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The map label for this gene is hsdS [C]

Identifier: 126175911

GI number: 126175911

Start: 4345846

End: 4347129

Strand: Reverse

Name: hsdS [C]

Synonym: Sbal_3717

Alternate gene names: 126175911

Gene position: 4347129-4345846 (Counterclockwise)

Preceding gene: 126175912

Following gene: 126175910

Centisome position: 84.78

GC content: 37.15

Gene sequence:

>1284_bases
ATGAATAAGCTAGAGGAAAAAGGTTTAGTGCCAAAGTTGAGGTTTTCTGAGTTTAAGGATGCACCTGAATGGAGCCCCAC
CACTTTTGGCGCAACAGCTACCTTTATTAACGGTAAGGCATACAAACAGGAAGAACTACTTGAAAATGGAAAATATCGAG
TACTAAGAGTCGGGAACTTTTTTACAAATAAAGAATGGTATTTTTCTGACTTAGAACTAGATGAAAATAAATATTGTGAT
AATGGTGATCTTCTCTATGCTTGGTCTGCTTCTTTTGGACCAAGGATATGGTTAGGTGAAAAAGTAATTTATCACTACCA
TATATGGAAAGTTTTAGAAAAAAAACATATAGATAAAAATTTCTTATTTATATTACTTGACTATGAAACTGAGCGAATGA
AAGCTGCTACTGCAAATGGCCTAGGGTTGATGCATATAACCAAGAGCTCTATTGAAAACTGGAAATGCTGCATTCCGAGT
AGTATAGAAGAACAAAAAAAAATCGCTAATTCCCTTTCATCTCTTGATGAACTGATAAGCGCACACACCCAAAAATTTGA
CACCTTAAAAGCCTATAAGAAAGGCCTAATGCAACAACTCTTCCCCGCCGAAGGTGAAACCGTCCCCAAGCTCAGATTTC
CTGAGTTTCAGGGGGAATGGCGAAAAACTCAACTTAAAAAACTCGGAGAACTTGTATCAGGTTTAACGTATAGCCCTGCT
GATGTTAGAGATTCAGGCCTTTTGGTTTTAAGGTCTTCAAATGTTAAAAATGGAATAATTAGCTTGAAGGACAATGTATA
CGTTACTCCGAATGTAAAGGGAGCTAACTTATCCAAAGCTAATGATATTTTAATCTGTGTGAGGAATGGTTCAAAGGCAC
TAATAGGTAAAAACGCATTAATTCCAGAAGGAATGCCTGTATGTACTCATGGTGCATTCATGACAGTATTTAGATCTCCT
TCTGCTAAATTTGTATTTCAACTGTTTCAAACTAATGCTTATCAGAAACAAGTTGATGCTGACTTAGGGGCAACAATTAA
TTCAATTAATGGCCGCCATTTTATTAAGTATGAGTTTTACGTACCAGAATCGTTTGAACAACAAAAAATTGCCGACTGTT
TATCATCTATAGATGAATTAATCACTGCGCAAAGCCACAAAATAGACGCCCTTAAAGTACACAAGCAAGGCTTAATGCAG
CAACTGTTTCCGACGGTTGGTGTTGATTGCATTGATGCAGAAGATAGAGCAACGCAAAATGCCGAAGTAGAGGTGAGCGC
ATGA

Upstream 100 bases:

>100_bases
TTGCAAGCTAAACTGCACCAGTTATTACAAGACCTGCCTGCGCAAGACGAATTAGCGAGTCAGCACATGACTGAACAAAT
GCCAAGGCAAGGAGAATACA

Downstream 100 bases:

>100_bases
GCGAATTAACCTTTGCTTCACTCGATGGCGTGGCGGCACATTTACGCCATGTGTTAGAAAACGGCATACCACAACCCCAG
AACAAGTTAAAACATATCAA

Product: restriction modification system DNA specificity subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNKLEEKGLVPKLRFSEFKDAPEWSPTTFGATATFINGKAYKQEELLENGKYRVLRVGNFFTNKEWYFSDLELDENKYCD
NGDLLYAWSASFGPRIWLGEKVIYHYHIWKVLEKKHIDKNFLFILLDYETERMKAATANGLGLMHITKSSIENWKCCIPS
SIEEQKKIANSLSSLDELISAHTQKFDTLKAYKKGLMQQLFPAEGETVPKLRFPEFQGEWRKTQLKKLGELVSGLTYSPA
DVRDSGLLVLRSSNVKNGIISLKDNVYVTPNVKGANLSKANDILICVRNGSKALIGKNALIPEGMPVCTHGAFMTVFRSP
SAKFVFQLFQTNAYQKQVDADLGATINSINGRHFIKYEFYVPESFEQQKIADCLSSIDELITAQSHKIDALKVHKQGLMQ
QLFPTVGVDCIDAEDRATQNAEVEVSA

Sequences:

>Translated_427_residues
MNKLEEKGLVPKLRFSEFKDAPEWSPTTFGATATFINGKAYKQEELLENGKYRVLRVGNFFTNKEWYFSDLELDENKYCD
NGDLLYAWSASFGPRIWLGEKVIYHYHIWKVLEKKHIDKNFLFILLDYETERMKAATANGLGLMHITKSSIENWKCCIPS
SIEEQKKIANSLSSLDELISAHTQKFDTLKAYKKGLMQQLFPAEGETVPKLRFPEFQGEWRKTQLKKLGELVSGLTYSPA
DVRDSGLLVLRSSNVKNGIISLKDNVYVTPNVKGANLSKANDILICVRNGSKALIGKNALIPEGMPVCTHGAFMTVFRSP
SAKFVFQLFQTNAYQKQVDADLGATINSINGRHFIKYEFYVPESFEQQKIADCLSSIDELITAQSHKIDALKVHKQGLMQ
QLFPTVGVDCIDAEDRATQNAEVEVSA
>Mature_427_residues
MNKLEEKGLVPKLRFSEFKDAPEWSPTTFGATATFINGKAYKQEELLENGKYRVLRVGNFFTNKEWYFSDLELDENKYCD
NGDLLYAWSASFGPRIWLGEKVIYHYHIWKVLEKKHIDKNFLFILLDYETERMKAATANGLGLMHITKSSIENWKCCIPS
SIEEQKKIANSLSSLDELISAHTQKFDTLKAYKKGLMQQLFPAEGETVPKLRFPEFQGEWRKTQLKKLGELVSGLTYSPA
DVRDSGLLVLRSSNVKNGIISLKDNVYVTPNVKGANLSKANDILICVRNGSKALIGKNALIPEGMPVCTHGAFMTVFRSP
SAKFVFQLFQTNAYQKQVDADLGATINSINGRHFIKYEFYVPESFEQQKIADCLSSIDELITAQSHKIDALKVHKQGLMQ
QLFPTVGVDCIDAEDRATQNAEVEVSA

Specific function: The M And S Subunits Together Form A Methyltransferase (Mtase) That Methylates Two Adenine Residues In Complementary Strands Of A Bipartite DNA Recognition Sequence. In The Presence Of The R Subunit The Complex Can Also Act As An Endonuclease, Binding To

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790807, Length=168, Percent_Identity=27.9761904761905, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 48282; Mature: 48282

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLEEKGLVPKLRFSEFKDAPEWSPTTFGATATFINGKAYKQEELLENGKYRVLRVGNF
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCEEEEEECCE
FTNKEWYFSDLELDENKYCDNGDLLYAWSASFGPRIWLGEKVIYHYHIWKVLEKKHIDKN
ECCCCEEEEECEECCCCCCCCCCEEEEECCCCCCEEEECCEEEEHHHHHHHHHHHCCCCC
FLFILLDYETERMKAATANGLGLMHITKSSIENWKCCIPSSIEEQKKIANSLSSLDELIS
EEEEEEECCHHHHHHHHCCCEEEEEEEHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHH
AHTQKFDTLKAYKKGLMQQLFPAEGETVPKLRFPEFQGEWRKTQLKKLGELVSGLTYSPA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCC
DVRDSGLLVLRSSNVKNGIISLKDNVYVTPNVKGANLSKANDILICVRNGSKALIGKNAL
CCCCCCEEEEECCCCCCCEEEECCCEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCC
IPEGMPVCTHGAFMTVFRSPSAKFVFQLFQTNAYQKQVDADLGATINSINGRHFIKYEFY
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHCCEEECCCCEEEEEEEEE
VPESFEQQKIADCLSSIDELITAQSHKIDALKVHKQGLMQQLFPTVGVDCIDAEDRATQN
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCC
AEVEVSA
CEEEEEC
>Mature Secondary Structure
MNKLEEKGLVPKLRFSEFKDAPEWSPTTFGATATFINGKAYKQEELLENGKYRVLRVGNF
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCEEEEEECCE
FTNKEWYFSDLELDENKYCDNGDLLYAWSASFGPRIWLGEKVIYHYHIWKVLEKKHIDKN
ECCCCEEEEECEECCCCCCCCCCEEEEECCCCCCEEEECCEEEEHHHHHHHHHHHCCCCC
FLFILLDYETERMKAATANGLGLMHITKSSIENWKCCIPSSIEEQKKIANSLSSLDELIS
EEEEEEECCHHHHHHHHCCCEEEEEEEHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHH
AHTQKFDTLKAYKKGLMQQLFPAEGETVPKLRFPEFQGEWRKTQLKKLGELVSGLTYSPA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCC
DVRDSGLLVLRSSNVKNGIISLKDNVYVTPNVKGANLSKANDILICVRNGSKALIGKNAL
CCCCCCEEEEECCCCCCCEEEECCCEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCC
IPEGMPVCTHGAFMTVFRSPSAKFVFQLFQTNAYQKQVDADLGATINSINGRHFIKYEFY
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHCCEEECCCCEEEEEEEEE
VPESFEQQKIADCLSSIDELITAQSHKIDALKVHKQGLMQQLFPTVGVDCIDAEDRATQN
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCC
AEVEVSA
CEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]