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The map label for this gene is nagK [H]

Identifier: 126207900

GI number: 126207900

Start: 473935

End: 474852

Strand: Reverse

Name: nagK [H]

Synonym: APL_0416

Alternate gene names: 126207900

Gene position: 474852-473935 (Counterclockwise)

Preceding gene: 126207901

Following gene: 126207899

Centisome position: 20.88

GC content: 44.44

Gene sequence:

>918_bases
ATGGCTGTTTATTACGGTTTAGATGTAGGCGGTACGAAAATTGAGTTGGCGGCATTTAATGCTCAACTGGAAAAACTGTA
TAGCGAGCGAGTGCCAACACCGCAAACCAATTATGAAGATTGGTTGAATACGGTGGAAACCTTAGTGCTTAACGCCGATC
AAAAATTCGGTGAGCAAGGTTCCGTCGGTTTAGGCTTACCCGGTTTCGTAAACCGTGAAACCGGTATTGCGGAAATCACC
AATATTCGTGTTGCCGACGGTAAAGCGATTTTAAAAGATTTAAGCGAGCGTTTAGGACGTGAAGTACGCGCGGAAAACGA
TGCAAACTGCTTTGCCCTTTCGGAAGCGTGGGATCCGAATAACCAACAATTCTCAACCGTGCTCGGTTTAATTATTGGCA
CCGGTTTCGGCGGCGGCTTTGTGTTTGACGGAAAAATTCATTCCGGCAGAATCGGTATGGCGGGCGAAGTCGGTCATATG
CAACTCAATTATCACGCTTTAAAACTATTAGGTTGGGATAAGGCGCCGATTTATGATTGCGGTTGTGGGAACCGAGCTTG
TTTAGATACTTATATTTCCGGTCGCGGTTTCGAGATGCTATTTAGAGATTTGGTCGGTGAAACGATTTCGGCGAAAGAAA
TTATCGAACGTTTCTATCAAAAAGAGTCGAAAACTATCGAATTTGTGGATAAATACATCGAATTAATGGCAATCAGTATC
GGTAATCTGATCACGATTCTTGATCCGGATATGATCGTATTCGGCGGCGGTTTATCTAATTTTGATCATATTTATGAGGC
ATTGCCAAAAGCACTTCCTCCGCATTTAATGCGTAGTGCCAAAGTGCCGGTCTTTAAAAAAGCGATTCACGGCGACTCGG
GCGGTACTCGTGGTGCGGCGGCACTCTTCTTAAAATAA

Upstream 100 bases:

>100_bases
TTGTCAGTACGTTAATTGCTTGGTTATTCCACATTATCGTATTTGTTTGGGTAATTCCTTTCCTAGAAACCTTTAAAATC
TTTCAAAAACGAGGTTAATT

Downstream 100 bases:

>100_bases
TTTGTTTTTACCGAATACATGGGACTTCCCGTGTATTCTTTTTATATAGGAAAGTATTATGCTAAACATTACTCCGATTC
CGGCGCTCTCCGATAATTAC

Product: N-acetyl-D-glucosamine kinase

Products: NA

Alternate protein names: GlcNAc kinase [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MAVYYGLDVGGTKIELAAFNAQLEKLYSERVPTPQTNYEDWLNTVETLVLNADQKFGEQGSVGLGLPGFVNRETGIAEIT
NIRVADGKAILKDLSERLGREVRAENDANCFALSEAWDPNNQQFSTVLGLIIGTGFGGGFVFDGKIHSGRIGMAGEVGHM
QLNYHALKLLGWDKAPIYDCGCGNRACLDTYISGRGFEMLFRDLVGETISAKEIIERFYQKESKTIEFVDKYIELMAISI
GNLITILDPDMIVFGGGLSNFDHIYEALPKALPPHLMRSAKVPVFKKAIHGDSGGTRGAAALFLK

Sequences:

>Translated_305_residues
MAVYYGLDVGGTKIELAAFNAQLEKLYSERVPTPQTNYEDWLNTVETLVLNADQKFGEQGSVGLGLPGFVNRETGIAEIT
NIRVADGKAILKDLSERLGREVRAENDANCFALSEAWDPNNQQFSTVLGLIIGTGFGGGFVFDGKIHSGRIGMAGEVGHM
QLNYHALKLLGWDKAPIYDCGCGNRACLDTYISGRGFEMLFRDLVGETISAKEIIERFYQKESKTIEFVDKYIELMAISI
GNLITILDPDMIVFGGGLSNFDHIYEALPKALPPHLMRSAKVPVFKKAIHGDSGGTRGAAALFLK
>Mature_304_residues
AVYYGLDVGGTKIELAAFNAQLEKLYSERVPTPQTNYEDWLNTVETLVLNADQKFGEQGSVGLGLPGFVNRETGIAEITN
IRVADGKAILKDLSERLGREVRAENDANCFALSEAWDPNNQQFSTVLGLIIGTGFGGGFVFDGKIHSGRIGMAGEVGHMQ
LNYHALKLLGWDKAPIYDCGCGNRACLDTYISGRGFEMLFRDLVGETISAKEIIERFYQKESKTIEFVDKYIELMAISIG
NLITILDPDMIVFGGGLSNFDHIYEALPKALPPHLMRSAKVPVFKKAIHGDSGGTRGAAALFLK

Specific function: Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family. NagK subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787363, Length=302, Percent_Identity=47.3509933774834, Blast_Score=294, Evalue=4e-81,
Organism=Escherichia coli, GI87081733, Length=300, Percent_Identity=41.3333333333333, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1790522, Length=313, Percent_Identity=25.5591054313099, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786891, Length=277, Percent_Identity=23.1046931407942, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.59 [H]

Molecular weight: Translated: 33363; Mature: 33231

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS01125 ROK ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVYYGLDVGGTKIELAAFNAQLEKLYSERVPTPQTNYEDWLNTVETLVLNADQKFGEQG
CEEEEEECCCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHCCCCC
SVGLGLPGFVNRETGIAEITNIRVADGKAILKDLSERLGREVRAENDANCFALSEAWDPN
CCCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
NQQFSTVLGLIIGTGFGGGFVFDGKIHSGRIGMAGEVGHMQLNYHALKLLGWDKAPIYDC
CHHHHHHHHHHHHCCCCCCEEECCEEECCCCCCCCCCCEEEECCEEEEEECCCCCCCEEC
GCGNRACLDTYISGRGFEMLFRDLVGETISAKEIIERFYQKESKTIEFVDKYIELMAISI
CCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GNLITILDPDMIVFGGGLSNFDHIYEALPKALPPHLMRSAKVPVFKKAIHGDSGGTRGAA
CCEEEEECCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHCCCCHHHHHHCCCCCCCCCCE
ALFLK
EEEEC
>Mature Secondary Structure 
AVYYGLDVGGTKIELAAFNAQLEKLYSERVPTPQTNYEDWLNTVETLVLNADQKFGEQG
EEEEEECCCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHCCCCC
SVGLGLPGFVNRETGIAEITNIRVADGKAILKDLSERLGREVRAENDANCFALSEAWDPN
CCCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
NQQFSTVLGLIIGTGFGGGFVFDGKIHSGRIGMAGEVGHMQLNYHALKLLGWDKAPIYDC
CHHHHHHHHHHHHCCCCCCEEECCEEECCCCCCCCCCCEEEECCEEEEEECCCCCCCEEC
GCGNRACLDTYISGRGFEMLFRDLVGETISAKEIIERFYQKESKTIEFVDKYIELMAISI
CCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GNLITILDPDMIVFGGGLSNFDHIYEALPKALPPHLMRSAKVPVFKKAIHGDSGGTRGAA
CCEEEEECCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHCCCCHHHHHHCCCCCCCCCCE
ALFLK
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA