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The map label for this gene is tnsE [H]

Identifier: 126176537

GI number: 126176537

Start: 5095833

End: 5097434

Strand: Reverse

Name: tnsE [H]

Synonym: Sbal_4356

Alternate gene names: 126176537

Gene position: 5097434-5095833 (Counterclockwise)

Preceding gene: 126176538

Following gene: 126176536

Centisome position: 99.42

GC content: 39.01

Gene sequence:

>1602_bases
GTGAGTAAGAGTGAAGTAAAATTCCAAAAATTAAACGATGATGCAAAGATATTAGGCATTGGAGGGTTGTTTCGAAGTGT
CGGTAATAAGTCTTGGGCCATTAACCTAGATTTTAATGGCAATACGTCACAAAGCCTGCAATTCAGTAATATTCCAGTAC
TTGCCCGTAAACGGGTGTTAAATGCCACAAAACCTTATGATCTAGCAGGGTTACCATTTTCGTTTACCATTTCAAATGCT
CAACAGTGGAGTGTTGCGAACGCGACTGATTGCCCTGCTTATCAGGCTCATAGGCATGGAAAAGACTCAAATCAACTTTG
TTTTGTCGTTCAATTAAGCGATGTTCTAGTTTATATTCCACAACTTGAAATGGCGCGGGTATTATTCTATCACGATCCAT
TTTTAGCTCGTTTAAGTTTGCAGCACAATGCGCTTATGGAAGATTTCTTTCTTAGTAAAAATGACAACGGTCAACCAGTT
GTGACAGTGCGTGAAGGCGCTGAATACCCTATTACATATTTTAACCAAGATGATAATAGACGATTCTTAACTTGGGTCCT
ACTAGATAGCGCTGCCAGAGCATCATTTGAAAGTATCAGTACTAACTTAGTAAAAAATCAGTATCAGAAAAATAAATATC
AATATTGGAATTTTCAATTCACTCCACCACCTCTTGATGGTGTAGATATTGGATTGCGAGGTTGGGAAGATCGGGAGTCA
AAAAGCTTCTTTGTATGGGAAATCATAAGTTTAAATGGATTGCCATCTGAAGTTACTGGTGAAATCGATTTTTTTCATCC
CGGCTATGAGCGACAAGTTGGCGGTAAACCTACAGTGGGTGATGGAAAGCAGGCTCAAGCACCGGATCAATATGAACTCG
ATGATGATGAATTGTCAGATACAGATAAAGCCACAATGGGATTAGCATCAGAGCGTGTAACCATTTCATTTAAGACACCA
TTCATAACCAATCGCGTAAGCAGAAAAATAAAGTCTGTTAGTAATGTATTTGGGGCGGATGAAACAGAGGTTTTAGACTC
AAGGCTTAGCACCAATGAAAAAGAAGACTCAGGTGAACTTCCTGGTGGAGCTTGGAATAATATTGACGACGAAACAGATG
ATGCGCATCTTTATCTTGGTAAGTTTCAGTCATTTATTAATATGGTTGATGTACTTGAATTGGCACATGGTTATGAGATA
TTAAGCAGAGAAATTATCAAGTTGCCGAGAGTTGGTGAGGGCAAAAAACACTGGCTATCCGATACACAAAACCCTAGGTG
TATGGCTGTTGTAAATCTAAGACATACTAATCAAAACATAACTCTATTTGAGATTGATACTTCAGATAACGCTGCAAAAT
TATCAACAATGATGATAAAGGGCGCTAATAAGGATGTATGGATTAGCCAAAATTTAAATCAAGTTATGGTGGAGATAATG
AAAAAGTCGTTAGGTTGGCCAACAGCTCTTTTTAAAACGGCATTCACGAAAGATGGATTTGCGGGTATCCCTCACCCTAA
ATCTAAAAATCCTGGCTCCTTACCCCCTGAAGAAATCGGCCCATGGGCACAGCGTTTTGCTAATTGGGCAAGTCGTTCAT
AA

Upstream 100 bases:

>100_bases
AATAAGAAACATATTAAACGATGGTCGCTATTGCGTGCAGCAGGCTTAAGTGATGAGCGGATGACAGATATTGTGAGTAA
GCTTTTAGAGGAGATTTTGA

Downstream 100 bases:

>100_bases
ATACGCAATCAAACTTGACAAAACTTGACGTAAGAATATACTTCGCACATGCAAAAAACACTTAATACTAAAAAAGCCAA
TTTGGCAATGGAACGAGCTG

Product: hypothetical protein

Products: NA

Alternate protein names: Protein D [H]

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MSKSEVKFQKLNDDAKILGIGGLFRSVGNKSWAINLDFNGNTSQSLQFSNIPVLARKRVLNATKPYDLAGLPFSFTISNA
QQWSVANATDCPAYQAHRHGKDSNQLCFVVQLSDVLVYIPQLEMARVLFYHDPFLARLSLQHNALMEDFFLSKNDNGQPV
VTVREGAEYPITYFNQDDNRRFLTWVLLDSAARASFESISTNLVKNQYQKNKYQYWNFQFTPPPLDGVDIGLRGWEDRES
KSFFVWEIISLNGLPSEVTGEIDFFHPGYERQVGGKPTVGDGKQAQAPDQYELDDDELSDTDKATMGLASERVTISFKTP
FITNRVSRKIKSVSNVFGADETEVLDSRLSTNEKEDSGELPGGAWNNIDDETDDAHLYLGKFQSFINMVDVLELAHGYEI
LSREIIKLPRVGEGKKHWLSDTQNPRCMAVVNLRHTNQNITLFEIDTSDNAAKLSTMMIKGANKDVWISQNLNQVMVEIM
KKSLGWPTALFKTAFTKDGFAGIPHPKSKNPGSLPPEEIGPWAQRFANWASRS

Sequences:

>Translated_533_residues
MSKSEVKFQKLNDDAKILGIGGLFRSVGNKSWAINLDFNGNTSQSLQFSNIPVLARKRVLNATKPYDLAGLPFSFTISNA
QQWSVANATDCPAYQAHRHGKDSNQLCFVVQLSDVLVYIPQLEMARVLFYHDPFLARLSLQHNALMEDFFLSKNDNGQPV
VTVREGAEYPITYFNQDDNRRFLTWVLLDSAARASFESISTNLVKNQYQKNKYQYWNFQFTPPPLDGVDIGLRGWEDRES
KSFFVWEIISLNGLPSEVTGEIDFFHPGYERQVGGKPTVGDGKQAQAPDQYELDDDELSDTDKATMGLASERVTISFKTP
FITNRVSRKIKSVSNVFGADETEVLDSRLSTNEKEDSGELPGGAWNNIDDETDDAHLYLGKFQSFINMVDVLELAHGYEI
LSREIIKLPRVGEGKKHWLSDTQNPRCMAVVNLRHTNQNITLFEIDTSDNAAKLSTMMIKGANKDVWISQNLNQVMVEIM
KKSLGWPTALFKTAFTKDGFAGIPHPKSKNPGSLPPEEIGPWAQRFANWASRS
>Mature_532_residues
SKSEVKFQKLNDDAKILGIGGLFRSVGNKSWAINLDFNGNTSQSLQFSNIPVLARKRVLNATKPYDLAGLPFSFTISNAQ
QWSVANATDCPAYQAHRHGKDSNQLCFVVQLSDVLVYIPQLEMARVLFYHDPFLARLSLQHNALMEDFFLSKNDNGQPVV
TVREGAEYPITYFNQDDNRRFLTWVLLDSAARASFESISTNLVKNQYQKNKYQYWNFQFTPPPLDGVDIGLRGWEDRESK
SFFVWEIISLNGLPSEVTGEIDFFHPGYERQVGGKPTVGDGKQAQAPDQYELDDDELSDTDKATMGLASERVTISFKTPF
ITNRVSRKIKSVSNVFGADETEVLDSRLSTNEKEDSGELPGGAWNNIDDETDDAHLYLGKFQSFINMVDVLELAHGYEIL
SREIIKLPRVGEGKKHWLSDTQNPRCMAVVNLRHTNQNITLFEIDTSDNAAKLSTMMIKGANKDVWISQNLNQVMVEIMK
KSLGWPTALFKTAFTKDGFAGIPHPKSKNPGSLPPEEIGPWAQRFANWASRS

Specific function: TnsABC + tnsD promote high-frequency insertion of Tn7 into a specific target site known as att-Tn7 whereas tnsABC + tnsE promote low-frequency insertion into many different sites [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016421 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 60031; Mature: 59900

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSEVKFQKLNDDAKILGIGGLFRSVGNKSWAINLDFNGNTSQSLQFSNIPVLARKRVL
CCCCCCCCEECCCCCEEEEEHHHHHHCCCCEEEEEEEECCCCCCCEEECCCCHHHHHHHH
NATKPYDLAGLPFSFTISNAQQWSVANATDCPAYQAHRHGKDSNQLCFVVQLSDVLVYIP
CCCCCCCCCCCCEEEEECCCCEECCCCCCCCCCHHHHHCCCCCCCEEEEEEECCEEEECC
QLEMARVLFYHDPFLARLSLQHNALMEDFFLSKNDNGQPVVTVREGAEYPITYFNQDDNR
CHHHHHHHHCCCCHHEEEEHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEECCCCCC
RFLTWVLLDSAARASFESISTNLVKNQYQKNKYQYWNFQFTPPPLDGVDIGLRGWEDRES
EEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEECCCCCCCCCC
KSFFVWEIISLNGLPSEVTGEIDFFHPGYERQVGGKPTVGDGKQAQAPDQYELDDDELSD
CCEEEEEEEECCCCCHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TDKATMGLASERVTISFKTPFITNRVSRKIKSVSNVFGADETEVLDSRLSTNEKEDSGEL
CCHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCC
PGGAWNNIDDETDDAHLYLGKFQSFINMVDVLELAHGYEILSREIIKLPRVGEGKKHWLS
CCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCC
DTQNPRCMAVVNLRHTNQNITLFEIDTSDNAAKLSTMMIKGANKDVWISQNLNQVMVEIM
CCCCCEEEEEEEEEECCCCEEEEEEECCCCHHEEEEEEEECCCCCEEEECCHHHHHHHHH
KKSLGWPTALFKTAFTKDGFAGIPHPKSKNPGSLPPEEIGPWAQRFANWASRS
HHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SKSEVKFQKLNDDAKILGIGGLFRSVGNKSWAINLDFNGNTSQSLQFSNIPVLARKRVL
CCCCCCCEECCCCCEEEEEHHHHHHCCCCEEEEEEEECCCCCCCEEECCCCHHHHHHHH
NATKPYDLAGLPFSFTISNAQQWSVANATDCPAYQAHRHGKDSNQLCFVVQLSDVLVYIP
CCCCCCCCCCCCEEEEECCCCEECCCCCCCCCCHHHHHCCCCCCCEEEEEEECCEEEECC
QLEMARVLFYHDPFLARLSLQHNALMEDFFLSKNDNGQPVVTVREGAEYPITYFNQDDNR
CHHHHHHHHCCCCHHEEEEHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEECCCCCC
RFLTWVLLDSAARASFESISTNLVKNQYQKNKYQYWNFQFTPPPLDGVDIGLRGWEDRES
EEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEECCCCCCCCCC
KSFFVWEIISLNGLPSEVTGEIDFFHPGYERQVGGKPTVGDGKQAQAPDQYELDDDELSD
CCEEEEEEEECCCCCHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TDKATMGLASERVTISFKTPFITNRVSRKIKSVSNVFGADETEVLDSRLSTNEKEDSGEL
CCHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCC
PGGAWNNIDDETDDAHLYLGKFQSFINMVDVLELAHGYEILSREIIKLPRVGEGKKHWLS
CCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCC
DTQNPRCMAVVNLRHTNQNITLFEIDTSDNAAKLSTMMIKGANKDVWISQNLNQVMVEIM
CCCCCEEEEEEEEEECCCCEEEEEEECCCCHHEEEEEEEECCCCCEEEECCHHHHHHHHH
KKSLGWPTALFKTAFTKDGFAGIPHPKSKNPGSLPPEEIGPWAQRFANWASRS
HHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2156235; 3022239 [H]