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The map label for this gene is lacF [H]

Identifier: 125623356

GI number: 125623356

Start: 483966

End: 484841

Strand: Direct

Name: lacF [H]

Synonym: llmg_0489

Alternate gene names: 125623356

Gene position: 483966-484841 (Clockwise)

Preceding gene: 125623355

Following gene: 125623357

Centisome position: 19.13

GC content: 34.13

Gene sequence:

>876_bases
ATGTTTCAAGCTTACGGATTTTTAGCCCCTGCCCTTCTTGTTATTCTCATATTCTTTGTGCTATCAATTTTATTTGCGGT
TTATTTGTCATTTAACAATGTCAATCTTATTGCGCACAATTATCAATGGAATAATTTTAAAAATTACACTAACCTTTTAG
GGGATAAACAACTTTTTCGAGCTTTAGAAAATACAGGTTTCTTTGCTCTAGTTGTGGTGCCAGTTCAAACGATAATCGCT
TTGGTCATCGCCTATATTTTGGCCAATAAAGGCATCAAAGGAAAAACCCTTTTTCGTTTAATTTATTTTTTACCAACATT
GACAAGTTCAGCAGCTTTGACCTTAATTTTCATGTTTATTTTTAATCTAAATGGTCCAATCAATGGTCTCTTAGAAAGTC
TTCATCTGATTAGCCAACCAATTAATTTTATAAATGACACCAATTGGACTTTAAAGGTAATCATGATAATGAATATTTGG
TCAACAGTACCCTATTTTATGACCATTTATCTGGCTTCTTTGGTTGATTTACCTGATTCAATGTATGAAGCCGCTGAAAT
TGATGGGGCGAATGTTTTTGATAAATTACGTTATATCACCATTCCTTATTTGCGGCCAATTACTACTTTTGTTTTACTGA
CAGGGATTATTGGAACTTTTCAAATGTTTGACCAAGCTTATATTTTTTCGGGAGGAACAGGTGGACCTGCAAATAGCACG
CTGACCGTCAGTCTTTTAATCTTTCAATATGCTTTCGGTCAAAATAATCAAATGGGCTACGCAGCAGTCATCGCAATCAT
TTTGGTAATCATCATCTTTATTGTCGCCAGGATTGCTGAGAAATTAAACGGAGGAAATGGTTTTGAAAAGAACTAA

Upstream 100 bases:

>100_bases
AACGTGAAATTTACTGACGAAAGCTTTACAAAGACAAAAAGAAAGGATTTGCTCACATCATATGGAAAAACAAAAAACGA
GTAAAAGAGCCAGAGGCGAG

Downstream 100 bases:

>100_bases
AAAACAAAGTCTGCTTCGGACGCTGCTTTATATTATTTTGATTGCTTATGCTTTTATTACATTTTACCCTTTTATGTGGG
CTTTAGCCGCATCTTTTAAA

Product: sugar transport system permease protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MFQAYGFLAPALLVILIFFVLSILFAVYLSFNNVNLIAHNYQWNNFKNYTNLLGDKQLFRALENTGFFALVVVPVQTIIA
LVIAYILANKGIKGKTLFRLIYFLPTLTSSAALTLIFMFIFNLNGPINGLLESLHLISQPINFINDTNWTLKVIMIMNIW
STVPYFMTIYLASLVDLPDSMYEAAEIDGANVFDKLRYITIPYLRPITTFVLLTGIIGTFQMFDQAYIFSGGTGGPANST
LTVSLLIFQYAFGQNNQMGYAAVIAIILVIIIFIVARIAEKLNGGNGFEKN

Sequences:

>Translated_291_residues
MFQAYGFLAPALLVILIFFVLSILFAVYLSFNNVNLIAHNYQWNNFKNYTNLLGDKQLFRALENTGFFALVVVPVQTIIA
LVIAYILANKGIKGKTLFRLIYFLPTLTSSAALTLIFMFIFNLNGPINGLLESLHLISQPINFINDTNWTLKVIMIMNIW
STVPYFMTIYLASLVDLPDSMYEAAEIDGANVFDKLRYITIPYLRPITTFVLLTGIIGTFQMFDQAYIFSGGTGGPANST
LTVSLLIFQYAFGQNNQMGYAAVIAIILVIIIFIVARIAEKLNGGNGFEKN
>Mature_291_residues
MFQAYGFLAPALLVILIFFVLSILFAVYLSFNNVNLIAHNYQWNNFKNYTNLLGDKQLFRALENTGFFALVVVPVQTIIA
LVIAYILANKGIKGKTLFRLIYFLPTLTSSAALTLIFMFIFNLNGPINGLLESLHLISQPINFINDTNWTLKVIMIMNIW
STVPYFMTIYLASLVDLPDSMYEAAEIDGANVFDKLRYITIPYLRPITTFVLLTGIIGTFQMFDQAYIFSGGTGGPANST
LTVSLLIFQYAFGQNNQMGYAAVIAIILVIIIFIVARIAEKLNGGNGFEKN

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787570, Length=243, Percent_Identity=29.6296296296296, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1789861, Length=277, Percent_Identity=24.5487364620939, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790465, Length=250, Percent_Identity=27.2, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32545; Mature: 32545

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQAYGFLAPALLVILIFFVLSILFAVYLSFNNVNLIAHNYQWNNFKNYTNLLGDKQLFR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCHHHHHH
ALENTGFFALVVVPVQTIIALVIAYILANKGIKGKTLFRLIYFLPTLTSSAALTLIFMFI
HHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHH
FNLNGPINGLLESLHLISQPINFINDTNWTLKVIMIMNIWSTVPYFMTIYLASLVDLPDS
HHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
MYEAAEIDGANVFDKLRYITIPYLRPITTFVLLTGIIGTFQMFDQAYIFSGGTGGPANST
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCH
LTVSLLIFQYAFGQNNQMGYAAVIAIILVIIIFIVARIAEKLNGGNGFEKN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MFQAYGFLAPALLVILIFFVLSILFAVYLSFNNVNLIAHNYQWNNFKNYTNLLGDKQLFR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCHHHHHH
ALENTGFFALVVVPVQTIIALVIAYILANKGIKGKTLFRLIYFLPTLTSSAALTLIFMFI
HHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHH
FNLNGPINGLLESLHLISQPINFINDTNWTLKVIMIMNIWSTVPYFMTIYLASLVDLPDS
HHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
MYEAAEIDGANVFDKLRYITIPYLRPITTFVLLTGIIGTFQMFDQAYIFSGGTGGPANST
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCH
LTVSLLIFQYAFGQNNQMGYAAVIAIILVIIIFIVARIAEKLNGGNGFEKN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]