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The map label for this gene is thiE

Identifier: 125624044

GI number: 125624044

Start: 1185836

End: 1186492

Strand: Direct

Name: thiE

Synonym: llmg_1218

Alternate gene names: 125624044

Gene position: 1185836-1186492 (Clockwise)

Preceding gene: 125624043

Following gene: 125624045

Centisome position: 46.88

GC content: 37.44

Gene sequence:

>657_bases
ATGACTAAGAAAACTTTAGATTTAAGTGTTTATTTTATTGCCGGATCACAAAATTTTTCAGAATGTTCACTAGATGAAGC
AACGCAAAAGATTGCTTTAATTATAAAAAGTGGTGTCACTGTTTATCAATTTCGAGATAAGGGAACCATTTATAAAGAGC
AGAAGCAGCGTCTGTCAATTGCTCAAAAATTACAAAAAGTGTCCGAAGAAGCAGGCGTTTCTTTTATTGTCAATGATGAT
GTGGAGTTGGCGCGTGAACTAAATGCTGATGGAATTCATATTGGACAAACAGATGAGTCGGTCAGTAAAGTTCGCGAGAA
GGTTGGTCAGGAAATGTGGTTGGGTCTTTCTGTCACTAATGCTGACGAATTAAAAACGGCACAAAGCAGTGGGGCTGATT
ATTTAGGAATTGGGCCAATTTACCCAACAAACAGTAAAAATGATGCGGCAAAGCCAATTGGCATTAAAGACTTAAGACTG
ATGCTTCTAGAAAATCAATTACCAATTGTAGGAATCGGTGGAATCACGCAAGATTCACTGACAGAATTGTCAGCAATTGG
ACTTGACGGACTTGCGGTTATTTCTTTACTGACAGAAGCAGAAAATCCCAAAAAAGTTGCTCAAATGATTAGACAAAAAA
TCACTAAAAACGGATGA

Upstream 100 bases:

>100_bases
GAAATCATGCCTAATGCGAAAGCTTTTATTAGTGAGGCAATTTCTAAAACGATTATGGTGGGGCATGGTCATGGGCCATT
GAATCATTGGGTGAATATTA

Downstream 100 bases:

>100_bases
CGTACACTTAAAATTACTAAAAATAAATTGTTGCCGTTTTCTTTTCTAAACATGATTTAAACTTACAGAAATGTAAGTTT
TTTTTTCGCTTAATAAATGT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTKKTLDLSVYFIAGSQNFSECSLDEATQKIALIIKSGVTVYQFRDKGTIYKEQKQRLSIAQKLQKVSEEAGVSFIVNDD
VELARELNADGIHIGQTDESVSKVREKVGQEMWLGLSVTNADELKTAQSSGADYLGIGPIYPTNSKNDAAKPIGIKDLRL
MLLENQLPIVGIGGITQDSLTELSAIGLDGLAVISLLTEAENPKKVAQMIRQKITKNG

Sequences:

>Translated_218_residues
MTKKTLDLSVYFIAGSQNFSECSLDEATQKIALIIKSGVTVYQFRDKGTIYKEQKQRLSIAQKLQKVSEEAGVSFIVNDD
VELARELNADGIHIGQTDESVSKVREKVGQEMWLGLSVTNADELKTAQSSGADYLGIGPIYPTNSKNDAAKPIGIKDLRL
MLLENQLPIVGIGGITQDSLTELSAIGLDGLAVISLLTEAENPKKVAQMIRQKITKNG
>Mature_217_residues
TKKTLDLSVYFIAGSQNFSECSLDEATQKIALIIKSGVTVYQFRDKGTIYKEQKQRLSIAQKLQKVSEEAGVSFIVNDDV
ELARELNADGIHIGQTDESVSKVREKVGQEMWLGLSVTNADELKTAQSSGADYLGIGPIYPTNSKNDAAKPIGIKDLRLM
LLENQLPIVGIGGITQDSLTELSAIGLDGLAVISLLTEAENPKKVAQMIRQKITKNG

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=178, Percent_Identity=26.4044943820225, Blast_Score=61, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6325042, Length=233, Percent_Identity=29.1845493562232, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_LACLM (A2RKJ7)

Other databases:

- EMBL:   AM406671
- RefSeq:   YP_001032527.1
- ProteinModelPortal:   A2RKJ7
- SMR:   A2RKJ7
- STRING:   A2RKJ7
- GeneID:   4797017
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_1218
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- ProtClustDB:   CLSK697654
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23698; Mature: 23567

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKTLDLSVYFIAGSQNFSECSLDEATQKIALIIKSGVTVYQFRDKGTIYKEQKQRLSI
CCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHH
AQKLQKVSEEAGVSFIVNDDVELARELNADGIHIGQTDESVSKVREKVGQEMWLGLSVTN
HHHHHHHHHHCCEEEEECCCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHEEEEEECC
ADELKTAQSSGADYLGIGPIYPTNSKNDAAKPIGIKDLRLMLLENQLPIVGIGGITQDSL
HHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHH
TELSAIGLDGLAVISLLTEAENPKKVAQMIRQKITKNG
HHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKKTLDLSVYFIAGSQNFSECSLDEATQKIALIIKSGVTVYQFRDKGTIYKEQKQRLSI
CCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHH
AQKLQKVSEEAGVSFIVNDDVELARELNADGIHIGQTDESVSKVREKVGQEMWLGLSVTN
HHHHHHHHHHCCEEEEECCCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHEEEEEECC
ADELKTAQSSGADYLGIGPIYPTNSKNDAAKPIGIKDLRLMLLENQLPIVGIGGITQDSL
HHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHH
TELSAIGLDGLAVISLLTEAENPKKVAQMIRQKITKNG
HHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA