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The map label for this gene is gloB

Identifier: 126207899

GI number: 126207899

Start: 473169

End: 473876

Strand: Reverse

Name: gloB

Synonym: APL_0415

Alternate gene names: 126207899

Gene position: 473876-473169 (Counterclockwise)

Preceding gene: 126207900

Following gene: 126207898

Centisome position: 20.83

GC content: 43.22

Gene sequence:

>708_bases
ATGCTAAACATTACTCCGATTCCGGCGCTCTCCGATAATTACATTTGGGCGATTCAAAAAGATAATGACGTGATTATTGT
CGATCCGTCAGATGCCGTTCCCGTATTGGCGTTTATTGCAAAAAATCAGCTAAATTTGACCGCTATTTTATTAACGCATA
ATCATCATGATCACACCGACGGTATGCCGGAATTACTCTCACGTTATCCGCAATTATCGGTTTACGGCCCGCAAGAAGTC
GCACAATTTGCTAATCGGATTGTGCAACCTGAAGATCATCTCACTTTATTTGATTATGATGTCCGAGTGATTGAAAGTGC
CGGTCATACCGCTCAGCACGTCAGCTATTTATTCGGTAACGAATACCTATTCTGTGGTGATGCGTTATTCTCCGGCGGCT
GCGGTCGTGTCTTTACCGGTAATTATCAAGCGCAATTTGATGCGTTGCAACGTTTTAAAGCGTTACCGGAATTTGTCGAA
ATCTTCCCTGCGCATGAATATACGTTAAGCAATCTAAAATTCGCCGAAGCGGTTTTAGCGCCGAGCTGCGCTCTGTTTGA
AATTCAAGAACGTGCGGAAATCTTAAGATCCCGCAATCAGCCTACACTTCCGACAACGTTGGAAAGAGAGCTGCAAATCA
ACCCGTTCTTACAAGCGGTCGATTTAGATCAATTTATTGCATTACGCCACCAAAAAGATAATTTCTAA

Upstream 100 bases:

>100_bases
TCGGGCGGTACTCGTGGTGCGGCGGCACTCTTCTTAAAATAATTTGTTTTTACCGAATACATGGGACTTCCCGTGTATTC
TTTTTATATAGGAAAGTATT

Downstream 100 bases:

>100_bases
TCCATAATAAACGGGAAATAAAGCTTTCCCGTTTATTTCTCCTCCTGCCAAACTTTTTATTTTTTATACTGTCAATTCCT
CCTGTATCTATCTTAGAATC

Product: putative hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MLNITPIPALSDNYIWAIQKDNDVIIVDPSDAVPVLAFIAKNQLNLTAILLTHNHHDHTDGMPELLSRYPQLSVYGPQEV
AQFANRIVQPEDHLTLFDYDVRVIESAGHTAQHVSYLFGNEYLFCGDALFSGGCGRVFTGNYQAQFDALQRFKALPEFVE
IFPAHEYTLSNLKFAEAVLAPSCALFEIQERAEILRSRNQPTLPTTLERELQINPFLQAVDLDQFIALRHQKDNF

Sequences:

>Translated_235_residues
MLNITPIPALSDNYIWAIQKDNDVIIVDPSDAVPVLAFIAKNQLNLTAILLTHNHHDHTDGMPELLSRYPQLSVYGPQEV
AQFANRIVQPEDHLTLFDYDVRVIESAGHTAQHVSYLFGNEYLFCGDALFSGGCGRVFTGNYQAQFDALQRFKALPEFVE
IFPAHEYTLSNLKFAEAVLAPSCALFEIQERAEILRSRNQPTLPTTLERELQINPFLQAVDLDQFIALRHQKDNF
>Mature_235_residues
MLNITPIPALSDNYIWAIQKDNDVIIVDPSDAVPVLAFIAKNQLNLTAILLTHNHHDHTDGMPELLSRYPQLSVYGPQEV
AQFANRIVQPEDHLTLFDYDVRVIESAGHTAQHVSYLFGNEYLFCGDALFSGGCGRVFTGNYQAQFDALQRFKALPEFVE
IFPAHEYTLSNLKFAEAVLAPSCALFEIQERAEILRSRNQPTLPTTLERELQINPFLQAVDLDQFIALRHQKDNF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI21703352, Length=226, Percent_Identity=34.9557522123894, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI116642887, Length=226, Percent_Identity=34.9557522123894, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI14150041, Length=232, Percent_Identity=32.7586206896552, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI94538322, Length=256, Percent_Identity=30.078125, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI94538320, Length=256, Percent_Identity=30.078125, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI46361987, Length=186, Percent_Identity=34.4086021505376, Blast_Score=112, Evalue=2e-25,
Organism=Escherichia coli, GI1786406, Length=252, Percent_Identity=41.2698412698413, Blast_Score=182, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17536925, Length=237, Percent_Identity=27.0042194092827, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6320478, Length=229, Percent_Identity=26.6375545851528, Blast_Score=79, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324614, Length=228, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356335, Length=227, Percent_Identity=34.3612334801762, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24667711, Length=227, Percent_Identity=34.3612334801762, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24667703, Length=227, Percent_Identity=34.3612334801762, Blast_Score=139, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_ACTP2 (A3MZD3)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053124.1
- ProteinModelPortal:   A3MZD3
- SMR:   A3MZD3
- STRING:   A3MZD3
- GeneID:   4848668
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0415
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- PhylomeDB:   A3MZD3
- ProtClustDB:   CLSK918914
- BioCyc:   APLE416269:APL_0415-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 26614; Mature: 26614

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNITPIPALSDNYIWAIQKDNDVIIVDPSDAVPVLAFIAKNQLNLTAILLTHNHHDHTD
CCCCCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCC
GMPELLSRYPQLSVYGPQEVAQFANRIVQPEDHLTLFDYDVRVIESAGHTAQHVSYLFGN
CHHHHHHHCCCEEEECHHHHHHHHHHHCCCCCCEEEEECCEEEEECCCCHHHHHHHHHCC
EYLFCGDALFSGGCGRVFTGNYQAQFDALQRFKALPEFVEIFPAHEYTLSNLKFAEAVLA
CEEEECHHHHCCCCCCEEECCCHHHHHHHHHHHHCHHHHHHHCCCCHHHHCCHHHHHHHC
PSCALFEIQERAEILRSRNQPTLPTTLERELQINPFLQAVDLDQFIALRHQKDNF
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHCEECCCHHHHHCHHHHHHHHHCCCCC
>Mature Secondary Structure
MLNITPIPALSDNYIWAIQKDNDVIIVDPSDAVPVLAFIAKNQLNLTAILLTHNHHDHTD
CCCCCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCC
GMPELLSRYPQLSVYGPQEVAQFANRIVQPEDHLTLFDYDVRVIESAGHTAQHVSYLFGN
CHHHHHHHCCCEEEECHHHHHHHHHHHCCCCCCEEEEECCEEEEECCCCHHHHHHHHHCC
EYLFCGDALFSGGCGRVFTGNYQAQFDALQRFKALPEFVEIFPAHEYTLSNLKFAEAVLA
CEEEECHHHHCCCCCCEEECCCHHHHHHHHHHHHCHHHHHHHCCCCHHHHCCHHHHHHHC
PSCALFEIQERAEILRSRNQPTLPTTLERELQINPFLQAVDLDQFIALRHQKDNF
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHCEECCCHHHHHCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA