCGAGCGAATTTGGCAACGCTCGGGTTTTAA

Upstream 100 bases:

>100_bases
TTATTGACTTTATAATATAGGATTTTATCATTATGACAACAATCAATACTGAACTTCTAGCCGCTCAACAATCCTTTCTC
AACGATCTCGACGATCGTCT

Downstream 100 bases:

>100_bases
ACGTAGGGACGCACTGCGTGCGTATTTACGGCATTTACAACCACACCAACCCCATTACGCCATAAAAATCCCAACAACGG
ACGCACGCAGTGCGTCCCTA

Product: putative type I modification enzyme

Products: NA

Alternate protein names: M.HindVIIP [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MEKAADKLRQQLDAANYKHIVLGLIFLKYVSDSFTAQRERLSAQFRDPNSDYYLSDITTEEIESELEERDYYTQDNVFWV
PQIARWNEIKAVVRANLGDTIFENKTFKGVANLIDEAFDAIEKDNPKLKGVIQRISSYNVDESILIGLVELFSDTNFTHP
TLNGKPVSLAAKDILGHVYEYFLGQFALAEGKKGGQYFTPKSIVTLIIEMLEPYEGRIYDPAMGSGGFFVQTERFIREHQ
GNPNRVSIYGQEFNPTTWKLAAMNMAIRGLSFDFGKGNADTFSNPQHLDKRMDFVMANPPFNMNEWWNQSLANDPRWKFG
TPPSSNANFAWLQHMIYHLSEKGKMALLLANGSMSSNTNNEGEIRRNIVRADLVEAMIALPSQLFTNTQIPACIWVLNKA
KPRKGEVLFIDARQLGYMKTRVMRDFTPKDIASVAETYHQWQKGEGYQDVPAFCKSATLAEIESHDFVLTPGRYVGAAEV
EDDGIPFAEKMQNLTALLNEQFKQGAELEAKIRANLATLGF

Sequences:

>Translated_521_residues
MEKAADKLRQQLDAANYKHIVLGLIFLKYVSDSFTAQRERLSAQFRDPNSDYYLSDITTEEIESELEERDYYTQDNVFWV
PQIARWNEIKAVVRANLGDTIFENKTFKGVANLIDEAFDAIEKDNPKLKGVIQRISSYNVDESILIGLVELFSDTNFTHP
TLNGKPVSLAAKDILGHVYEYFLGQFALAEGKKGGQYFTPKSIVTLIIEMLEPYEGRIYDPAMGSGGFFVQTERFIREHQ
GNPNRVSIYGQEFNPTTWKLAAMNMAIRGLSFDFGKGNADTFSNPQHLDKRMDFVMANPPFNMNEWWNQSLANDPRWKFG
TPPSSNANFAWLQHMIYHLSEKGKMALLLANGSMSSNTNNEGEIRRNIVRADLVEAMIALPSQLFTNTQIPACIWVLNKA
KPRKGEVLFIDARQLGYMKTRVMRDFTPKDIASVAETYHQWQKGEGYQDVPAFCKSATLAEIESHDFVLTPGRYVGAAEV
EDDGIPFAEKMQNLTALLNEQFKQGAELEAKIRANLATLGF
>Mature_521_residues
MEKAADKLRQQLDAANYKHIVLGLIFLKYVSDSFTAQRERLSAQFRDPNSDYYLSDITTEEIESELEERDYYTQDNVFWV
PQIARWNEIKAVVRANLGDTIFENKTFKGVANLIDEAFDAIEKDNPKLKGVIQRISSYNVDESILIGLVELFSDTNFTHP
TLNGKPVSLAAKDILGHVYEYFLGQFALAEGKKGGQYFTPKSIVTLIIEMLEPYEGRIYDPAMGSGGFFVQTERFIREHQ
GNPNRVSIYGQEFNPTTWKLAAMNMAIRGLSFDFGKGNADTFSNPQHLDKRMDFVMANPPFNMNEWWNQSLANDPRWKFG
TPPSSNANFAWLQHMIYHLSEKGKMALLLANGSMSSNTNNEGEIRRNIVRADLVEAMIALPSQLFTNTQIPACIWVLNKA
KPRKGEVLFIDARQLGYMKTRVMRDFTPKDIASVAETYHQWQKGEGYQDVPAFCKSATLAEIESHDFVLTPGRYVGAAEV
EDDGIPFAEKMQNLTALLNEQFKQGAELEAKIRANLATLGF

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=231, Percent_Identity=25.5411255411255, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003356
- InterPro:   IPR002296 [H]

Pfam domain/function: PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 59067; Mature: 59067

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKAADKLRQQLDAANYKHIVLGLIFLKYVSDSFTAQRERLSAQFRDPNSDYYLSDITTE
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHH
EIESELEERDYYTQDNVFWVPQIARWNEIKAVVRANLGDTIFENKTFKGVANLIDEAFDA
HHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHH
IEKDNPKLKGVIQRISSYNVDESILIGLVELFSDTNFTHPTLNGKPVSLAAKDILGHVYE
HHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
YFLGQFALAEGKKGGQYFTPKSIVTLIIEMLEPYEGRIYDPAMGSGGFFVQTERFIREHQ
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEHHHHHHHHC
GNPNRVSIYGQEFNPTTWKLAAMNMAIRGLSFDFGKGNADTFSNPQHLDKRMDFVMANPP
CCCCEEEEEECCCCCCEEEHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHCEEECCCC
FNMNEWWNQSLANDPRWKFGTPPSSNANFAWLQHMIYHLSEKGKMALLLANGSMSSNTNN
CCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
EGEIRRNIVRADLVEAMIALPSQLFTNTQIPACIWVLNKAKPRKGEVLFIDARQLGYMKT
CHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHH
RVMRDFTPKDIASVAETYHQWQKGEGYQDVPAFCKSATLAEIESHDFVLTPGRYVGAAEV
HHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHCCCCEEECCCCEECCCCC
EDDGIPFAEKMQNLTALLNEQFKQGAELEAKIRANLATLGF
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCC
>Mature Secondary Structure
MEKAADKLRQQLDAANYKHIVLGLIFLKYVSDSFTAQRERLSAQFRDPNSDYYLSDITTE
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHH
EIESELEERDYYTQDNVFWVPQIARWNEIKAVVRANLGDTIFENKTFKGVANLIDEAFDA
HHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHH
IEKDNPKLKGVIQRISSYNVDESILIGLVELFSDTNFTHPTLNGKPVSLAAKDILGHVYE
HHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
YFLGQFALAEGKKGGQYFTPKSIVTLIIEMLEPYEGRIYDPAMGSGGFFVQTERFIREHQ
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEHHHHHHHHC
GNPNRVSIYGQEFNPTTWKLAAMNMAIRGLSFDFGKGNADTFSNPQHLDKRMDFVMANPP
CCCCEEEEEECCCCCCEEEHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHCEEECCCC
FNMNEWWNQSLANDPRWKFGTPPSSNANFAWLQHMIYHLSEKGKMALLLANGSMSSNTNN
CCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
EGEIRRNIVRADLVEAMIALPSQLFTNTQIPACIWVLNKAKPRKGEVLFIDARQLGYMKT
CHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHH
RVMRDFTPKDIASVAETYHQWQKGEGYQDVPAFCKSATLAEIESHDFVLTPGRYVGAAEV
HHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHCCCCEEECCCCEECCCCC
EDDGIPFAEKMQNLTALLNEQFKQGAELEAKIRANLATLGF
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]