Click here to switch to the map view.

The map label for this gene is menF [H]

Identifier: 125624617

GI number: 125624617

Start: 1807726

End: 1808877

Strand: Direct

Name: menF [H]

Synonym: llmg_1828

Alternate gene names: 125624617

Gene position: 1807726-1808877 (Clockwise)

Preceding gene: 125624612

Following gene: 125624618

Centisome position: 71.47

GC content: 35.85

Gene sequence:

>1152_bases
ATGGTAGAAAAAATGAAATATATAAAAAACGATTTAATATTAAAAAACCCATTGTCCTTTTGGGCAAATTTTCCAGAAAG
TGAACGTTTCTTTTGGTTTGACCCACAAAAAGAAGAGTTTGTTATTGGTGCGGAACGTCTAAGAGCTGTTGACCCTGATA
AGGATAATTTATCTGACATTCCTTATCTTTTCCACAGTCAGACTTTTTTTGATGATGTCAAAGGAGAACGATGGAAAAAT
TTTGGGGGCGAAACGATTGCTTTCAAGCATTATTTTGTTGCCAACAAAAAAACTTCTTATTATCTCACTTGTGATCCTCC
AAAAAATATTAGCGAAATCCCTCTTACTAAGATTAAGCACCAAATCAGTGAAAAGGCTTCTGACTTTTCAGACTGGGAAG
AACTTTTTCAGGCCATTTCGCAAAATTTTACTGACGGAAAAAGTAAGAAAATTGTAGCTTCCAGAGAACTTGAATTCCTA
AGTCAAAGCCCTTTTGAGCTCGAAAGTATTCTCCAAAATCTTCTTGAAAATAATCCTAATGCTTTTATTTTTGCTTATCA
AAAAGAACAAAGAATTTTTCTTGGTGCCTCACCAGAAATCTTAGTTCAAAAAAATGGCAATCAGTTGTTGAGCTATGCTT
TAGCAGGCACCTTTCCTAAAACAATGGAAAATGCGGGCGAAAAATTACTGACAGACCCTAAAAATCTTTTAGAACATGAG
ATTGTTGTCCAAAAAATCAAGAAAAATTTGCTTGAAAAAGCCGAATCGGTCAAAGTTGGTCAGACGGGTTTAATGGAGCT
AAAAAATGTCTATCATCTTCGGACCTTATTGACCGCAAAAAGTTCTAAAAGCTCGCTCATTGATTGGGGAAAACATCTTC
ACCCAACGCCGGCATTAGGAGGTGAACCGCGACATGAAGCACTCACTTTTCTTAGAAAACATGAAAAGCATGAACGTGGA
CTTTACGCGGCTCCTCTTGGTTTGATTGATAATGAAGGCAACGGAACATTAATTGTTGGCATTCGTTCAGCTTTAATAAA
TGAGCAAAAACTTTATGCTTATGCTGGCTGTGGAATCATTCCATCTTCTGATGCTTTAGAAGAATTTAATGAAACCAAGG
TAAAATTAAAAACGATTTTAGAAGCCTTATGA

Upstream 100 bases:

>100_bases
GTTAGAAAAACTATGAAAGAAAAAGCTCTTTAATTTAAGGAACTTTTGTAATTTTGGGGCTCAATGCTCCCCTCATTTAG
TGAGCTATGTTATAATTTCT

Downstream 100 bases:

>100_bases
CTTCTATTTAGAATATCAATCCATTAGAAAAGGACACAATCATGACCAATGAATATTTAGCTCCCTTTGTTGATGAACTT
TTCAATCTCGGAGTACGTGA

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MVEKMKYIKNDLILKNPLSFWANFPESERFFWFDPQKEEFVIGAERLRAVDPDKDNLSDIPYLFHSQTFFDDVKGERWKN
FGGETIAFKHYFVANKKTSYYLTCDPPKNISEIPLTKIKHQISEKASDFSDWEELFQAISQNFTDGKSKKIVASRELEFL
SQSPFELESILQNLLENNPNAFIFAYQKEQRIFLGASPEILVQKNGNQLLSYALAGTFPKTMENAGEKLLTDPKNLLEHE
IVVQKIKKNLLEKAESVKVGQTGLMELKNVYHLRTLLTAKSSKSSLIDWGKHLHPTPALGGEPRHEALTFLRKHEKHERG
LYAAPLGLIDNEGNGTLIVGIRSALINEQKLYAYAGCGIIPSSDALEEFNETKVKLKTILEAL

Sequences:

>Translated_383_residues
MVEKMKYIKNDLILKNPLSFWANFPESERFFWFDPQKEEFVIGAERLRAVDPDKDNLSDIPYLFHSQTFFDDVKGERWKN
FGGETIAFKHYFVANKKTSYYLTCDPPKNISEIPLTKIKHQISEKASDFSDWEELFQAISQNFTDGKSKKIVASRELEFL
SQSPFELESILQNLLENNPNAFIFAYQKEQRIFLGASPEILVQKNGNQLLSYALAGTFPKTMENAGEKLLTDPKNLLEHE
IVVQKIKKNLLEKAESVKVGQTGLMELKNVYHLRTLLTAKSSKSSLIDWGKHLHPTPALGGEPRHEALTFLRKHEKHERG
LYAAPLGLIDNEGNGTLIVGIRSALINEQKLYAYAGCGIIPSSDALEEFNETKVKLKTILEAL
>Mature_383_residues
MVEKMKYIKNDLILKNPLSFWANFPESERFFWFDPQKEEFVIGAERLRAVDPDKDNLSDIPYLFHSQTFFDDVKGERWKN
FGGETIAFKHYFVANKKTSYYLTCDPPKNISEIPLTKIKHQISEKASDFSDWEELFQAISQNFTDGKSKKIVASRELEFL
SQSPFELESILQNLLENNPNAFIFAYQKEQRIFLGASPEILVQKNGNQLLSYALAGTFPKTMENAGEKLLTDPKNLLEHE
IVVQKIKKNLLEKAESVKVGQTGLMELKNVYHLRTLLTAKSSKSSLIDWGKHLHPTPALGGEPRHEALTFLRKHEKHERG
LYAAPLGLIDNEGNGTLIVGIRSALINEQKLYAYAGCGIIPSSDALEEFNETKVKLKTILEAL

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=239, Percent_Identity=33.8912133891213, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI87082077, Length=388, Percent_Identity=26.0309278350515, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787518, Length=295, Percent_Identity=23.728813559322, Blast_Score=76, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320935, Length=238, Percent_Identity=23.5294117647059, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 43655; Mature: 43655

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKMKYIKNDLILKNPLSFWANFPESERFFWFDPQKEEFVIGAERLRAVDPDKDNLSDI
CCCHHHHHHHCCEEECCHHHHCCCCCCCCEEEECCCCCCEEEEHHHHHCCCCCCCCCCCC
PYLFHSQTFFDDVKGERWKNFGGETIAFKHYFVANKKTSYYLTCDPPKNISEIPLTKIKH
CHHHCCCHHHHHHCCHHHHCCCCCEEEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHH
QISEKASDFSDWEELFQAISQNFTDGKSKKIVASRELEFLSQSPFELESILQNLLENNPN
HHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
AFIFAYQKEQRIFLGASPEILVQKNGNQLLSYALAGTFPKTMENAGEKLLTDPKNLLEHE
EEEEEEECCCEEEECCCCCEEEECCCCHHHHHHHHCCCHHHHHHCCHHHHCCHHHHHHHH
IVVQKIKKNLLEKAESVKVGQTGLMELKNVYHLRTLLTAKSSKSSLIDWGKHLHPTPALG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
GEPRHEALTFLRKHEKHERGLYAAPLGLIDNEGNGTLIVGIRSALINEQKLYAYAGCGII
CCCHHHHHHHHHHHHHHHCCCEECCCCEEECCCCCEEEEEEHHHHHCCHHEEEEECCCCC
PSSDALEEFNETKVKLKTILEAL
CCHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MVEKMKYIKNDLILKNPLSFWANFPESERFFWFDPQKEEFVIGAERLRAVDPDKDNLSDI
CCCHHHHHHHCCEEECCHHHHCCCCCCCCEEEECCCCCCEEEEHHHHHCCCCCCCCCCCC
PYLFHSQTFFDDVKGERWKNFGGETIAFKHYFVANKKTSYYLTCDPPKNISEIPLTKIKH
CHHHCCCHHHHHHCCHHHHCCCCCEEEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHH
QISEKASDFSDWEELFQAISQNFTDGKSKKIVASRELEFLSQSPFELESILQNLLENNPN
HHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
AFIFAYQKEQRIFLGASPEILVQKNGNQLLSYALAGTFPKTMENAGEKLLTDPKNLLEHE
EEEEEEECCCEEEECCCCCEEEECCCCHHHHHHHHCCCHHHHHHCCHHHHCCHHHHHHHH
IVVQKIKKNLLEKAESVKVGQTGLMELKNVYHLRTLLTAKSSKSSLIDWGKHLHPTPALG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
GEPRHEALTFLRKHEKHERGLYAAPLGLIDNEGNGTLIVGIRSALINEQKLYAYAGCGII
CCCHHHHHHHHHHHHHHHCCCEECCCCEEECCCCCEEEEEEHHHHHCCHHEEEEECCCCC
PSSDALEEFNETKVKLKTILEAL
CCHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]