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The map label for this gene is menD

Identifier: 125624618

GI number: 125624618

Start: 1808919

End: 1810583

Strand: Direct

Name: menD

Synonym: llmg_1829

Alternate gene names: 125624618

Gene position: 1808919-1810583 (Clockwise)

Preceding gene: 125624617

Following gene: 125624619

Centisome position: 71.51

GC content: 39.16

Gene sequence:

>1665_bases
ATGACCAATGAATATTTAGCTCCCTTTGTTGATGAACTTTTCAATCTCGGAGTACGTGAAGCTGTCTTTAGCCCTGGCTC
ACGTTCAACGGCTTTAGCCATGCTTTTTGAAGAATATAAAAAATATGACACTTATGTCAATATTGATGAACGTTCGGCCG
CTTTTTTTGCACTCGGAATTGCTAAGGCAAATCGTCGACCAGTTGTGCTGGTTTGTACTTCTGGCTCTGCTGCTGCTCAT
CATTTTCCAGCTATTACTGAAGCAAAGACGAGTCGTATCCCACTCATTATTTTGACAGCTGACCGTCCAGCCGAGCTTCA
ATTTGTTGGAGCGCCTCAAACGCTTGACCAAACCAGATTTTTTGGAAATTTCGTCAATCATTTTGAAAATCTTGAAGCCC
CCCAGCCTCAAGCAAAAAACTTTTGGACTTATCCGCGAAAAGTGGCTCAACGCGCCTTTTTATCTGCCCTTGACCAGATG
GCTGGACCTGTTCAAATCAACATTCCATTGCGCGACCCTTTAGTTCCCGAATTAAAGAGTGAAAACTATGAAAAAGGACG
TTCTAAACTGCCTTTTAAATTCTTTAAAGGTCAACAAAGCGCCTCTTTTGATGAAGCATTACTTTCTTCAAAAACACTGA
TTTTGGCAGGAGCCAATTCTTCTGAAAATTATTCAGAGTCACTTTTAAAACTTGCCGAACACTTAAAAGCTCCCATCTTG
GCAGACCCTCTTTCCAATCTTAGAAATCACAATTCTCCTTTTGTCATGGACAGCTATGATGCTTTTTTGGCAAATGATGA
CTTGAAAACTGACTTAAAAGCGGAGTCTATTCTCCTTTTTGGTCAGATGCCTGTTTCTAAAAGATTACAACAATTTATTG
CCCTCAATGACGATGCAGAATTTATTCAAGTAGACCCTGCTTTAGCTTATCGCAATCCAAGTTTAACAACGACAATCACG
GTCCAATCCAATGTCGCAACTTTTGCAAATTCTATCCAAAAAGTGAATCAAGACTTTTCATATTTGGAGAAATGGCAAAA
GGCCCAAGAAAAAATGCGTCATCAACTTGAAAAAGTGGCTCAGGAAGAAAATCCATTTGAAGGACGTTTCGTCCAAGAAT
TACAAAAACATTTGAAAGCACTTGACGCTCAACTTCTTGTTTCAAATAGTATGGAAATCCGCGATATAGATTATTGGTGG
AAAAAGGAAGATTCCAAGGTCAGAATCTTAGGAAATCGTGGTGTCAATGGCATTGACGGAACCGAATCTACAGCGCTTGG
TATTGCTACAACCGGAAAACCAACGGTTTTACTGACAGGCGATTTGTCCATGCTCCATGACTTAAATGGGCTAATTATTG
GTAAAACGCATGAACTTAATCTGACAATCGTTCTTTTCAATAATGATGGTGGCGGAATTTTTCATCATTTAGCGCAAAAA
GGCGTGCCAAATTTTGATTATCTCTTTTCAACCCCGCATGGCTTGAATTTTGAGGGCTTGGCTGAATTGACAGGTTTAGA
TTATCATTTGGTCAGTAATTATGCTGATTTTGGTCAACAATTTGAAACTTCTATCCGTCAACCTGGCATTCATCTTTTGG
AAATTAAAACGGACAAAGATTTAAGTTTGGCTTTGCATAAAAAATATACTGCTTATGAAAATTGA

Upstream 100 bases:

>100_bases
CTTTAGAAGAATTTAATGAAACCAAGGTAAAATTAAAAACGATTTTAGAAGCCTTATGACTTCTATTTAGAATATCAATC
CATTAGAAAAGGACACAATC

Downstream 100 bases:

>100_bases
TAAAAAAATAATGACGATTGCGGGTCTTGATTATGCTGTCAGTATTTGGGGAAGTGGGAAAATTATTTTTGCATTGCATG
GTTTTTCCGAAAGTTCAAGC

Product: 2-oxoglutarate decarboxylase / 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MTNEYLAPFVDELFNLGVREAVFSPGSRSTALAMLFEEYKKYDTYVNIDERSAAFFALGIAKANRRPVVLVCTSGSAAAH
HFPAITEAKTSRIPLIILTADRPAELQFVGAPQTLDQTRFFGNFVNHFENLEAPQPQAKNFWTYPRKVAQRAFLSALDQM
AGPVQINIPLRDPLVPELKSENYEKGRSKLPFKFFKGQQSASFDEALLSSKTLILAGANSSENYSESLLKLAEHLKAPIL
ADPLSNLRNHNSPFVMDSYDAFLANDDLKTDLKAESILLFGQMPVSKRLQQFIALNDDAEFIQVDPALAYRNPSLTTTIT
VQSNVATFANSIQKVNQDFSYLEKWQKAQEKMRHQLEKVAQEENPFEGRFVQELQKHLKALDAQLLVSNSMEIRDIDYWW
KKEDSKVRILGNRGVNGIDGTESTALGIATTGKPTVLLTGDLSMLHDLNGLIIGKTHELNLTIVLFNNDGGGIFHHLAQK
GVPNFDYLFSTPHGLNFEGLAELTGLDYHLVSNYADFGQQFETSIRQPGIHLLEIKTDKDLSLALHKKYTAYEN

Sequences:

>Translated_554_residues
MTNEYLAPFVDELFNLGVREAVFSPGSRSTALAMLFEEYKKYDTYVNIDERSAAFFALGIAKANRRPVVLVCTSGSAAAH
HFPAITEAKTSRIPLIILTADRPAELQFVGAPQTLDQTRFFGNFVNHFENLEAPQPQAKNFWTYPRKVAQRAFLSALDQM
AGPVQINIPLRDPLVPELKSENYEKGRSKLPFKFFKGQQSASFDEALLSSKTLILAGANSSENYSESLLKLAEHLKAPIL
ADPLSNLRNHNSPFVMDSYDAFLANDDLKTDLKAESILLFGQMPVSKRLQQFIALNDDAEFIQVDPALAYRNPSLTTTIT
VQSNVATFANSIQKVNQDFSYLEKWQKAQEKMRHQLEKVAQEENPFEGRFVQELQKHLKALDAQLLVSNSMEIRDIDYWW
KKEDSKVRILGNRGVNGIDGTESTALGIATTGKPTVLLTGDLSMLHDLNGLIIGKTHELNLTIVLFNNDGGGIFHHLAQK
GVPNFDYLFSTPHGLNFEGLAELTGLDYHLVSNYADFGQQFETSIRQPGIHLLEIKTDKDLSLALHKKYTAYEN
>Mature_553_residues
TNEYLAPFVDELFNLGVREAVFSPGSRSTALAMLFEEYKKYDTYVNIDERSAAFFALGIAKANRRPVVLVCTSGSAAAHH
FPAITEAKTSRIPLIILTADRPAELQFVGAPQTLDQTRFFGNFVNHFENLEAPQPQAKNFWTYPRKVAQRAFLSALDQMA
GPVQINIPLRDPLVPELKSENYEKGRSKLPFKFFKGQQSASFDEALLSSKTLILAGANSSENYSESLLKLAEHLKAPILA
DPLSNLRNHNSPFVMDSYDAFLANDDLKTDLKAESILLFGQMPVSKRLQQFIALNDDAEFIQVDPALAYRNPSLTTTITV
QSNVATFANSIQKVNQDFSYLEKWQKAQEKMRHQLEKVAQEENPFEGRFVQELQKHLKALDAQLLVSNSMEIRDIDYWWK
KEDSKVRILGNRGVNGIDGTESTALGIATTGKPTVLLTGDLSMLHDLNGLIIGKTHELNLTIVLFNNDGGGIFHHLAQKG
VPNFDYLFSTPHGLNFEGLAELTGLDYHLVSNYADFGQQFETSIRQPGIHLLEIKTDKDLSLALHKKYTAYEN

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=553, Percent_Identity=27.6672694394213, Blast_Score=178, Evalue=7e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_LACLM (A2RM73)

Other databases:

- EMBL:   AM406671
- RefSeq:   YP_001033101.1
- ProteinModelPortal:   A2RM73
- STRING:   A2RM73
- GeneID:   4797539
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_1829
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   CLSK697379
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 62051; Mature: 61920

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNEYLAPFVDELFNLGVREAVFSPGSRSTALAMLFEEYKKYDTYVNIDERSAAFFALGI
CCCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEE
AKANRRPVVLVCTSGSAAAHHFPAITEAKTSRIPLIILTADRPAELQFVGAPQTLDQTRF
CCCCCCCEEEEECCCCCCHHCCCCHHCCCCCCCCEEEEECCCCCCEEEECCCCHHHHHHH
FGNFVNHFENLEAPQPQAKNFWTYPRKVAQRAFLSALDQMAGPVQINIPLRDPLVPELKS
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
ENYEKGRSKLPFKFFKGQQSASFDEALLSSKTLILAGANSSENYSESLLKLAEHLKAPIL
CCHHHHCCCCCHHHHCCCCCCCHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCH
ADPLSNLRNHNSPFVMDSYDAFLANDDLKTDLKAESILLFGQMPVSKRLQQFIALNDDAE
HHHHHHHHCCCCCEEEECCHHHEECCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCC
FIQVDPALAYRNPSLTTTITVQSNVATFANSIQKVNQDFSYLEKWQKAQEKMRHQLEKVA
EEEECCHHEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEENPFEGRFVQELQKHLKALDAQLLVSNSMEIRDIDYWWKKEDSKVRILGNRGVNGIDG
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHCCCCCEEEEEECCCCCCCCC
TESTALGIATTGKPTVLLTGDLSMLHDLNGLIIGKTHELNLTIVLFNNDGGGIFHHLAQK
CCCCEEEEEECCCCEEEEECCHHHHHCCCCEEEEEEEEEEEEEEEEECCCCHHHHHHHHC
GVPNFDYLFSTPHGLNFEGLAELTGLDYHLVSNYADFGQQFETSIRQPGIHLLEIKTDKD
CCCCCCCEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
LSLALHKKYTAYEN
CEEEEHHCHHCCCC
>Mature Secondary Structure 
TNEYLAPFVDELFNLGVREAVFSPGSRSTALAMLFEEYKKYDTYVNIDERSAAFFALGI
CCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEE
AKANRRPVVLVCTSGSAAAHHFPAITEAKTSRIPLIILTADRPAELQFVGAPQTLDQTRF
CCCCCCCEEEEECCCCCCHHCCCCHHCCCCCCCCEEEEECCCCCCEEEECCCCHHHHHHH
FGNFVNHFENLEAPQPQAKNFWTYPRKVAQRAFLSALDQMAGPVQINIPLRDPLVPELKS
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
ENYEKGRSKLPFKFFKGQQSASFDEALLSSKTLILAGANSSENYSESLLKLAEHLKAPIL
CCHHHHCCCCCHHHHCCCCCCCHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCH
ADPLSNLRNHNSPFVMDSYDAFLANDDLKTDLKAESILLFGQMPVSKRLQQFIALNDDAE
HHHHHHHHCCCCCEEEECCHHHEECCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCC
FIQVDPALAYRNPSLTTTITVQSNVATFANSIQKVNQDFSYLEKWQKAQEKMRHQLEKVA
EEEECCHHEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEENPFEGRFVQELQKHLKALDAQLLVSNSMEIRDIDYWWKKEDSKVRILGNRGVNGIDG
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHCCCCCEEEEEECCCCCCCCC
TESTALGIATTGKPTVLLTGDLSMLHDLNGLIIGKTHELNLTIVLFNNDGGGIFHHLAQK
CCCCEEEEEECCCCEEEEECCHHHHHCCCCEEEEEEEEEEEEEEEEECCCCHHHHHHHHC
GVPNFDYLFSTPHGLNFEGLAELTGLDYHLVSNYADFGQQFETSIRQPGIHLLEIKTDKD
CCCCCCCEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
LSLALHKKYTAYEN
CEEEEHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA