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The map label for this gene is tnsD [H]

Identifier: 126176538

GI number: 126176538

Start: 5097427

End: 5098929

Strand: Reverse

Name: tnsD [H]

Synonym: Sbal_4357

Alternate gene names: 126176538

Gene position: 5098929-5097427 (Counterclockwise)

Preceding gene: 126176539

Following gene: 126176537

Centisome position: 99.45

GC content: 40.05

Gene sequence:

>1503_bases
ATGCTTGGATTTCCGATACCTTATTCTGAGGAGCTACTTTATAGCACAATAGCAAGATCTGGTGTTCATGATGGTGATGT
ATCACCTAAGCAGTTATTGGAAAAGGTGTTTAATAACCGTAGGGTGATTGCAACAGTCGATTTGCCTAGTCATGTGCAAG
CCGTAGCAAATCAGTACCCTGATTCGCTAGGGATGGATGCTAAAACTTTAATATATCGCCATACACTTTGGCCCATTTAT
GCACCTTTTTTACCACCTGATCGAAGTGATAAGGTAATAAAATGGATGAGCGGAAGTTCCCAGGGGGCTGCGCATTTAGC
AAGTGGTATTGTAGCTTCGAGAGTAAAGACAAAGGGCAGACTTTATGTTTGCGTAGAGTGTTTAAAAGAACAAAGAATAA
AATACGGTGAATGTTTTTGGGAACGTTTTTGGCAAATTCCTATAGTAAATACATGTCCAAAACATGGACCTTTGAATAAG
ACTACTATCGTCATCGATGGTGAACACCGGCATACCTTCACAGTAATAGAGGACGCTGAAGTACTAGGTAAAGTTAACGC
AACTAATGTCGACAAGTTATTTTCGCATCAAGTCTCTCAATTAATGCAGGTTATCAGTCAAGGAATAAGCCCCACTCAAT
GGACAGTATTTTATGAGCAATTTGCATCAAACCTGGGTTACTTAAATGGTCTAAGGATTGATCATTCTAGGATTTATGAT
TCCGTAATAAGGTTTTGGGGTAAAGGTTGGCTTCAGAAAGCCGGTATTCTACCTTCTTATATTGAAACTTCTTGGCTTAA
AGCGTTGTTTCGTAAACACAGAAAAAGCTTTAGTTTTGCGGAGCATATAGTGGTAATCACAGCTCTTTCAAATGGAAGGT
TCAGTATCTGTGAGGCAATTGATAAAGCCTCGAGTATAGTAATAAAAAGCGAAAACAATATTCCCCCCAAAAAGCGGGAT
CTAAACACTTTAAACATGCTTAGCCCAGACCAAATAGATTGGGAGGTGCTAATTGAAGCTGTACCACCAAAGGCTGCCCG
CCTGCTAAATAAAGCGCTCTACGCAAGGCTTTATCGAAACCACTACGATTGGCTAATGCACATCAATAGTTTATTTCATG
CTGAAAAAGTAGTGGTCAATAACCGCGTCGACTGGCCACAACGAGATAGACAAATTGCTCAAAATCTTCGTCGTGTTTGT
AAAAATCTATCTGAAGATTTAAAAGCACCACGCCTGAGCAGAACATTCCTAATTCACCAACTTGAAAATAGAGCAACGGT
CGAAAAAAATTTGCATCGGCTACCTCGTAGCAGCCATTTACTGGCTCTTTATTCTGAAAGTACGGATGAATATCAAGCAA
GAAGGCTGACTCGCACCTACCTCGATATACTGAAAAATAAGAAACATATTAAACGATGGTCGCTATTGCGTGCAGCAGGC
TTAAGTGATGAGCGGATGACAGATATTGTGAGTAAGCTTTTAGAGGAGATTTTGAGTGAGTAA

Upstream 100 bases:

>100_bases
ATCAATATGCCTATTGTAAAGAAGAAAGACTATCACTGGTAGAGAGGCTTGAGCAAAATGGCCAGATGTTTGATCTTGAT
GGCTGGGCGGCTGGCTTCTA

Downstream 100 bases:

>100_bases
GAGTGAAGTAAAATTCCAAAAATTAAACGATGATGCAAAGATATTAGGCATTGGAGGGTTGTTTCGAAGTGTCGGTAATA
AGTCTTGGGCCATTAACCTA

Product: transposition protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MLGFPIPYSEELLYSTIARSGVHDGDVSPKQLLEKVFNNRRVIATVDLPSHVQAVANQYPDSLGMDAKTLIYRHTLWPIY
APFLPPDRSDKVIKWMSGSSQGAAHLASGIVASRVKTKGRLYVCVECLKEQRIKYGECFWERFWQIPIVNTCPKHGPLNK
TTIVIDGEHRHTFTVIEDAEVLGKVNATNVDKLFSHQVSQLMQVISQGISPTQWTVFYEQFASNLGYLNGLRIDHSRIYD
SVIRFWGKGWLQKAGILPSYIETSWLKALFRKHRKSFSFAEHIVVITALSNGRFSICEAIDKASSIVIKSENNIPPKKRD
LNTLNMLSPDQIDWEVLIEAVPPKAARLLNKALYARLYRNHYDWLMHINSLFHAEKVVVNNRVDWPQRDRQIAQNLRRVC
KNLSEDLKAPRLSRTFLIHQLENRATVEKNLHRLPRSSHLLALYSESTDEYQARRLTRTYLDILKNKKHIKRWSLLRAAG
LSDERMTDIVSKLLEEILSE

Sequences:

>Translated_500_residues
MLGFPIPYSEELLYSTIARSGVHDGDVSPKQLLEKVFNNRRVIATVDLPSHVQAVANQYPDSLGMDAKTLIYRHTLWPIY
APFLPPDRSDKVIKWMSGSSQGAAHLASGIVASRVKTKGRLYVCVECLKEQRIKYGECFWERFWQIPIVNTCPKHGPLNK
TTIVIDGEHRHTFTVIEDAEVLGKVNATNVDKLFSHQVSQLMQVISQGISPTQWTVFYEQFASNLGYLNGLRIDHSRIYD
SVIRFWGKGWLQKAGILPSYIETSWLKALFRKHRKSFSFAEHIVVITALSNGRFSICEAIDKASSIVIKSENNIPPKKRD
LNTLNMLSPDQIDWEVLIEAVPPKAARLLNKALYARLYRNHYDWLMHINSLFHAEKVVVNNRVDWPQRDRQIAQNLRRVC
KNLSEDLKAPRLSRTFLIHQLENRATVEKNLHRLPRSSHLLALYSESTDEYQARRLTRTYLDILKNKKHIKRWSLLRAAG
LSDERMTDIVSKLLEEILSE
>Mature_500_residues
MLGFPIPYSEELLYSTIARSGVHDGDVSPKQLLEKVFNNRRVIATVDLPSHVQAVANQYPDSLGMDAKTLIYRHTLWPIY
APFLPPDRSDKVIKWMSGSSQGAAHLASGIVASRVKTKGRLYVCVECLKEQRIKYGECFWERFWQIPIVNTCPKHGPLNK
TTIVIDGEHRHTFTVIEDAEVLGKVNATNVDKLFSHQVSQLMQVISQGISPTQWTVFYEQFASNLGYLNGLRIDHSRIYD
SVIRFWGKGWLQKAGILPSYIETSWLKALFRKHRKSFSFAEHIVVITALSNGRFSICEAIDKASSIVIKSENNIPPKKRD
LNTLNMLSPDQIDWEVLIEAVPPKAARLLNKALYARLYRNHYDWLMHINSLFHAEKVVVNNRVDWPQRDRQIAQNLRRVC
KNLSEDLKAPRLSRTFLIHQLENRATVEKNLHRLPRSSHLLALYSESTDEYQARRLTRTYLDILKNKKHIKRWSLLRAAG
LSDERMTDIVSKLLEEILSE

Specific function: TnsABC + tnsD promote high-frequency insertion of Tn7 into a specific target site known as att-Tn7 whereas tnsABC + tnsE promote low-frequency insertion into many different sites [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 57589; Mature: 57589

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGFPIPYSEELLYSTIARSGVHDGDVSPKQLLEKVFNNRRVIATVDLPSHVQAVANQYP
CCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCC
DSLGMDAKTLIYRHTLWPIYAPFLPPDRSDKVIKWMSGSSQGAAHLASGIVASRVKTKGR
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCC
LYVCVECLKEQRIKYGECFWERFWQIPIVNTCPKHGPLNKTTIVIDGEHRHTFTVIEDAE
HHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECHH
VLGKVNATNVDKLFSHQVSQLMQVISQGISPTQWTVFYEQFASNLGYLNGLRIDHSRIYD
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
SVIRFWGKGWLQKAGILPSYIETSWLKALFRKHRKSFSFAEHIVVITALSNGRFSICEAI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHCEEEEEEEECCCCHHHHHHH
DKASSIVIKSENNIPPKKRDLNTLNMLSPDQIDWEVLIEAVPPKAARLLNKALYARLYRN
HHHCEEEEECCCCCCCCCCCCCHHCCCCCCCCCHHHHEECCCCHHHHHHHHHHHHHHHHH
HYDWLMHINSLFHAEKVVVNNRVDWPQRDRQIAQNLRRVCKNLSEDLKAPRLSRTFLIHQ
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LENRATVEKNLHRLPRSSHLLALYSESTDEYQARRLTRTYLDILKNKKHIKRWSLLRAAG
HHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
LSDERMTDIVSKLLEEILSE
CCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLGFPIPYSEELLYSTIARSGVHDGDVSPKQLLEKVFNNRRVIATVDLPSHVQAVANQYP
CCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCC
DSLGMDAKTLIYRHTLWPIYAPFLPPDRSDKVIKWMSGSSQGAAHLASGIVASRVKTKGR
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCC
LYVCVECLKEQRIKYGECFWERFWQIPIVNTCPKHGPLNKTTIVIDGEHRHTFTVIEDAE
HHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECHH
VLGKVNATNVDKLFSHQVSQLMQVISQGISPTQWTVFYEQFASNLGYLNGLRIDHSRIYD
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
SVIRFWGKGWLQKAGILPSYIETSWLKALFRKHRKSFSFAEHIVVITALSNGRFSICEAI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHCEEEEEEEECCCCHHHHHHH
DKASSIVIKSENNIPPKKRDLNTLNMLSPDQIDWEVLIEAVPPKAARLLNKALYARLYRN
HHHCEEEEECCCCCCCCCCCCCHHCCCCCCCCCHHHHEECCCCHHHHHHHHHHHHHHHHH
HYDWLMHINSLFHAEKVVVNNRVDWPQRDRQIAQNLRRVCKNLSEDLKAPRLSRTFLIHQ
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LENRATVEKNLHRLPRSSHLLALYSESTDEYQARRLTRTYLDILKNKKHIKRWSLLRAAG
HHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
LSDERMTDIVSKLLEEILSE
CCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576 [H]