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The map label for this gene is galP [H]

Identifier: 125625004

GI number: 125625004

Start: 2199576

End: 2200964

Strand: Reverse

Name: galP [H]

Synonym: llmg_2237

Alternate gene names: 125625004

Gene position: 2200964-2199576 (Counterclockwise)

Preceding gene: 125625005

Following gene: 125625003

Centisome position: 87.01

GC content: 38.16

Gene sequence:

>1389_bases
ATGAAAGAGGGAAAAATGAAACAACGTTTATCGTACGCTTTTGGTGCACTAGGACATGATGTTTACTACTATTCAATTAG
TACATTCTTCATTGCCTTTGTAACGGCACAAATGTTTGCCGGTTCACCTCATGAGGATGCAATGATTGCTTTAGTGACAG
GCTTGGTTGTTATTATTCGTTTGGTTGAAATTATTTTTGACCCAATTATTGGTTCGATTATTGATAATACACACACAAGA
TGGGGTAAATTTAAACCTTGGTTGGTCGTTGGTGGGATTATGTCATCTTTGATGATTATGCTCATGTTTTCAGACTTCTT
CGGATTAGCCAAAAGTGGAAATCGGACATTGTTTGCCATCGTATTCATTATTGCATTTATTATTCTTGATGCTTTCTACT
CATTCAAGGATATTGCTTTCTGGTCAATGATTCCAGCTTTATCAGAAAAAAATTCTGAACGGGAAACACTTGGAACATTC
GCTCGTTTTGGTTCAGCAATTGGGGCACAAGGAGCAACAATTTTAGCAATACCAATTACAATTTTCTTCACTAAAGGTGG
ACATGCACAAGGTGCAAGAGGTTTCTTCGCTTTCGGTGTCATTGCCGCGTTGGTTCAAGGGATTTCTGCCCTAGTGACTG
CATGGGGAACTAAAGAACAAAAATCAGTGATTCGTCAAGAAGGAACGAAAACAAATACCTCAGATGTTTTCAAAGCCCTT
CTCAAAAATGACCAATTGATGTGGTTGTCACTCTCATATATTTTATTTGCCATTGCTTATGTAGCAACAACAGCAACCTT
AATCCTGAACTTTACTTTCGTTATTGGTAATGCTAGTCTTTATTCAATCACAGGGATTGTCGGATTTATTGGTTCAATCA
TTTTAGTGCCAATGTTCCCAACCTTGGCTAAAAAATTTGGACGCCGTAAAGTTTTGACAGGGGCCATTATTTCAATGCTT
GTCGGTTATGTCCTCTTTGTTTTAGGAAATTCAGTACCAATGACAGTGGGCGGATTAATCTTTTTGACAGCACCTTATCA
ACTCGTTTTCTTGTCAGTTTTGATGACCATCACCGACTCTGTAGAATACGGTCAATGGAAAAATGGAGTTCGTAATGAAG
CAGTAACGCTTGCGATGCGTCCATTACTTGATAAGATTGCGGGTGCCTTTTCAAATGGTATTTATGGATTTGTAGCGATT
GCGGCAGGAATGACCGGTTCAAAATATATCTCTGGGCACACTTATGGTGTTGCAACTTTCAAACTTTATTCCTTTGTAGT
CCCAGCGGTCTTAATGATTATCGCTCTAGCTGTTTATTTACTCAAAGTTAAATTAACTGAAAAACGTCATGAAGAAATCG
TTGCAGAACTTGAAGAACGTTTGAAATAA

Upstream 100 bases:

>100_bases
ATAGAAATGAGTGATGTAAATCACTTCGTACTCAAAAACAAGGCAGTATCTCTGCAGTTCTACTCAAAAATGAGTAGATC
TAGTATAAAGGAGGATTCCC

Downstream 100 bases:

>100_bases
GTAAAACAATTTACTGACAGTACATAGATTTATTAGTAAACTTTAAAATACAATAATTTTTGTACTGATCAAGCTGTCAG
TTGGGCTATAAAATACTGAC

Product: galactose permease

Products: Li (I) [Cytoplasm]; melibiose [Cytoplasm]; Na (I) [Cytoplasm]; melibiose [Cytoplasm]; Proton [Cytoplasm]; melibiose [Cytoplasm] [C]

Alternate protein names: Lactose transport protein; Lactose-proton symporter; Putative phosphotransferase enzyme IIA component; Putative PTS system EIIA component [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKEGKMKQRLSYAFGALGHDVYYYSISTFFIAFVTAQMFAGSPHEDAMIALVTGLVVIIRLVEIIFDPIIGSIIDNTHTR
WGKFKPWLVVGGIMSSLMIMLMFSDFFGLAKSGNRTLFAIVFIIAFIILDAFYSFKDIAFWSMIPALSEKNSERETLGTF
ARFGSAIGAQGATILAIPITIFFTKGGHAQGARGFFAFGVIAALVQGISALVTAWGTKEQKSVIRQEGTKTNTSDVFKAL
LKNDQLMWLSLSYILFAIAYVATTATLILNFTFVIGNASLYSITGIVGFIGSIILVPMFPTLAKKFGRRKVLTGAIISML
VGYVLFVLGNSVPMTVGGLIFLTAPYQLVFLSVLMTITDSVEYGQWKNGVRNEAVTLAMRPLLDKIAGAFSNGIYGFVAI
AAGMTGSKYISGHTYGVATFKLYSFVVPAVLMIIALAVYLLKVKLTEKRHEEIVAELEERLK

Sequences:

>Translated_462_residues
MKEGKMKQRLSYAFGALGHDVYYYSISTFFIAFVTAQMFAGSPHEDAMIALVTGLVVIIRLVEIIFDPIIGSIIDNTHTR
WGKFKPWLVVGGIMSSLMIMLMFSDFFGLAKSGNRTLFAIVFIIAFIILDAFYSFKDIAFWSMIPALSEKNSERETLGTF
ARFGSAIGAQGATILAIPITIFFTKGGHAQGARGFFAFGVIAALVQGISALVTAWGTKEQKSVIRQEGTKTNTSDVFKAL
LKNDQLMWLSLSYILFAIAYVATTATLILNFTFVIGNASLYSITGIVGFIGSIILVPMFPTLAKKFGRRKVLTGAIISML
VGYVLFVLGNSVPMTVGGLIFLTAPYQLVFLSVLMTITDSVEYGQWKNGVRNEAVTLAMRPLLDKIAGAFSNGIYGFVAI
AAGMTGSKYISGHTYGVATFKLYSFVVPAVLMIIALAVYLLKVKLTEKRHEEIVAELEERLK
>Mature_462_residues
MKEGKMKQRLSYAFGALGHDVYYYSISTFFIAFVTAQMFAGSPHEDAMIALVTGLVVIIRLVEIIFDPIIGSIIDNTHTR
WGKFKPWLVVGGIMSSLMIMLMFSDFFGLAKSGNRTLFAIVFIIAFIILDAFYSFKDIAFWSMIPALSEKNSERETLGTF
ARFGSAIGAQGATILAIPITIFFTKGGHAQGARGFFAFGVIAALVQGISALVTAWGTKEQKSVIRQEGTKTNTSDVFKAL
LKNDQLMWLSLSYILFAIAYVATTATLILNFTFVIGNASLYSITGIVGFIGSIILVPMFPTLAKKFGRRKVLTGAIISML
VGYVLFVLGNSVPMTVGGLIFLTAPYQLVFLSVLMTITDSVEYGQWKNGVRNEAVTLAMRPLLDKIAGAFSNGIYGFVAI
AAGMTGSKYISGHTYGVATFKLYSFVVPAVLMIIALAVYLLKVKLTEKRHEEIVAELEERLK

Specific function: Responsible for transport of beta-galactosides into the cell, with the concomitant uptake of protons (symport system), and also for transport of homologous and heterologous exchange of beta-galactosides [H]

COG id: COG2211

COG function: function code G; Na+/melibiose symporter and related transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790561, Length=474, Percent_Identity=27.0042194092827, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1787902, Length=478, Percent_Identity=23.0125523012552, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI48994989, Length=465, Percent_Identity=22.3655913978495, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI145693206, Length=469, Percent_Identity=22.1748400852878, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786466, Length=489, Percent_Identity=24.9488752556237, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87082306, Length=467, Percent_Identity=20.3426124197002, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR016196
- InterPro:   IPR001927
- InterPro:   IPR018043
- InterPro:   IPR001127 [H]

Pfam domain/function: PF00358 PTS_EIIA_1 [H]

EC number: NA

Molecular weight: Translated: 50505; Mature: 50505

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS00872 NA_GALACTOSIDE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Li (I) [Periplasm]; melibiose [Periplasm]; Na (I) [Periplasm]; melibiose [Periplasm]; Proton [Periplasm]; melibiose [Periplasm] [C]

Specific reaction: Li (I) [Periplasm] + melibiose [Periplasm] = Li (I) [Cytoplasm] + melibiose [Cytoplasm] Na (I) [Periplasm] + melibiose [Periplasm] = Na (I) [Cytoplasm] + melibiose [Cytoplasm] Proton [Periplasm] + melibiose [Periplasm] = Proton [Cytoplasm] + melibiose [Cy

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8633855 [H]