Click here to switch to the map view.

The map label for this gene is gidC

Identifier: 125624095

GI number: 125624095

Start: 1239434

End: 1240780

Strand: Reverse

Name: gidC

Synonym: llmg_1271

Alternate gene names: 125624095

Gene position: 1240780-1239434 (Counterclockwise)

Preceding gene: 125624096

Following gene: 125624087

Centisome position: 49.05

GC content: 38.53

Gene sequence:

>1347_bases
ATGAGAAAAACACATATTAATGTTATCGGAGCAGGCCTTGCCGGCTCTGAAGCCGCTTATCAAATTGCTAAACGCGGTAT
CCCAGTAAAACTTTACGAAATGCGCGGTCTTAAACAGACCCCTCAACATAAAACAGATAAATTTGCTGAACTTGTTTGTT
CAAATTCTCTTCGTGGAGCCGCAATAACCAACGCGGTTGGGCTTCTCAAAGAAGAAATGAGAAGACTCGACTCAGTAATT
ATTAAAGCTGCTGAATACACTCAAGTTCCTGCCGGTGGAGCTCTAGCCGTGGACAGAGAAGGTTTTTCTGATTTTGTTAC
TAAAGAAGTATCTAATCACCCATTAGTTGAGGTTATTCGTGAAGAAATCACTGAAATTCCTCAAGATGAATTAACGATTA
TTGCAACCGGTCCCTTAACTTCTGACAACTTAGCAAATAAAATCCGTGAATTTAATGGCGCTGATGGTTTTTACTTTTAT
GATGCGGCAGCTCCAATCATTGATGCTAATTCGATAAACTTTGATAAAGTTTATAAAAAATCACGTTATGACAAAGGTGA
AGCCGATTATATTAACTGTCCAATGACTAAAGAAGAATTTCAAGCTTTCCAAGAAGCCTTGATTTCTGCAGAAGAAGCTC
CTCTCAATTCTTTTGAGGATTTAAAAGTCTTTGAAGGCTGTATGCCCATTGAAGAAATGGCTAAACGTGGCTATAAAACA
ATGCTGTTTGGTCCCATGAAACCTGTTGGACTGGAATACCCAGATGAATACAAAGGTCCTAGAGATGGCGAATTTAGAAC
ACCTTATGCAGTTGTTCAACTTCGCCAAGATAATGCCTCCGCCTCTTTATATAATATTGTCGGATTTCAAACTCACTTAA
AGTGGGGAGAACAAAAACGAGTTTTCCAAATGATTCCAGGACTCGAAAATGCTGAATTCGTTCGCTACGGTGTAATGCAT
CGAAATTCTTATATGGATTCCCCTAATCTTCTTAAACAAACATTTCAATCACGTAAACAAGAAAATCTCTTTTTTGCTGG
GCAAATGACAGGAGTAGAAGGATATGTTGAATCGGCTGCCTCTGGCTTAGTTGCTGGGATCAATGCGGCTAAACTTTTTA
ATGATGAAGAAGTGGTGATTTTCCCTAAAATAACTGCTATCGGGAGCCTCCCTTACTATATTACCCATACTGATAGCAAA
CATTTCCAACCGATGAATGTCACTTTTGGAATTGTTGAGGAGTTAGATGGTCCACGGATTCGTGATAAGAAAGAACGGTA
TACAAAAGTTGCTGAACGTTCGTTAAATACTTTAACAGATATCATCTCAAAAGAAAATTTAGCATAA

Upstream 100 bases:

>100_bases
GTGACTATCAAGAAGAAAAACAAAAATAAAAGGCGCAATAGCGCTTTTTTATTATATTAATCAATGGTAAAATTCAATAA
AATTAGATATAATTGGGACT

Downstream 100 bases:

>100_bases
TATATAAAAAAACGCTTGAAGCTTATCAAGCGCTTTTTTATATATTACTTTAAAGACTATCTAAGGCATTTTTTTGCTCC
TCATTAATGATATGCGTATA

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MRKTHINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGLKQTPQHKTDKFAELVCSNSLRGAAITNAVGLLKEEMRRLDSVI
IKAAEYTQVPAGGALAVDREGFSDFVTKEVSNHPLVEVIREEITEIPQDELTIIATGPLTSDNLANKIREFNGADGFYFY
DAAAPIIDANSINFDKVYKKSRYDKGEADYINCPMTKEEFQAFQEALISAEEAPLNSFEDLKVFEGCMPIEEMAKRGYKT
MLFGPMKPVGLEYPDEYKGPRDGEFRTPYAVVQLRQDNASASLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMH
RNSYMDSPNLLKQTFQSRKQENLFFAGQMTGVEGYVESAASGLVAGINAAKLFNDEEVVIFPKITAIGSLPYYITHTDSK
HFQPMNVTFGIVEELDGPRIRDKKERYTKVAERSLNTLTDIISKENLA

Sequences:

>Translated_448_residues
MRKTHINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGLKQTPQHKTDKFAELVCSNSLRGAAITNAVGLLKEEMRRLDSVI
IKAAEYTQVPAGGALAVDREGFSDFVTKEVSNHPLVEVIREEITEIPQDELTIIATGPLTSDNLANKIREFNGADGFYFY
DAAAPIIDANSINFDKVYKKSRYDKGEADYINCPMTKEEFQAFQEALISAEEAPLNSFEDLKVFEGCMPIEEMAKRGYKT
MLFGPMKPVGLEYPDEYKGPRDGEFRTPYAVVQLRQDNASASLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMH
RNSYMDSPNLLKQTFQSRKQENLFFAGQMTGVEGYVESAASGLVAGINAAKLFNDEEVVIFPKITAIGSLPYYITHTDSK
HFQPMNVTFGIVEELDGPRIRDKKERYTKVAERSLNTLTDIISKENLA
>Mature_448_residues
MRKTHINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGLKQTPQHKTDKFAELVCSNSLRGAAITNAVGLLKEEMRRLDSVI
IKAAEYTQVPAGGALAVDREGFSDFVTKEVSNHPLVEVIREEITEIPQDELTIIATGPLTSDNLANKIREFNGADGFYFY
DAAAPIIDANSINFDKVYKKSRYDKGEADYINCPMTKEEFQAFQEALISAEEAPLNSFEDLKVFEGCMPIEEMAKRGYKT
MLFGPMKPVGLEYPDEYKGPRDGEFRTPYAVVQLRQDNASASLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMH
RNSYMDSPNLLKQTFQSRKQENLFFAGQMTGVEGYVESAASGLVAGINAAKLFNDEEVVIFPKITAIGSLPYYITHTDSK
HFQPMNVTFGIVEELDGPRIRDKKERYTKVAERSLNTLTDIISKENLA

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Escherichia coli, GI2367273, Length=90, Percent_Identity=43.3333333333333, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17534255, Length=117, Percent_Identity=35.8974358974359, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_LACLM (A2RKQ0)

Other databases:

- EMBL:   AM406671
- RefSeq:   YP_001032578.1
- ProteinModelPortal:   A2RKQ0
- STRING:   A2RKQ0
- GeneID:   4798788
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_1271
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 50201; Mature: 50201

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTHINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGLKQTPQHKTDKFAELVCSNSLRGA
CCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCH
AITNAVGLLKEEMRRLDSVIIKAAEYTQVPAGGALAVDREGFSDFVTKEVSNHPLVEVIR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCCHHHHHHH
EEITEIPQDELTIIATGPLTSDNLANKIREFNGADGFYFYDAAAPIIDANSINFDKVYKK
HHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCHHHHHHH
SRYDKGEADYINCPMTKEEFQAFQEALISAEEAPLNSFEDLKVFEGCMPIEEMAKRGYKT
HCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCEE
MLFGPMKPVGLEYPDEYKGPRDGEFRTPYAVVQLRQDNASASLYNIVGFQTHLKWGEQKR
EEECCCCCCCCCCCHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHH
VFQMIPGLENAEFVRYGVMHRNSYMDSPNLLKQTFQSRKQENLFFAGQMTGVEGYVESAA
HHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHH
SGLVAGINAAKLFNDEEVVIFPKITAIGSLPYYITHTDSKHFQPMNVTFGIVEELDGPRI
HHHHHCCCHHHCCCCCCEEEEECHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCC
RDKKERYTKVAERSLNTLTDIISKENLA
CCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRKTHINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGLKQTPQHKTDKFAELVCSNSLRGA
CCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCH
AITNAVGLLKEEMRRLDSVIIKAAEYTQVPAGGALAVDREGFSDFVTKEVSNHPLVEVIR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCCHHHHHHH
EEITEIPQDELTIIATGPLTSDNLANKIREFNGADGFYFYDAAAPIIDANSINFDKVYKK
HHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCHHHHHHH
SRYDKGEADYINCPMTKEEFQAFQEALISAEEAPLNSFEDLKVFEGCMPIEEMAKRGYKT
HCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCEE
MLFGPMKPVGLEYPDEYKGPRDGEFRTPYAVVQLRQDNASASLYNIVGFQTHLKWGEQKR
EEECCCCCCCCCCCHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHH
VFQMIPGLENAEFVRYGVMHRNSYMDSPNLLKQTFQSRKQENLFFAGQMTGVEGYVESAA
HHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHH
SGLVAGINAAKLFNDEEVVIFPKITAIGSLPYYITHTDSKHFQPMNVTFGIVEELDGPRI
HHHHHCCCHHHCCCCCCEEEEECHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCC
RDKKERYTKVAERSLNTLTDIISKENLA
CCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA