Click here to switch to the map view.

The map label for this gene is topA [H]

Identifier: 125624096

GI number: 125624096

Start: 1240852

End: 1242978

Strand: Reverse

Name: topA [H]

Synonym: llmg_1272

Alternate gene names: 125624096

Gene position: 1242978-1240852 (Counterclockwise)

Preceding gene: 125624097

Following gene: 125624095

Centisome position: 49.14

GC content: 38.03

Gene sequence:

>2127_bases
ATGCCAACTTCAACTAAAACTAAAACTAGTACAAAGAAAAAAACAACACGAAAGAAAGTGACTCCTGGGAAAAATCTCGT
AATTGTCGAATCTCCTGCTAAGGCCAAAACAATTGAAAAATATCTTGGACGAAACTATAAAGTTGTTGCTTCTGTCGGTC
ATATTCGTGATTTAAAAAAATCAACGATGTCTGTTGATATCGAGAATGATTATGAACCACAATATATCAATATTCGAGGT
AAGGCACCTCTAATCAACTCACTCAAAAAAGAAGCCAAAGCTGCCAAAGCTGTCTATCTCGCGAGTGACCCGGACCGTGA
AGGAGAAGCAATTTCATGGCACTTAGCTCACATCTTGAATCTTCCACTCGAAGAAAAAAACCGTGTGGTCTTCAACGAAA
TTACTAAAGACGCCGTAAAAAACGCCTTTAAGGAACCACGTCAAATTGATGTAGATTTGGTTGATGCCCAACAAGCACGT
CGCGTTCTTGACCGCCTAGTTGGATATTCAATCAGTCCAATTCTTTGGAAAAAGGTCAAAAAAGGACTTTCTGCAGGTCG
AGTTCAATCGATTGCTTTAAAATTAATCGTTGACCGCGAAAATGAAATTAATGCCTTTATCCCAGAAGAATACTGGTCAA
TTGATGGGGAATTCAAAAAAGGAACCAAGAAATTTAAAGCTTCATTTTGGGGAATTGATGGAAAAAAACATCCATTAAAA
AATAACGAAGATGTTATTAAAGTGATGGAGCGTTTAGACGGTCCTGATTTCAATGTCGATAAAGTTGAAAAGAAAGAACG
TCGTAGAAATTCACCACTCCCTTATACAACAAGTTCAATGCAACAAGATGCAGCAAATAAGCTTAACTTTCGTACCCGTA
AAACAATGATGGTTGCCCAACAGCTTTATGAAGGATTGACACTCGGAAGTCAAGGACATCAAGGTCTGATTACCTATATG
CGTACCGACTCAACACGTATTTCGCCAGTCGCACAAAGTGCAGCTCATAGCTATATTGCGGAACAATTTGGCGAAAAGTA
CAGCAAGCACGGTTCAAAAGTTAAGAATGCCTCTGGTGCTCAAGATGCCCACGAAGCCATTCGACCTTCAAATGTCTTTA
ATACACCTGAGTCCATTGCTAAATATTTGGACAAAGACCAATTAAAACTCTATACACTTATTTGGAATCGTTTTGTTGCT
AGCCAAATGGTTGCAGCCATTTTTGATACTGTGAAAGTGAACCTTTCACAAAATGGTGTCATTTTTATCGCCAATGGTTC
ACAGGTCAAATTCGACGGTTATCTTAGAATCTACAACGACTCCGATAAATCAAATATGCTTCCAGAAATGGAAGAAAACG
ATACAGTGAAAAAAGCAAATGTTAAACCAGAACAACATTTCACACAACCACCGGCTAGATATTCTGAAGCAACACTCATT
AAAACATTAGAAGAAAACGGTGTAGGCCGTCCCTCAACCTATGCTCCAACCTTAGAAACAATTCAAAAACGCTATTATGT
AATATTATCAGCAAAACGATTTGAACCTACGGAACTTGGAGAAATCGTTAACAAGTTGATTGTTGAATTTTTCCCTAACA
TTGTTAATACCGAATTCACTGCCGAAATGGAAAAAGATCTTGATCAGGTTGAAGAAGGAAAACGCCAATGGATTGAGGTT
GTTGACCAATTCTACAAACCTTTTGCTAAAGAACTTGAAAATGCTGAAGAAGGTATGGAGAAGATTCAAATCAAGGATGA
ACCCGCTGGTTTTGATTGTGATGTGTGTGGAAGTCCAATGGTCATTAAATTAGGACGTTTTGGTAAGTTTTATGCTTGTA
GTAACTTCCCAGATTGCCGAAATACCAAGGCTATCGTGAAAGAAATTGGCGTAAAATGTCCACTTTGTCATGAAGGAAAC
ATCATTGAACGTAAAACTAAGAAAAATAGAATTTTCTATGGTTGTGACCGCTATCCAGAATGTGAATTTACAAGCTGGGA
CAAACCAATTGGACGTGATTGTCCTAAATCTGGTCATTTTCTCGTTGAAAAGAAAGTCCGTGGTGGTGGTAAACAAGTCG
TGTGTTCAAATGACGAGTGTGACTATCAAGAAGAAAAACAAAAATAA

Upstream 100 bases:

>100_bases
TTAGTTCAATTTCCACTTTCTAATCACTAAATCCTCTATGAACTGTATAACATTTTATAGATAGTGGATATTTAAAAATA
GATAAATAGGAATATTAATT

Downstream 100 bases:

>100_bases
AAGGCGCAATAGCGCTTTTTTATTATATTAATCAATGGTAAAATTCAATAAAATTAGATATAATTGGGACTATGAGAAAA
ACACATATTAATGTTATCGG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 708; Mature: 707

Protein sequence:

>708_residues
MPTSTKTKTSTKKKTTRKKVTPGKNLVIVESPAKAKTIEKYLGRNYKVVASVGHIRDLKKSTMSVDIENDYEPQYINIRG
KAPLINSLKKEAKAAKAVYLASDPDREGEAISWHLAHILNLPLEEKNRVVFNEITKDAVKNAFKEPRQIDVDLVDAQQAR
RVLDRLVGYSISPILWKKVKKGLSAGRVQSIALKLIVDRENEINAFIPEEYWSIDGEFKKGTKKFKASFWGIDGKKHPLK
NNEDVIKVMERLDGPDFNVDKVEKKERRRNSPLPYTTSSMQQDAANKLNFRTRKTMMVAQQLYEGLTLGSQGHQGLITYM
RTDSTRISPVAQSAAHSYIAEQFGEKYSKHGSKVKNASGAQDAHEAIRPSNVFNTPESIAKYLDKDQLKLYTLIWNRFVA
SQMVAAIFDTVKVNLSQNGVIFIANGSQVKFDGYLRIYNDSDKSNMLPEMEENDTVKKANVKPEQHFTQPPARYSEATLI
KTLEENGVGRPSTYAPTLETIQKRYYVILSAKRFEPTELGEIVNKLIVEFFPNIVNTEFTAEMEKDLDQVEEGKRQWIEV
VDQFYKPFAKELENAEEGMEKIQIKDEPAGFDCDVCGSPMVIKLGRFGKFYACSNFPDCRNTKAIVKEIGVKCPLCHEGN
IIERKTKKNRIFYGCDRYPECEFTSWDKPIGRDCPKSGHFLVEKKVRGGGKQVVCSNDECDYQEEKQK

Sequences:

>Translated_708_residues
MPTSTKTKTSTKKKTTRKKVTPGKNLVIVESPAKAKTIEKYLGRNYKVVASVGHIRDLKKSTMSVDIENDYEPQYINIRG
KAPLINSLKKEAKAAKAVYLASDPDREGEAISWHLAHILNLPLEEKNRVVFNEITKDAVKNAFKEPRQIDVDLVDAQQAR
RVLDRLVGYSISPILWKKVKKGLSAGRVQSIALKLIVDRENEINAFIPEEYWSIDGEFKKGTKKFKASFWGIDGKKHPLK
NNEDVIKVMERLDGPDFNVDKVEKKERRRNSPLPYTTSSMQQDAANKLNFRTRKTMMVAQQLYEGLTLGSQGHQGLITYM
RTDSTRISPVAQSAAHSYIAEQFGEKYSKHGSKVKNASGAQDAHEAIRPSNVFNTPESIAKYLDKDQLKLYTLIWNRFVA
SQMVAAIFDTVKVNLSQNGVIFIANGSQVKFDGYLRIYNDSDKSNMLPEMEENDTVKKANVKPEQHFTQPPARYSEATLI
KTLEENGVGRPSTYAPTLETIQKRYYVILSAKRFEPTELGEIVNKLIVEFFPNIVNTEFTAEMEKDLDQVEEGKRQWIEV
VDQFYKPFAKELENAEEGMEKIQIKDEPAGFDCDVCGSPMVIKLGRFGKFYACSNFPDCRNTKAIVKEIGVKCPLCHEGN
IIERKTKKNRIFYGCDRYPECEFTSWDKPIGRDCPKSGHFLVEKKVRGGGKQVVCSNDECDYQEEKQK
>Mature_707_residues
PTSTKTKTSTKKKTTRKKVTPGKNLVIVESPAKAKTIEKYLGRNYKVVASVGHIRDLKKSTMSVDIENDYEPQYINIRGK
APLINSLKKEAKAAKAVYLASDPDREGEAISWHLAHILNLPLEEKNRVVFNEITKDAVKNAFKEPRQIDVDLVDAQQARR
VLDRLVGYSISPILWKKVKKGLSAGRVQSIALKLIVDRENEINAFIPEEYWSIDGEFKKGTKKFKASFWGIDGKKHPLKN
NEDVIKVMERLDGPDFNVDKVEKKERRRNSPLPYTTSSMQQDAANKLNFRTRKTMMVAQQLYEGLTLGSQGHQGLITYMR
TDSTRISPVAQSAAHSYIAEQFGEKYSKHGSKVKNASGAQDAHEAIRPSNVFNTPESIAKYLDKDQLKLYTLIWNRFVAS
QMVAAIFDTVKVNLSQNGVIFIANGSQVKFDGYLRIYNDSDKSNMLPEMEENDTVKKANVKPEQHFTQPPARYSEATLIK
TLEENGVGRPSTYAPTLETIQKRYYVILSAKRFEPTELGEIVNKLIVEFFPNIVNTEFTAEMEKDLDQVEEGKRQWIEVV
DQFYKPFAKELENAEEGMEKIQIKDEPAGFDCDVCGSPMVIKLGRFGKFYACSNFPDCRNTKAIVKEIGVKCPLCHEGNI
IERKTKKNRIFYGCDRYPECEFTSWDKPIGRDCPKSGHFLVEKKVRGGGKQVVCSNDECDYQEEKQK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=616, Percent_Identity=26.6233766233766, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI4507635, Length=528, Percent_Identity=23.1060606060606, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1787529, Length=752, Percent_Identity=38.031914893617, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI48994931, Length=111, Percent_Identity=39.6396396396396, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788061, Length=543, Percent_Identity=22.6519337016575, Blast_Score=102, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17555378, Length=508, Percent_Identity=24.8031496062992, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32563869, Length=653, Percent_Identity=23.8897396630934, Blast_Score=122, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=553, Percent_Identity=24.0506329113924, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24585251, Length=672, Percent_Identity=22.4702380952381, Blast_Score=143, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 80487; Mature: 80356

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTSTKTKTSTKKKTTRKKVTPGKNLVIVESPAKAKTIEKYLGRNYKVVASVGHIRDLKK
CCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCEEEEEEHHHHHHHHH
STMSVDIENDYEPQYINIRGKAPLINSLKKEAKAAKAVYLASDPDREGEAISWHLAHILN
HHEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEHHHHHHC
LPLEEKNRVVFNEITKDAVKNAFKEPRQIDVDLVDAQQARRVLDRLVGYSISPILWKKVK
CCCCCCCCCHHHHHHHHHHHHHHCCCCEECEEEECHHHHHHHHHHHHCCCCCHHHHHHHH
KGLSAGRVQSIALKLIVDRENEINAFIPEEYWSIDGEFKKGTKKFKASFWGIDGKKHPLK
HCCCCCHHHHEEEEEEECCCCCCCEECCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCC
NNEDVIKVMERLDGPDFNVDKVEKKERRRNSPLPYTTSSMQQDAANKLNFRTRKTMMVAQ
CCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
QLYEGLTLGSQGHQGLITYMRTDSTRISPVAQSAAHSYIAEQFGEKYSKHGSKVKNASGA
HHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
QDAHEAIRPSNVFNTPESIAKYLDKDQLKLYTLIWNRFVASQMVAAIFDTVKVNLSQNGV
HHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCE
IFIANGSQVKFDGYLRIYNDSDKSNMLPEMEENDTVKKANVKPEQHFTQPPARYSEATLI
EEEECCCEEEECCEEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHCCCCCCHHHHHHHH
KTLEENGVGRPSTYAPTLETIQKRYYVILSAKRFEPTELGEIVNKLIVEFFPNIVNTEFT
HHHHHCCCCCCCCCCCHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHH
AEMEKDLDQVEEGKRQWIEVVDQFYKPFAKELENAEEGMEKIQIKDEPAGFDCDVCGSPM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCC
VIKLGRFGKFYACSNFPDCRNTKAIVKEIGVKCPLCHEGNIIERKTKKNRIFYGCDRYPE
EEEECCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEEHHHHCCCCEEECCCCCCC
CEFTSWDKPIGRDCPKSGHFLVEKKVRGGGKQVVCSNDECDYQEEKQK
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHCC
>Mature Secondary Structure 
PTSTKTKTSTKKKTTRKKVTPGKNLVIVESPAKAKTIEKYLGRNYKVVASVGHIRDLKK
CCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCEEEEEEHHHHHHHHH
STMSVDIENDYEPQYINIRGKAPLINSLKKEAKAAKAVYLASDPDREGEAISWHLAHILN
HHEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEHHHHHHC
LPLEEKNRVVFNEITKDAVKNAFKEPRQIDVDLVDAQQARRVLDRLVGYSISPILWKKVK
CCCCCCCCCHHHHHHHHHHHHHHCCCCEECEEEECHHHHHHHHHHHHCCCCCHHHHHHHH
KGLSAGRVQSIALKLIVDRENEINAFIPEEYWSIDGEFKKGTKKFKASFWGIDGKKHPLK
HCCCCCHHHHEEEEEEECCCCCCCEECCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCC
NNEDVIKVMERLDGPDFNVDKVEKKERRRNSPLPYTTSSMQQDAANKLNFRTRKTMMVAQ
CCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
QLYEGLTLGSQGHQGLITYMRTDSTRISPVAQSAAHSYIAEQFGEKYSKHGSKVKNASGA
HHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
QDAHEAIRPSNVFNTPESIAKYLDKDQLKLYTLIWNRFVASQMVAAIFDTVKVNLSQNGV
HHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCE
IFIANGSQVKFDGYLRIYNDSDKSNMLPEMEENDTVKKANVKPEQHFTQPPARYSEATLI
EEEECCCEEEECCEEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHCCCCCCHHHHHHHH
KTLEENGVGRPSTYAPTLETIQKRYYVILSAKRFEPTELGEIVNKLIVEFFPNIVNTEFT
HHHHHCCCCCCCCCCCHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHH
AEMEKDLDQVEEGKRQWIEVVDQFYKPFAKELENAEEGMEKIQIKDEPAGFDCDVCGSPM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCC
VIKLGRFGKFYACSNFPDCRNTKAIVKEIGVKCPLCHEGNIIERKTKKNRIFYGCDRYPE
EEEECCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEEHHHHCCCCEEECCCCCCC
CEFTSWDKPIGRDCPKSGHFLVEKKVRGGGKQVVCSNDECDYQEEKQK
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]