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The map label for this gene is msmE [H]

Identifier: 125623355

GI number: 125623355

Start: 482525

End: 483790

Strand: Direct

Name: msmE [H]

Synonym: llmg_0488

Alternate gene names: 125623355

Gene position: 482525-483790 (Clockwise)

Preceding gene: 125623354

Following gene: 125623356

Centisome position: 19.08

GC content: 39.65

Gene sequence:

>1266_bases
ATGAAAACTTGGAAAAAAGTTTCGCTCATCAGTCTGACAATGGTCGGCACAATCGGCCTTGTTGCCTGCGGGAATTCGGC
TTCTGGCTCGAAAAATACCCAAACTAAAACACTGACAGTCGGTTATTGGAAAGGCTCAGATACCGAAAATGCGACCTTTG
ACAAATTGGTTAAAAATTTTGAAAAAGCAAATAAAGTTGATGTAAAGCCTAAGGTCTATACGGATATCACGACTCAACTT
CCGACTGATCTCTCTGGTGGGACGGCTCCTGATGTCTTTTACATCGACTCATCTTTCTATCCCTATTTGCAAAAAGAAGG
TGTCTTAAATGACCTGTCAAAAGTTGTCAATAAAGATGATTTTTATTCGTCAATTGCCAAAGCTTTTGAAACGAATGGAA
AAATCTACGCTGCACCAAAAGATGTGTCAACTCTGGCAATCTATGTGAATAAAGACATCTTTGCCAAAGCAGGAATTGAT
ATTAATTCCATTCCAAAATCATATGAAGATTTTATCAAATGGGCGCCAAGCGCTCAAGCCAAGATTGACGCGGTTTATGG
GAAAGGTAAAGTTTATTTGATGAATGTCAATGCTGATTTGACCAGAAACTGGCAATTTATCACAGCTGACGGACAAAACC
CTATCACTAAAGATGGAAAGGCAGATTTATCAAATCCAACCATTTTGAAAAATCTTGGAACAGCCGTTGATTTATTTAAT
ACAGGAGCAGTAGCAACGCCTCAACAAGTTGGGGTGGGTGATGAGGGAGCCGGTTTTGGCAATGGAAAATTTGCCATGGC
GCTGACAGGAAATTGGAATTTCCAAGTTTTTAATAGTCAATACAAAAACCTCCATTATACAATCATTCCTAATTTGACTT
ATCAAGGTAAAAAAATGACCATGCAATACACGGTCGGTTGGGGCGAATACAAAGATACCAAAGCAAAAGCACTTGCTGAC
AAATGGATTCAATATGTCACAGGAAAAGAAGGAATGTCCACCTGGATAAATGGTGTTGGAACACTTGCAACGCGTCCAGA
TGTTGCTGACAGCTCAACTTTCTTAAAAGCCAATCCCTTACTTCAAGTTCATCAAGATTCGATTAAATTTGCAACCCCTT
GGCAAGATGGGACAAATCTGACAACCCTTGTTTCATCGTACGGAAACTTTATTCCAAATGTTTTCAAGAAAGGGGCAACC
GCGGCTGATTTGAAAGCAGCAATGAAACAGGCAGACCAAGATGCCAATAGCAAAATATCAAAATAA

Upstream 100 bases:

>100_bases
AGGGACAAAAGTATGTCTTGAAAAATCAGTTAACAATCAAAATGTGAGATAAAAATATAAATAATTTCACAACATAAAAT
TTCCACTAGGAGGAAAAATC

Downstream 100 bases:

>100_bases
AAGTCATTCTGTCAGTAAAATTTAAGTCCTGTCAGTAAATTTATGCACAAGTAGAAAGCAAAATTTACTGACAGAAACGT
GAAATTTACTGACGAAAGCT

Product: multiple sugar-binding protein precursor

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MKTWKKVSLISLTMVGTIGLVACGNSASGSKNTQTKTLTVGYWKGSDTENATFDKLVKNFEKANKVDVKPKVYTDITTQL
PTDLSGGTAPDVFYIDSSFYPYLQKEGVLNDLSKVVNKDDFYSSIAKAFETNGKIYAAPKDVSTLAIYVNKDIFAKAGID
INSIPKSYEDFIKWAPSAQAKIDAVYGKGKVYLMNVNADLTRNWQFITADGQNPITKDGKADLSNPTILKNLGTAVDLFN
TGAVATPQQVGVGDEGAGFGNGKFAMALTGNWNFQVFNSQYKNLHYTIIPNLTYQGKKMTMQYTVGWGEYKDTKAKALAD
KWIQYVTGKEGMSTWINGVGTLATRPDVADSSTFLKANPLLQVHQDSIKFATPWQDGTNLTTLVSSYGNFIPNVFKKGAT
AADLKAAMKQADQDANSKISK

Sequences:

>Translated_421_residues
MKTWKKVSLISLTMVGTIGLVACGNSASGSKNTQTKTLTVGYWKGSDTENATFDKLVKNFEKANKVDVKPKVYTDITTQL
PTDLSGGTAPDVFYIDSSFYPYLQKEGVLNDLSKVVNKDDFYSSIAKAFETNGKIYAAPKDVSTLAIYVNKDIFAKAGID
INSIPKSYEDFIKWAPSAQAKIDAVYGKGKVYLMNVNADLTRNWQFITADGQNPITKDGKADLSNPTILKNLGTAVDLFN
TGAVATPQQVGVGDEGAGFGNGKFAMALTGNWNFQVFNSQYKNLHYTIIPNLTYQGKKMTMQYTVGWGEYKDTKAKALAD
KWIQYVTGKEGMSTWINGVGTLATRPDVADSSTFLKANPLLQVHQDSIKFATPWQDGTNLTTLVSSYGNFIPNVFKKGAT
AADLKAAMKQADQDANSKISK
>Mature_421_residues
MKTWKKVSLISLTMVGTIGLVACGNSASGSKNTQTKTLTVGYWKGSDTENATFDKLVKNFEKANKVDVKPKVYTDITTQL
PTDLSGGTAPDVFYIDSSFYPYLQKEGVLNDLSKVVNKDDFYSSIAKAFETNGKIYAAPKDVSTLAIYVNKDIFAKAGID
INSIPKSYEDFIKWAPSAQAKIDAVYGKGKVYLMNVNADLTRNWQFITADGQNPITKDGKADLSNPTILKNLGTAVDLFN
TGAVATPQQVGVGDEGAGFGNGKFAMALTGNWNFQVFNSQYKNLHYTIIPNLTYQGKKMTMQYTVGWGEYKDTKAKALAD
KWIQYVTGKEGMSTWINGVGTLATRPDVADSSTFLKANPLLQVHQDSIKFATPWQDGTNLTTLVSSYGNFIPNVFKKGAT
AADLKAAMKQADQDANSKISK

Specific function: Involved in a binding protein-dependent transport system responsible for the uptake of melibiose, raffinose and isomaltotriose [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45910; Mature: 45910

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50222 EF_HAND_2 ; PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTWKKVSLISLTMVGTIGLVACGNSASGSKNTQTKTLTVGYWKGSDTENATFDKLVKNF
CCCCCCEEEEEEEHHHHEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHH
EKANKVDVKPKVYTDITTQLPTDLSGGTAPDVFYIDSSFYPYLQKEGVLNDLSKVVNKDD
HHCCCCCCCCEEEECCHHCCCCCCCCCCCCCEEEECCCCCCHHHHCCHHHHHHHHHCCHH
FYSSIAKAFETNGKIYAAPKDVSTLAIYVNKDIFAKAGIDINSIPKSYEDFIKWAPSAQA
HHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCCEECCCCHHCCCHHHHHHHHCCCCCCC
KIDAVYGKGKVYLMNVNADLTRNWQFITADGQNPITKDGKADLSNPTILKNLGTAVDLFN
EEEEEECCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCEEEHHC
TGAVATPQQVGVGDEGAGFGNGKFAMALTGNWNFQVFNSQYKNLHYTIIPNLTYQGKKMT
CCCCCCCHHCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCEEEEEECCCEECCCEEE
MQYTVGWGEYKDTKAKALADKWIQYVTGKEGMSTWINGVGTLATRPDVADSSTFLKANPL
EEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEECCC
LQVHQDSIKFATPWQDGTNLTTLVSSYGNFIPNVFKKGATAADLKAAMKQADQDANSKIS
EEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
K
C
>Mature Secondary Structure
MKTWKKVSLISLTMVGTIGLVACGNSASGSKNTQTKTLTVGYWKGSDTENATFDKLVKNF
CCCCCCEEEEEEEHHHHEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHH
EKANKVDVKPKVYTDITTQLPTDLSGGTAPDVFYIDSSFYPYLQKEGVLNDLSKVVNKDD
HHCCCCCCCCEEEECCHHCCCCCCCCCCCCCEEEECCCCCCHHHHCCHHHHHHHHHCCHH
FYSSIAKAFETNGKIYAAPKDVSTLAIYVNKDIFAKAGIDINSIPKSYEDFIKWAPSAQA
HHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCCEECCCCHHCCCHHHHHHHHCCCCCCC
KIDAVYGKGKVYLMNVNADLTRNWQFITADGQNPITKDGKADLSNPTILKNLGTAVDLFN
EEEEEECCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCEEEHHC
TGAVATPQQVGVGDEGAGFGNGKFAMALTGNWNFQVFNSQYKNLHYTIIPNLTYQGKKMT
CCCCCCCHHCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCEEEEEECCCEECCCEEE
MQYTVGWGEYKDTKAKALADKWIQYVTGKEGMSTWINGVGTLATRPDVADSSTFLKANPL
EEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEECCC
LQVHQDSIKFATPWQDGTNLTTLVSSYGNFIPNVFKKGATAADLKAAMKQADQDANSKIS
EEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1537846; 12397186 [H]