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The map label for this gene is garR [C]

Identifier: 126173953

GI number: 126173953

Start: 2003571

End: 2004446

Strand: Direct

Name: garR [C]

Synonym: Sbal_1724

Alternate gene names: 126173953

Gene position: 2003571-2004446 (Clockwise)

Preceding gene: 126173952

Following gene: 126173955

Centisome position: 39.08

GC content: 51.14

Gene sequence:

>876_bases
ATGGCAAAAGTCGCATTTATTGGTTTAGGCGTAATGGGATATCCCATGGCGAGGCATTTACTGAACAAAGGCCATGAGGT
CACTGTTTATAATCGCACTTTTGCTAAGGCGCAAACTTGGGTCGACACCTATGGCGGACGCTGCTGCCCAACGCCAAAGG
AAGCGGCTCAGGGTCAAGATATTGTGTTTACCTGTGTGGGCAATGATAACGATCTGCGTGAAGTGGTATTAGGGAGTGAT
GGCGTTATTCATGGCATGGAAAAGGGCGCAGTGCTGGTTGACCATACTACGGCTTCGGCCGATGTTGCCCGTGAGCTTCA
TCAAGTGTTAGCGGAAAAGGGCGTTGATTTCCTCGATGCGCCTGTGTCGGGCGGTCAAGCGGGCGCCGAAAATGGCGCCT
TGACTGTGATGGTTGGCGGCGATGAAGCTGTGTTTGTCCGTGTTAAACCTGTGATTGAAGCCTTTGCACGCTGCGCCGAG
CGTTTAGGTGAGGTTGGTGCAGGTCAGTTAACTAAGATGGTGAACCAAATCTGTATCGCGGGTGTGGTGCAAGGTTTAGC
TGAAGCATTACAGTTTGCGCGCAAGGCAGGACTCGATGGCGAGAAAGTCATTGAAGTGATTAGCAAAGGCGCGGCGCAAA
GCTGGCAAATGGAAAATCGCTACAAGACGATGTGGGGCCAAAGCTATGATTTTGGTTTTGCCGTCGATTGGATGCGTAAA
GATTTAGGCATCGCCTTAGAAGAAGCTCGCCGCAATGGTTCACACTTGCCATTAACCGCGTTAGTCGATCAATTCTATTC
AGAAGTACAGGGCATGGGCGGTAACCGTTGGGATACGTCTAGCCTGTTGGCGCGCCTAGAGAAAGCGGCGAAATAA

Upstream 100 bases:

>100_bases
TTGTTGAGGTTTTAGCGGTAACATATTGGGCAGATGAAACACAACTCGATTCAGCAATTTCTACTCGATTCACCTTGTTT
ACCTATTTGAGGATCACTTC

Downstream 100 bases:

>100_bases
GTTTCATCGTTATAAACAATTAGGTCTCGGAGTGATAAACACTCTGGTTTCGAAGTGATAAAAACTTGGCTTTCGGAGTG
ATAAACACTCAGATTTCGCA

Product: 6-phosphogluconate dehydrogenase NAD-binding

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MAKVAFIGLGVMGYPMARHLLNKGHEVTVYNRTFAKAQTWVDTYGGRCCPTPKEAAQGQDIVFTCVGNDNDLREVVLGSD
GVIHGMEKGAVLVDHTTASADVARELHQVLAEKGVDFLDAPVSGGQAGAENGALTVMVGGDEAVFVRVKPVIEAFARCAE
RLGEVGAGQLTKMVNQICIAGVVQGLAEALQFARKAGLDGEKVIEVISKGAAQSWQMENRYKTMWGQSYDFGFAVDWMRK
DLGIALEEARRNGSHLPLTALVDQFYSEVQGMGGNRWDTSSLLARLEKAAK

Sequences:

>Translated_291_residues
MAKVAFIGLGVMGYPMARHLLNKGHEVTVYNRTFAKAQTWVDTYGGRCCPTPKEAAQGQDIVFTCVGNDNDLREVVLGSD
GVIHGMEKGAVLVDHTTASADVARELHQVLAEKGVDFLDAPVSGGQAGAENGALTVMVGGDEAVFVRVKPVIEAFARCAE
RLGEVGAGQLTKMVNQICIAGVVQGLAEALQFARKAGLDGEKVIEVISKGAAQSWQMENRYKTMWGQSYDFGFAVDWMRK
DLGIALEEARRNGSHLPLTALVDQFYSEVQGMGGNRWDTSSLLARLEKAAK
>Mature_290_residues
AKVAFIGLGVMGYPMARHLLNKGHEVTVYNRTFAKAQTWVDTYGGRCCPTPKEAAQGQDIVFTCVGNDNDLREVVLGSDG
VIHGMEKGAVLVDHTTASADVARELHQVLAEKGVDFLDAPVSGGQAGAENGALTVMVGGDEAVFVRVKPVIEAFARCAER
LGEVGAGQLTKMVNQICIAGVVQGLAEALQFARKAGLDGEKVIEVISKGAAQSWQMENRYKTMWGQSYDFGFAVDWMRKD
LGIALEEARRNGSHLPLTALVDQFYSEVQGMGGNRWDTSSLLARLEKAAK

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=280, Percent_Identity=28.5714285714286, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI23308751, Length=293, Percent_Identity=30.3754266211604, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI145693186, Length=289, Percent_Identity=37.3702422145329, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1786719, Length=289, Percent_Identity=35.9861591695502, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1790315, Length=295, Percent_Identity=29.4915254237288, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789092, Length=295, Percent_Identity=32.5423728813559, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788341, Length=302, Percent_Identity=25.1655629139073, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17557316, Length=275, Percent_Identity=27.6363636363636, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24655230, Length=298, Percent_Identity=31.5436241610738, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI19922568, Length=298, Percent_Identity=31.5436241610738, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI28574115, Length=281, Percent_Identity=24.1992882562278, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31321; Mature: 31190

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVAFIGLGVMGYPMARHLLNKGHEVTVYNRTFAKAQTWVDTYGGRCCPTPKEAAQGQD
CCEEEEECCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCHHHCCCCC
IVFTCVGNDNDLREVVLGSDGVIHGMEKGAVLVDHTTASADVARELHQVLAEKGVDFLDA
EEEEEECCCCCHHHHHCCCCCCEECCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEECC
PVSGGQAGAENGALTVMVGGDEAVFVRVKPVIEAFARCAERLGEVGAGQLTKMVNQICIA
CCCCCCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GVVQGLAEALQFARKAGLDGEKVIEVISKGAAQSWQMENRYKTMWGQSYDFGFAVDWMRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
DLGIALEEARRNGSHLPLTALVDQFYSEVQGMGGNRWDTSSLLARLEKAAK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AKVAFIGLGVMGYPMARHLLNKGHEVTVYNRTFAKAQTWVDTYGGRCCPTPKEAAQGQD
CEEEEECCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCHHHCCCCC
IVFTCVGNDNDLREVVLGSDGVIHGMEKGAVLVDHTTASADVARELHQVLAEKGVDFLDA
EEEEEECCCCCHHHHHCCCCCCEECCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEECC
PVSGGQAGAENGALTVMVGGDEAVFVRVKPVIEAFARCAERLGEVGAGQLTKMVNQICIA
CCCCCCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GVVQGLAEALQFARKAGLDGEKVIEVISKGAAQSWQMENRYKTMWGQSYDFGFAVDWMRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
DLGIALEEARRNGSHLPLTALVDQFYSEVQGMGGNRWDTSSLLARLEKAAK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA