Click here to switch to the map view.

The map label for this gene is ansB [H]

Identifier: 125624612

GI number: 125624612

Start: 1803517

End: 1804485

Strand: Direct

Name: ansB [H]

Synonym: llmg_1823

Alternate gene names: 125624612

Gene position: 1803517-1804485 (Clockwise)

Preceding gene: 125624609

Following gene: 125624617

Centisome position: 71.3

GC content: 38.39

Gene sequence:

>969_bases
ATGAAAAAAATCTTAATTCTCCACACTGGTGGAACAATTTCGATGGCGGAAGATGAAAATGGTCATGTTTCCCCAAATGC
AACAAATCCAATGAATGCTGTGAATATGCAATTTGATGAAATCGACTTGGTAACAGAAGATATTTTTAATCTTCCAAGCC
CCCATGTTACTTTAACAGATATGTTGGTTTTAAAAAATAGAATTAAAAAGGCATTTATTTCTGAGAATTTTGATGGTGTG
GTGATTACTCATGGAACTGACACCCTTGAAGAAACAGCCTTTTTCCTTGATACGACTCTTTCTCGTGGCAAACCGATTGT
CCTAACAGGTGCTATGCGCTCAAGTAATGAATTGGGGACTGACGGAATTTATAATTTACTGACAGCCATTCGTGTTGCTG
CTGACAGTAATTCAAGTGACCGTGGGGTACTTGTGGTAATGAATGACGAAATTCATTCTGCTCGTTATGTCACTAAAACA
CACACGACTAACGTGTCAACTTTCCAAACTCCGACGCATGGTCCTGTCGGTTTACTGACCAAAAATAAAATTATCTATTT
CCACAAAGATAGTGAAAATCAACATTTGGATGTGGCAACAGTTCATGGAAATGTACCGATCGTCAAGGCTTTTGCCGGAA
TGACTGGTGAAATTTTAGATTTATTAGATTTAGAGAAGTTAGACGGAATTGTCATTGAAGCATTAGGTGCTGGAAATCTC
CCACCTATTGCTAGCCAATCACTGCAAAGACTCTTGGACGCTAAGGTTCCTGTTGTTCTTGTTTCTCGTTGTTTCAATGG
GATTGCCGAACCAGTCTATTCTTATCAGGGTGGCGGTTCACAACTTTACGAAGCTGGTGTGATGTTTGCTGACGAAGTGA
ATTCTCAAAAGGCACGAATTAAATTGATTATTGCCTTAAATGCTGGTTTGAAAAATTTATCAGAATTACGTCCTTTTTTG
GAAAATTAA

Upstream 100 bases:

>100_bases
TGGAGTAAATTTTGATATAATGCTAGTGCTGGCTTTTATATGAAGTCTATAAATTTTTATATTTAAATTTTGTTTATTAT
AGATAGATAAGGGAATAATG

Downstream 100 bases:

>100_bases
ATAAAAATTACTGACAAAAGAAAGAATCATCAGCTGACTGATGATTCTTTTATTTGCCGATTTAAAGCTCATTATTCAAT
CGCTTTAAACTGTCAGTAGA

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKKILILHTGGTISMAEDENGHVSPNATNPMNAVNMQFDEIDLVTEDIFNLPSPHVTLTDMLVLKNRIKKAFISENFDGV
VITHGTDTLEETAFFLDTTLSRGKPIVLTGAMRSSNELGTDGIYNLLTAIRVAADSNSSDRGVLVVMNDEIHSARYVTKT
HTTNVSTFQTPTHGPVGLLTKNKIIYFHKDSENQHLDVATVHGNVPIVKAFAGMTGEILDLLDLEKLDGIVIEALGAGNL
PPIASQSLQRLLDAKVPVVLVSRCFNGIAEPVYSYQGGGSQLYEAGVMFADEVNSQKARIKLIIALNAGLKNLSELRPFL
EN

Sequences:

>Translated_322_residues
MKKILILHTGGTISMAEDENGHVSPNATNPMNAVNMQFDEIDLVTEDIFNLPSPHVTLTDMLVLKNRIKKAFISENFDGV
VITHGTDTLEETAFFLDTTLSRGKPIVLTGAMRSSNELGTDGIYNLLTAIRVAADSNSSDRGVLVVMNDEIHSARYVTKT
HTTNVSTFQTPTHGPVGLLTKNKIIYFHKDSENQHLDVATVHGNVPIVKAFAGMTGEILDLLDLEKLDGIVIEALGAGNL
PPIASQSLQRLLDAKVPVVLVSRCFNGIAEPVYSYQGGGSQLYEAGVMFADEVNSQKARIKLIIALNAGLKNLSELRPFL
EN
>Mature_322_residues
MKKILILHTGGTISMAEDENGHVSPNATNPMNAVNMQFDEIDLVTEDIFNLPSPHVTLTDMLVLKNRIKKAFISENFDGV
VITHGTDTLEETAFFLDTTLSRGKPIVLTGAMRSSNELGTDGIYNLLTAIRVAADSNSSDRGVLVVMNDEIHSARYVTKT
HTTNVSTFQTPTHGPVGLLTKNKIIYFHKDSENQHLDVATVHGNVPIVKAFAGMTGEILDLLDLEKLDGIVIEALGAGNL
PPIASQSLQRLLDAKVPVVLVSRCFNGIAEPVYSYQGGGSQLYEAGVMFADEVNSQKARIKLIIALNAGLKNLSELRPFL
EN

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=299, Percent_Identity=23.7458193979933, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789327, Length=284, Percent_Identity=36.2676056338028, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1788065, Length=321, Percent_Identity=26.1682242990654, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=359, Percent_Identity=25.3481894150418, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI193207374, Length=359, Percent_Identity=25.3481894150418, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17558278, Length=359, Percent_Identity=25.3481894150418, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17558280, Length=359, Percent_Identity=25.3481894150418, Blast_Score=95, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320527, Length=331, Percent_Identity=32.6283987915408, Blast_Score=139, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6323189, Length=325, Percent_Identity=29.5384615384615, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323187, Length=325, Percent_Identity=29.5384615384615, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323186, Length=325, Percent_Identity=29.5384615384615, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323184, Length=325, Percent_Identity=29.5384615384615, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24645530, Length=340, Percent_Identity=25.5882352941176, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24639609, Length=339, Percent_Identity=21.5339233038348, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 34966; Mature: 34966

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILILHTGGTISMAEDENGHVSPNATNPMNAVNMQFDEIDLVTEDIFNLPSPHVTLTD
CCEEEEEECCCEEEEECCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCEEHHH
MLVLKNRIKKAFISENFDGVVITHGTDTLEETAFFLDTTLSRGKPIVLTGAMRSSNELGT
HHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
DGIYNLLTAIRVAADSNSSDRGVLVVMNDEIHSARYVTKTHTTNVSTFQTPTHGPVGLLT
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCEEECCCCCCCEEEEE
KNKIIYFHKDSENQHLDVATVHGNVPIVKAFAGMTGEILDLLDLEKLDGIVIEALGAGNL
CCEEEEEEECCCCCEEEEEEEECCCCEEEHHCCCCHHHHHHHHHHHHCCEEEEECCCCCC
PPIASQSLQRLLDAKVPVVLVSRCFNGIAEPVYSYQGGGSQLYEAGVMFADEVNSQKARI
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCEEEECCCCCCCEEE
KLIIALNAGLKNLSELRPFLEN
EEEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKILILHTGGTISMAEDENGHVSPNATNPMNAVNMQFDEIDLVTEDIFNLPSPHVTLTD
CCEEEEEECCCEEEEECCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCEEHHH
MLVLKNRIKKAFISENFDGVVITHGTDTLEETAFFLDTTLSRGKPIVLTGAMRSSNELGT
HHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
DGIYNLLTAIRVAADSNSSDRGVLVVMNDEIHSARYVTKTHTTNVSTFQTPTHGPVGLLT
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCEEECCCCCCCEEEEE
KNKIIYFHKDSENQHLDVATVHGNVPIVKAFAGMTGEILDLLDLEKLDGIVIEALGAGNL
CCEEEEEEECCCCCEEEEEEEECCCCEEEHHCCCCHHHHHHHHHHHHCCEEEEECCCCCC
PPIASQSLQRLLDAKVPVVLVSRCFNGIAEPVYSYQGGGSQLYEAGVMFADEVNSQKARI
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCEEEECCCCCCCEEE
KLIIALNAGLKNLSELRPFLEN
EEEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]