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The map label for this gene is thiM

Identifier: 125624042

GI number: 125624042

Start: 1184278

End: 1185030

Strand: Direct

Name: thiM

Synonym: llmg_1216

Alternate gene names: 125624042

Gene position: 1184278-1185030 (Clockwise)

Preceding gene: 125624041

Following gene: 125624043

Centisome position: 46.82

GC content: 37.98

Gene sequence:

>753_bases
ATGTCTATTTTGAGTAAGATTCAAAGAACACAACCGCTTATTTTGAACTTGGCGAATTTTGTAACTCCTCAACGAGTCGC
GGACGTTATCAGTTTTATTGGTGCCTCACCTTTAATGACGTCAGAAATTGCTGAGCTCGAGTCATTAGTTGAAATTTCAG
ATGCTGTGGTAGTCAATATTGGCACAATATCAGAATCGACTTATCCTCTTTTTTTAGAAGCTTGTCGCTTGGCAAATCAA
AAAGCAAAACCATTAATTTTGGACCCAGTTGCGGTCAATATTCCTTTCAGAGCATCCATAGTTAAAAGGTTGAGTCAAGA
AGTAAAGTTTAATATTATTCGCGGAAATTCGGCTGAAATTGCTTGGTTTGCTGATAAAAAAAGTTTAAATAAAGGCATTG
ATGCACTTGAGTCAAATATAGATAATGAACACGCGCGACTTGCGGCTAAAAAAACAGGGACAGTGATTATAGAAACAGGT
AAAGTGGATATTATTTCTAACGGTCATGAAGAGATGTATGTTGACACTGACAGCCCACTCTTTAAAATTAATGTTGGTTG
CGGGGACATGCTGACAGCAGTGGTTGGCACCTTTGCAGCTGTTTCCGATGATTTGTTTACAGCTGCTTATGAAGCGACAA
AATTTTTCGGGGAAGCTGGAATGATAGCGACCAAACAAGTGCAAAATTTACCAGGTAATTTTGTCAATAGTCTTCTGGAC
ACACTTTATCAAACGACACACGAAATAAAATAA

Upstream 100 bases:

>100_bases
AATGTGTCCGAGTTTTTATTATATTTTCTTTTGAGAAAATTGTAATTCTCACTTTCTTGTCATGCTTGAGCCCTCCTTGT
GTAAATAAAGGAGTTTTTTT

Downstream 100 bases:

>100_bases
AGGAGTATTACAATGATTACTATTCCACAGGTTGTGACAATTGCTGGAATTGATTCTTCTGGTGGTGCCGGAATTAATGC
GGACATGAAAACTTTTCATA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSILSKIQRTQPLILNLANFVTPQRVADVISFIGASPLMTSEIAELESLVEISDAVVVNIGTISESTYPLFLEACRLANQ
KAKPLILDPVAVNIPFRASIVKRLSQEVKFNIIRGNSAEIAWFADKKSLNKGIDALESNIDNEHARLAAKKTGTVIIETG
KVDIISNGHEEMYVDTDSPLFKINVGCGDMLTAVVGTFAAVSDDLFTAAYEATKFFGEAGMIATKQVQNLPGNFVNSLLD
TLYQTTHEIK

Sequences:

>Translated_250_residues
MSILSKIQRTQPLILNLANFVTPQRVADVISFIGASPLMTSEIAELESLVEISDAVVVNIGTISESTYPLFLEACRLANQ
KAKPLILDPVAVNIPFRASIVKRLSQEVKFNIIRGNSAEIAWFADKKSLNKGIDALESNIDNEHARLAAKKTGTVIIETG
KVDIISNGHEEMYVDTDSPLFKINVGCGDMLTAVVGTFAAVSDDLFTAAYEATKFFGEAGMIATKQVQNLPGNFVNSLLD
TLYQTTHEIK
>Mature_249_residues
SILSKIQRTQPLILNLANFVTPQRVADVISFIGASPLMTSEIAELESLVEISDAVVVNIGTISESTYPLFLEACRLANQK
AKPLILDPVAVNIPFRASIVKRLSQEVKFNIIRGNSAEIAWFADKKSLNKGIDALESNIDNEHARLAAKKTGTVIIETGK
VDIISNGHEEMYVDTDSPLFKINVGCGDMLTAVVGTFAAVSDDLFTAAYEATKFFGEAGMIATKQVQNLPGNFVNSLLDT
LYQTTHEIK

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=249, Percent_Identity=29.3172690763052, Blast_Score=108, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325042, Length=279, Percent_Identity=26.1648745519713, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_LACLM (A2RKJ5)

Other databases:

- EMBL:   AM406671
- RefSeq:   YP_001032525.1
- ProteinModelPortal:   A2RKJ5
- SMR:   A2RKJ5
- STRING:   A2RKJ5
- GeneID:   4798091
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_1216
- eggNOG:   COG2145
- HOGENOM:   HBG351126
- OMA:   TISESTY
- ProtClustDB:   PRK09355
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27192; Mature: 27060

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: BINDING 39-39 BINDING 114-114 BINDING 159-159 BINDING 186-186

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILSKIQRTQPLILNLANFVTPQRVADVISFIGASPLMTSEIAELESLVEISDAVVVNI
CCHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEE
GTISESTYPLFLEACRLANQKAKPLILDPVAVNIPFRASIVKRLSQEVKFNIIRGNSAEI
ECCCCCCHHHHHHHHHHCCCCCCCEEECCEEECCCHHHHHHHHHHHHHEEEEEECCCCEE
AWFADKKSLNKGIDALESNIDNEHARLAAKKTGTVIIETGKVDIISNGHEEMYVDTDSPL
EEEECHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCEEEEECCCCEEEEECCCCE
FKINVGCGDMLTAVVGTFAAVSDDLFTAAYEATKFFGEAGMIATKQVQNLPGNFVNSLLD
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHH
TLYQTTHEIK
HHHHHHHCCC
>Mature Secondary Structure 
SILSKIQRTQPLILNLANFVTPQRVADVISFIGASPLMTSEIAELESLVEISDAVVVNI
CHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEE
GTISESTYPLFLEACRLANQKAKPLILDPVAVNIPFRASIVKRLSQEVKFNIIRGNSAEI
ECCCCCCHHHHHHHHHHCCCCCCCEEECCEEECCCHHHHHHHHHHHHHEEEEEECCCCEE
AWFADKKSLNKGIDALESNIDNEHARLAAKKTGTVIIETGKVDIISNGHEEMYVDTDSPL
EEEECHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCEEEEECCCCEEEEECCCCE
FKINVGCGDMLTAVVGTFAAVSDDLFTAAYEATKFFGEAGMIATKQVQNLPGNFVNSLLD
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHH
TLYQTTHEIK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA