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The map label for this gene is proB [H]

Identifier: 125623729

GI number: 125623729

Start: 856402

End: 857208

Strand: Direct

Name: proB [H]

Synonym: llmg_0885

Alternate gene names: 125623729

Gene position: 856402-857208 (Clockwise)

Preceding gene: 125623728

Following gene: 125623730

Centisome position: 33.86

GC content: 37.3

Gene sequence:

>807_bases
ATGACAAGAAAAAATATTTTGAAAGCTCAGCGAATTGTGGTGAAGATTGGCACGAGTTCCTTGATTTTGCCAAATGGAAA
AATAAATTTATCAAATATTGACGAACTGGCTTTTGTCTTATCTGATTTGAATAATAAAGGTTATGAAGTGATTTTGGTGA
CTTCTGGGGCGATTGGTGTGGGCTTGAATGTCCTTGGAATGGATAAACGTCCAAAAGGAATCGCAGACCAACAGGCTTTG
GCTTCTATCGGTCAGGTCGAATTGATGAGTCTGTATACGCAAATGTTTCGCCGTTATTCACAAAAAGTTTCGCAATTACT
TTTGACTCGTGATGTAACTGATTTTCCAACGAGTCGTGAAAATGCTGAAAATGCATTGAATGCTCTACTTGCACTGGATA
TTATCCCGATTATCAATGAAAATGATGCAATTGCTGTTGATGAAATGGACCATCAAACAAAATTTGGCGATAATGATAAA
CTGGGAGCGATTGTGTCTAAGTTGGTTCATGCCGATTTGTTGATTATGCTTTCAGATATCGATGGACTTTTTGATAAAAA
TCCGACGATTTATGATGATGCTAAAATTTTTAATGAAATTCATGAAATTACTGATGAATTACGCCAAATGGCTGGAGGTG
CCGGTTCTCGTTTTGGTACTGGTGGGATGACTTCGAAATTGGCGGCTGCCCAAATTTTATTTGAAAATGATCAAGAAATG
GTTCTGACCAATGGCGAGCGGATTCGAGAAATTCAACAAATTATCGAAGGACGTGAGATTGGAACATATTTTCATCAAAA
ATTTTGA

Upstream 100 bases:

>100_bases
TTCTTGATAAAATTAGGACCGAAAATAAAATTATAAAAATTTTTAATTTTGTTCGATTTGCTCTCGTATTTGTTGGTATA
TCTTGGTATAATAAGTGATT

Downstream 100 bases:

>100_bases
GGTACTTCTAAAAAGCAAAGCCGTTTTTGCGATGTGACTAGGTGTTTGAGCGACATAGCGAGCATGGTCAGCCTAGCAAA
GCAGGCCACAAAGAAAGAAG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MTRKNILKAQRIVVKIGTSSLILPNGKINLSNIDELAFVLSDLNNKGYEVILVTSGAIGVGLNVLGMDKRPKGIADQQAL
ASIGQVELMSLYTQMFRRYSQKVSQLLLTRDVTDFPTSRENAENALNALLALDIIPIINENDAIAVDEMDHQTKFGDNDK
LGAIVSKLVHADLLIMLSDIDGLFDKNPTIYDDAKIFNEIHEITDELRQMAGGAGSRFGTGGMTSKLAAAQILFENDQEM
VLTNGERIREIQQIIEGREIGTYFHQKF

Sequences:

>Translated_268_residues
MTRKNILKAQRIVVKIGTSSLILPNGKINLSNIDELAFVLSDLNNKGYEVILVTSGAIGVGLNVLGMDKRPKGIADQQAL
ASIGQVELMSLYTQMFRRYSQKVSQLLLTRDVTDFPTSRENAENALNALLALDIIPIINENDAIAVDEMDHQTKFGDNDK
LGAIVSKLVHADLLIMLSDIDGLFDKNPTIYDDAKIFNEIHEITDELRQMAGGAGSRFGTGGMTSKLAAAQILFENDQEM
VLTNGERIREIQQIIEGREIGTYFHQKF
>Mature_267_residues
TRKNILKAQRIVVKIGTSSLILPNGKINLSNIDELAFVLSDLNNKGYEVILVTSGAIGVGLNVLGMDKRPKGIADQQALA
SIGQVELMSLYTQMFRRYSQKVSQLLLTRDVTDFPTSRENAENALNALLALDIIPIINENDAIAVDEMDHQTKFGDNDKL
GAIVSKLVHADLLIMLSDIDGLFDKNPTIYDDAKIFNEIHEITDELRQMAGGAGSRFGTGGMTSKLAAAQILFENDQEMV
LTNGERIREIQQIIEGREIGTYFHQKF

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate 5-kinase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=297, Percent_Identity=32.3232323232323, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI21361368, Length=299, Percent_Identity=32.1070234113712, Blast_Score=122, Evalue=3e-28,
Organism=Escherichia coli, GI1786437, Length=263, Percent_Identity=39.1634980988593, Blast_Score=172, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17569967, Length=276, Percent_Identity=34.7826086956522, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17569969, Length=276, Percent_Identity=34.7826086956522, Blast_Score=147, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6320506, Length=260, Percent_Identity=39.2307692307692, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6321822, Length=272, Percent_Identity=35.6617647058824, Blast_Score=167, Evalue=1e-42,
Organism=Drosophila melanogaster, GI21357643, Length=292, Percent_Identity=34.2465753424658, Blast_Score=141, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 29624; Mature: 29493

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKNILKAQRIVVKIGTSSLILPNGKINLSNIDELAFVLSDLNNKGYEVILVTSGAIGV
CCHHHHHHHHHEEEEECCCEEEECCCCEECCHHHHHHHHHHHCCCCCEEEEEEECCCHHH
GLNVLGMDKRPKGIADQQALASIGQVELMSLYTQMFRRYSQKVSQLLLTRDVTDFPTSRE
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NAENALNALLALDIIPIINENDAIAVDEMDHQTKFGDNDKLGAIVSKLVHADLLIMLSDI
HHHHHHHHHHHHHHHHEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DGLFDKNPTIYDDAKIFNEIHEITDELRQMAGGAGSRFGTGGMTSKLAAAQILFENDQEM
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEE
VLTNGERIREIQQIIEGREIGTYFHQKF
EEECCHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
TRKNILKAQRIVVKIGTSSLILPNGKINLSNIDELAFVLSDLNNKGYEVILVTSGAIGV
CHHHHHHHHHEEEEECCCEEEECCCCEECCHHHHHHHHHHHCCCCCEEEEEEECCCHHH
GLNVLGMDKRPKGIADQQALASIGQVELMSLYTQMFRRYSQKVSQLLLTRDVTDFPTSRE
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NAENALNALLALDIIPIINENDAIAVDEMDHQTKFGDNDKLGAIVSKLVHADLLIMLSDI
HHHHHHHHHHHHHHHHEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DGLFDKNPTIYDDAKIFNEIHEITDELRQMAGGAGSRFGTGGMTSKLAAAQILFENDQEM
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEE
VLTNGERIREIQQIIEGREIGTYFHQKF
EEECCHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]