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The map label for this gene is thiE [H]

Identifier: 126208022

GI number: 126208022

Start: 610464

End: 610877

Strand: Direct

Name: thiE [H]

Synonym: APL_0540

Alternate gene names: 126208022

Gene position: 610464-610877 (Clockwise)

Preceding gene: 126208021

Following gene: 126208023

Centisome position: 26.84

GC content: 44.2

Gene sequence:

>414_bases
GTGGACGATAACGTGGCATTAGCGATTGAGATTGATGCGGACGGCGTACATGTCGGACAAAAAGATATGTCACCGATAAT
GATTCGTCAGATGACCGATAAACCTTTGATTATCGGCTTATCGAATAATACCTTAGAAGATCTTTGGCGTTCAGAACAGA
TGATCGAGGTGGATTATTGCGGATTAGGTCCGGTCTTCCCGACCAATTCCAAAGAAAAACATAATCCGCCGATTGGTTTG
GATTTTGTTAAAAAAGCCCGTGAAGCTGGGATTCGTAAGCCGATTGTTTCTATCGGCGGAGTGAAAGCCGAACACGTGGC
AACCTTGAAACAAAACGGAGTGGACGGCATCGCGGTTATCACTGCGATTAGTTTAGCTTCTGATGTTTCTCAAGCCGTCA
AACGATTATTATAA

Upstream 100 bases:

>100_bases
ACGGTAGTGATTAAGGGCGGGCATTTGGCGGATTCGCAAAGTGCGCATTGCCGTGATTGGGTATTTACACCAATGCCGTC
TATATAACGTTCCGTTTATT

Downstream 100 bases:

>100_bases
TTTTGCTCTTATACAAGCGGTCTTTTTTCAAGATTTTTTGCAAAATTTCTTGGAAAAACGACCGCTTGCTTTTTTGTGCT
TTTCCTTTCCAAAATAGCGG

Product: thiamine monophosphate synthase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MDDNVALAIEIDADGVHVGQKDMSPIMIRQMTDKPLIIGLSNNTLEDLWRSEQMIEVDYCGLGPVFPTNSKEKHNPPIGL
DFVKKAREAGIRKPIVSIGGVKAEHVATLKQNGVDGIAVITAISLASDVSQAVKRLL

Sequences:

>Translated_137_residues
MDDNVALAIEIDADGVHVGQKDMSPIMIRQMTDKPLIIGLSNNTLEDLWRSEQMIEVDYCGLGPVFPTNSKEKHNPPIGL
DFVKKAREAGIRKPIVSIGGVKAEHVATLKQNGVDGIAVITAISLASDVSQAVKRLL
>Mature_137_residues
MDDNVALAIEIDADGVHVGQKDMSPIMIRQMTDKPLIIGLSNNTLEDLWRSEQMIEVDYCGLGPVFPTNSKEKHNPPIGL
DFVKKAREAGIRKPIVSIGGVKAEHVATLKQNGVDGIAVITAISLASDVSQAVKRLL

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=145, Percent_Identity=32.4137931034483, Blast_Score=64, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325042, Length=159, Percent_Identity=34.5911949685535, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 14799; Mature: 14799

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDNVALAIEIDADGVHVGQKDMSPIMIRQMTDKPLIIGLSNNTLEDLWRSEQMIEVDYC
CCCCEEEEEEECCCCEEECCCCCCHHEEEECCCCCEEEEECCCCHHHHHCCCCEEEEECC
GLGPVFPTNSKEKHNPPIGLDFVKKAREAGIRKPIVSIGGVKAEHVATLKQNGVDGIAVI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHCCCCHHHHH
TAISLASDVSQAVKRLL
HHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDDNVALAIEIDADGVHVGQKDMSPIMIRQMTDKPLIIGLSNNTLEDLWRSEQMIEVDYC
CCCCEEEEEEECCCCEEECCCCCCHHEEEECCCCCEEEEECCCCHHHHHCCCCEEEEECC
GLGPVFPTNSKEKHNPPIGLDFVKKAREAGIRKPIVSIGGVKAEHVATLKQNGVDGIAVI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHCCCCHHHHH
TAISLASDVSQAVKRLL
HHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA