Click here to switch to the map view.

The map label for this gene is hisZ

Identifier: 125624119

GI number: 125624119

Start: 1266502

End: 1267458

Strand: Reverse

Name: hisZ

Synonym: llmg_1297

Alternate gene names: 125624119

Gene position: 1267458-1266502 (Counterclockwise)

Preceding gene: 125624120

Following gene: 125624118

Centisome position: 50.11

GC content: 42.74

Gene sequence:

>957_bases
ATGAAAAAAATGAACTATTTGCTCCCCGAAGAATCGGGTGAAATGACATTGTCAGGAATTACGACACTTCGCAAGATAGA
ACAGAAGTTGCGGAACTTGTTTGAAAGTCAAAATTATCAGGAGGTCATGCCACCGAATTTTGAATATGTCGAGCTTTATA
CTGGCCTTGACGCAGGATTTGAGCAAGAGAAGATGTTTCAATTTATCAATCATGAGGGTAAATCGATTGCGCTTCGTTAT
GATTTTACAGTCCCTTTGGCACGAAATTTTGCCTTATCAGAGCTGACAGAAGCCCGGTATAGTTATTTTGGTAAGGTGTT
TCGTAAAGAAAAGCGTCATAAGGGACGTCGGACGGAGAGCTATCAGGTCGGGACAGAATTGTTAGGATTGTCAGAAGTGA
CAGGAGATCAGGAGATTTTAGGACTGACTTTTATGAGCTTAGAGGCTCTAACATTGAAAAACACTATCGTAGAAATTGGT
TCGGCTGCTTTTTATAAGCGTTTATGTGAGCTGTCAGGTGGAGATGCGCAACTTTTTTCGGAACTTCTTGAGAAAAAATC
ACTCTCAGGTATGAAAGCTTTCGTGGACAAGCATGAAATGATAGGCGCGCCACGCGACTTGTTGCTTGCTTTGATGACCA
CGACCGATTTGCCAACCATGAAAAAATTAGTTTTGGCAACAGGCGATGAAAAATTGTCTCAGGCACTCGAAATGCTTGAA
GCGCTGAATTTACCTGACAAGACAGCGATTTGCCAGATTCACTATGATTTTGCCATGGTACCTGCGATGGGCTACTATAC
TGGGCTGATGTTTCAGGTTTACGTAGAAGGCGTGGCGCAAGCGACTATATCCGGTGGGCGCTATGACAAGTTACTAAAAC
AGTTCGGTAAAACCACGGGCTCAATTGGCTTTTGCGTGCATATGGACAATGTTGTGAAAGGGTTGAATAATGATTAG

Upstream 100 bases:

>100_bases
TTTCTGATTGGTTACGGATTACGATTGGCACTGATAGAGAAATGAACACAGTGATTCAATTCTTAAAGGAATATTTAAAA
AACAAGGAGAAAAGTTACTG

Downstream 100 bases:

>100_bases
AATAGCAATGACCAATGGCTGGAAGCGACCAAGAGGGAGCTTTCAGGTTGCGGATAGAAGCTTGCGCAAGCAAGCTGTCA
AAGGCGACCAAGGGGGAGCT

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKKMNYLLPEESGEMTLSGITTLRKIEQKLRNLFESQNYQEVMPPNFEYVELYTGLDAGFEQEKMFQFINHEGKSIALRY
DFTVPLARNFALSELTEARYSYFGKVFRKEKRHKGRRTESYQVGTELLGLSEVTGDQEILGLTFMSLEALTLKNTIVEIG
SAAFYKRLCELSGGDAQLFSELLEKKSLSGMKAFVDKHEMIGAPRDLLLALMTTTDLPTMKKLVLATGDEKLSQALEMLE
ALNLPDKTAICQIHYDFAMVPAMGYYTGLMFQVYVEGVAQATISGGRYDKLLKQFGKTTGSIGFCVHMDNVVKGLNND

Sequences:

>Translated_318_residues
MKKMNYLLPEESGEMTLSGITTLRKIEQKLRNLFESQNYQEVMPPNFEYVELYTGLDAGFEQEKMFQFINHEGKSIALRY
DFTVPLARNFALSELTEARYSYFGKVFRKEKRHKGRRTESYQVGTELLGLSEVTGDQEILGLTFMSLEALTLKNTIVEIG
SAAFYKRLCELSGGDAQLFSELLEKKSLSGMKAFVDKHEMIGAPRDLLLALMTTTDLPTMKKLVLATGDEKLSQALEMLE
ALNLPDKTAICQIHYDFAMVPAMGYYTGLMFQVYVEGVAQATISGGRYDKLLKQFGKTTGSIGFCVHMDNVVKGLNND
>Mature_318_residues
MKKMNYLLPEESGEMTLSGITTLRKIEQKLRNLFESQNYQEVMPPNFEYVELYTGLDAGFEQEKMFQFINHEGKSIALRY
DFTVPLARNFALSELTEARYSYFGKVFRKEKRHKGRRTESYQVGTELLGLSEVTGDQEILGLTFMSLEALTLKNTIVEIG
SAAFYKRLCELSGGDAQLFSELLEKKSLSGMKAFVDKHEMIGAPRDLLLALMTTTDLPTMKKLVLATGDEKLSQALEMLE
ALNLPDKTAICQIHYDFAMVPAMGYYTGLMFQVYVEGVAQATISGGRYDKLLKQFGKTTGSIGFCVHMDNVVKGLNND

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Homo sapiens, GI6996014, Length=314, Percent_Identity=23.8853503184713, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI15029520, Length=351, Percent_Identity=22.7920227920228, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6325290, Length=342, Percent_Identity=21.0526315789474, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24643061, Length=340, Percent_Identity=22.0588235294118, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_LACLM (A2RKS4)

Other databases:

- EMBL:   AM406671
- RefSeq:   YP_001032602.1
- ProteinModelPortal:   A2RKS4
- STRING:   A2RKS4
- GeneID:   4798491
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_1297
- eggNOG:   COG3705
- HOGENOM:   HBG517872
- OMA:   YREHVKS
- ProtClustDB:   CLSK697628
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 35834; Mature: 35834

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMNYLLPEESGEMTLSGITTLRKIEQKLRNLFESQNYQEVMPPNFEYVELYTGLDAGF
CCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCC
EQEKMFQFINHEGKSIALRYDFTVPLARNFALSELTEARYSYFGKVFRKEKRHKGRRTES
CHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
YQVGTELLGLSEVTGDQEILGLTFMSLEALTLKNTIVEIGSAAFYKRLCELSGGDAQLFS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ELLEKKSLSGMKAFVDKHEMIGAPRDLLLALMTTTDLPTMKKLVLATGDEKLSQALEMLE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHH
ALNLPDKTAICQIHYDFAMVPAMGYYTGLMFQVYVEGVAQATISGGRYDKLLKQFGKTTG
HCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
SIGFCVHMDNVVKGLNND
CEEEEEEHHHHHHCCCCC
>Mature Secondary Structure
MKKMNYLLPEESGEMTLSGITTLRKIEQKLRNLFESQNYQEVMPPNFEYVELYTGLDAGF
CCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCC
EQEKMFQFINHEGKSIALRYDFTVPLARNFALSELTEARYSYFGKVFRKEKRHKGRRTES
CHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
YQVGTELLGLSEVTGDQEILGLTFMSLEALTLKNTIVEIGSAAFYKRLCELSGGDAQLFS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ELLEKKSLSGMKAFVDKHEMIGAPRDLLLALMTTTDLPTMKKLVLATGDEKLSQALEMLE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHH
ALNLPDKTAICQIHYDFAMVPAMGYYTGLMFQVYVEGVAQATISGGRYDKLLKQFGKTTG
HCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
SIGFCVHMDNVVKGLNND
CEEEEEEHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA