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The map label for this gene is oprF [H]

Identifier: 126173289

GI number: 126173289

Start: 1203003

End: 1204094

Strand: Reverse

Name: oprF [H]

Synonym: Sbal_1045

Alternate gene names: 126173289

Gene position: 1204094-1203003 (Counterclockwise)

Preceding gene: 126173290

Following gene: 126173288

Centisome position: 23.48

GC content: 42.67

Gene sequence:

>1092_bases
ATGAATCATAAATTAAAATTTGTACTGCTTGCATCGCTATTACCTTTCACTGTATCAGCGGCTCAAGAACTGACTCCTTG
GTATGTTGGCGCAGGTTTAGGCGTTAACAACTATGAACATATCGCGACCCAGAATGGTCAAGATGACCCATATGCATGGG
ATGTATTCGCAGGCTATATGTTCAATGATTACTTCGGCGCTGAAATCGGCTTCCGTGATTTAGGTAATGCAGATTGGACT
AATGGGGCCACCAGTAATGATGCAGGCGTTAAAGGTTCAACTTTAGGTTTAGTCGGTATTTGGCCACTAGCTAATCGTTG
GAGCTTATCAGCTGAAGCGGGTGCTATGTATTATTCACTTGAGAACAGCCAAAATAATGGCATTACATCAAGCAAATACA
GTACAAACGACTTTGCTCCATACTTAGGTGCAGGTGTTGGTTACAACTTCACTGATAACCTGAAGTTACAAGCTAAATAT
CGTCGTTATGAAAACCTAAATGACGATTACAACATCGTTGAAGCTGATAGCAACTACTGGGGTCTTGAACTGAGCTACCG
TTTCGGTTCTCCAGCAGCTGCAGCACCTGTTGCAGCTGCGGTTATCGCAGCAGCACCTGTTGACTCAGACAACGATGGTG
TTTATGACGATAAAGATCAATGTCCAGCTACACCAGTAACGCATAAAGTTGATTCTGTTGGCTGTACAATTTACGAAAAC
GTAAAAAATAAAGAAGACGTGGGTTCAATCCAGTTTGCTAACGATTCTGCTGTAGTTAAAAAAGTTTTCTACAAAGATAT
CGAAAGACTCGCTAACTACCTGAACAAAAACCCAGAGTTCACAGTTGAAATCGCTGGCCACGCCTCAAACGTAGGTAAAC
CAGAGTACAACATGGTGCTGTCTGACAAACGTGCTGACGCAGTTGCTCAAATTCTTGTTGAGAAATACGGTATCAGCAAA
AACCGCGTAACTTCTAACGGTTACGGTGTTACTCAGCCATTGGTTGCTGGTGACTCGAAAGAAGCTTACGCTGCTAACCG
TCGTATCGAAGCCATTGTAACGACTACAACTAAACAACCTGTTCTTAAGTAA

Upstream 100 bases:

>100_bases
CAATACCAAAGGATCCCCACACTAAGTGCTAGCAATGTTTTGCACATATACGGCAAGACATATTGGTGGAGAATCATAAA
TAATGGAATTGGAGAACATG

Downstream 100 bases:

>100_bases
GATTACTTACTGACGTAAAAAAGTCGCTGCTTGCAGCGACTTTTTGTTTTTATGGGGTATACACTAGGACAAGCGATGCC
CTCTACCGCAGCCAAGATGA

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MNHKLKFVLLASLLPFTVSAAQELTPWYVGAGLGVNNYEHIATQNGQDDPYAWDVFAGYMFNDYFGAEIGFRDLGNADWT
NGATSNDAGVKGSTLGLVGIWPLANRWSLSAEAGAMYYSLENSQNNGITSSKYSTNDFAPYLGAGVGYNFTDNLKLQAKY
RRYENLNDDYNIVEADSNYWGLELSYRFGSPAAAAPVAAAVIAAAPVDSDNDGVYDDKDQCPATPVTHKVDSVGCTIYEN
VKNKEDVGSIQFANDSAVVKKVFYKDIERLANYLNKNPEFTVEIAGHASNVGKPEYNMVLSDKRADAVAQILVEKYGISK
NRVTSNGYGVTQPLVAGDSKEAYAANRRIEAIVTTTTKQPVLK

Sequences:

>Translated_363_residues
MNHKLKFVLLASLLPFTVSAAQELTPWYVGAGLGVNNYEHIATQNGQDDPYAWDVFAGYMFNDYFGAEIGFRDLGNADWT
NGATSNDAGVKGSTLGLVGIWPLANRWSLSAEAGAMYYSLENSQNNGITSSKYSTNDFAPYLGAGVGYNFTDNLKLQAKY
RRYENLNDDYNIVEADSNYWGLELSYRFGSPAAAAPVAAAVIAAAPVDSDNDGVYDDKDQCPATPVTHKVDSVGCTIYEN
VKNKEDVGSIQFANDSAVVKKVFYKDIERLANYLNKNPEFTVEIAGHASNVGKPEYNMVLSDKRADAVAQILVEKYGISK
NRVTSNGYGVTQPLVAGDSKEAYAANRRIEAIVTTTTKQPVLK
>Mature_363_residues
MNHKLKFVLLASLLPFTVSAAQELTPWYVGAGLGVNNYEHIATQNGQDDPYAWDVFAGYMFNDYFGAEIGFRDLGNADWT
NGATSNDAGVKGSTLGLVGIWPLANRWSLSAEAGAMYYSLENSQNNGITSSKYSTNDFAPYLGAGVGYNFTDNLKLQAKY
RRYENLNDDYNIVEADSNYWGLELSYRFGSPAAAAPVAAAVIAAAPVDSDNDGVYDDKDQCPATPVTHKVDSVGCTIYEN
VKNKEDVGSIQFANDSAVVKKVFYKDIERLANYLNKNPEFTVEIAGHASNVGKPEYNMVLSDKRADAVAQILVEKYGISK
NRVTSNGYGVTQPLVAGDSKEAYAANRRIEAIVTTTTKQPVLK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1786962, Length=99, Percent_Identity=36.3636363636364, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787191, Length=359, Percent_Identity=23.3983286908078, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 39559; Mature: 39559

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHKLKFVLLASLLPFTVSAAQELTPWYVGAGLGVNNYEHIATQNGQDDPYAWDVFAGYM
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHCCCCCCCCEEEEHHHHHH
FNDYFGAEIGFRDLGNADWTNGATSNDAGVKGSTLGLVGIWPLANRWSLSAEAGAMYYSL
HHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEECCCCCCEEEEEE
ENSQNNGITSSKYSTNDFAPYLGAGVGYNFTDNLKLQAKYRRYENLNDDYNIVEADSNYW
ECCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEEHHHHHCCCCCCCEEEECCCCEE
GLELSYRFGSPAAAAPVAAAVIAAAPVDSDNDGVYDDKDQCPATPVTHKVDSVGCTIYEN
EEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
VKNKEDVGSIQFANDSAVVKKVFYKDIERLANYLNKNPEFTVEIAGHASNVGKPEYNMVL
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEE
SDKRADAVAQILVEKYGISKNRVTSNGYGVTQPLVAGDSKEAYAANRRIEAIVTTTTKQP
CCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCEECCCCCHHHHHCCEEEEEEEECCCCC
VLK
CCC
>Mature Secondary Structure
MNHKLKFVLLASLLPFTVSAAQELTPWYVGAGLGVNNYEHIATQNGQDDPYAWDVFAGYM
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHCCCCCCCCEEEEHHHHHH
FNDYFGAEIGFRDLGNADWTNGATSNDAGVKGSTLGLVGIWPLANRWSLSAEAGAMYYSL
HHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEECCCCCCEEEEEE
ENSQNNGITSSKYSTNDFAPYLGAGVGYNFTDNLKLQAKYRRYENLNDDYNIVEADSNYW
ECCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEEHHHHHCCCCCCCEEEECCCCEE
GLELSYRFGSPAAAAPVAAAVIAAAPVDSDNDGVYDDKDQCPATPVTHKVDSVGCTIYEN
EEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
VKNKEDVGSIQFANDSAVVKKVFYKDIERLANYLNKNPEFTVEIAGHASNVGKPEYNMVL
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEE
SDKRADAVAQILVEKYGISKNRVTSNGYGVTQPLVAGDSKEAYAANRRIEAIVTTTTKQP
CCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCEECCCCCHHHHHCCEEEEEEEECCCCC
VLK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]